• Title/Summary/Keyword: spiA

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Mutation of the invF Gene Encoding a Salmonella Pathogenicity Island 1 (SPI1) Activator Increases Expression of the SPI2 Gene, sseA (Salmonella Pathogenicity Island 1(SPI1)의 발현조절 유전자 invF의 변이가 SPI2 유전자(sseA)의 발현에 미치는 영향)

  • Han, Ah-Reum;Joe, Min-Ho;Kim, Dong-Ho;Baik, Sang-Ho;Lim, Sang-Yong
    • Microbiology and Biotechnology Letters
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    • v.40 no.1
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    • pp.70-75
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    • 2012
  • In Salmonella enterica, many genes encoded within Salmonella pathogenicity islands (SPI) 1 and 2 are required to cause a range of diseases in a variety of hosts. The SPI1-encoded regulator HilD activates both the SPI1 and 2 genes at different times during growth in Luria-Bertani (LB) media. In this study, the expression levels of hilD during growth in LB were investigated. The data suggest that hilD expression is induced in the early stationary phase and decreases in the late stationary phase, when sseA, an SPI2 gene, is maximally expressed. However, HilD could act as an activator of sseA expression in the late stationary phase despite being present at low levels. SseA expression was investigated in SPI1 regulator mutant strains, hilA, hilD and invF mutants. As expected, hilD mutation decreased sseA expression. However, we found that invF mutation caused a 1.5-fold increase in sseA expression in not only LB but also M9 minimal media, which is thought to resemble an intracellular environment. InvF overexpression restored sseA expression to wild-type levels in an invF mutant but did not cause an additional reduction in sseA expression. These results suggest that SPI1 controls SPI2 expression either positively or negatively.

Effects of pH, Electrolyte Concentrations, and Alginate Molecular Weights on Surface Hydrophobicity of Soy Protein Isolates (pH, 전해질의 농도 및 알긴산 분자량이 분리콩단백질의 표면소수성에 미치는 영향)

  • Lim, Yeong-Seon;Yoo, Byung-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.9
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    • pp.1285-1292
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    • 2016
  • Changes in surface hydrophobicity of soy protein isolate (SPI), which plays an important role in the functional characteristics of protein, were measured according to various SPI concentrations, pH levels, electrolytes concentrations, and alginate molecular weights by using 1-anilino-8-naphthalene sulfonic acid as a fluorescent probe. SPI surface hydrophobicity decreased as SPI concentrations increased. SPI surface hydrophobicity reached a maximum at pH 7.0. SPI surface hydrophobicity rapidly increased as the NaCl concentration of SPI solution increased up to 100 mM, and showed no large increases above 100 mM. However, SPI surface hydrophobicity radically decreased until the $CaCl_2$ concentration reached 50 mM and revealed no large variations above 50 mM. A similar trend was exhibited in the case of $MgCl_2$. As both the concentration and molecular weight of sodium alginate increased, SPI surface hydrophobicity decreased. The increasing rate of SPI surface hydrophobicity decreased as the molecular weight of sodium alginate increased.

Effect of Soybean Protein Isolate on the Properties of Noodle (분리대두단백질의 첨가가 제면적성에 미치는 영향)

  • Bae, Song-Hwan;Rhee, Chul
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1301-1306
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    • 1998
  • This study was conducted to investigate the effect of soybean protein isolate (SPI) on the properties of noodle which was made of composite flour blended with SPI extracted at acidic (pH 2.0, 3.0), neutral (pH 7.0) and alkaline (pH 10.0, 12.0) conditions. L-value of dry and cooked-moodle which were made of composite flour was lower than that of 100% wheat flour, but a and b-value wete higher than those of 100% wheat flour, Optimal cooking time of dry-noodle which was made of composite flour was longer than that of 100% wheat flour, but the weight, volume and water absorption of the cooked-noodle were lower than those of cooked-noodle of 100% wheat flour. Breaking force of dry-noodle which was made of composite flour blended with $SPI-2,\;SPI_3,\;SPI_{7}$, and $SPI-{10}$ was lower than that of 100% wheat flour, but the breaking force of dry-noodle which was made of composite flour blended with $SPI-{12}$ at level of 5% and 10% was same as that of 100% wheat flour. Springiness and cohesiveness of the cooked-noodle which was made of composite flour were same as those of 100% wheat flour, but chewiness and hardness were higher than those of 100% wheat flour.

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Correlation Analysis of Forest Fire Occurrences by Change of Standardized Precipitation Index (SPI 변화에 따른 산불발생과의 관계 분석)

  • YOON, Suk-Hee;WON, Myoung-Soo
    • Journal of the Korean Association of Geographic Information Studies
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    • v.19 no.2
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    • pp.14-26
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    • 2016
  • This study analyzed the correlation between the standardized precipitation index(SPI) and forest fire occurrences using monthly accumulative rainfall data since 1970 and regional fire occurrence data since 1991. To understand the relationship between the SPI and forest fire occurrences, the correlations among the SPI of nine main observatory weather stations including Seoul, number of fire occurrences, and log of fire occurrences were analyzed. We analyzed the correlation of SPI with fire occurrences in the 1990s and 2000s and found that in the 1990s, the SPI of 3 months showed high correlation in Gyeonggi, Gangwon, and Chungnam, while the SPI of 6 months showed high correlation in Chungbuk, and the SPI of 12 months showed high correlation in Gyeongnam, Gyenongbuk, Jeonnam, and Jeonbuk. In the 2000s, the SPI of 6 months showed high correlation with the fire frequency in Gyeonggi, Chungnam, Chungbuk, Jeonnam, and Jeonbuk, whereas the fire frequency in western Gangwon was highly correlated with the SPI of 3 months and, in eastern Gangwon, Gyeongnam, and Gyenongbuk, with the SPI of 1 month. In the 1990s, distinct differences in the drought condition between the SPI of 3 months and 12 months in the northern and southern regions of Korean Peninsula were found, whereas the differences in both the SPI of 1 month and 6 months were found in the Baekdudaegan region except western Gangwon since the 2000s. Therefore, this study suggests that we can develop a model to predict forest fire occurrences by applying the SPI of 1-month and 6-month data in the future.

The SPI Factors Affecting on Organizational Performance: The Prespective of the Moderating Effect of Organizational Maturity Level (조직성과에 미치는 SPI 영향요인에 관한 연구 :조직 성숙도의 조절효과 관점에서)

  • Kim, In-Jai;Seol, Kyung-Hwan
    • The Journal of Information Systems
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    • v.19 no.2
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    • pp.97-118
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    • 2010
  • Software Process Improvement(SPI) has been a challenging issue to software organizations. The factors affecting SPI were analysed for improving software development processes. Numerous studies suggested influencing factors, and assessment models were utilized for software improvement. This research suggested that SPI factors affecting on an organizational performance may vary according to the CMM(Capability Maturity Model) level. The moderating effects of organizational maturity level between the SPI factors and the organizational performance were empirically tested. This research found that all six SPI factors except for the resistance to change affected the organizational performance. The moderating effects of management support, goal alliances, participation, and effect analyses were empirically supported. These results can provide several implications or a practical guidance to academic and industry professionals.

A WblA-Binding Protein, SpiA, Involved in Streptomyces Oxidative Stress Response

  • Kim, Jin-Su;Lee, Han-Na;Lee, Heung-Shick;Kim, Pil;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.23 no.10
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    • pp.1365-1371
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    • 2013
  • The Streptomyces coelicolor wblA gene is known to play a negative role in both antibiotic biosynthesis and the expression of genes responding to oxidative stress. Recently, WhcA, a WblA ortholog protein, was confirmed to interact with dioxygenase-encoding SpiA ($\underline{s}$tress $\underline{p}$rotein $\underline{i}$nteracting with Whc$\underline{A}$) in Corynebacterium glutamicum. We describe here the identification of a SpiA ortholog SCO2553 protein ($SpiA_{sc}$) that interacts with WblA in S. coelicolor. Using heterologous expression in E. coli and in vitro pull-down assays, we show that WblA specifically binds $SpiA_{sc}$, and is influenced by oxidants such as diamide. These data indicate that the interaction between WblA and $SpiA_{sc}$ is not only specific but also modulated by the redox status of the cell. Moreover, a $spiA_{sc}$-disruption mutant exhibited a less sensitive response to the oxidative stress induced by diamide present in solid plate culture. Real-time RT-PCR analysis also showed that transcription levels of oxidative stress response genes (sodF, sodF2, and trxB) were higher in the $spiA_{sc}$-deletion mutant than in wild-type S. coelicolor. These results show that $SpiA_{sc}$ negatively regulates WblA during oxidative stress responses in S. coelicolor.

Estimation and assessment of long-term drought outlook information using the long-term forecasting data (장기예보자료를 활용한 장기 가뭄전망정보 산정 및 평가)

  • So, Jae-Min;Oh, Taesuk;Bae, Deg-Hyo
    • Journal of Korea Water Resources Association
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    • v.50 no.10
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    • pp.691-701
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    • 2017
  • The objective of this study is to evaluate the long-term drought outlook information based on long-term forecast data for the 2015 drought event. In order to estimate the Standardized Precipitation Index (SPI) for different durations (3-, 6-, 9-, 12-months), we used the observation precipitation of 59 Automated Synoptic Observing System (ASOS) sites, forecast and hindcast data of GloSea5. The Receiver Operating Characteristic (ROC) analysis and statistical analysis (Correlation Coefficient, CC; Root Mean Square Error, RMSE) were used to evaluate the utilization of drought outlook information for the forecast lead-times (1~6months). As a result of ROC analysis, ROC scores of SPI(3), SPI(6), SPI(9) and SPI(12) were estimated to be over 0.70 until the 2-, 3-, 4- and 5-months. The CC and RMSE values of SPI(3), SPI(6), SPI(9) and SPI(12) for forecast lead-time were estimated as (0.60, 0.87), (0.72, 0.95), (0.75, 0.95) and (0.77, 0.89) until the 2-, 4-, 5- and 6-months respectively.

소프트웨어 프로세스 개선의 성공요인이 조직성과에 미치는 영향: 조직성숙도의 매개효과의 관점에서

  • Kim, In-Jae;Seol, Gyeong-Hwan
    • 한국경영정보학회:학술대회논문집
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    • 2008.06a
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    • pp.995-1000
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    • 2008
  • Software Process Improvement(SPI) becomes an important issue at software quality. A research model is suggested on the basis of previous literature. The model includes success factors for SPI as independent variables and CMM levels as mediating variables, and defines organizational performance as dependent variables. This study basically investigates causational relationships among SPI success factors, CMM levels, and organizational performance through a structured equation model. A LISREL v8.72 for windows is used for statistical analysis. Even through success factors for SPI partially influences the CMM level, the CMM level is fully related to the level of organizational performance. The results show that the success factor for SPI in Korean companies are not equal to the factors abroad, but indicate that the CMM level is closely related to the organizational performance. These results will be helpful to academicians as well as to practitioners.

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The Performance Evaluation for PHY-LINK Data Transfer using SPI-4.2 (SPI-4.2 프로토콜을 사용한 PHY-LINK 계층간의 데이터 전송 성능평가)

  • 박노식;손승일;최익성;이범철
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.3
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    • pp.577-585
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    • 2004
  • System Packet Interface Level 4 Phase(SPI-4.2) is an interface for packet and cell transfer between a physical layer(PHY) device and a link layer device, for aggregate bandwidths of OC-192 ATM and Packet Over Sonet/SDH(POS), as well as 10Gbps Ethernet applications. In this paper, we performs the research for SPI-4.2. Also we analyze the performance of SPI-4.2 interface module after modeling using C programming language. This paper shows that SPI-4.2 interface module with 512-word FIFO depth is able to be adapted for the offered loads to 97% in random uniform traffic and 94% in bursty traffic with bursty length 32. SPI-4.2 interface module can experience an performance degradation due to heavy overhead when it massively receives small size packets less than 14-byte. SPI-4.2 interface module is suited for line cards in gigabit/terabit routers, and optical cross-connect switches, and SONET/SDH-based transmission systems.

Design of an SPI Interface for multimedia cards in ARM Embedded Systems (ARM 내장 임베디드 시스템용 멀티미디어카드를 위한 SPI 인터페이스 설계)

  • Moon, San-Gook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.2
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    • pp.273-278
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    • 2012
  • In this contribution, we design and implement an SPI hardware interface for the microprocessor to communicate with the MMC (Multi-Media Card) in an embedded system. Proposed architecture is compatible with the APB in AMBA bus architecture. Embedding OS in an embedded system means a big burden in terms of hardware and software ending up with performance decline. In this paper, we adopt the concept of SPI communication without using OS in the embedded system and implement in a form of FPGA chip. The designed SPI module was automatically synthesized, placed, and routed. Implementation was performed through the Altera FPGA and well operated at 25MHz clock frequency, which satisfied our target speed.