• Journal of Acupuncture Research
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• v.20 no.5
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• pp.133-150
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• 2003

Objective: Recently. many studies have showed the evidences of the effect of the Electro-acupuncture treatment through scientific. Methods : One of these methods is functional MRI. We performed electro-acupuncture on Zusanli(St36) and observed the change of brain activation using fMRI. Zusanli(St36) is located on the lateral side of the lower leg. 3 cun(寸) inferior to the Patella of the lower border. Theoretically and clinically. this point has been considered very important for gynecological disorders. spleen and stomach disorders. and psychological disorders. To see the effects of electro-acupuncture stimulation on Zusanli(St36). the experiment was carried out on twelve healthy volunteers. using the gradient echo sequence with the 3.0T whole-body fMRI system(ISOL). After the needle insertion on right Zusanli(St36). 2 Hz of electric stimulation was given for 30 seconds. repeated five times. with 30 seconds' intervals. The Image analysis including motion correction. talairach transformation, and smoothing was done with SPM99. Results ad conclusion : The electro-acupuncture stimulation on Zusanli(St36) activates Brodmann Area 6, 13, 2, 19, 21, 22, 39, 40, 38, 3which indicates the pathways of the electro-acupuncture stimulation on Zusanli(St36) and the possibility of the relationship of the electro-acupuncture stimulation on Zusanli(St36) with autonomic nervous system, internal organic system.

• Journal of Korean Medicine Rehabilitation
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• v.24 no.3
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• pp.1-9
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• 2014

Objectives The aim of this study was to investigate the effects of acupuncture on weight loss, food intake, lipid metabolism, adipogenesis. Methods Four week-old C57BL/6J mice acclimatized to the laboratory environment for 1 week were allocated into four groups of 6~8 mice each: normal diet group, high fat diet group, high fat diet group and treated with acupuncture at HT7, high fat diet group and treated with acupuncture at ST36 for 90days. High fat diet group was used to control group. Results 1) Body weight, food intake of the ST36, HT7 groups decreased compared with those of control group. ST36 group was more effective with body weight decrease, and HT7 group was more effective with food intake decrease. 2) NEFA, TG, TC, ALT, AST of the ST36, HT7 groups decreased compared with those of control group. ST36 group was more effective. 3) The expression of SREP-1c, SREBP-2 of the ST36 group decreased compared with those of control group. These decreased rates were statistically significant. SREBP-2 of the HT7 group decreased significantly compared to the control group. 4) LXR, FAS, SCD of the ST36 group decreased significantly compared with control group. 5) p-ACC, HMGCR of the ST36 group decreased significantly compared with those of control group. HMGCR of the HT7 group decreased significantly compared with control group. Conclusions These results suggest that ST36, HT7 acupuncture may be used prevent or treat the obesity induced by high fat diet.

• BMB Reports
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• v.30 no.2
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• pp.95-100
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• 1997

Two kinds of cDNA encoding mouse $Gal{\beta}$1,3(4)GlcNAc ${\alpha}$2,3-sialyltransferase (mST3Gal III) and $Gal{\beta}$1,4(3)GlcNAc ${\alpha}$2,3-sialyltransferase (mST3Gal IV) were isolated from mouse brain cDNA library by means of a PCR-based approach. The cDNA sequences included an open reading frame coding for proteins of 374 and 333 amino acids, respectively, and the primary structure of these enzymes suggested a putative domain structure consisting of four regions, like that in other glycosyltransferases. The deduced amino acid sequences of mST3GaI III and IV showed a 98% and 89% identity with rat ST3GaI III and human ST3Gal IV, respectively. Northern analysis indicated that the expression of mST3Gal III mRNA was abundant in heart, liver and adult brain, while that of mST3GaI IV mRNA was detected in all tissues tested except for testis, but the level was the highest in liver. Soluble forms of mST3GaI III and IV transiently expressed in COS cells exhibited enzyme activity toward acceptor substrates containing the terminal either $Gal{\beta}$1,3GlcNAc or $Gal{\beta}$1,4GlcNAc sequences. The substrate preferences of both enzymes were stronger for tetrasaccharides than for disaccharides.

• Polymer(Korea)
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• v.34 no.2
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• pp.89-96
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• 2010

Adhesive binders with core-shell structure of organic/organic pair were prepared by emulsion polymerization of acrylic monomers, such as methyl methacrylate(MMA), ethyl acrylate(EA), n-butyl acrylate(BA), and styrene(St). Ammonium persulfate (APS) was used as an water soluble initiator in the presence of an anionic surfactant, sodium dodecyl benzene sulfonate (SDBS). Non-woven fabric and leather were impregnated with the adhesive binder. The surface of the impregnated fabric and leather were treated with plasma technique and then kinetics analysis and mechanical properties were measured. The conversions of the polymerization of core-shell binder (MMA/EA, MMA/BA) were greater than 90%. When the core-shell binder was prepared at equimolar conditions, the increasing effect of the core-shell binder on the state peel strength of the impregnated and plasma-treated non-woven/non-woven fabric has the order of MMA/St, EA/BA, BA/MMA, EA/St, and EA/MMA. When the core-shell binder was prepared at non-equimolar conditions, the increasing effect of the core-shell binder on the state peel strength of the non-woven fabric/leather has the order of MMA/BA, BA/EA, MMA/EA, St/MMA, and EA/St.

• Journal of Preventive Medicine and Public Health
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• v.23 no.1 s.29
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• pp.106-116
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• 1990

This study was conducted to investigate of water qualities in the upper and middle reaches of the Han River. For this purpose, water was sampled at Kwangjin and 1st Han-River Bridges of the Han River in Seoul and analysed from August, 1988 to September, 1989. The results are summarized as follows : 1. Water quality at 1st Han-River Bridge was more polluted than that at Kwangjin Bridge. 2. Except biological oxygen demand (BOD), turbidity, suspended solid (SS), dissolved oxygen (DO), DO saturation (DOS), ammonia nitrogen ($NH_3-N$), nitrite nitrogen ($NO_2-N$) and chloride ion ($Cl^-$) at Kwangjin and 1st Han-River Bridges were lower as compared with the previous data before redevelopment of the Han River. 3. SS, DO and pH at Kwangjin and 1st Han-River Bridges could be classified to the 1st grade in environmental water quality standard. DOS at Kwangjin Bridge was over 100% and that at 1st Han-River Bridge was below 100% in the Han River. BOD at Kwangjin Bridge could be classified to End grade and that at 1st Han-River Bridge to 3rd grade in environmental water quality standard. 4. The higher the level of water was, the lower the levels of turbidity and SS, and $NH_3-N$ was decreased with increasing water level at 1st Han-River Bridge. DO was decreased as water temperature went up but DOS was increased with DO. BOD was positively correlated with nitrite-nitrogens. 5. Turbidity and SS at the both sites and Chloride ion ($Cl^-$) at Kwangjin Bridge were increased in July and August. And DO at the both sites and $NH_3-N$ at 1st Han-River Bridge were decreased in at July and August.

• Journal of Pharmacopuncture
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• v.12 no.4
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• pp.77-88
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• 2009

Objectives : The purpose of this study was to compare the effect of acpuncture and electro-acupuncture of low(EA(L)) and high(EA(H)) frequency at Haegye(ST41) on intestinal hypomotility induced with loperamide in rats. Methods : We made suppressed state of intestinal motility with loperamide in rats and carried out needle retention acupuncture, low frequency electro-acupuncture and high frequency electro-acupuncture at ST41 in rats devided into pre-treatment group and post-treatment group. We fed charcoal to them after the treatment and measured the travel rate of charcoal in the gastrointestinal track to analyze which treatment is more effective in state of intestinal hypomotility. Results : None of acupuncture, EA(L) and EA(H) at ST41 had significant influences on intestinal motility of rat in normal state. Needle retention at ST41 did not significantly increase intestinal motility suppressed with loperamide in rats. Pre-treatment of EA(L) and EA(H) at ST41 significantly increased intestinal motility suppressed with loperamide in rats. Post-treatment of EA(L) and EA(H) at ST41 did not have significant influences on intestinal motility of rat in normal state. Conclusions : These results suggest that treatment of EA(L) and EA(H) at ST41 may be effective on gastric disorders such as intestinal hypomotility and its effect had more prevention than cure. Further study is necessary to know more effects of ST41 and electro-acupuncture of low and high frequency.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.974-981
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• 2020

Sequence type 410 (ST410) of Escherichia coli is an extraintestinal pathogen associated with multi drug resistance. In this study, we aimed to investigate the horizontal propagation pathway of a high-risk clone of E. coli ST410 that produces Klebsiella pneumoniae carbapenemase (KPC). blaKPC-encoding E. coli and K. pneumoniae isolates were evaluated, and complete sequencing and comparative analysis of blaKPC-encoding plasmids from E. coli and K. pneumoniae, antimicrobial susceptibility tests, polymerase chain reaction, multilocus sequence typing, and conjugal transfer of plasmids were performed. Whole-genome sequencing was performed for plasmids mediating KPC-2 production in E. coli and K. pneumoniae clinical isolates. Strains E. coli CPEc171209 and K. pneumoniae CPKp171210 were identified as ST410 and ST307, respectively. CPEc171209 harbored five plasmids belonging to serotype O8:H21, which is in the antimicrobial-resistant clade C4/H24. The CPKp171210 isolate harbored three plasmids. Both strains harbored various additional antimicrobial resistance genes. The IncX3 plasmid pECBHS_9_5 harbored blaKPC-2 within a truncated Tn4401a transposon, which also contains blaSHV-182 with duplicated conjugative elements. This plasmid displayed 100% identity with the IncX3 plasmid pKPBHS_10_3 from the K. pneumoniae CPKp171210 ST307 strain. The genes responsible for the conjugal transfer of the IncX3 plasmid included tra/trb clusters and pil genes coding the type IV pilus. ST410 can be transmitted between patients, posing an elevated risk in clinical settings. The emergence of a KPC-producing E. coli strain (ST410) is concerning because the blaKPC-2-bearing plasmids may carry treatment resistance across species barriers. Transgenic translocation occurs among carbapenem-resistant bacteria, which may spread rapidly via horizontal migration.

• Journal of Environmental Science International
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• v.10 no.3
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• pp.225-231
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• 2001

The fish species collected in the Keumho River basin are 42 species 31 genera belonging to 15 Families. This report was investigated for the evaluation of water quality in the Keumho River system which is a tributary of Nakdong River in Korea on september in 1999. The fishes collected were 42 species, 31 genera belonging to 15 Families. The dominant species were 5 species; Zacco platypus, Zacco temmincki, Squalidus chankaensis tsuchigae, Moroco oxycephalus, Squalidus gracilis majimae, and 8 species; Hemibarbus longirostris, Pseudogobio esocinus, Culter brevicauda, Cobitis rotundicaudata, Pseudobagrus fulvidraco, Pungitius sinensis kaibarae, Monopterus albus, Channa argus were rare species. The relationship among the GPI, EC and BOD by the organic pollutants were over 0.9. The group pollution index(GPI) was lowest at St. 1(0.85) and highest at St. 1(0.85) and highest at St. 5(2.33). The water quality of the Keumho river divided into 3 parts; the water of upper reaches in river(St. 1) was 1st class(oligotrophic condition), middle parts(St. 2, 3, 4) were 3rd class($\alpha$-mesosaprobic condition) and lower part(St. 5) was 4th class(Polysaprobic condition) as the source of tap water, respectively. And the tributary which are the Sinryeong Stream(St. 6), the Sincheon(St. 7) and the Donghwa Stream(St. 9) in Keumho river were 2nd class as the source of tap water. The results in this study was represented same patterns as the result by the use of indicator species like as algae and invertebrates for the discrimination of water quality. So, some freshwater fish species can be use applicant for the discrimination of water quality.

• Clinical and Experimental Pediatrics
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• v.58 no.6
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• pp.206-210
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• 2015

Purpose: The use of a 12-week steroid regimen (long-term therapy, LT) for the first episode of idiopathic nephrotic syndrome (NS) reportedly induces a more sustained remission and lower relapse rate than previous regimens, including an 8-week steroid regimen (short-term therapy, ST). Here, we assessed the potential for selective application of 2 steroid regimens (LT vs. ST) based on the days to remission (early responders [ER] vs. late responders [LR]) for the first idiopathic NS episode in children. Methods: Patients were divided into 4 subgroups (ST+ER, ST+LR, LT+ER, and LT+LR) according to the initial steroid regimen used and rapidity of response; the baseline characteristics, relapse rates, and cumulative percentage of children with sustained remission were then compared among the 4 subgroups. Results: Fifty-four children received ST, and the remaining 45 children received LT. As observed in previous studies, children receiving LT showed significantly lower relapse rates during the first year after the first NS episode than those receiving ST. The ST+ER group showed significantly lower relapse rates during the first one year and two years after the first NS episode than the the ST+LR group, whereas there were no significant differences of the relapse rates and duration to the first relapse between the ST+ER and LT+ER groups. Conclusion: We suggest that the initial steroid regimen in idiopathic NS patients can be shortened according to the duration to remission i.e., LT in patients achieving remission after the first week of steroid therapy, and ST in those achieving remission within the first week of steroid therapy.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.2
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• pp.488-493
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• 2006

To make a objective diagnosis of Dam Hun(痰暈), we investigated the 63 patients with Dam Hun(痰暈) and 34 normal people as control group. we compared the thermal difference between Chon-jung(CV17 ) and Chung-wan(CV12 中脘), Chon-jung(CV17 ) and Gwan-won(CV4 關元) and we compared the thermal difference of Yang-mun(ST21 梁門), Chun_chu(ST25 天樞), Su-do(ST28 水道), too. All 63 patients had dizziness and GI trouble. They had the symptom - fatigue, nausea vomitting, head heaviness, indigestion, cold limbs, headache, heart burn, constipation, diarrhea, anorexia. And some had edema, numbness, insomnia, palpitation, facial flushing, dysuria, ringing. Considering the average temparature of Chon-jung(CV17 ), Chung-wan(CV12 中脘), Gwan-won(CV4 關元), Yang-mun(ST21 梁門), Chun-chu(ST25 天樞), Su-do(ST28 水道) between the patients group and the control group, the patients group were lower than the control group except for Chon-jung(CV17 ), Gwan-won(CV4 關元), but the statistical significance was not. Considering the thermal difference of Chon-jung(CV17 ) and Chung-wan(CV12 中脘), Gwan-won(CV4 關元), Yang-mun(ST21 梁門), Chun-chu(ST25 天樞), Su-do(ST28 氷道) between the patients group and the control group, the temparature deviation of Chon-jung(CV17 ) and Chung-wan(CV12 中脘), Chon-jung(CV17 ) and Yang-mun(ST21 梁門) was significant. We suggest that coldness of upper abdomen is significant in diagnosis of Dam Hun(痰暈).