• Molecular & Cellular Toxicology
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• v.3 no.4
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• pp.273-281
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• 2007

Ceramide is involved in cell death as a lipid mediator of stress responses. In this study, we developed an improved method of ceramide quantification based on added synthetic ceramide and thin layer chromatography (TLC) separation, and applied to biological samples. Lipids were extracted from samples spiked with N-oleoyl-D-erythro-sphingosine ($C_{17}$ ceramide) as an internal standard. Ceramide was resolved by TLC, complexed with fatty-acidfree bovine serum albumin (BSA), and deacylated by ceramidase (CDase). The released sphingosine was derivatized with o-phthalaldehyde (OPA) and measured by high performance liquid chromatography (HPLC). The limit of detection for ceramide was about 1-2 pmol and the lower limit of quantification was 5 pmol. Ceramide recovery was approximately 86-93%. Ceramide concentrations were determined in biological samples including cultured cells, mouse tissues, and mouse and human plasma. TLC separation of ceramide provides HPLC chromatogram with a clean background without any interfering peaks and the enhanced solubility of ceramide by BSAceramide complex leads to the increased deacylation of ceramide. The use of an internal standard for the determination of ceramide concentration in these samples provides an accurate and reproducible analytical method, and this method can be applicable to diverse biological samples.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.4104-4104
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• 2001

Milk somatic cell count (SCC) is a recognized indicator of cow health and milk quality. The potential of near infrared (NIR) spectroscopy in the region from 1100 to 2500nm to measure SCC content of cow milk was investigated. A total of 196 milk samples from 7 Holstein cows were collected for 28 days, consecutively, and analyzed for fat, protein, lactose and SCC. Three of the cows were healthy, and the rest had mastitis periods during the experiment. NIR transflectance milk spectra were obtained by the InfraAlyzer 500 spectrophotometer in a wavelength range from 1100 to 2500 nm. The calibration for logSCC was performed using partial least square (PLS) regression and different spectral data pretreatment. The best accuracy of determination was found for equation, obtained using smoothed absorbance data and 10 PLS factors. The standard error of calibration was 0.361, calibration coefficient of multiple correlation 0.868, standard error of prediction for independent validation set of samples 0.382, correlation coefficient 0.854 and the variation coefficient 7.63%. The accuracy of logSCC determination by NIR spectroscopy would allow health screening of cows, and differentiation between healthy and mastitic milk samples. When the spectral information was studied it has been found that SCC determination by NIR milk spectra was indirect and based on the related changes in milk composition. In the case of mastitis, when the disease occurred, the most significant factors that simultaneously influenced milk spectra were alteration of milk proteins and changes in ionic concentration of milk.

• Journal of Animal Science and Technology
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• v.54 no.6
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• pp.463-469
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• 2012

This study utilized a standardization and cluster analysis technique for the selection and classification of beneficial bacteria. A set of synthetic data consisting of 100 individual variables with three characteristics was created for analysis. The three characteristics assigned to each independent variable were designated to have different numeric scales, averages, and standard deviations. The variables were bacterial isolates at random, and the three characteristics were fermentation products, including cell yield, antioxidant activity of culture, and enzyme production. A standardization method utilizing a standard normal distribution equation to record fermentation yields of each isolate was employed to weight their different numeric scales and deviations. Following transformation, the data set was analyzed by cluster analysis. The Manhattan method for dissimilarity matrix construction along with complete linkage technique, an agglomerative method for hierarchical cluster analysis, was employed using statistical computing program R. A total of 100 isolates were classified into groups A, B, and C. In a comparison of the characteristics of each group, all characteristics in groups A and C were higher than those of group B. Isolates displaying higher cell yield were classified as group A, whereas those isolates showing high antioxidant activity and enzyme production were assigned to group C. The results of the cluster analysis can be useful for the classification of numerous isolates and the preparation of an isolation pool using numerical or statistical tools. The present study suggests that a simple technique can be applied to screen and select beneficial microbes using the freely downloadable statistical computing program R.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.12
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• pp.234-241
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• 2012

This paper proposes a novel design methodology considering transistor layout variation. The proposed design technique is to improve the transistor's electrical characteristics without performing a circuit simulation to extract transistor layout variation. There are three advantages in the proposed method. Firstly, there is no need to change the normal design flow used in layout designs. Secondly, there is no need to perform simulation in order to extract the transistor layout variation. Thirdly, early warnings in layout design lead to decreasing the number of post layout simulations. Less post layout simulations will decrease the number of iterations in the design cycle and shorten design period. The number of bad transistors in the early design phase were reduced from 17.8% to 2.9% by applying eDRC environment for layout designers to develop Standard Cell Library.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.36C no.12
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• pp.27-35
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• 1999

In a typical spread spectrum system, parallel correlator occupies a large fraction of power consumption because of the large number of accumulators in the system. In this paper, a novel accumulator is proposed that can reduce the power consumption in the parallel correlator. The proposed accumulator counts the numbers of 1 of the incoming input data. The counted values are weighted and added together to obtain the final correlation value only at the end of the accumulation. The proposed accumulator has been designed and simulated by CADENCE Verilog-XL and synthesized by SYNOPSYS Design Compiler with $0.6{\mu}m$ standard cell library. Power consumption results have been obtained from EPIC PowerMill simulations. Simulation results are very encouraging. First, the power dissipation is reduced by 22% and the maximum operating frequency is increased by 323%. In addition, the parallel correlator using the proposed accumulators consumed less power than the conventional active parallel correlators by 22%, and less power than the conventional passive correlator by 43%.

• CELLMED
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• v.10 no.4
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• pp.27.1-27.6
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• 2020

Cancer is one of the leading cause of mortality in India as well as worldwide. The management of cancer by conventional therapy has shown life threatening adverse effects. The researchers are now exploring the natural way of treatment. Unani system of medicine have rich literature for cancer and many compound formulations have been described in this system. Unani system of medicine is based on holistic approach and treat human being as a unit with natural herbs, mineral and animal origin drugs. An important compound Unani formulation (CUF) from the literature has been chosen to explore the Unani claim of its anticancer activity. The phytochemical constituents were assessed using standard phytochemical screening method. Antioxidant property of this formulation was assessed by DPPH assay. The DPPH free radical scavenging assay was carried out by colorimetric method and ascorbic acid was taken as a positive control. Three different extracts of CUF on different concentrations were used to screening on human breast cancer (BCC) MCF-7 cell line. For the estimation of in-vitro cytotoxic potency of the investigated extracts was assessed on MTT assay by using trypan blue method and paclitaxel was used as the standard. Hydro-ethanolic (HE) extract showed highest free radical scavenging activity among all extracts. DPPH Assay showed substantial antioxidant activity of these extracts in hydro-ethanol extract at 1㎍ concentration of CUF. The CUF showed antioxidant and anticancer activity. The claim made by Unani physician has been proved.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.5A
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• pp.554-565
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• 2004

In this paper, we implement single cycle and multi cycle adders. We can compare area and time by using the implemented adders. The size of adders is 64, 128, 256-bits. The architecture of hybrid adders is that the carry-out of small adder groups can be interconnected by utilizing n carry propagate unit. The size of small adder groups is selected in three formats - 4, 8, 16-bits. These adders were implemented with Verilog HDL with top-down methodology, and they were verified by behavioral model. The verified models were synthesized with a Samsung 0,35(um), 3.3(V) CMOS standard cell library while a using Synopsys Design Compiler. All adders were synthesized with group or ungroup. The optimized adder for a Crypto-processor included Smart Card IC is that a 64-bit RCA based on 16-bit CLA. All small adder groups in this optimized adder were synthesized with group. This adder can operate at a clock speed of 198 MHz and has about 961 gates. All adders can execute operations in this won case conditions of 2.7 V, 85 $^{\circ}C$.

• Radiation Oncology Journal
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• v.38 no.1
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• pp.11-17
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• 2020

Purpose: Definitive radiotherapy remains a primary treatment option for early stage glottic cancer. Intensity-modulated radiation therapy (IMRT) has emerged as the standard treatment technique for advanced head and neck cancers, whereas three-dimensional conformal radiotherapy (3D-CRT) has remained standard for early glottic cancers. We used the National Cancer Database (NCDB) to identify predictors of IMRT use and effect on outcome in these patients. Materials and Methods: We queried the NCDB from 2004-2015 for squamous cell carcinoma of the glottic larynx staged Tis-T2N0 treated with radiation alone. Logistic regression was used to identify predictors of IMRT. Cox regression was used to identify factors predictive of overall survival. Propensity matching was conducted to account for indication bias. Results: We identified 15,627 patients, of which 11% received IMRT. IMRT use rose from 2% in 2004 to 16% in 2015. Predictors of IMRT include: increased comorbidity, T2 stage, urban location, chemotherapy, treatment at an academic center, and later treatment year. Predictors of improved survival were female gender, higher income, lower stage, no chemotherapy, academic facility, and more remote year. There was no difference in survival between 3D-CRT and IMRT across all stages. Conclusions: The rate of IMRT use for early stage glottic laryngeal cancer has increased over time. There was no difference in outcome in patients receiving IMRT versus 3D-CRT across the cohort.

• Journal of the Korean Wood Science and Technology
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• v.36 no.2
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• pp.46-55
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• 2008

This study assesses the formaldehyde and TVOC emissions from bio-composites with attached fancy veneer manufactured using wood flour and polypropylene (PP) measured using the Field and Laboratory Emission Cell (FLEC) method and 20 L small chamber method. To determine and compare the effects of the adhesive, samples were prepared with different manufacturing methods. In the FLEC result, the formaldehyde emission level of the bio-composites with attached veneer by hot-press was the lowest than pure bio-composite and bio-composite attached veneer using adhesive. The TVOC emission levels are similar to the formaldehyde emission. The TVOC emission level is very low in all of the samples except fancy veneer that is attached with bio-composites using adhesive. The TVOC emission varies depending on how attaching fancy veneer. The results of the 20 L small chamber method were very similar to those obtained with the FLEC, but the correlation was not perfect. However, the FLEC method requires a shorter time than the 20 L small chamber method to measure the formaldehyde and TVOC emissions. The internal bonding strength exceeded the minimum value of $0.4N/mm^2$ specified by the KS standard. All of the bio-composites with attached veneer satisfied the KS standard.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.4A
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• pp.447-457
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• 2004

This paper proposes a RSA crypto-processor for embedded systems. The architecture of the RSA crypto-processor should be used relying on Big Montgomery algorithm, and is supported by configurable bit size. The RSA crypto-processor includes a RSA control signal generator, an optimal Big Montgomery processor(adder, multiplier). We use diverse arithmetic unit (adder, multiplier) algorithm. After we compared the various results, we selected the optimal arithmetic unit which can be connected with ARM core-processor. The RSA crypto-processor was implemented with Verilog HDL with top-down methodology, and it was verified by C language and Cadence Verilog-XL. The verified models were synthesized with a Hynix 0.25${\mu}{\textrm}{m}$, CMOS standard cell library while using Synopsys Design Compiler. The RSA crypto-processor can operate at a clock speed of 51 MHz in this worst case conditions of 2.7V, 10$0^{\circ}C$ and has about 36,639 gates.