• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 추계학술대회
• /
• pp.98-98
• /
• 2018

In this study, we evaluated the anti-inflammatory effect of extracts of leaves (ST-L) and branches (ST-B) from Sageretia theezans in LPS-stimulated RAW264.7 cells. ST-L and ST-B significantly inhibited the production of the pro-inflammatory mediators such as NO, iNOS, COX-2, $IL-1{\beta}$ and IL-6 in LPS-stimulated RAW264.7 cells. ST-L and ST-B blocked LPS-induced degradation of $I{\kappa}B-{\alpha}$ and nuclear accumulation of p65, which resulted to the inhibition of $NF-{\kappa}B$ activation in RAW264.7 cells. ST-L and ST-B also attenuated the phosphorylation of ERK1/2, p38 and JNK in LPS-stimulated RAW264.7 cells. In addition, ST-L and ST-B increased HO-1 expression in RAW264.7 cells, and the inhibition of HO-1 by ZnPP reduced the inhibitory effect of ST-L and ST-B against LPS-induced NO production in RAW264.7 cells. Inhibition of p38 activation and ROS elimination attenuated HO-1 expression by ST-L and ST-B, and ROS elimination inhibited p38 activation induced by ST-L and ST-B. ST-L and ST-B dramatically induced nuclear accumulation of Nrf2, but this was significantly reversed by the inhibition of p38 activation and ROS elimination. Collectively, our results suggest that ST-L and ST-B exerts potential anti-inflammatory activity by suppressing $NF-{\kappa}B$ and MAPK signaling activation, and activating HO-1 expression through the nuclear accumulation of Nrf2 via ROS-dependent p38 activation. These findings suggest that ST-L and ST-B may have great potential for the development of anti-inflammatory drug to treat acute and chronic inflammatory disorders.

• 한국식품위생안전성학회지
• /
• 제6권3호
• /
• pp.147-155
• /
• 1991

내열성장독소(ST)를 생산하는 병원성대장균(KS-4, KM-7, KM-12)을 설사돈으로부터 분리하고 몇가지 배양상 특성과 ST생산유전자의 성질을 조사하였다. 분리균은 succinate salts 배지의 pH가 8.5~9.0일 때 ST 생산량이 가장 많았으며, ST정제용 배지로는 succinate salts 배지가 가장 유리한 것으로 생각되어 진다. ST 생산, 축적은 분리균 모두 14~16 시간에서 가장 높았고 균체량은 배양 시작 후 20시간에 가장 많았다. ST 생산 능력이 가장 우수한 KM-7균주로 부터 ST생산 유전을 함유하는 약 80Kbp의 plasmid를 분리하고 EcoRI 제한효소를 절단한 16Kbp의 DNA절편을 pBR 322 vector DNA에 접합시킨 pKD 37 plasmid를 E. coli K-12에 형질전화시켜서 KM-7 보다 ST 생산능력이 우수한 균주(eKT 53)를 얻었다.

• Journal of Acupuncture Research
• /
• 제20권5호
• /
• pp.107-117
• /
• 2003

Objective: This study was examined for effects of acupuncture of Zusanli(ST36) on the facial thermography in health subjects. Methods: The voiunteers who participating in this study had taken rest for 15 mins in room temperature ($19-21^{\circ}C$) before the examination and informed them what to prohibit smoking. drinking and administration of drug for the previous day. The thermography of face was taken using Digital Infrared Thermographic Imaging (D.I.T.I.: Dorex. DTI-16UT1. U.S.A.) by time interval of 35 minutes at 15 min before and 20 min after acupuncture stimuiation. Thermal temperature of Daying(ST5). Juliao(ST3) and Quanliao(SI18) was measured and compared. Results: The results showed that acupuncture of Zusanli(ST36) significantly decreased the Absolute defference of temperature between Daying(ST5) and Juliao(ST3) (p<0.05, Data were represented meant ${\pm}$S.E.M.(n=33)). The results showed that acupuncture of Zusanli(ST36) significantly decreased the Absolute defference of temperature between Quanliao(SI18) and Juliao(ST3)(p<0.01, Data were represented mean${\pm}$S.E.M. (n=33)). But, acupuncture of Zusanli(ST36) particularly decreased the absolute defference of temperature between Daying(ST5) and Quanliao(SI18). Also. it was observed that acupuncture of ST36 decreased variety of the color tone of thermographic contour line and decreased thermal distribution. Conclusions: The results showed that acupuncture of Zusanli(ST36) significantly decreased the absolute defference of temperature of partial facial surface. Hereafter. study about if acupuncture of Zusanli(ST36) significantly decrease the absolute defference of temperature of all the areas of facial surface is requested. Also. continued study of effects of acupuncture of other meridian on the facial thermography is requested.

• Journal of Acupuncture Research
• /
• 제20권6호
• /
• pp.190-200
• /
• 2003

Objective: The purpose of this study is to examine influences by the order, Reduction and Reinforcement in Acupuncture on cerebral hemodynamics [regional cerebral blood flow(rCBF) and mean arterial blood pressure(MABP)] in normal rats. Methods: This experiments was to to investigate eath other changes of rCBF and MABP at Xingjian(LR2)(1st) Shaofu(HT8)(2nd) Reduction, Xingjian(LR2)(1st) Shaofu(HT8)(2nd) Reinforcement, Shaofu(HT8)(1st) Xingjian(LR2)(2st) Reduction and Shaofu(HT8)(1st) Xingjian(LR2)(2st) Reduction in Acupuncture. Results: 1. LR2(1st) HT8(2nd) Reduction in Acupuncture was decreased rCBF and MABP in compared with normal condition. 2. LR2(1st) HT8(2nd) Reinforcement in Acupuncture was significantly decreased rCBF, and was decreased MABP in compared with normal condition. 3. HT8(1st) LR2(2st) Reduction in Acupuncture was decreased rCBF during acupuncture but was recovered rCBF after with-drawing of the neddle. 4. HT8(1st) LR2(2st) Reduction in Acupuncture was decreased MABP during acupuncture and after withdrawing of the neddle. 5. HT8(1st) LR2(2st) Reinforcement in Acupuncture was significantly increased rCBF during acupuncture and 30min after withdrawing of the neddle. 6. HT8(1st) LR2(2st) Reinforcement in Acupuncture was decreased MABP during acupuncture, but was recovered MABP after withdrawing of the neddle. Conclusions: I suggested that LR2 HT8 Reduction in Acupuncture and LR2 HT8 Reinforcement in Acupuncture cause a diverse response of cerebral hemodynas.

• 동의생리병리학회지
• /
• 제22권6호
• /
• pp.1544-1548
• /
• 2008

The aim of this study is to learn the effects of ST36 ST44 and ST25 ST36 electroacupuncture stimulation on high fat diet-induced obese mouse. The control group was provided with high fat diet without electroacupuncture stimulation, while the experimental group received a electroacupuncture stimulation on ST36 ST44 and ST25 ST36 with three frequencies (10Hz, 100Hz, 200Hz) for 5 times a week during five week periods with the high fat diet. We investigated the body weight, weight gain, and food intake. After the electroacupuncture stimulation, a significant inhibition of increase in weight was found in the experimental group compared with the high fat diet group. But there were no significant differences made by the change of acupoint combinations and frequencies. After the electroacupuncture stimulation, no significant change was found in the food intakes, but the weight gain a week was decreased compared with the high fat diet group. Electroacupuncture stimulation may be effective in treatments of obesity to high fat diet, But no significant difference was found between three frequencies.

• 한국가축번식학회지
• /
• 제27권3호
• /
• pp.269-274
• /
• 2003

본 연구는 과배란처리에 의한 수정란이식 시 우수한 수정란을 다량 확보하고 이식 후 수태율 향상을 위하여 수란우에 rbST처리가 수태율 및 progesterone 농도와 황체의 크기에 어떠한 영향을 미치는가를 조사하고자 실시하였다. 공란우는 Folltropin-V와 PGF$_2$$\alpha$를 이용하여 과배란처리를 유도하여 12시간 간격으로 2 straw씩 3회 인공수정을 실시하였다. 공란우와 수란우는 대조구와 rbST 처리구로 구분하였으며, rbST (500 mg)처리는 발정발현 후 미근부에 근육 주사하였다. 과배란처리된 공란우의 수정란채란은 수정 후 7∼8일째에 비외과적인 방법으로 실시하였다. 수정란이식 후 수태율은 rbST 처리구에서 대조구보다 유의적으로 높았다 (64.0 vs. 47.1%; p<0.05). 채취한 혈액의 progesterone 농도 분석과 황체의 단면적를 조사한 결과 progesterone 농도는 대조구와 rbST 처리구에서 6일 동안 차이가 없었지만, 6일 이후부터 차이가 나타났으며 황체크기 역시 rbST 처리구에서 대조구에 비해 높게 나타났다. 본 연구결과에서 rbST처리는 이식 후 수태율의 향상시킬 수 있을 것으로 판단된다.

• 한국발생생물학회지:발생과생식
• /
• 제2권1호
• /
• pp.39-43
• /
• 1998

스테로이드 호르몬의 합성은 콜레스테롤로부터 시작되고 Steroidogenic acute regulatory protein(StAR)은 스테로이드의 합성과정에서 콜레스테롤을 미토콘드리아의 안으로 신속하게 운반하는 역할을 한다. 스테로이드 호르몬은 난소, 부신, 고환에서 합성되며 본 연구에서는 흰쥐 난소와 부신에서 StAr mRNA의 발현 양상을 in situ hybridization 기법을 이용하여 조사하였다. 난소의 경우 StAR mRNa는 프로게스테론을 분비하는 황체에서 강한 발현을 보였고 엔드로겐을 분비하는 난포막세포에서도 약한 발현을 보였으며 에스트로겐을 분비하는 과립막세포에서는 발현되지 않았다. 황체에서도 황체의 발달 저도에 따라 차이를 보였고 성장한 황체에서는 강한 발현을 보인 반면, 퇴화하는 황체에서는 약한 발현을 보였다. 부신에서 StAR mRNA는 피질에서 강한 발현을 보였고 수질에서는 발현되지 않았다. 특히, 피질의 토리층에서보다 다발층과 그물층에서 강한 발현을 보였다. 난소와 부신에서 StAR mRNA는 스테로이드 호르몬의 종류에 따라서 발현 양상이 달랐고 스테로이드 호르몬 합성의 초기 단계에 관여함을 알 수 있었다.

• 한국식품위생안전성학회:학술대회논문집
• /
• 한국식품위생안전성학회 1994년도 추계 학술 심포지움
• /
• pp.15-26
• /
• 1994

Bovine somatotropin(bST) or bovine growth hormone (bGH) is a protein of 191 amino acids produced by the anterior pituitary gland of cattle. Recombinant bovine somatotropin(rbST) is biosynthetic versions of the naturally occurring pituitary hormone in cows. The use of rbST in dairy cows promises to improve the efficiency of milk production around the world. Using recombinant DNA technology, bST can now be produced in commercial quantities. The recombinant bST(rbST) is biologically identical to the found in the bovine pituitary. Milk from rbST-treated cows has been found to have the same nutritional value and composition as milk from untreated cows. In November of 1993, rbST finally was approved by the FDA, nearly 10 years after filing a licence applica-tion. rbST has been one of the most extensively studied animal drug products to be reviewed by the agency. Three scientific facts will help to reassure the public about the safety of the milk suppy.: 1. rbST has no biological activity in humans when indigested orally or when given by intramuscular injection. 2. Insulin-like growth factor 1(IGF-1) is not orally active. Any changes in IGF-1 levels in milk are well within normal variation and are lower than those reported in human milk. 3. All cow's milk contains bST, and no significant change in bST levels in milk occurs as a result of giving cows supplemental bST. Based on the scientific evidence, the public can be confident that milk and meat from rbST-treated cows is safe to consumers.

• Journal of Animal Science and Technology
• /
• 제64권3호
• /
• pp.515-530
• /
• 2022

Sequence type (ST) 5 methicillin-resistant Staphylococcus aureus (MRSA) with staphylococcal cassette chromosome mec (SCCmec) type II (ST5-MRSA-II) and ST72-MRSA-IV represent the most significant genotypes for healthcare- (HA) and community-associated (CA) MRSA in Korea, respectively. In addition to the human-type MRSA strains, the prevalence of livestock-associated (LA) MRSA clonal lineages, such as ST541 and ST398 LA-MRSA-V in pigs and ST692 LA-MRSA-V and ST188 LA-MRSA-IV in chickens, has recently been found. In this study, clonotype-specific resistance profiles to cathelicidins derived from humans (LL-37), pigs (PMAP-36), and chickens (CATH-2) were examined using six different ST groups of MRSA strains: ST5 HA-MRSA-II, ST72 CA-MRSA-IV, ST398 LA-MRSA-V, ST541 LA-MRSA-V, ST188 LA-MRSA-IV, and ST692 LA-MRSA-V. Phenotypic characteristics often involved in cathelicidin resistance, such as net surface positive charge, carotenoid production, and hydrogen peroxide susceptibility were also determined in the MRSA strains. Human- and animal-type MRSA strains exhibited clonotype-specific resistance profiles to LL-37, PMAP-36, or CATH-2, indicating the potential role of cathelicidin resistance in the adaptation and colonization of human and animal hosts. The ST5 HA-MRSA isolates showed enhanced resistance to all three cathelicidins and hydrogen peroxide than ST72 CA-MRSA isolates by implementing increased surface positive charge and carotenoid production. In contrast, LA-MRSA strains employed mechanisms independent of surface charge regulation and carotenoid production for cathelicidin resistance. These results suggest that human- and livestock-derived MRSA strains use different strategies to counteract the bactericidal action of cathelicidins during the colonization of their respective host species.

• 대한핵의학회:학술대회논문집
• /
• 대한핵의학회 2000년도 2nd Korea-China Congress of Nuclear Medicine and 39th Annual Spring Meeting if the Korea SOciety Nuclear Medicine
• /
• pp.77.2-77.2
• /
• 2000