• Journal of Internet Computing and Services
• /
• v.25 no.1
• /
• pp.1-8
• /
• 2024

In the API gateway, API composition is an essential function that can reduce the number of client calls and prevent over-fetching and under-fetching. API composition that operate with IMJ (In-Memory Join) consume a lot of resources, putting a burden on the performance of the API gateway. In this paper, to improve the problem of IMJ-style API composition, we propose SAPIC (Stream-based API Composition), which delivers the data to be composed to the client by streaming. SAPIC calls each MSA API that makes up the client response data and immediately streams the received response data to the client, reducing the resource consumption of the API gateway and providing faster response time compared to IMJ. As a result of a comparison experiment with GraphQL, a representative API combination technology, SAPIC recorded a maximum CPU occupancy rate of approximately 21 to 70 % lower, a maximum heap usage rate of approximately 16 to 74 % lower, and a throughput rate that was 1 to 2.3 times higher than GraphQL.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.51 no.3
• /
• pp.89-101
• /
• 2014

Recently, as domestic air traffic dramatically increases, the need of ATC(air traffic control) systems has grown for safe and efficient ATM(air traffic management). Especially, for smooth ATC, it is far more important that performance of display system which should show all air traffic situation in FIR(Flight Information Region) without additional latency is guaranteed. In this paper, we design a ASTERIX(All purpose STructured Eurocontrol suRveillance Information eXchange) parsing module to promote stable ATC by minimizing system loads, which is connected with reducing overheads arisen when we parse ASTERIX message. Our ASTERIX parsing module based on pattern matching creates patterns by analyzing received ASTERIX data, and handles following received ASTERIX data using pre-defined procedure through patterns. This module minimizes display errors by rapidly extracting only necessary information for display different from existing parsing module containing unnecessary parsing procedure. Therefore, this designed module is to enable controllers to operate stable ATC. The comparison with existing general bit level ASTERIX parsing module shows that ASTERIX parsing module based on pattern matching has shorter processing delay, higher throughput, and lower CPU usage.

• Journal of KIISE:Information Networking
• /
• v.35 no.3
• /
• pp.192-206
• /
• 2008

Multihop is the main communication style for wireless sensor networks composed of tiny sensor nodes. Until now, most applications have treated the periodic small sized sensing data. Recently, the burst traffic with the transient and continuous nature is increasingly introduced due to the advent of wireless multimedia sensor networks. Therefore, the efficient communication protocol to support this trend is required. In this paper, we propose a novel PIGAB(Packet Interval Gap based on Adaptive Backoff) protocol to efficiently transmit the burst data in multihop wireless sensor networks. The contention-based PIGAB protocol consists of the PIG(Packet Interval Gap) control algorithm in the source node and the MF(MAC-level Forwarding) algorithm in the relay node. The PIGAB is on basis of the newly proposed AB(Adaptive Backoff), CAB(Collision Avoidance Backoff), and UB(Uniform Backoff). These innovative algorithms and schemes can achieve the performance of network by adjusting the gap of every packet interval, recognizing the packet transmission of the hidden node. Through the simulations and experiments, we identify that the proposed PIGAB protocol considerably has the stable throughput and low latency in transmitting the burst data in multihop wireless sensor networks.

• Journal of Plant Biotechnology
• /
• v.43 no.1
• /
• pp.12-20
• /
• 2016

Advances in DNA sequencing technologies have contributed to revolutionary understanding of many fundamental biological processes. With unprecedented cost-effective and high-throughput sequencing, a single laboratory can afford to de novo sequence the whole genome for species of interest. In addition, population genetic studies have been remarkably accelerated by numerous molecular markers identified from unbiased genome-wide sequences of population samples. As sequencing technologies have evolved very rapidly, acquiring appropriate individual plants or populations is a major bottleneck in plant research considering the complex nature of plant genome, such as heterozygosity, repetitiveness, and polyploidy. This challenge could be overcome by the old but effective method known as haploid induction. Haploid plants containing half of their sporophytic chromosomes can be rapidly generated mainly by culturing gametophytic cells such as ovules or pollens. Subsequent chromosome doubling in haploid plants can generate stable doubled haploid (DH) with perfect homozygosity. Here, classical methodology to generate and identify haploid plants or DH are summarized. In addition, haploid induction by epigenetic regulation of centromeric histone is explained. Furthermore, the utilization of haploid plant in the genomics era is discussed in the aspect of genome sequencing project and population genetic studies.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.11
• /
• pp.7575-7581
• /
• 2015

Corticotropin-releasing factor(CRF), one of the stress driven neuropeptides, was widely proposed to influence hair loss and re-growth. For the development of receptor antagonists, the screening system based on intracellular calcium signal process was developed and optimized. The aequorin parental cells were transfected with CRF1 receptor and alpha 16 promiscuous G protein cDNA to establish HEK293a16/hCRF1, a stable cell line for the human CRF1 receptor. In HEK293a16/hCRF1 cells, the range of sauvagine dose response was 12-fold higher($EC_{50}:15.21{\pm}1.83nM$) than in the transiently expressed cells, hence essential conditions for the antagonist screening experiments such as the robust signals and high solvent tolerance were secured. The standard antagonists for the CRF1 receptor, antalarmin and CP154526, resulted $IC_{50}$ values of $414.1{\pm}5.5$ and $290.7{\pm}1.9nM$, respectively. Similar results were presented with frozen HEK293a16/hCRF1 cells. Finally, our HEK293a16/hCRF1 cells with the aequorin based cellular functional assay can be a model system for the development of functional cosmetics and modulators that can have a clinical efficacy on hair re-growth.

• Microbiology and Biotechnology Letters
• /
• v.42 no.1
• /
• pp.41-50
• /
• 2014

In this study, the performances of PVA-encapsulation and non-encapsulation in a fed-batch bioreactor system were compared for biohydrogen production. Hydrogen production in the PVA-encapsulation bioreactor was not significantly different in comparison to the non-encapsulation bioreactor. However, the hydrogen gas in the encapsulation bioreactor could be stably produced when it was exposed to environmental difficulties such as pH impact by the accumulation of organic acids as fermentative metabolic products. Bacterial communities by DGGE analysis were differently shifted between the PVA-encapsulation and non-encapsulation bioreactors from the initial sludge. The community of hydrogen producing bacteria was stable during the experimental period in the PVA-encapsulation bioreactor compared to the non-encapsulation method. The absolute quantitation of the DNA copy number by a high-throughput droplet digital PCR system for six genera contributed to hydrogen production showing that the numbers of dominant bacteria existed at similar levels in the two bioreactors regardless of encapsulation. In both of two bioreactors, not only Clostridium and Enterobacter, which are known as anaerobic hydrogen producing bacteria, but also Firmicutes, Ruminococcus and Escherichia existed with $1{\times}10^5-1{\times}10^6$ copy numbers of ml-samples exhibiting rapid growth during the initial operation period.

• KSBB Journal
• /
• v.22 no.5
• /
• pp.297-304
• /
• 2007

For large and rapid screening of high-yielding mutants of lovastatin produced by filamentous fungal cells of Aspergillus terreus, one of the most important stage is to test as large amounts of mutated strains as possible. For this purpose, we intended to develop a miniaturized cultivation method using $7m{\ell}$ culture tube instead of traditional $250m{\ell}$ flask (working volume $50m{\ell}$). For obtaining large amounts of conidiospores to be used as inoculums for miniaturized cultures, 4 components i.e., glucose, sucrose, yeast extract and $KH_2PO_4$ were intensively investigated, which had been observed to show positive effect on enhancement of spore production through Plackett-Burman design experimet. When optimum concentrations of these components that were determined through application of response surface method (RSM) based on central composite design (CCD) were used, maximum spore numbers amounting to $1.9\times10^{10}$ spores/plate were obtained, resulting in approximately 190 fold increase as compared to the commonly used PDA sporulation medium. Using the miniaturized cultures, intensive strain development programs were carried out for screening of lovastatin high-yielding as well as highly reproducible mutants. It was observed that, for maximum production of lovastatin, the producers should be activated through 'PaB' adaptation process during the early solid culture stage. In addition, they should be proliferated in condensed filamentous forms in miniaturized growth cultures, so that optimum amounts of highly active cells could be transferred to the production culture-tube as reproducible inoculums. Under these highly controlled fermentation conditions, compact-pelleted morphology of optimum size (less than 1 mm in diameter) was successfully induced in the miniaturized production cultures, which proved essential for maximal utilization of the producers' physiology leading to significantly enhanced production of lovastatin. As a result of continuous screening in the miniaturized cultures, lovastatin production levels of the 81% of the daughter cells derived from the high-yielding producers turned out to be in the range of 80%$\sim$120% of the lovastatin production level of the parallel flask cultures. These results demonstrate that the miniaturized cultivation method developed in this study is efficient high throughput system for large and rapid screening of highly stable and productive strains.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.2
• /
• pp.1200-1206
• /
• 2015

The major target for drug discovery, G-protein coupled receptor (GPCR) is involved in many physiological activities and related to various diseases and disorders. Among experimental techniques relating to the GPCR drug discovery process, various cell-based screening methods are influenced by cell conditions used in the overall process. Recently, the utilization of frozen cells is suggested in terms of reducing data variation and cost-effectiveness. The aim of this study is to evaluate various conditions in cell freezing such as temperature conditions and storage terms. The stable cell lines for calcium sensing receptor and urotensin receptor were established followed by storing cultured cells at $-80^{\circ}C$ up to 4 weeks. To compare with cell stored at liquid nitrogen, agonist and antagonist responses were recorded based on the luminescence detection by the calcium induced photoprotein activation. Cell signals were reduced as the storage period was increased without the changes in $EC_{50}$ and $IC_{50}$ values $EC_{50}:3.46{\pm}1.36mM$, $IC_{50}:0.49{\pm}0.15{\mu}M$). In case of cells stored in liquid nitrogen, cell responses were decreased comparing to those in live cells, however changes by storage periods and significant variations of $EC_{50}/IC_{50}$ values were not detected. The decrease of cell signals in various frozen cells may be due to the increase of cell damages. From these results, the best way for a long-term cryopreservation is the use of liquid nitrogen condition, and for the purpose of short-term storage within a month, $-80^{\circ}C$ storage condition can be possible to adopt. As a conclusion, the active implementation of frozen cells may contribute to decrease variations of experimental data during the initial cell-based screening process.