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Influence of Boron and Iron Toxicity on the Physiological Status, Growth, and Mineral Uptake of Ginseng in Hydroponic Culture (인삼 수경재배 시 붕소와 철 과잉 농도가 인삼의 생리장해 증상, 생육 및 무기원소 흡수에 미치는 영향)

  • Yu, Jin;Kang, Soo Hyun;Jang, In Bae;Jang, In Bok;Park, Ki Choon;Lee, Ueong Ho;Park, Hong Woo;Suh, Su Jeoung;Seo, Tae Cheol;Kim, Kee Hong
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.3
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    • pp.175-182
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    • 2017
  • Background: Ginseng is a perennial crop grown for more than four years in the same place. Therefore, it is highly affected by the soil environment, especially nutrients in the soil. The present study was carried out to investigate to the influence of boron and iron concentrations on the physiological status, growth, and mineral uptake of ginseng to obtain the basic information for diagnosing a physiological disorder in ginseng plants. Methods and Results: The boron and iron concentrations were controlled at 3, 30, 150, 300 and 2, 20, 100, $200mg/{\ell}$, respectively. When treated with $150mg/{\ell}$ of boron, the ginseng plants showed yellowing or necrosis symptoms at the edge or end of their leaves. Compared with the $3mg/{\ell}$ treatment, the root weight decreased by 13 and 24% in the 150 and $300mg/{\ell}$ treatments, respectively. When treated with $20mg/{\ell}$ of iron, the ginseng plants showed yellowing between the veins of the leaves followed by the formation of brown spots. The root weight gradually decreased with increasing iron concentration. Approximately 55% decrease in root weight was observed upon treatment with $200mg/{\ell}$ of iron. Conclusions: The boron toxicity occurs in the leaves of ginseng at the boron concentration of approximately 1,900 mg/kg or more. The iron toxicity occurs at the iron concentration of approximately 120 mg/kg for leaves and 270 mg/kg for roots.

Inhibitory Effect of Methanol Extracts and Solvent Fractions from Doenjang on Mutagenicity Using in vitro SOS Chromotest and in vivo Drosophila Mutagenic System (된장 메탄올 추출물 및 분획물에 의한 in vitro SOS Chromotest 실험계와 in vivo 초파리 돌연변이 검출계에서의 항돌연변이 효과)

  • Lim, Sun-Young;Lee, Sook-Hee;Park, Keun-Young;Yun, Hee-Sun;Lee, Won-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.9
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    • pp.1432-1438
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    • 2004
  • This study investigated the inhibitory effect of methanol extracts and several solvent fractions from doen-jang on mutagenicity using in vitro SOS chromotest and in vivo Drosophila mutagenic system. In order to determine an antimutagenic effect of doenjang methanol extracts, other soybean fermented foods and original materials were compared. The treatment of doenjang methanol extracts (100 ${\mu}$/assay) to SOS chromotest system inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 87~97% and showed higher antimutagenic effect than other fermented foods. Among solvent fractions from doenjang methanol extracts, the ethylacetate and dichloromethane fractions showed the stronger antimutagenic effect (91% and 95%, respectively) in SOS chromotest. In Drosophila mutagenic system, the treatment of ethylacetate fraction (5%/bottle) significantly inhibited aflatoxin $B_1$ induced mutagenicity by 97%. These results demonstrated that doenjang had an inhibitory effect to mutagenic agents in both in vitro and in vivo mutagenic systems, suggesting that its antimutagenic effect may be due to active compounds in the ethylacetate fraction from doenjang methanol extracts.

Assessment of sediment and total phosphorous loads using SWAT in Oenam watershed, Hwasun, Jeollanam-do (SWAT 모델을 이용한 외남천 유역의 토사 및 총인 유출량 분석)

  • Lee, Taesoo
    • Journal of the Korean association of regional geographers
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    • v.22 no.1
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    • pp.240-250
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    • 2016
  • Monitoring for water quantity and quality was conducted in this study for 2 years (2012~2013) in Oenam Stream which is a tributary of Seomjin River and upstream of Juam Lake. Suspended solid and total phosphorous(TP) were monitored and analyzed, then water quantity and quality as well as their relation with landuses were identified based on the previous study. Flow showed the similar pattern with precipitation but some discrepancies existed due to the distance between weather station(Gwangju) and study area. Watershed was modeled based on observed data using SWAT(Soil and Water Assessment Tool). Model calibration was conducted using data obtained in 2012 and validation was conducted using data in 2013. The coefficient of determination ($R^2$) between observed and modeled showed 0.6644 and 0.5176 for flow and TP, respectively for model calibration period. For validation period, $R^2$ was 0.7529 for flow and 0.7057 for TP, which were higher than calibration period. Hot spots were determined for watershed management by analyzing the amount of sediment and TP outcome from each sub-watershed. TP loading by landuse determined that cropland, of which the area takes only 5% from entire watershed, generated 53.6% of TP and residential and cowshed was responsible for 23.5% of TP loading.

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Phosphorylated Proteins of Mitogen Stimulated-Rat Peripheral Blood Lymphocytes (분열유발인자에 의한 흰쥐 림프구 단백의 인산화)

  • Jou, I-Lo;Ko, Sung-Soo;Ahn, Young-Soo
    • The Korean Journal of Pharmacology
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    • v.29 no.1
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    • pp.121-130
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    • 1993
  • This study was done to classify the proteins involved in the specific phosphorylation using the rat peripheral blood lymphocytes (rPBL) stimulated with mitogens, phorbol 12-myristate 13-acetate (PMA) and concanavalin A (Con A). The lymphocytes were incubated with $^{32}P-orthophosphate$ before PMA or Con A stimulation. The migration patterns of the phosphorylated proteins of mitogen-treated rPBL in two dimensional electrophoretic fields were analyzed after autoradiography. The stimulation of the lymphocytes with PMA and Con A increased the phosphorylation of thirteen protein fractions. The phosphorylation intensities of the protein spots differ to the treatments of the cells with specific kinase inhibitors, H-7 and W-7. These protein fractions were grouped into 3 classes, namely, PKC-mediated, CaM kinase-mediated, and other kinase mediated proteins. The effect of the duration of the stimulation on the phosphorylated behaviors occurred concurrently, not sequentially, although each individual protein fraction had a different time for the peak phosphorylation during the stimulation period upto 30 minutes. The phosphoproteins found in the cytosolic soluble fraction were phosphorylated prior to those in the pellet, whose phosphorylations were sustained at a high level for over 10 minutes. The above results suggest that the early events in lymphocyte activation involve 3 different sets of proteins which are phosphorylated by CaM kinase, PKC and other kinases, and those kinases do not work sequentially, but rather, independently or cooperatively.

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Monitoring of pesticide residues at alpine and sloped-land in Gangwondo, Korea (강원도 고랭지 배추경작지의 토양 및 수질 중 농약 오염 실태)

  • Park, Dong-Sik;Kim, Tae-Han;Kim, Seong-Soo;Lee, Sang-Min;Kim, Song-Mun;Hur, Jang-Hyun
    • The Korean Journal of Pesticide Science
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    • v.8 no.3
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    • pp.189-197
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    • 2004
  • Alpine and sloped-land in Gangwondo, Korea is the most important land type for cultivation of Chinese cabbage. However, farmers in these regions have major problems with insect pests, weeds and disease. Over use or inappropriate use of agrochemicals occurs frequently. No intensive study of pesticide contamination in this area has been done. The work presented in this paper addresses this deficiency. We measured pesticide residues within soil and water samples using multiresidue analysis. Samples were collected bimonthly from April to October, 2002 at three sites with to sampling spots. At the three sites, Pyeongchang, Jeongseon and Taebaeck, pesticides most frequently detected (>30% of samples) in soil samples were endosulfan, fluazinam, diniconazole, alachlor, prothiofos and dimethomorph. The amount of pesticide residues in the soils was ranged from 0.004 to $0.412\;mg\;kg^{-1}$ in these samples. Non-registered pesticides were also detected in these samples, indicating illegal use of pesticides. No pesticide were detected in the water samples collected from those sites. The results showed that pesticide residues might be dependant on physiochemical properties of pesticides, application history and soil properties. This study provides basic data for appropriate pesticide use on alpine and sloped-land in Korea.

Development of Augmented Reality(AR)-based Tourism Contents using Local Cultural Resources (지역 문화유산을 활용한 증강현실 기반 관광 콘텐츠 개발)

  • Lee, Jongwook;Park, Hyun-Ah;Park, Kang-Ah
    • The Journal of the Korea Contents Association
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    • v.18 no.6
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    • pp.122-130
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    • 2018
  • As smart tourism is gaining more attention, there are increasing attempts to make use of augmented reality(AR) in tourism industry. However, existing AR-based tourism contents are only limited to the 'designated' cultural heritage, overlooking the value of undesignated cultural heritage lost in the past few decades of development era. This hinders expanding the scope of tourism contents and leads to the production of banal and similar contents. Given that, our study has developed tourism contents based on , an AR-based mobile application that enables participants to virtually visit the historical sites lost in the development era. The Chungmuro area, which the app is named after, is unique in character. The area has been famous for movie making for over 30 years until the '90s. It is abundant with movie-related cultural resources such as theaters and print shops, which makes the place where people wants to feel the glorious past. Through the app, participants are able to walk along the historic trail and take pictures at five virtual historic spots, among many others. We also examined participants' level of satisfaction and overall tourism experience. By taking the result into account, we hope that the AR-based tourism contents will increase in the future.

Comparative Studies of Seven Solvents for the Extraction of Neutral Lipid in Corn Embryo (옥수수 배의 중성 지질의 추출을 위한 7종 용매의 비교 연구)

  • 김덕진;전영민
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.6
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    • pp.596-602
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    • 1991
  • The comopositon of lipids extracted from corn embryo with various solvents were analyzed. The solvents for the extraction were benzene(BZ), n-hexane(HX), pet. ether(PE), trichlorethylene (TE), chloroform-methoanol(2:1, v/v) (CM), dichlormethane - methanol(2:1, v/v)(DM) and hexanediethyl ether(5:1, v/v)(HD). The lipids were than fractinated by silicis acid column chromatography(SACC) into three lipid fractions. The Neutral lipid fractons were further separated by thin layer chromatography(TLC) and the individual lipid spots were quantitatived by TLC scanner. And then the fatty acid compositions of total lipids and neutral lipids were determined by gas chromatography(GC). Crude oil contents of corn embryo were most efficient by using DM, CM and neutral lipid was extracted much HX, BZ, HD systems than did PE, DM, DM an CM were most efficient solvent systems for extracting glycolipid and phospholipid. The major component of the neutral lipid fractions was found to be triglyceride, and it was superior DM to PE. Linoleic acid was the predominant fatty acid in the total lipids, and it was most efficient with BZ and TE. The major fatty acid in neutral lipids was also linoleic acid and it was superior BZ to PE, CM, HD and oleic acid was similar to seven solvents and palmitic acid was found much superior in using CM.

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A Study on the Key Sucess factors Analysis Based on the Malcolm Baldridge National Quality Award (Malcolm Baldridge National Quality Award의 평가모델에 근거한 핵심성공요인분석)

  • Jung, Kyung-Hee;Cho, In-Hee;Kim, Sung-Je;Cho, Jai-Rip
    • Journal of the Korea Safety Management & Science
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    • v.10 no.4
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    • pp.297-307
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    • 2008
  • At the spots of enterprises experiencing rapid environmental changes, necessity for ceaseless managemental innovation is raised. We can't deny that management quality is also a part of such managemental innovation. Introduction of management quality by an organization is different from introduction of other individual innovative techniques. Management quality is not one time project but complete historical change program to be pursued continuously. Furthermore, it must be understood as a strategic 'Quality Journey' looking for far future of an organization. Malcolm Baldrige Model has been increasingly used in Korea as a global standard for management quality. The model, however, has shown a number of limitations in application due to nonsystematic elements in the implementation process. The deployment process of the model should be detailed through a real world case study. In this research, we proposed a quality evaluation standard model which is suitable for the Korean public enterprise based on the Malcolm Baldrige Non-profit Criteria, and examined the model. A 7-Point Likert Scale was used based on the seven categories within the 2007 Malcolm Baldrige Non-profit Criteria: Leadership, Strategic Planning, Customer and Market Focus, Management Analysis and Knowledge Management, Workforce Focus, Process Management, and Results. Furthermore, we analysed the validity and causal relationship among the factors within the model. Through the above case study, following common factors for successful Malcolm Baldrige Model have been derived. The following is a summary of the results of this study. First, for Malcolm Baldrige Model management, needs powerful support Leadership. Second, for Malcolm Baldrige Model management, needs systematic Strategic Planning. Third, for Malcolm Baldrige Model management, needs the continuous Customer and Market Focus. Fourth, for Malcolm Baldrige Model management, needs systematic Measurement Analysis and Knowledge Management. Five, for Malcolm Baldrige Model management, needs systematic Workforce Focus. Six, for Malcolm Baldrige Model management, needs systematic Process Management. The finding are surely necessary factors to arrange the human resource management for upgraded management quality, those factors help to explain why all enterprises consider human resource as the strategic view.

Halo Spot Symptom Induced by Oviposition of Frankliniella occidentalis on Grape Fruits: Molecular Diagnosis by a Species-specific DNA Amplification and Microscopic Characterization of the Symptom (꽃노랑총채벌레 산란에 의한 포도 과피 달무리 반점: 종 특이적 분자진단법을 이용한 종동정과 반점 증상의 형태적 특징)

  • Ahn, Seung-Joon;Cho, Myoung Rae;Park, Cheol Hong;Kang, Taek Jun;Kim, Hyung Hwan;Kim, Dong-Hwan;Yang, Chang Yeol
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.281-286
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    • 2014
  • In grape vineyards, whitish spots in a cloud shape have been often observed on the fruit surface recently. However, the cause of the halo spot symptom was unknown, hindering countermeasures to be properly designed for the control. A small hole in the middle of the formless halo spot remained as a scar formed by oviposition of the thrips. It became later a suberized scab, which is separated from the epidermal cells on the surface either to be retained on or to be detached from it as time proceeds. Such a symptom is distinguished from the feeding damages caused by thrips or true bugs occurring on the grape fruits. With DNA extracted from the egg-shell found in the hole, molecular diagnosis by amplifying an ITS2 region with universal primers and subsequently digesting the PCR product by an restriction enzyme (RsaI) revealed that the egg was laid by Frankliniella occidentalis. In addition, a mitochondrial COI sequence confirmed that the halo spot symptom was formed by its oviposition. This study provides accurate information on the peculiar damage symptom caused by oviposition of F. occidentalis that could be useful in the control strategies for this pest in vineyards.

Predicting Potential Distribution of Monochamus alternatus Hope responding to Climate Change in Korea (기후변화에 따른 솔수염하늘소(Monochamus alternatus) 잠재적 분포 변화 예측)

  • Kim, Jaeuk;Jung, Huicheul;Park, Yong-Ha
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.501-511
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    • 2016
  • Predicting potential spatial distribution of Monochamus alternatus, a major insect vector of the pine wilt disease, is essential to the spread of the pine wilt disease. The purpose of this study was to predict future domestic spatial distribution of M. alternatus by using the CLIMEX model considering the temperature condition of the vector's life history. To predict current distribution of M. alternatus, the administrative divisions data where the pine wilt spots caused by M. alternatus were found from 2006 to 2014 and the 10-year mean climate observed data in 68 meteorological stations from 2006 to 2015 were used. Eight parameter sets were chosen based on growth temperature range of M. alternatus reported in preceding researches. Error matrix method was utilized to select and simulate the parameter sets showing the highest correlation with the actual distribution. Regarding the future distribution of M. alternatus, two periods of 2050s(2046-2055) and 2090s(2091-2100) were predicted using the projected climate data of RCP 8.5 Scenario generated from Korea Meteorological Administration. Overall results of M. alternatus distribution simulation were fit in the actual distribution; however, overestimation in Seoul Metropolitan area and Chungnam Region were shown. Gradual expansion of M. alternatus would be expected to nationwide from western and southern coastal areas of Korea peninsula.