• 한국컴퓨터정보학회논문지
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• 제20권1호
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• pp.29-37
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• 2015

Nowadays, the user-centric application based web 2.0 has replaced the web 1.0. The users gain and provide information by interactive network applications. As a result, traditional approaches that only extract and analyze users' local document operating behavior and network browsing behavior to build the users' preference profile cannot fully reflect their interests. Therefore this paper proposed a preference analysis and indicating approach based on the users' communication information from MicroBlog, such as reading, forwarding and @ behavior, and using the improved PersonalRank method to analyze the importance of a user to other users in the network and based on the users' communication behavior to update the weight of the items in the user preference. Simulation result shows that our proposed method outperforms the ontology model, TREC model, and the category model in terms of 11SPR value.

• Applied Science and Convergence Technology
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• 제24권1호
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• pp.1-8
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• 2015

Protein immobilization on a gold surface plays an important role in the usefulness of biosensors that utilize gold-coated surfaces such as surface plasmon resonance (SPR), quartz crystal microbalance (QCM), etc. For developing high performance biosensors, it is necessarily required that immobilized proteins must remain biologically active. Loss of protein activity and maintenance of its stability on transducer surfaces is directly associated with the choice of immobilization methods, affecting protein-protein interactions. During the past decade, a variety of strategies have been extensively developed for the effective immobilization of proteins in terms of the orientation, density, and stability of immobilized proteins on analytical devices operating on different principles. In this review, recent advances and novel strategies in protein immobilization technologies developed for biosensors are briefly discussed, thereby providing an useful information for the selection of appropriate immobilization approach.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.630-633
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• 2003

Transfer of an electron from one site to another in a molecular or between molecules and/or electrodes is one of the most fundamental and ubiquitous processes in chemistry, biology and physics. In this study fusion proteins composed by green fluorescent protein(GFP) and cytochrome b562 were used in fabricating molecular array as an electron sensitizer and electron acceptor, Protein formation onto the substrate was performed by the self-assembly technique. The fusion protein film were analyzed using scanning probe microscope(SPM), Surface Plasmon Resornance(SPR) and hybrid STM/I-V. The results suggest that the proposed molecular photodiode can be used as a basic unit of the memory device.

• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.221-226
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• 2012

DNA-functionalized silver and silver/gold bimetallic nanoparticle superstructure probes with controllable sizes and optical properties are synthesized using monothiol DNA and dithiothreitol. The superstructures exhibit a very narrow size distribution, which can be easily controlled by balancing the ratio of dithiothreitol and DNA. These superstructures assemble reversibly in a highly cooperative manner, and are SERS active. Multiplexed colorimetric detection of DNA targets using these superstructure probes has been demonstrated to identify three different DNA target sequences that are associated with three lethal diseases, respectively.

• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 Proceedings of the second conference of aseanian membrane society
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• pp.65-68
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• 2004

Molecular imprinting, which was first proposed by Wulff and Sarhan in 1972 [1], is a facile way to construct molecular recognition sites by applying a simple radical polymerization [2]. Since 1994, the authors have proposed an alternative molecular imprinting method in which polymeric materials are directly converted into molecular recognition materials [3].(omitted)

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 춘계학술발표대회
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• pp.242.2-242.2
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• 2011

Molecularly imprinted polymer thin films were applied to develop a gas sensor based on the surface plasmon resonance phenomenon for small gaseous molecules such as toluene and xylene. The imprinted polymer films were synthesized via photo-polymerization method using various combination of templates, functional monomers and cross-linkers. The temperature of pre-polymerization solutions and the power of UV light were controlled for optimized performance of gas sensing. The morphology and porosity of the polymer films were controlled by varying the mixing ratios of the pre-polymerization solutions and confirmed by atomic force microscopy. By fitting the adsorption/desorption sensorgrams to conventional kinetic models, the effects of different templates and cross-linkers were interpreted in term of the structural differences of the polymer networks formed on the gold film. The sensitivity and selectivity of sensors were estimated for toluene and xylene, and also for humidity and other gaseous molecules such as formaldehyde and ammonia.

• 전자공학회논문지S
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• 제34S권9호
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• pp.50-59
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• 1997

This paper is on the generalized singular perturbation approximation (GSPA) preserving the discrete positive real property. We transform the discrete positive real(PR) system into a stochastically banlanced system and get the reduced order discrete system from the GSPA of the full order stochastically balanced system. eSPECIALLY, WHEN THE FREE PARAMETER OF THE gspa IS .+-.1, we show that the reduced order discrete system retains stability, minimality, and positive real and stochstically balancing properties. And we derived the .inf.-norm error bound with the reduced order discrete strictly positive real(SPR) system by the proposed method. Finally, we give an example to ascertain the properties of the proposed reduced order discrete system and to compare with the conventional methods.

• KSBB Journal
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• 제17권2호
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• pp.121-136
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• 2002

Immunosensors are of great interest because of their potential utility as specific, simple, label-free, direct detection means and provision of reduction in size, cost and time of analysis comparing with conventional immunoassay. In the last two decades, many reports have been published on the use of immunosensors for a wide range of applications to clinical diagnostics, pharmaceutical chemistry, environmental monitoring, biotechnology and food industries. There are also numerous transduction techniques developed such as electrochemical techniques, piezoelectric crystal, and surface plasmon resonance receiving much attention for the direct monitoring of immune reactions at solid surfaces. In this article, the principles, characteristics, structures, fonctions and clinical applications of immunosensors were reviewed

• 대한전기학회논문지:시스템및제어부문D
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• 제52권3호
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• pp.148-155
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• 2003

In this paper, a nonlinear anisotropic filtering method without the loss of important information happened due to the repeated filtering in magnetic resonance images is proposed. First of all original images are divided into four regions, e.g., SPR(Strong Plain Region), EPR(Easy Plain Region), SER(Strong Edge Region), and EER(Easy Edge Region). An optimal template among multiple templates is selected, then the nonlinear anisotropic filtering based on the template is applied in pixel by pixel basis. In the proposed algorithm, filtering strength of EER containing important information is adjusted very weak and filtering strength for remaining regions is also adjusted according to the degree of the importance. In spite of repeated filtering, resulting images by the proposed method could still preserve anatomy information of original images without any degradation. Compared to the existing nonlinear anisotropic filtering, the proposed filtering method with multiple templates provides higher reliability for filtered images.

• 산업경영시스템학회지
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• 제32권2호
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• pp.179-185
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• 2009

공공사업이나 자동화 생산시스템 등에 있어서 설비 자산이 차지하는 비중은 매우 크며 이러한 설비들의 경제적 설비 대체 분석을 위하여 설비에 대한 수명분석이 이루어져야 한다. 본 논문에서는 설비들의 폐기 자료가 불충분한 경우의 수명분석에서 Iowa형 생존곡선을 기초로 한 CM 방법을 제시하였다. 기존의 SPR 방법이 평균 수명만을 추정하는데 비하여 제시된 CM 방법은 설비의 수명분포를 추정함으로써 평균 수명뿐만 아니라 폐기 자료의 폐기곡선과 폐기율을 추정할 수 있어 설비 교체 분석 시 유용하게 사용될 수 있다.