• Journal of Life Science
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• v.20 no.5
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• pp.789-793
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• 2010

Artificial chromosome manipulation and amplification of single-copy yeast artificial chromosome (YAC) are usually required in order to use YACs for applications such as physical mapping and functional analysis in eukaryotes. We designed and implemented a Simultaneous YAC Manipulation-Amplification (SYMA) system that combines the copy number amplification system of YAC with a convenient YAC manipulation system. To achieve the desired split and to amplify a YAC clone-harboring plant chromosome, a pBGTK plasmid containing a conditional centromere and thymidine kinase (TK) gene was constructed as a template to amplify the splitting fragment via PCR. By splitting, new 490-kb and 100-kb split YACs containing the elements for copy number amplification were simultaneously generated from a 590-kb YAC clone. The 100-kb split YAC was then successfully amplified 14.4-fold by adding 3 mg/ml sulfanilamide and $50\;{\mu}g/ml$ methotrexate (S3/M50) as inducing substances.

• Explosives and Blasting
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• v.28 no.2
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• pp.1-16
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• 2010

This study focuses on the phenomenon that the blast damaged zone developed on rock slope surfaces can be affected by joint characteristics rather than by explosive power when the pre-splitting is applied to excavate a jointed rock slope. The characteristics of rock joints on a slope were investigated and categorized them into 4 cases. Also an image processing system has been used for comparing the distribution pattern of rock blocks. From this investigation, it was found that the rock blocks bigger than 2,000 mm occupied 42% in the case of single joint set and it showed the well efficiency of pre-splitting blast. In cases of 2~3 parallel joint sets and 2~3 intersecting joint sets are developed on rock surfaces, the rock blocks in the range of 1,000~2,000 mm occupied 43.6% and 35.8%, respectively, and it showed that the efficiency of pre-splitting was decreased. When more than 3 joint sets are randomly developed, however, the rock blocks in the range of 250~500 mm occupied 35% and there was no block bigger than 1,000 mm. This denotes that the blasting with pre-splitting was not effective. The numerical analysis using PFC2D showed that the blast damaged zone in a rock mass could be directly influenced by the pre-splitting. It is, therefore, required to investigate the discontinuity pattern on rock surfaces in advance, when the pre-splitting method is applied to excavate jointed rock slopes and to apply a flexible blating design with a consideration of the joint characteristics.

• Journal of Families and Better Life
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• v.21 no.1
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• pp.61-72
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• 2003

This study examines the problematic fact that most housewives are excluded from receiving the benefits of the National Pension Plan in South Korea. The National Pension Plan assigns no value to housework or household production, which in turn discourages full-time housewives from participating voluntarily in the Plan. In this article, I propose to utilize Credit Splitting and Pension Sharing in order to take into account full-time housewives' economic contribution in the National Pension Plan. In this article, I also discuss the ranges and application methods of the Credit Splitting and Pension Sharing. For this study, I have analyzed the data of 11,967 unemployed married women living with spouses published in“Research Data on Everyday Life Time Usage”by the Korea National Statistical Office in 1999. The value of the full-time housewives' labor varies depending on the methods of estimation. However, all estimated values exceed the average value assigned to the housewives by the National Pension Corporation. It is clear that full-time housewives' unpaid labor contributes a great deal to the formation of household property and wealth, which is a valid reason for Pension Sharing and Credit Splitting. This article also provides logical factors to consider in the process of Pension Sharing and Credit Splitting, which can be used for developing computerized software to determine a full-time housewives' labor value at the time of divorce or for any other purpose.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.313-318
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• 2006

A two-stage process of nanofiltration and water-splitting electrodialysis was investigated for lactic acid recovery from fermentation broth. In this process, sodium lactate is isolated from fermentation broth in the first stage of nanofiltration by using an NTR-729HF membrane, and then is converted to lactic acid in the second stage by water-splitting electrodialysis. To determine the optimal operating conditions for nanofiltration, the effects of pressure, lactate concentration, pH, and known added impurities were studied. Lactate rejection was less than 5%, magnesium rejection approximated 45%, and calcium rejection was at 40%. In subsequent water-splitting electrodialysis, both the sodium lactate conversion to lactic acid and sodium hydroxide recovery, were about 95%, with a power requirement of $0.9{\sim}1.0\; kWh$ per kg of lactate.

• Explosives and Blasting
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• v.28 no.1
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• pp.63-70
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• 2010

This study is investigated the extent of the noise and ground vibration in an adjacent zone of a cattle pen and an antiquated housing structures for judgement of the spot applicability on the extents of the noise and ground vibration of the rock-splitting method by an oil pressure. It is studied by measuring and analysing in an adjacent position the extents of the noise and ground vibration according to the work process of the rock-splitting method, such as drilling, rock-splitting, arranging rock, loading and by being compared with the permitted level on the noise and ground vibration fixed at the spot. To the results, it is identified that the influence to the noise has to be considered, even if the rock-splitting method is applied as an excavation method to lower a ground vibration by the classification on blasting method of the ministry of land, transport and marine affairs.

• 한국전산유체공학회:학술대회논문집
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• 2005.10a
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• pp.117-124
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• 2005

Splitting algorithms of the incompressible Navier-Stokes equations using P1P1/P2P1 finite element formulation are newly proposed. P1P1 formulation allocates velocity and pressure at the same nodes, while P2P1 formulation allocates pressure only at the vertex nodes and velocity at both the vertex and mid nodes. For comparison of the elapsed time and accuracy of the two methods, they have been applied to the well-known benchmark problems. The three cases chosen are the two-dimensional steady and unsteady flows around a fixed cylinder, decaying vortex, and impinging slot jet. It is shown that the proposed P2P1 semi-splitting method performs better than the conventional P1P1 splitting method in terms of both accuracy and computation time.

• Computers and Concrete
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• v.3 no.6
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• pp.389-400
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• 2006

This paper presents the investigations towards developing a better understanding on the contribution of steel fibers on the tensile strength of high-performance fiber reinforced concrete (HPFRC). An extensive experimentation was carried out with w/cm ratios ranging from 0.25 to 0.40 and fiber content ranging from zero to 1.5 percent with an aspect ratio of 80. For 32 concrete mixes, flexural and splitting tensile strengths were determined at 28 days. The influence of fiber content in terms of fiber reinforcing index on the flexural and splitting tensile strengths of HPFRC is presented. Based on the test results, mathematical models were developed using statistical methods to predict 28-day flexural and splitting tensile strengths of HPFRC for a wide range of w/cm ratios. The expressions, being developed with strength ratios and not with absolute values of strengths and are applicable to wide range of w/cm ratio and different sizes/shapes of specimens. Relationship between flexural and splitting tensile strengths has been developed using regression analysis and absolute variation of strength values obtained was within 3.85 percent. To examine the validity of the proposed model, the experimental results of previous researchers were compared with the values predicted by the model.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.21 no.1
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• pp.1-16
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• 2017

The Allen-Cahn equation is solved numerically by operator splitting Fourier spectral methods. The basic idea of the operator splitting method is to decompose the original problem into sub-equations and compose the approximate solution of the original equation using the solutions of the subproblems. The purpose of this paper is to characterize higher order operator splitting schemes and propose several higher order methods. Unlike the first and the second order methods, each of the heat and the free-energy evolution operators has at least one backward evaluation in higher order methods. We investigate the effect of negative time steps on a general form of third order schemes and suggest three third order methods for better stability and accuracy. Two fourth order methods are also presented. The traveling wave solution and a spinodal decomposition problem are used to demonstrate numerical properties and the order of convergence of the proposed methods.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.3
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• pp.391-398
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• 2012

Detecting races is important for debugging shared-memory parallel programs, because the races result in unintended non-deterministic executions of the programs. Previous on-the-fly techniques to detect races in parallel programs with general inter-thread coordination show serious space overhead which depends on the maximum parallelism of the program. Therefore, this paper presents a loop splitting technique for on-the-fly race detection of parallel programs which is more efficient in space complexity than previous techniques. This loop splitting technique is the debugged program which preserves semantics of the original program. Monitering loop splitting program in on-the-fly can detect first races.

• Journal of Energy Engineering
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• v.15 no.2 s.46
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• pp.96-106
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• 2006

Among several different hydrogen production technologies, solar hydrogen system for water splitting is the only clean and sustainable energy supplier. Hydrogen production by water-splitting utilizing solar energy has attracted considerable interest since the pioneering work of Honda and Fujishima in 1979, who discovered that water can be photo-electrochemically decomposed into hydrogen and oxygen using a semiconductor ($TiO_2$) electrode under UV irradiation. Most efforts to utilize solar ray lead to explore visible responding photocatalysts, PEC cells and other fusion technology like bio-photocatalytic conversion. In this paper, photon utilization technologies for water splitting have been briefly reviewed except solar thermal utilization technology.