• Title/Summary/Keyword: spots

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Simultaneous Identification/Determination System for Sildenafil, Homosildenafil, Vardenafil and Tadalafil as Adulterants in Foods (식품중 불법함유된 발기부전치료제 성분 검출을 위한 동시분석법 연구)

  • Jang, Jae-Hee;Park, Kun-Sang;Park, Hye-Kyung;Koo, Yong-Eui;Choi, Youn-Ju;Hwang, In-Kyung;Kim, Dai-Byung
    • Journal of Food Hygiene and Safety
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    • v.18 no.4
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    • pp.195-201
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    • 2003
  • An easily available, simultaneous identification/determination procedure for sildenafil, homosildenafil, tadalafil, vardenafil in adulterated health related foods was established by using a combination of three different analytical methods; thin layer chromatography(TLC), liquied chromatography-mass spectrometry (LC/MS) and high-performance liquied chromatography (HPLC)/photo-diode-array detector. The sample solution for TLC was applied to silica gel 60 $F_{254}$ plates with ethylacetate/acetonitrile/25%ammonia (90:10:5) as a developing solvent. Spots were located under UV radiation at 254 nm and dragendolfs reagent. Mass spectra of the compounds by LC/MS were investigated with electrospray ionization (ESI) interface, under positive ion mode. The HPLC analysis was performed on a column of capcell pack $C_{18}$ (UG120, 4.6${\times}$250mm I.D. 5 ${\mu}$m)with 0.1% sodium 1-hexansulfonate (in 0.1% phosphoric acid)/acetnitrile (73:27) as a mobile phase, and effluent was minitored with a photo-diode-applied to commercial foods, Sildenafil content was inthe range of 0.4mg/g~360.9 mg/g from 7 out of 35 samples. Homosildenafil content was in the range of 2.2 mg/g~336.0 mg/g from 7 out of 35 samples. Tadalafil content was 429.3 mg/g, 9.6 mg/500 mg from 2 out of 35 samples. The procedure described here is available for the screening of sildenafil, homosildenafil, tadalafil, vardenafil.

A Study on Application of GPS for Deflection Management of Curved PCT Girder Bridge under Construction (시공 중 곡선형 PCT 거더교의 처짐 관리를 위한 GPS 적용 연구)

  • Kyu Dal, Lee;Jin Duk, Lee
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.33 no.5
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    • pp.453-461
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    • 2015
  • In order to manage the deflection of a curved PCT girder bridge during construction, a GPS receiver was installed at the spot predicted to be the weak point during the incremental launching so as to measure the deflection at each construction stage. The deflections obtained in the experiment were compared with those derived from the monitoring of stress, temperature and inclination. The comparative analysis of the GPS measurement and analytical values obtained from finite element modeling with respect to the launching distance showed that the measured values differ by 0.6 to 1.6 times to the analytical results. This difference could be significantly reduced by thermal calibration. From the analysis of the behavioral pattern of the bridge, deflection occurred during construction in the concrete tip due to the deflection at the head of the nose at the 95m and 75m-spots, and compression and tension developed respectively at the compression weak zone and tension weak zone. The application of GPS appeared to enable more efficient management of the deflection during the erection of the curved PCT girder bridge and is expected to be helpful for the prediction and management of the behavior in future ILM construction sites.

Ecological and Morphological Characteristics of Zostera caulescens Miki (Zosteraceae) in Korea (한국산 수거머리말(Zostera caulescens Miki.)의 형태 및 생태적 특성에 대한 연구)

  • Lee, Sang-Yong;Suh, Young-Bae;Kim, Sang-Tae;Choi, Chung-Il
    • Ocean and Polar Research
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    • v.24 no.4
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    • pp.345-357
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    • 2002
  • Ecological characteristics on habitats and morphological features of the seagrass Zostera caulescens Miki in Korea were examined. The biogeographical distribution of Z. caulescens was confirmed in the south coast of the Korea. Zostera caulescens usually inhabits at the inner bay, where is sheltered from wave action and 3.0 to 6.5 m deep. The sediment in habitats is composed of very fine muddy sand or sandy mud. In morphology, Z. caulescens is easily distinguished from other species of the genus by the formation of vegetative canopy on the top of reproductive stems. The number of longitudinal ribs in testa was 24 revealed by scanning electron microscope (SEM) while the number of ribs in Z. marina has been often reported to be 16-20. Purplish anthocyanin spots were displayed on the surface when the testa was removed. We found that the size and shape of leaf epidermal cells in Z. caulescens were very different from those of Z. marina when the leaf cuticles were removed by maceration which could be useful characters for identifying Zostera species. The leaf of Z. caulescens displays two different casts of color when the fresh plant is closely observed. The margins of leaves appear brighter than the center of leaves due to the thickening by the development of paralleled venation in the middle of leaves. The comparison of two populations of Z. caulescens in Korea showed that they were considerably different in their shoot density and biomass. The shoot density and biomass at Gabae population was $367.3 m^{-2}\;and\;725.7g$ dry wt $m^P{-2}$ respectively, while those at Jukrim population were $112.5m^{-2}\;and\;392.0g\;dry\;wt\;m^{-2}$, respectively.

Application Study of Dry-ice Pellet Cleaning for Removing Oil Paint and Lacquer of Outdoor Metal Artifacts (Dry-ice Pellet Cleaning 적용 옥외 금속문화재 표면 페인트 및 유성물질 제거방법 비교 연구)

  • Lee, Jee-Eun;Cho, Nam-Chul;Lee, Jong-Myoung;Yu, Jae-Eun
    • Journal of Conservation Science
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    • v.28 no.3
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    • pp.217-228
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    • 2012
  • Damage of cultural properties happens variously. Particularly, the surface damage of metal cultural properties exposed outdoors lowers historical and art historical value of artifact by artificial damage such as paint, scribbling containing oily material and so on. Therefore, this study compared dry-ice pellet cleaning with poultice when clearing paint and oily material environment-friendly, harmlessly to humans and without damage of artifact. As the result of experiment, when clearing those (paint and oily material) by poultice, oily paint was cleared, but there were spots of metal surface. Also, Lacquer spray wasn't well cleared, and resin came off the surface of artifact. When clearing those by dry-ice pellet cleaning, oil paint was cleanly cleared without surface damage of artifact according to Stereoscopic microscope observation, color-measurement, FT-IR, SEM analysis. Also, lacquer spray seemed to be cleared with the naked eye, but there were minute particles on surface according to the result of SEM observation. Consequently, we could confirm possibility of dry-ice pellet cleaning substitution.

Effects of Panax Notoginseng Flos extract on Anti-oxidative, Anti-inflammatory and Acne skin (삼칠화 에탄올 추출물의 항산화, 항염증 활성 및 여드름 피부에 미치는 영향)

  • Kim, Song-Yi;Li, Shun-Hua
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.1
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    • pp.43-54
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    • 2018
  • The purpose of this study is to observe how Panax Notoginseng Flos Ethanol Extract has anti-oxidant activity, cytotoxicity for skin cells, and anti-inflammatory effects and Cosmetics Panax Notoginseng Flos Ethanol Extract was applied to acne skin to analyze moisture, erythema, pores, and blackberries to identify potential as cosmetics material .According to the analysis, Panax Notoginseng Flos Ethanol Extract was found to have high contents of polyphenols and flavonoids and excellent DPPH radical scavenging activity. The extract had no significant cytotoxicity for Raw 264.7 cell and significantly inhibited the creation of NO induced LPS in Raw 264.7 cell so as to present anti-inflammatory effect. After applying an Panax Notoginseng Flos Ethanol Extract of 3% skin toner and spot Solutions to the skin of the skin of the acne for 3 weeks, Increased skin moisture, erythema, pores, black head count were found to be statistically signigicant, and reduced red spots were found in skin condition questions following management. Given the results, it is considered that Panax Notoginseng Flos Ethanol Extract is applicable as the ingredient of a functional cosmetic product that has low cytotoxicity for skin cells, high anti-oxidant activity, anti-inflammatory effect, and acne improvement effect.

Disease Severity of Angular Leaf Spot Disease by Different Inoculation Method and Eco-Friendly Control Efficacy in Strawberry. (접종방법에 따른 딸기모무늬병원균의 발병도 검증 및 친환경 방제 효과)

  • Kim, Da-Ran;Gang, Gun-hye;Cho, Hyun-ji;Yoon, Hae-Suk;Myoung, In Sik;kwak, Youn-Sig
    • The Korean Journal of Pesticide Science
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    • v.20 no.1
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    • pp.35-40
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    • 2016
  • Recently, bacterial angular leaf spot disease, caused by Xanthomonas fragariae, causes severe damage in strawberry production and its' export to other countries, since the pathogen has been classified as an A2 quarantine pathogen. Typical the Angular Leaf Spot (ALS) disease represent that water-soaked angular spots symptoms, bacteria ooze exudate under relatively high humidity condition and later the spot become reddish brown on the leaf surface. The pathogen disseminated by irrigation water, infected mother plant and farmer's hand. In this study, we reported that rubbing inoculation method showed more effective in the pathogen dissemination than infiltration with needles, regardless the strawberry cultivars. Additionally, Dichloroisocyanurate (NaDCC) treatment in commercial strawberry fields provided reliable efficiency to reduce the bacterial angular leaf spot disease incidency and severity.

Spatial Analysis of Colorectal Cancer Cases in Kuala Lumpur

  • Shah, Shamsul Azhar;Neoh, Hui-Min;Syed Abdul Rahim, Syed Sharizman;Azhar, Zahir Izuan;Hassan, Mohd Rohaizat;Safian, Nazarudin;Jamal, Rahman
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.3
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    • pp.1149-1154
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    • 2014
  • Background: In Malaysia, data from the Malaysian Health Ministry showed colorectal cancer (CRC) to be the second most common type of cancer in 2007-2009, after breast cancer. The same was apparent after looking at males and females cases separately. In the present study, the Geographic Information System (GIS) was employed to describe the distribution of CRC cases in Kuala Lumpur (KL), Malaysia, according to socio-demographic factors (age, gender, ethnicity and district). Materials and Methods: This retrospective review concerned data for patients diagnosed with colorectal cancer in the years 1995 to 2011 collected from the Wilayah Persekutuan Health Office, taken from the cancer notification form (NCR-2), and patient medical records from the Surgical Department, Universiti Kebangsaan Malaysia Medical Centre (UKMMC). A total of 146 cases were analyzed. All the data collected were analysed using ArcGIS version 10.0 and SPSS version 19.0. Results: Patients aged 60 to 69 years accounted for the highest proportion of cases (34.2%) and males slightly predominated 76 (52.1%), Chinese had the highest number of registered cases at 108 (74.0%) and staging revealed most cases in the 3rd and 4th stages. Kernel density analysis showed more cases are concentrated up in the northern area of Petaling and Kuala Lumpur subdistricts. Spatial global pattern analysis by average nearest neighbour resulted in nearest neighbour ratio of 0.75, with Z-score of -5.59, p value of <0.01 and the z-score of -5.59. Spatial autocorrelation (Moran's I) showed clustering significant with p<0.01, Z score 3.14 and Moran's Index of 0.007. When mapping clusters with hotspot analysis (Getis-Ord Gi), hot and cold spots were identified. Hot spot areas fell on the northeast side of KL. Conclusions: This study demonstrated significant spatial patterns of cancer incidence in KL. Knowledge about these spatial patterns can provide useful information to policymakers in the planning of screening of CRC in the targeted population and improvement of healthcare facilities to provide better treatment for CRC patients.

Nondestructive investigation of clay wall structure containing traditional mural paintings. - The clay walls having mural paintings housed in the protective building in Muwisa Temple, Kangjin, Jeollanamde Province - (전통 벽화의 토벽체 비파괴진단 조사연구 - 강진 무위사 벽화보존각내 벽화를 중심으로 -)

  • Chae, Sang-Jeong;Yang, Hee-Jae;Han, Kyeong-Soon
    • Journal of Conservation Science
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    • v.18 s.18
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    • pp.51-62
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    • 2006
  • This study, in order to do a nondestructive research on the mural walls kept in the protective house in Muwisa Temple, Kangjin, took four examinations; particle size analysis, XRD analysis, ultrasonic investigation, and thermo-infrared investigation. Component ratio of mural wall varied; clay of wall bodies consisted of gravel of 1.78 g, sand of 5.39 g, silt of 4.91 g and clay of 6.26 g. Ultrasonic velocity and one-axis compression strength tests done with eight mural-painted walls yield results as follows; the value of ultrasonic velocity ranged between 71.63 and 3610.11 m/s with the average of 417.44 m/s and on-axis compression strength ranged between 70.34 and $533.28kg/cm^2$ with the average of $83.23kg/cm^2$. The value increased in the order of Bosaldo(No.6)

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Analysis of Poly(3-Hydroxybutyrate) Granule-Associated Proteome in Recombinant Escherichia coli

  • Han Mee-Jung;Park Si-Jae;Lee Jeong-Wook;Min Byoung-Hoon;Lee Sang-Yup;Kim Soo-Jin;Yoo Jong-Shin
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.901-910
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    • 2006
  • Poly(3-hydroxybutyrate) [P(3HB)] is a microbial polyester intracellularly accumulated as distinct granules in numerous microorganisms as an energy and carbon storage material. Recombinant Escherichia coli harboring the heterologous P(3HB) biosynthesis genes accumulates large amounts of P(3HB) granules, yet the granule-associated proteins have not been identified. Therefore, this study reports on an analysis of the P(3HB) granule-associated proteome in recombinant E. coli. Fiye proteins out of 7 spots identified were found to be involved in functions of translation, heat-stress responses, and P(3HB) biosynthesis. Two of the major granule-associated proteins, IbpA/B, which are already known to bind to recombinant proteins forming inclusion bodies in E. coli, were further analyzed. Immunoblotting and immunoelectron microscopic studies with IbpA/B antibodies clearly demonstrated the binding and localization of IbpA/B to P(3HB) granules. IbpA/B seemed to play an important role in recombinant E. coli producing P(3HB) by stabilizing the interface between the hydrophobic P(3HB) granules and the hydrophilic cytoplasm. Thus, IbpA/B were found to act like phasins in recombinant E. coli, as they are the major proteins bound to the P(3HB) granules, affect the morphology of the granules, and reduce the amount of cytosolic proteins bound to the P(3HB) granules.

An Analysis on the Landscape Structure of Anuisamdong-Dongcheon in the 19th Century (19세기 안의삼동(安義三洞) 동천(洞天)의 경관구조 해석)

  • Kim, Dong-Hyun;Lee, Won-Ho
    • The Journal of the Korea Contents Association
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    • v.16 no.11
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    • pp.742-753
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    • 2016
  • This study aims to reproduce of Anuisamdong's landscape structure and improve the value of Scenic site. It was extract main spot from Geography book published by government in the 19th Century. And analyzed landscape architecture of Dongcheon. Extract main spot from Geography book published by government. Consequently, Hwarim-dong main spot 6 places, Simjin-dong main spot 9 places, and Wonhak-dong main spot 25 places were identified. Hwarim-dong's main spot was divided into 3 places of pavilion and scenery, respectively. Simjin-Dong's main spots was all that natural elements as a scenery. Wonhak-dong's main spot was the most elements of the Anuisamdong in geography book published by government. Hwarim-dong was pavilion-based scenery area complex, Simjin-dong was continues scenery spot along the valley, and Wonhak-dong was hybrid distribution that landscape structure. Each dongcheon in Anuisamdong was created a separate area through isolated geographical, visually for structure of collectively means. Also, a narrow entrance and enclosure topography are areas which arouse atmosphere of paradise. In addition, Aunuisamdong's landscape structure, which was previously divided into individual Dongcheon, was expanded into a single integrated landscape structure of Dongcheon. This is different from the general landscape development of common Dongcheon with the same starting and ending points and sightseeing routes.