• Genomics & Informatics
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• 제21권2호
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• pp.18.1-18.11
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• 2023

Immunologists have activated T cells in vitro using various stimulation methods, including phorbol myristate acetate (PMA)/ionomycin and αCD3/αCD28 agonistic antibodies. PMA stimulates protein kinase C, activating nuclear factor-κB, and ionomycin increases intracellular calcium levels, resulting in activation of nuclear factor of activated T cell. In contrast, αCD3/αCD28 agonistic antibodies activate T cells through ZAP-70, which phosphorylates linker for activation of T cell and SH2-domain-containing leukocyte protein of 76 kD. However, despite the use of these two different in vitro T cell activation methods for decades, the differential effects of chemical-based and antibody-based activation of primary human T cells have not yet been comprehensively described. Using single-cell RNA sequencing (scRNA-seq) technologies to analyze gene expression unbiasedly at the single-cell level, we compared the transcriptomic profiles of the non-physiological and physiological activation methods on human peripheral blood mononuclear cell-derived T cells from four independent donors. Remarkable transcriptomic differences in the expression of cytokines and their respective receptors were identified. We also identified activated CD4 T cell subsets (CD55⁺) enriched specifically by PMA/ionomycin activation. We believe this activated human T cell transcriptome atlas derived from two different activation methods will enhance our understanding, highlight the optimal use of these two in vitro T cell activation assays, and be applied as a reference standard when analyzing activated specific disease-originated T cells through scRNA-seq.

• 대한공간정보학회지
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• 제25권1호
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• pp.71-78
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• 2017

최근 널리 보급되고 있는 태양광 발전소의 유지관리를 위해 다양한 연구들이 시도되고 있다. 본 연구에서는 unmanned aerial vehicle(UAV)기반 열적외선 센서를 이용하여 태양광 셀의 발열을 분석하는 것으로서 주요 결론은 다음과 같다. 먼저 UAV 기반 RGB 센서를 이용하여 정사영상과 digital surface model(DSM) 자료를 구축하였으며, 이를 통해 태양광 셀의 발열 분석에 필요한 태양광 모듈 레이어를 생성하였다. 또한 태양광 모듈 레이어의 위치정확도를 평가하기 위해 virtual reference service(VRS) 측량을 이용하여 검정점에 대한 수평오차를 분석한 결과, 표준오차가 $dx={\pm}2.4cm$, $dy={\pm}3.2cm$로 높은 위치정확도를 확보할 수 있었다. 그리고 태양광 셀의 발열 실험을 위해 고무패치를 설치한 후 UAV 열적외선 센서를 이용하여 발열이 생기는 고무패치의 위치를 효과적으로 분석할 수 있었다. 또한 고무패치 셀 비율과 UAV 열적외선 센서에 의한 셀 비율의 표준오차는 ${\pm}3.5%$로 나타났으며, 따라서 UAV 기반 열적외선 센서를 이용하여 태양광 셀의 발열을 효과적으로 분석할 수 있었다. 아울러 발열이 생기는 셀이 위치하고 있는 태양광 모듈의 코드를 자동으로 추출함으로서 효과적인 태양광발전소 유지보수가 가능하게 되었다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.739-743
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• 2009

Japan is leading standards for the hydrogen fuel quality on the PEMFC(Proton Exchange Membrane Fuel Cell) vehicle at ISO/TC197/WG12. However, it has many problems to solve the standard of measurement and the test method. because the standard of measurement which was proposed in Japan is too high to reach the technique for many countries. it might not control standards for the hydrogen fuel quality on the PEMFC vehicle in Korea. So we need to develop the study of standards and the technique of measurement continuously for the International Standards Meeting.

• 대한치과의사협회지
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• 제51권8호
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• pp.451-458
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• 2013

Human immunodeficiency virus is a retrovirus that causes acquired immunodeficiency syndrome. Acquired immunodeficiency syndrome is defined in terms of "either the occurrence of specific diseases in association with a HIV infection or a CD4 cell count below 200cells/ul" by centers for disease control and prevention(CDC). When performing the surgery of human immunodeficiency virus infected patients, several factors should be considered. First, standard precautions should be performed to prevent infection. It is safe to treat human immunodeficiency virus infected patients if we follow the standard precautions. Second, when making a surgical plan, surgeons have to take account of delayed bone healing and postsurgical infection. This case report presents a case of orthognathic surgery of human immunodeficiency virus infected patient.

• 대한화학회지
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• 제46권5호
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• pp.471-478
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• 2002

• 청정기술
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• 제7권4호
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• pp.243-250
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• 2001

연료전지는 환경 친화적 대체에너지로 지속적인 연구가 이루어지고 있다. 최근에는 연료전지의 실용화를 위해 적층, 대면적화에 대한 기본 기술이 중요시되고 있다. 그러나 연료전지중 가장 많은 기술적 발전을 이룬 인산형 연료전지에 관해서도 연료전지 설계의 기초자료가 되는 스택의 온도 분포에 대한 연구는 거의 발표되지 않았다. 본 연구에서는 인산형 연료전지 스택의 온도 분포를 전산모사하였다. 이를 통하여 여러 작동 조건에서 스택의 온도 분포를 알아내었으며, 스택 운전시 적절한 온도 측정 위치를 예측할 수 있었다. 또한 냉각단의 유로를 변경하여 전산모사를 수행한 결과 스택 내부의 온도 분포의 표준 편차를 약 50% 감소시키는 효과적인 냉각 디자인을 제안할 수 있었다.

• Toxicological Research
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• 제21권1호
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• pp.57-62
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• 2005

To investigate the mutant induction of wild ginseng culture extract, we performed chromosomal aberration assay with chinese hamster lung cell in vitro. The test concentration of the extract was decided for the standard with the 50% suppression of cell propagation in the cell. The concentrations for the chromosome test were 1,250, 2,500 and 5,000 ㎍/ml with metabolic activation (+S, 6 hours treatment), 1,100, 2,200 and 4,400 ㎍/ml without metabolic activation (-S, 6 hours treatment) 800, 1,600 and 3,200 ㎍/ml without metabolic activation (-S, 24 hours treatment). No significant increase in chromosome aberrations was observed at any of these concentrations both in the absence and presence of metabolic activation system. Cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS) caused a significant increase in chromosome aberration. These results may be concluded that wild ginseng culture extract is not capable of inducing chromosome aberration in cultured chinese hamster lung cell regardless of metabolic activation and genotoxicity of that is negative under the present experimental condition.

• The Plant Pathology Journal
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• 제36권5호
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• pp.385-397
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• 2020

The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C₂H₂ TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin-dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.

• Journal of Biosystems Engineering
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• 제32권5호
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• pp.332-338
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• 2007

Yield mapping is necessary for precision farming. An on-site rice weighing system for a head-feed combine was developed to measure the total weight of rice grain harvested while the combine was operated. A load cell system was used to monitor rice weights accumulated into the combine grain tank using a load cell. This method gave cumulative grain weight readings as a function of time. The system consisted of a load cell, two supporting brackets, and a computer-based data acquisition system. The weights measured with the system from two fields were compared with those obtained with a commercially available electronic balance. The response of the load cell to varying grain weights was linearly modeled, showing a coefficient of determination of 0.998 and a standard error of ${\pm}4.09kg$.

• 한국수소및신에너지학회논문집
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• 제22권2호
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• pp.257-264
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• 2011

This study discribes performance of DEFC (Direct Ethanol Fuel Cell) utilized bio-ethanol based on fruit wastes. To produce the bio-ethanol, fruit wastes were treated at temperature $120^{\circ}C$ and 90minutes in acid pre-treatment. After pre-treatment was done, alcohol fermentation process was running. Initial alcohol concentration was 5%. Using the multi coloumn distillation system, more than 95% ethanol was distilled and each component of bio-ethanol was analyzed. In DEFC performance test, it was revealed that cell performance was much higher than that of ethanol. Comparing ethanol with mixed fuel (bio-ethanol (10%) + ethanol (90%)), the performance of ethanol was higher than that of mixed fuel. Even though the bio-ethanol from the fruit wastes is corresponded with transport ethanol standards, it thought that organic matter in bio-ethanol could be negative effect on fuel cell.