• Journal of Acupuncture Research
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• v.23 no.3
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• pp.177-190
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• 2006

Objectives : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cell lines. Oligonucleotide microarray and proteomic approaches were employed to screen the differential expression genes. Methods : GRR~HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of GRR-HAS (0.1, 0.5, 1.5, 10, $20mg/m{\ell}$) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with $1.5mg/m{\ell}$ of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.). For proteomic analysis, total protein was analyzed by 2D gel electrophoresis and Q-TOF mass spectrometer. Results : It has no cytotoxic effects on both HepG2 cells in all concentrations(0.1, 0.5, 1.5, 10,$20mg/m{\ell}$). In oligonucleotide microarray assay, the number of more than twofold differentially regulated known genes was 320 with 6 up-regulated and 314 down-regulated genes in HepG2 cells. In proteomic analysis, three spots were identified by 2D-gel electrophoresis and Q-TOF analysis. One down -regulated protein was protein disulfide isomerase and up-regulated proteins were fatty acid binding protein 1 and 14-3-3 gan1lTIa protein by $1.5mg/m{\ell}$ of CRR-HAS. Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.5
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• pp.752-757
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• 2003

This study was carried out in order to develop the method for isolation of red ginseng acidic polysaccharide (RGAP) haying immunomodulating antitumor activity from red ginseng by-product. The red ginseng by-product was obtained from red ginseng residues produced in processing of red ginseng ethanol extract. The yield of RGAP isolated by ultrafiltration was 20.9%. The active substance (GFP) was purified by DEAE-sepharose column chromatography RGAP induced nitric oxide (NO) exhibited tumoricidal activities against P8l5 (mastocytoma) tumor cells. Acid-hydrolyzed RGAP fragments were shown four to five spots. These sopts showed the same R$_{f}$ values with sugars designated as rhamnose, glucose, glactose and glucuronic acid. Some physico-chemical properties of RGAP were investigated. pH and dry reduction content at 105$^{\circ}C$ were 4.74 and 4.72%, respectively. Crude protein, ash and Pb contents were 3.30%, 4.74% and 2.30 ppm. These results suggest that we will be able to produce RGAP from red ginseng by-product by ultrafiltration in a large scale.e.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.1
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• pp.7-14
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• 1974

1. Stylochus ijimai spawns from May to October with peak spawning from July to September, P. obscurus spawns from June to October with peak spawning from July to September. 2. S. ijimai spawns approximately 96,000-132,000 eggs by one spawning. 1. Just after spawnings, the eggs of S. ijimai were $102\mu$ in diameter, and those of P. obscurus were $108\mu$. 4. S. ijimai and P. obscurus have simple eggs. S. ijimai develop indirectly having Gotte larvae, while P. obscurus develop directly. 5. These two species show very similar patterns of early developments from maturation division of eggs to the gastrula stage. 6. The zygots of S. ijimai reaches mesentoblast stage 48 hours after fertilization, and Gotte larvae hatch out 7 days after fertilization. The zygots of P. obscurus reaches mesentoblast stage 72 hours after fertilization, and juveniles hatch out 14 days after fertilization. 7. S. ijimai, have 14 days of planktonic larvae stages bearing strong phototacic behavior : P. obscurus have 7 days of planktonic life without phototacic behavior. 8. Newly hatched larvae of S. ijimai and P. obscurus are $138\mu\;and\;170\mu$ in length respectively. The early creeping forms of S. ijimai and P. obscurus are $152\mu\;and\;185\mu$ in length respectively. 9. In the early creeping stage S. ijimai are characterized by testing processes and flattening of the body. In the same stage P. obscurus lost 2 eye-spots in the cerebral area. 10. The early creeping larvae of these two species were found only in mud-flat substrates.

• Journal of the Korean Institute of Landscape Architecture
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• v.48 no.5
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• pp.67-79
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• 2020

Plans to mitigate overheating in urban areas requires the identification of the characteristics of the thermal environment of the city. The key information is the distribution of higher and lower temperatures (referred to as "hotspot" or "coldspot", respectively) in the city. This study aims to identify the areas within Jeonju City that are suffering from increasing land surface temperatures (LST) and the factors linked to such this phenomenon. To identify the hot and cold spots, Local Moran's I and Getis-Ord Gi^* were calculated for the LST based on 2017 images taken using the thermal band of the Landsat 8 satellite. Hotspot analysis revealed that hotspot regions, (the areas with a high concentration of Land Surface Temperature) are located in the old town area and in industrial districts. To figure out the factors linked to the hotspots, a correlation analysis, and a regression analysis taking into account environmental covariates including Normalized Difference Vegetation Index (NDVI) and land cover. The values of NDVI showed that it had the strongest effect on the lowering LSTs. The results of this study are expected to provide directions for urban thermal environment designing and policy development to mitigate the urban heat island effect in the future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.1
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• pp.143-153
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• 2008

Recently has there been a great increase of the interests in the excavation of governing institutions and their restoration. There also arise discussions on whether the municipal governments should appoint old governing institutions as the historical spots. In the course of the excavation and restoration of old governing institutions, their practical use becomes a matter of concern. So the reproduction of the past ritual ceremonies and events in the governing institutions attracts considerable attention. But the discovery and the analysis of historical materials are very insufficient to meet the recent increase of such demand. In that point of view, this study aims to analyze the ceremony of Kwanchalsa's(觀察使; a provincial governor) arrival at one's post and the use of governing institutions for it and to examine the Kwanchalsa's route of sulryeok(巡歷) and the ritual ceremonies held in the cities he visited. The Chungcheong-Gamsa(忠淸監司; the governor of Chungcheong-Do) in the 18th century is the subject of this analysis and three local officials' diaries - Chungcheong Gamyeong Ilgi ($\ulcorner$忠淸監營日記$\lrcorner$), Hoseo Gamyeong Ilgi ($\ulcorner$湖西監營日記$\lrcorner$), Geumyeong Ilgi($\ulcorner$錦營日記$\lrcorner$) - are closely investigated for that purpose.

• Korean Journal of Remote Sensing
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• v.36 no.2_1
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• pp.139-153
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• 2020

Using a computationalfluid dynamics(CFD) model, thisstudy evaluated the representativeness of an air quality monitoring system (AQMS) in an urban area and presented a methodology to determine the suitable AQMS locations for specific purposes. For this, we selected a 1.6 km × 1.6 km area around the Eunpyeong-gu AQMS (AQMS 111181) as a target area. We conducted simulationsfor two emission scenarios (scenario one: air pollutants transported from inflow boundaries, scenario two: air pollutants emitted from roads). Urban airflows were markedly influenced by mountainous terrain located in the northeast and southeast of the target area, and complicated airflow patterns occurred around the buildings. The distributions of air pollutants were dependent on the terrain (mountain) in scenario one, but the road location and building height in scenario 2. We evaluated whether the AQMS could represent the air quality in the target area based on the simulations for two scenarios. The concentrations simulated at the AQMS were similar in magnitude to the layer mean concentrations, which indicated good representativeness for the air quality in the target area. We also suggested which locations were suitable for different measurement purposes (hot spots, clean zones, average zones, shelter zones, equi-background zones).

• Journal of Mushroom
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• v.11 no.1
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• pp.32-35
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• 2013

The purpose of this study is to the Gyeongbuk area of mushroom exports by increasing exports strategy, and export support for establishing a policy analysis of the factors that you can take advantage of the basic information to get. Mushroom industry has made a rapid growth over the past 20 years in Korea. Gyeongbuk province mushroom exports account for about half of the country, enoki and total exports in 2009, and 81%, increased to 14% in 2010 and 2010, the maximum exports (9,415 tons) and the maximum exports (14,840 grand)was recorded. King oyster mushroom to represent the Netherlands in Europe, exports were higher exports to China is negligible. In the case of China in 2001, 5 spots automated mushroom factory, production, but only 8,000 tons in 2011, 652 mushroom factory, production of 1,100,000 tons very quickly grew.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.251-256
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• 2005

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching, We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.301-306
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• 2005

Conditions for efficient organogenesis and plant regeneration from Dianthus gratianopol suspension cultured cells were established. Shoot-forming calli of glossy surface, pale green and knobby type were selected from leaf explant-derived calli and were suspension-subcultured every week in CP liquid medium with 1.0 mg/L 2,4-D and 0.5 mg/L BAP. Combinations of 1.0 mg/L 2,4-D and 0.5 mg/L BAP, and 1.5 mg/L 2,4-D and 0.5 mg/L BAP were effective for the induction of regenerative callus from the suspension cultured cell clusters. Multiple shoot primordia were initiated from the green spots of these regenerative callus and formed shoots on MS medium with 1.0 mg/L TDZ and 0.5 mg/L PAA. Shoot regeneration frequency (calli regenerating at least one shoot) was about 87%. For plant regeneration, proliferated shoots were excised and transferred to MS medium with 0.1 mg/L NAA for root initiation after 9 weeks of culture. The regenerants were potted in soil and formed the flowering buds and petals. Also, adventitious shoots were formed from the excised green shoot primordia of regenerative callus and these shoots proliferated successfully and regenerated to whole plants.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.5
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• pp.426-431
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• 2007

When a high-energy ultrasound propagates through a solid body that contains a crack or a delamination, the two faces of the defect do not ordinarily vibrate in unison, and dissipative phenomena such as friction, rubbing and clapping between the faces will convert some of the vibrational energy to heat. By combining this heating effect with infrared imaging, one can detect a subsurface defect in material in real time. In this paper a realtime detection of the brazing defect of thin Inconel plates using the UIR (ultrasonic infrared imaging) technology is described. A low frequency (23 kHz) ultrasonic transducer was used to infuse the welded Inconel plates with a short pulse of sound for 280 ms. The ultrasonic source has a maximum power of 2 kW. The surface temperature of the area under inspection is imaged by an infrared camera that is coupled to a fast frame grabber in a computer. The hot spots, which are a small area around the bound between the two faces of the Inconel plates near the defective brazing point and heated up highly, are observed. And the weak thermal signal is observed at the defect position of brazed plate also. Using the image processing technology such as background subtraction average and image enhancement using histogram equalization, the position of defective brazing regions in the thin Inconel plates can be located certainly.