• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2006.11a
• /
• pp.128-135
• /
• 2006

This paper presents a finite element method to analyze the free vibration of a flexible HDD composed of the spinning disk-spindle system with fluid dynamic bearings(FDBs), the head-suspension-actuator with pivot bearings, and the base plate with complicated geometry. Experimental modal testing shows that the proposed method well predicts tue vibration characteristics of a HDD. This research also shows that even the vibration motion of the spinning disk corresponding to half-speed whirl and the pure disk mode are transferred to a head-suspension-actuator and base plate through the air bearing and the pivot bearing consecutively. The proposed method can be effectively extended to investigate the forced vibration of a HDD and to design a robust HDD against shock.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.17 no.1 s.118
• /
• pp.24-32
• /
• 2007

This paper presents a finite element method to analyze the free vibration of a flexible HDD composed of the spinning disk-spindle system with fluid dynamic bearings(FDBs), the head-suspension-actuator with pivot bearings, and the base plate with complicated geometry. Experimental modal testing shows that the proposed method well predicts the vibration characteristics of a HDD. This research also shows that even the vibration motion of the spinning disk corresponding to half-speed whirl and the pure disk mode are transferred to a head-suspension-actuator and base plate through the air bearing and the pivot bearing consecutively. The proposed method can be effectively extended to investigate the forced vibration of a HDD and to design a robust HDD against shock.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.15 no.3 s.96
• /
• pp.251-258
• /
• 2005

The free vibration of a spinning flexible disk-spindle system supported by hydro dynamic bearings (HDB) in an HDD is analyzed by FEM. The spinning flexible disk is described using Kirchhoff plate theory and von Karman non-linear strain, and its rigid body motion is also considered. It is discretized by annular sector element. The rotating spindle which includes the clamp, hub, permanent magnet and yoke, is modeled by Timoshenko beam including the gyroscopic effect. The flexible supporting structure with a complex shape which includes stator core, housing, base plate, sleeve and thrust pad is modeled by using a 4-node tetrahedron element with rotational degrees of freedom to satisfy the geometric compatibility. The dynamic coefficients of HDB are calculated from the HDB analysis program, which solves the perturbed Reynolds equation using FEM. Introducing the virtual nodes and the rigid link constraints defined in the center of HDB, beam elements of the shaft are connected to the solid elements of the sleeve and thrust pad through the spring and damper element. The global matrix equation obtained by assembling the finite element equations of each substructure is transformed to the state-space matrix-vector equation, and the associated eigen value problem is solved by using the restarted Arnoldi iteration method. The validity of this research is verified by comparing the numerical results of the natural frequencies with the experimental ones. Also the effect of supporting structures to the natural modes of the total HDD system is rigorously analyzed.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2003.11a
• /
• pp.572-578
• /
• 2003

The free vibration of a spinning flexible disk-spindle system supported by hydro dynamic bearings in a HDD is analyzed by FEM. The spinning flexible disk is described using Kirchhoff plate theory and von Karman non-linear strain, and its rigid body motion is also considered. It is discretized by annular sector element. The rotating spindle which includes the clamp, hub, permanent magnet and yoke, is modeled by Timoshenko beam including the gyroscopic effect. The flexible supporting structure with a complex shape which includes stator core, housing, base plate, sleeve and thrust pad is modeled by using a 4-node tetrahedron element with rotational degrees of freedom to satisfy the geometric compatibility. The dynamic coefficients of HDB are calculated from the HDB analysis program, which solves the perturbed Raynolds equation using FEM. Introducing the virtual nodes and the rigid link constraints defined in the center of HDB, beam elements of the shaft are connected to the solid elements of the sleeve and thrust pad through the spring and damper element. The global matrix equation obtained by assembling the finite element equations of each substructure is transformed to the state-space matrix-vector equation, and the associated eigenvalue problem is solved by using the restarted Arnoldi iteration method. The validity of this research is verified by comparing the numerical results of the natural frequencies with the experimental ones. Also the effect of supporting structures to the natural modes of the total HDD system is rigorously analyzed.

• Journal of Ginseng Research
• /
• v.46 no.3
• /
• pp.481-488
• /
• 2022

Background: Although the tumor-suppressive effects of ginsenosides in cell cycle have been well established, their pharmacological properties in mitosis have not been clarified yet. The chromosomal instability resulting from dysregulated mitotic processes is usually increased in cancer. In this study, we aimed to investigate the anticancer effects of ginsenoside Rg1 on mitotic progression in cancer. Materials and methods: Cancer cells were treated with ginsenoside Rg1 and their morphology and intensity of different protein were analyzed using immunofluorescence microscopy. The level of proteins in chromosomes was compared through chromosomal fractionation and Western blot analyses. The location and intensity of proteins in the chromosome were confirmed through immunostaining of mitotic chromosome after spreading. The colony formation assays were conducted using various cancer cell lines. Results: Ginsenoside Rg1 reduced cancer cell proliferation in some cancers through inducing mitotic arrest. Mechanistically, it inhibits the phosphorylation of histone H3 Thr3 (H3T3ph) mediated by Haspin kinase and concomitant recruitment of chromosomal passenger complex (CPC) to the centromere. Depletion of Aurora B at the centromere led to abnormal centromere integrity and spindle dynamics, thereby causing mitotic defects, such as increase in the width of the metaphase plate and spindle instability, resulting in delayed mitotic progression and cancer cell proliferation. Conclusion: Ginsenoside Rg1 reduces the level of Aurora B at the centromere via perturbing Haspin kinase activity and concurrent H3T3ph. Therefore, ginsenoside Rg1 suppresses cancer cell proliferation through impeding mitotic processes, such as chromosome alignment and spindle dynamics, upon depletion of Aurora B from the centromere.

• Development and Reproduction
• /
• v.15 no.3
• /
• pp.231-237
• /
• 2011

The objective of this study was to elucidate the dynamics of microtubules in post-ovulatory aging in vivo and in vitro of mouse oocytes. The fresh ovulated oocytes were obtained from oviducts of superovulated female ICR mice at 16 hours after hCG injection. The post-ovulatory aged oocytes were collected at 24 and 48 hours after hCG injection from in vivo and in vitro, respectively. Immunocytochemistry was performed on ${\beta}$-tubulin and acetylated ${\alpha}$-tubulin. The microtubules were localized in the spindle assembly, which was barrel-shaped or slightly pointed at its poles and located peripherally in the fresh ovulated oocytes. The frequency of misaligned metaphase chromosomes were significantly increased in post-ovulatory aged oocytes after 48 hours of hCG injection. The spindle length and width of post-ovulatory aged oocytes were significantly different from those of fresh ovulated oocytes, respectively. The staining intensity of acetylated ${\alpha}$-tubulin showed stronger in post-ovulatory aged oocytes than that in the fresh ovulated oocytes. In the aged oocytes, the spindles had moved towards the center of the oocytes from their original peripheral position and elongated, compared with the fresh ovulated oocytes. Microtubule organizing centers were formed and observed in the cytoplasm of the aged oocytes. On the contrary, it was not observed in the fresh ovulated oocytes. The alteration of spindle formation and chromosomes alignment substantiates the poor development and the increase of disorders from the post-ovulatory aged oocytes. It might be important to fertilize on time in ovulated oocytes for the developmental competence of embryos with normal karyotypes.

• Journal of Drive and Control
• /
• v.14 no.2
• /
• pp.23-29
• /
• 2017

In this study, a flexible multi-body dynamic simulation model of a knuckle boom crane is developed to evaluate the stress of spindle and rack gears under dynamic working conditions. It is difficult to predict potential critical damage to a knuckle boom crane if only the static condition is considered during the development process. To solve this issue, a severe working scenario (high speed with heavy load) was simulated as a boundary condition for testing the integrity of the dynamic simulation model. The crane gear model is defined as a flexible body so contact analysis was performed. The functional motion of a knuckle boom crane is generated by applying forces at each end of the rack gear, which was converted from hydraulic pressure measured for the experiment. The bending and contact stress of gears are theoretically calculated to validate the simulation model. In the simulation, the maximum stress of spindle and rack gears are observed when the crane abruptly stops. Peak impact force is produced at the contact interface between pinion and rack gears due to the inertia force of the boom. However, the maximum stress (bending/contact) of spindle and rack are under the yield stress, which is safe from damage. By using the developed simulation model, the experiment process is expected to be minimized.

• Journal of the Korean Society for Precision Engineering
• /
• v.18 no.8
• /
• pp.48-53
• /
• 2001

In order to increase productivity and efficiency, high-speed rotating machines become popular these days. The high-speed rotating machine is likely to vibrate and cause machine failure even though it has small unbalance. Therefore, a balancing technique is studied in this paper. Off-line balancing methods are inadequate to solve unbalance vibration problem occurring in the field due to flexible rotor effect, faster tool change, and shorter spin-up and down, etc. An active balancing is suggested to allow re-balancing of the entire rotating assembly in the machine when a tool is changed. This paper shows how to identify the dynamics of the system using influence coefficient and suggest an active balancing technique based on influence coefficient method for high-speed spindle system.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2004.05a
• /
• pp.748-752
• /
• 2004

Fluid dynamic bearings (FDBs) have been replacing ball bearings of the HDD spindle motor very rapidly. But there are several demerits of HDB, such as high friction torque, variable viscosity of the fluid lubricant depending on operating temperature, low stiffness, and etc. Eccentricity is one of the major parameters which affects the static and dynamic characteristics. As the static eccentricity is larger, the stiffness and the damping coefficients become bigger. But friction torque is relatively unaffected by the static eccentricity. This research proposes a new type of journal bearing with asymmetric journal grooves which results in better dynamic characteristics. The static and dynamic characteristics of the new journal bearing are investigated by solving the Reynolds' equation with FEM, and the transient analysis is performed to predict the dynamic behavior of rotor by solving the equations of motion of a HDD spindle system with Runge-Kutta method. The result shows that the proposed Journal bearings have much bigger stiffness and damping coefficients compared with the conventional symmetric ones. And consequently, it has smaller whirl radius and tilting angle.

• KSTLE International Journal
• /
• v.3 no.2
• /
• pp.79-83
• /
• 2002

Sealing an oil-air mixture plays important roles to have an enhanced lubrication for high speed spindle. High speed spindle requires non-contact type of sealing mechanism. Current work emphases on the investigation of the air jet effect on the protective collar type labyrinth seal. To improve the sealing capability of conventional labyrinth seal, air jet was injected against through the leakage flow, It has a combined geometry of a protective collar type and an air jet type. Both of a numerical analysis by CFD (Computational Fluid Dynamics) and experimental measurements are carried out to verify sealing improvement The sealing effects of the leakage clearance and the air jet magnitude are studied in various parameters. The results of pressure drop in the experiment match reasonably to those of the simulation by introducing a flow coefficient Effect of sealing improvement is explained as decreasing of leakage clearance by air jetting. Thus, sealing effect is improved by amount of air jetting even though clearance becomes larger.