• Journal of the East Asian Society of Dietary Life
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• v.9 no.2
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• pp.195-199
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• 1999

The characteristics of protease from squid viscera was investigated by response surface methodology(RSM) programmed with reaction temperature and pH. The optimal temperature and pH for the protease were 41.75$^{\circ}C$ and pH 6.02 respectively. Also its activity was 78.65 unit at the optimal condition and $R^2$ of the model was 0.8461 (P＜0.1). The protease activity was decreased by N $a^{+}$ and increased by $Mg^{2+}$ But $K^{+}$ did not affect the protease. The Km value against casein was determined to be 0.12 mM by Line-weaver-Burk plot.lot.