• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.479-485
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• 2002

This study was conducted in order to elucidate the effects of nitrogen level and split application method, and shading treatment during reproductive stage on sink formation. Japonica variety Choocheongbyeo and Hwaseongbyeo and indica$\times$japonica cross type variety Nampoongbyeo were used. Five levels (6 to 30 kg/10a at 6 kg/10a interval) of nitrogen fertilization, and two split application methods (50-25-25% and 30-30-40% as basaltillering-panicle fertilizer) for each nitrogen treatment were applied. In addition shading treatments (shading rate, 65%) were performed for N 12 kg/10a and 24 kg/10a plot. Shading were applied for 30 days from panicle initiation to heading, 15 days from panicle initiation and 15days before heading. Panicle per square meter, and primary rachis branches per panicle and differentiated number of secondary branch per panicle increased according as applied nitrogen amount increased up to 18 to 24 kg/10a, and there was no significant difference between two nitrogen application methods. Primary rachis branch and secondary branch per square meter also increased according as the amount of applied nitrogen increase up to 18 to 24 kg/10a, and there was no significant difference between nitrogen application methods. Panicle per square meter and primary rachis branch per panicle were significantly decreased due to shading treatments only in Choochengbyeo. In all varieties, shading reduced secondary rachis branch per panicle significantly and the reduction was greatest in 30 days shading during reproductive stage. Spikelets per square meter increased according as the amount of applied nitrogen increases up to 18 to 24kg/10a, but showed no move increase above this nitrogen application level. Significant difference was not shown between nitrogen split methods. Spikelets per square meter also decreased significantly due to shading treatment during reproductive stage, showing the greatest reduction by 30 days shading during reproductive stage, and the least by 15 days shading during booting stage. The variation of spikelets per square meter was influenced greatest by the variation of panicles per square meter and spikelets per secondary rachis branch.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2001.10a
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• pp.37-43
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• 2001

1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7~8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr. Hashiyada(2001), 296 pairs of split-half embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs. Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1988, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a glaf of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us as effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle. 6. Farm animal cloning is one of the most dreamful technologies of 21th century. It is necessary to develop this technology more efficient and stable as realistic technology of the farm animal production. We are making researches related to the best condition of donor cells for high productivity of cloning, genetic analysis of cloned animals, growth and performance abilities of clone cattle and pathological and genetical analysis of high rates of abortion and stillbirth of clone calves (about 30% of periparutum mortality). 7. It is requested in the report of Ministry of Health, labor and Welfare to make clear that carbon-copy cattle(somatic cell clone cattle) are safe and heathy for a commercial market since the somatic cell cloning is a completely new technology. Fattened beef steers (well-proved normal growth) and milking cows(shown a good fertility) are now provided for the assessment of food safety.

• Applied Biological Chemistry
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• v.25 no.4
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• pp.232-238
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• 1982

From ^{15}N$ labelled nitrogen experiments on 13 fields use efficiency by difference method was higher than that by labelling method in 80% of fields tested indicating augmentation of soil nitrogen uptake by fertilizer nitrogen. Both methods showed very similar trend among fields. Sulfur coated urea(SCU) and point application increased fertilization efficiency(yield increment per fertilizer nitrogen applied, Fe) to 23 from 15 of split application through the increase of fertilizer use efficiency from 29(Eu) to 50 but tended to decrease efficiency of absorbed fertilizer nitrogen(yield increment per nitrogen derived from fertilizer, Ef) from 50. to 46 High yielding capacity of Tongil line appears to be attributed to the higher Ef, translocation efficiency and soil nitrogen preference index(soil nitrogen increment in plant per the increment of fertilizer nitrogen in plant, PI). This studies confirmed that yield under fertilizer application system depends on Fe which is the multiplication of Eu and Ef and that the improvement of fertilizer management(form, application method and time) increases principally Eu, the limit of which is controlled by Ef that is attributed mainly to varietal characteristics.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.206-206
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• 2017

Sorghum is a crop with a various plant height depending on the planting density. If the height exceeds 1.8m, which is the harvestable height of the combine, loss is caused by clogging of the installation, entrance of the threshing section and the threshing section. The purpose of this study is to set the planting distance and number of plants per hill suitable for combine harvesting as the plant length does not exceed 1.8m. The experimental variety was Nampungchal. The experiment design was a split-plot design with three replications. The treatments were as follow: Main-plot were 1 and 2 plants as number of plants per hill and sub-plots were $60{\times}20cm$ (practice), $70{\times}15$, 20, 25, 30 cm as planting distance. The amount of nitrogen, phosphate and potassium fertilization were 100, 70, $80kg\;ha^{-1}$. Data were collected: (1) grain yield: weight of grain in $kg\;ha^{-1}$, (2) 1000 grain weight: average weight of 1000 grain, (3) plant height: distance from soil to top of panicle, (4) ear length: distance from top of stem to top of ear in cm, (5) stem diameter: diameter of second internode, (6) tiller number per hill. Analyses of variance were performed using R version 3.3.1(https://www. r- project. org). The Duncan's multiple range test(DMR) was used to separate treatment means at P < 0.05. As number of plants per hill increased, plant height and yield increased and tiller number decreased. As planting distance increased, plant height and yield decreased and tiller number increased. At 1 plant per hill, the plant height did not exceed 1.8m at all planting distance. At 2 plants per hill, the plant height did not exceed 1.8m from the planting distance of $70{\times}25cm$. At 1 plant per hill, the tiller number increased to 0.23, 0.27, 0.60 and 0.70 as the planting distance increased to $70{\times}15$, 20, 25 and 30 cm, respectively. At 2 plants per hill, the tiller number increased to 0.03, 0.03, 0.14 and 0.40 as the planting distance increased to $70{\times}15$, 20, 25 and 30 cm, respectively. At 1 plant per hill, the yield decreased to 6030, 4280, 3400 and $3230kg\;ha^{-1}$ as the planting distance increased. At 2 plant per hill, the yield decreased to 7850, 5770, 5720 and $4960kg\;ha^{-1}$ as the planting distance increased. We recommend that the optimum number of plants per hill and planting distance is 2 and $70{\times}25cm$ suitable for combine harvesting.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.207-207
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• 2017

The proso millet is vulnerable to lodging due to high plant height and shallow root. A lodging results in a hard mechanical harvesting and yield loss. One of solutions on this problem is inhibition of internode elongation. The objective of this study was to set up use time and dose of prohexadium-calcium, is growth inhibitor. The experimental variety was Ibaekchal. The experiment design was a split-plot design with three replications. The treatments were as follow: Main-plots were 25 and 35 day after sowing(DAS) as use time and sub-plots were 0%, 50%, 100%(diluted solution of 1000 times, $1000{\ell}\;ha^{-1}$), 150% as dose. The amount of nitrogen, phosphate and potassium fertilization were 90, 70, $80kg\;ha^{-1}$, respectively. The size of high ridge and plant spacing were $90{\times}30cm$ and $60{\times}15cm$, respectively. Proso millet was sown on June 9, 2016 by hands and was adjusted at 2 plant per hill. The growth survey of vegetative growth stage was conducted at 1 day before treatment and with one week interval after treatment. Data were collected: (1) grain yield: weight of grain in $kg\;ha^{-1}$, (2) 1000 grain weight: average weight of 1000 grain, (3) plant height: distance from soil to top of panicle or leaf in cm, (4) ear length: distance from top of stem to top of ear in cm, (5) stem diameter: diameter of second internode (6) degree of lodging: percentage of lodging area, etc. Analyses of variance were performed using R version 3.3.1(https://www. r- project. org). The Duncan's multiple range test(DMR) was used to separate treatment means at P < 0.05. There was a significant difference in plant height and number of stem among the use time and dose of prohexadium-calcium during vegetative growth stage. At 25 DAS, the difference with no treatment increased until 25 day after treatment and decreased since then. The difference in number of stem increased until 18 day and decreased since 25 day. At 35 DAS, the difference with no treatment in plant height and number of stem increased until 22 day after treatment and decreased since then. We assumed that the effect of prohexadium-calcium was inhibition of internode elongation and promotion of tillering, continued untel 25day after treatment. At 25 DAS, the degree of lodging deceased to 100%, 30%, 10% and 0% as dose increased. At 35 DAS, the degree of lodging decreased to 100%, 20%, 0% and 0% as dose increased. At 25 DAS, the yield was 2910, 2710, 3190, $2310kg\;ha^{-1}$ among dose. At 35 DAS, the yield was 2750, 2630, 2220, $2050kg\;ha^{-1}$. We recommend that the optimum use time and dose of prohexadium-calcium for proso millet is 1000 times diluted solution of $1000{\ell}$ per ha at 25 day after sowing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s02
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• pp.66-80
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• 1989

Salt injury in rice is caused mainly by the salinity in soil and in the irrigated water, and occasionaly by salinity delivered through typhoon from the sea. The salt concentration of rice plants increased with higher salinity in the soil of the rice growing. The climatic conditions, high temperature and solar radiation and dry conditions promote the salt absorption of rice plant in saline soil. The higher salt accumulation in the rice plant generally reduces the root activity and inhibits the absorption of minerals of rice plant, resulting the reduction of photosynthesis. The salt damages of rice plant, however, are different from different growth stage of rice plants as follows: 1. Germination of rice seed was slightly delayed up to 1.0％ of salt concentration and remarkably at 1. 5％, but none of rice seeds were germinated at 2.5％. This may be due to the delayed water uptake of rice seeds and the inhibition of enzyme activity, 2. It was enable to establish rice seedlings at seed bed by 0.2％ of salt concentration with some reduction of leaf elongation. The increasing of 0.3％ salt concentration caused to the seedling death with varietal differences, but most of seedlings were death at 0.4％ with no varietal differences. 3. Seedlings grown at the nursery over 0.1％ salt, gradually reduced in rooting activity after transplanting according to increasing the salt concentration from 0.1％ up to 0.3％ of paddy field. However, the seedlings grown in normal seed bed showed no difference in rooting between varieties up to 0.1％ but significantly different at 0.3％ between varieties, but greatly reduced at 0.5％ and died at last in paddy after transplanting. 4. At panicle initiation stage, rice plant delayed in heading by salt damage, at meiotic stage reduced in grains and its filling rate due to inhibition of glume and pollen developing, and salt damage at heading stage and till 3 weeks after heading caused to reduction of fertilization and ripening rate. In viewpoint of agricultural policy the overcoming strategy for salt injury is to secure sufficient water source. Irrigation and drainage systems as well as underground drainage is necessary to desalinize more effectively. This must be the most effective and positive way except cost. By cultural practice, growing the salt tolerant variety with high population could increase yield. The intermittent irrigation and fresh water flooding especially at transplanting and from panicle initiation to heading stage, the most sensitive to salt injury, is important to reduce the salt content in saline soil. During the off-cropping season, plough and rotavation with flooding followed by drainage, or submersion and drainage with groove could improve the desalinization. Increase of nitrogen fertilizer with more split application, and soil improvement by lime, organic matter and forign soil addition, could increase the rice yield. Shift of trans-planting is one of the way to escape from the salt injury.

• Proceedings of the KSAR Conference
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• 2001.10a
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• pp.37-43
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• 2001

1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes Collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7 -8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr.Hashiyada (2001), 296 pairs of split-half-embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs.Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1998, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a half of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us an effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle.

• Journal of the Korea Organic Resources Recycling Association
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• v.29 no.2
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• pp.15-23
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• 2021

This study was conducted to evaluate the field application of the developed recommendation system in organic rice (Oriza sativa L.) paddy and to investigate the mineral nitrogen content in soil and rice productivity. According to the developed system, hairy vetch (HV), rye+rapeseed oil cake (R+OC), rapeseed oil cake (OC) for only basal fertilization (OC-B), OC for split application (OC-S), pig manure compost (PMC), and chemical fertilizer (CHM) were applied to paddy soil at the rate of 107~133 kg N/ha. Results were followed, unhulled rice yield of OC-S (111%), OC-B (110), R+OC (106), HV (101), and PMC (96) were no significantly different with CHM (100). Also there was positive correlation (R²=0.803^*) between unhulled rice yield and cumulative inorganic N in soil. For nitrogen use efficiency of rice, OC-B, OC-S, and R+OC were not significantly different with CHM. In conclusions, the developed organic materials use recommendation system was effective for organic rice productivity. It could be useful for organic farmer to apply the organic materials use recommendation system for rice.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.100-105
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• 1985

To find out the optimum level of nitrogen fertilization and better distribution of application time for seed production of Festuca arundianacea S. (var. Alta), this filed experiment was performed at Livestock Experiment Station in Suweon, during 1979 to 1981. The treatments used in this study were three nitrogen fertilizing levels (120, 180 and 240kg/ha) and four different methods of nitrogen distribution (i. single application of whole amount in early Spring, ii. 50 percent each in Autumn and early Spring, iii. 50 percent each in early Spring and at begin of heading stage and iv. 50 percent in Autumn and 25 percent in early Spring and at begin of heading stage respectively). The experiment was arranged as a split-plot design with 4 replications and performed at the experimental field of the Livestock Experiment Station in Suweon, during 1979 to 1981. The results obtained are summarized as follows: 1. Date of heading stage of Festuca arundinacea was about May 21 and that of flowering stage was June 1 to 3. The optimum stage for the seed harvesting of Festuca arundinacea (var. Alta) was June 25 about 22 to 23 days after full flowering stage. Average plant height was about 127cm and the panicle length, 24cm. 2. 1000 grain weight was 2.72g and the number of panicles were 85 to 107 per square meter. 3. The mean seed wield for two years was 678.8kg/ha with the average of whole treatments and 781.9kg/ha with the best treatment (50 percent in Autumn and 25 percent in early Spring and at begin of heading stage with 240kg/ha respectively). 4. The average germination rate of harvesting seeds was 87.0 percent and it was increasing trend according to frequent application of fertilizer. 5. The average DM yield of aftermath seed harvesting was 6155kg/ha with two cut, and it was the largest DM yield from the higher nitrogen level and also from the single application in early Spring.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.312-318
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• 2010

This study was carried out to develop and test a prototype program that recommends the nitrogen topdressing rate using the color digital camera image taken from rice field at panicle initiation stage (PIS). This program comprises four models to estimate shoot N content (PNup) by color digital image analysis, shoot N accumulation from PIS to maturity (PHNup), yield, and protein content of rice. The models were formulated using data set from N rate experiments in 2008. PNup was found to be estimated by non-linear regression model using canopy cover and normalized green values calculated from color digital image analysis as predictor variables. PHNup could be predicted by quadratic regression model from PNup and N fertilization rate at panicle initiation stage with $R^2$ of 0.923. Yield and protein content of rice could also be predicted by quadratic regression models using PNup and PHNup as predictor variables with $R^2$ of 0.859 and 0.804, respectively. The performance of the program integrating the above models to recommend N topdressing rate at PIS was field-tested in 2009. N topdressing rate prescribed for the target protein content of 6.0% by the program were lower by about 30% compared to the fixed rate of 30% that is recommended conventionally as the split application rate of N fertilizer at PIS, while rice yield in the plots top-dressed with the prescribed N rate were not different from those of the plots top-dressed with the fixed N rates of 30% and showed a little lower or similar protein content of rice as well. And coefficients of variation in rice yield and quality parameters were reduced substantially by the prescribed N topdressing. These results indicate that the N rate recommendation using the analysis of color digital camera image is promising to be applied for precise management of N fertilization. However, for the universal and practical application the component models of the program are needed to be improved so as to be applicable to the diverse edaphic and climatic condition.