• The Plant Pathology Journal
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• 제17권1호
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• pp.1-8
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• 2001

The technique of plant transformation has started to show off its great power in the area of plant breeding by commercially successful introduction of transgenic varieties such as herbicide tolerant soybean and insect resistant corn in USA with an unimaginable speed. However, in contrast with the great success in the commercialization of herbicide tolerance and insect resistance, the transformation works on disease resistance has not yet reached the stage of full commercialization. This review surveys the current status of molecular breeding for plant disease resistance and their limits and problems. Some novel ideas and approaches in molecular breeding for disease resistance are introduced.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.20-20
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• 2022

A traditional wheat breeding program needs more than 12-13 years to develop a new cultivar. In recent years, 'Speed breeding (SB)' system, which uses extremely extended photoperiod (22 h), enabled up to 4-6 generations of spring wheat per year. However, since almost 70% of wheat cultivars are winter type, and over 95% of total cultivation area is for winter wheat in Korea, optimized vernalization treatment was essential for improving the SB system. Several vernalization temperatures and durations were tested with various genotypes, and the 4 weeks of 8-10 ℃ vernalization treatment was the most effective to develop 4 generations per year, for both spring and winter type wheat cultivars. This 'Speed vernalization (SV)' system followed by SB, allowed developing a new F₆ recombinant inbred lines (RILs) within 2 years. Among the 184 RILs, which derived from a cross between Jokoyung and Joongmo2008, two outstanding lines were selected for yield trial test, and then named Milyang52 and Milyang53. Compared to the traditional wheat breeding program, over 60% of the time was saved to develop these two lines. Marker-assisted selection and backcross were also combined with the SV system. YW3215-2B-1 (Jokoyung*3/Gamet), which has similar agronomic traits with Jokyoung and the same Glu-B1 allele with Garnet, was developed within 2.5 years. Thus, the SV system combined with molecular breeding technology would help breeders to make a new cultivar with less time and high efficiency.

• 한국작물학회지
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• 제65권4호
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• pp.365-376
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• 2020

미성숙배를 이용한 조직배양은 높은 재분화율로 인하여 밀 형질전환에 적용하기 위한 가장 좋은 방법이지만 매우 한정적인 수집시기와 생육조건으로 인해 이용하기 위한 시간의 제한과 많은 노동력이 집약되어있다. 따라서 안정적이고 지속적인 미성숙배 수집을 위해 스피드 브리딩 조건을 이용하여 출수가 촉진된 밀 이삭을 이용한 미성숙배 조직배양의 효율을 검정하였고, 연구결과는 다음과 같다. 1. 스피드 브리딩 조건의 출수기는 정상조건과 비교하여 36일에서 54일까지 단축되었으며, 개화 후 11일 된 영과에서 수집된 미성숙배 조직배양의 재분화율과 소식물체 출현 비율은 정상조건과 비교하여 유의한 차이가 없다는 것을 확인하였다. 2. 두 배양 조건에서 항산화 효소 활성 분석 결과 차이가 나타나지 않았으며, 연관 표지 유전자 발현 검정을 통하여 일장과 세포분열과의 관계를 검정하여 스피드 브리딩 조건에서 수집된 이삭의 안정성을 검정하였다. 3. 본 연구 결과는 스피드 브리딩 조건을 활용한 미성숙배 조직배양에 활용될 것이며 관련 유전자 발현 및 항산화 활성 검정 결과를 통해 검증된 안정적 미성숙배는 밀 형질전환 기술 개발에 활용될 수 있을 것으로 사료된다.

• 한국작물학회지
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• 제68권4호
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• pp.304-312
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• 2023

본 연구에서는 LED 광원을 이용한 세대단축 시스템을 콩에 적용하기 위한 조건을 설정하고자 하였다. 생태형을 고려하여 선정한 12개 품종을 대상으로 광질, 광량, 그리고 토양 조건을 검토함으로써 콩에 최적화된 세대단축 시스템 프로토콜을 설정하는 것을 목표로 하였고, 그 결과는 다음과 같다. 1. 10시간의 단일 광 조건에서, 광질 1:1:1비율일 때, 광량이 400 PPFD에서 900 PPFD, 1,300 PPFD로 증가함에 따라 개화일수, 평균 절간장, 경장은 감소한 반면, 절수, 협수, 립수는 증가하였다. 2. 상토에서의 재배는 배양토에 비해 개체 별 절수, 협수, 립수가 50% 이상 증가하였다. 3. 광질에서 적색광의 비율이 증가한 2:1:1비율 조건은 1:1:1비율 조건과 비교하여 개화일수가 약 15일 이상 소요되는 것으로 나타났다. 4. 상기 조건에 따라 세대단축 시스템을 적용하였을 경우 모든 품종에서 약 30일 내외로 개화가 확인되었으며, 60일에서 70일 이내에 8립 이상의 종자 수확이 가능하였다. 5. 본 연구 결과로 구축된 세대단축 시스템을 적용한다면 연간 최대 6세대의 진전이 가능하며, 디지털 육종과 유전자 편집과 같은 현대적인 작물 육종 기술과의 결합을 통해 새로운 작물 개발을 가속화시킬 수 있을 것이다.

• 한국환경생태학회지
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• 제26권6호
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• pp.876-883
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• 2012

맹꽁이의 번식활동에 영향을 주는 기상요인을 분석하기 위해 2008년부터 2011년까지 4년 동안 제주도 대정읍에 위치한 산란지에서 조사하였다. 분석요인은 강우량, 습도, 온도, 기압, 풍속으로 강우가 있는 날에 산란유무를 가지고 비교분석을 하였다. 분석결과 맹꽁이의 번식활동에 영향을 주는 요인은 강우량, 습도, 기압으로 나타났다. 이 중에서 가장 큰 영향을 주는 요인은 강우량으로 나타났다. 맹꽁이는 많은 비가 내릴 때 산란활동에 참가하는 것으로 나타났으며, 습도와 기압도 번식활동에 어느 정도 영향을 주는 것으로 분석되었다. 온도인 경우는 번식기간이 여름철이므로 이미 번식활동에 필요한 조건을 충족시킨 것으로 판단하였다. 따라서 맹꽁이도 온대지방에서 이루어진 다른 무미류 연구에서와 같이 강우량과 온도의 영향을 많이 받고 있다는 것이 확인되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권1호
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• pp.33-41
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• 2024

To investigate the effect of sawdust fermentation period and storage period after fermentation on development as food for P. brevitarsis larvae, individual and group breeding were conducted on berry sawdust and oak sawdust. In individual breeding, the growth period of P. brevitarsis larvae was reduced by 12 days from the 60-day fermentation of berry sawdust to the 40-day fermentation of berry sawdust, 30 days from the 90-day fermentation of oak sawdust, and the weight of the larvae was the heaviest. In group breeding, the time it takes for P. brevitarsis larvae to change from 1^st to 3^rd instar is about 30 days after hatching from 60-day fermentation of berry sawdust, while 90-day fermentation of oak sawdust took more than 60 days, so the growth speed was fast and the survival rate was good. The results of the farmhouse demonstration test were the same trend, and it was judged that it would be possible to produce P. brevitarsis larvae with berries fermented sawdust, and it would be advantageous in terms of economy. In addition, for both individual and group breeding, the growth period of P. brevitarsis larvae was longer as the storage period was longer, the weight of the larvae decreased, and the survival rate was no different. The development period of P. brevitarsis larvae was the longest in the storage period of 18 months for berries fermented sawdust, and the storage period of oak fermented sawdust was longer in the storage period of 12 months and 18 months. Therefore, considering the results of individual breeding and group breeding, the fermentation period was appropriate for 60 days for berries sawdust, and the storage period for berries sawdust was stable from 0 to 12 months, and 0 to 6 months for oak sawdust.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 추계학술대회
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• pp.121-121
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• 2020

• 한국작물학회:학술대회논문집
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• 한국작물학회 2023년도 춘계학술대회
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• pp.122-122
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• 2023

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.134-134
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• 2022

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.312-312
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• 2017

The study was conducted to select optimal materials for promoting germination rate, high sprouting vigor by priming treatment using PEG 6000 (water potential -0.5 to 2.0 Mpa) and Azolla extracts (0.1, 1.0, and 10.0%) extracted by ethanol, distilled, and/or hot water in colored rice cultivars. Each rice seed (three black rice cultivars and two red rice cultivars) was soaked 24 and 48 hr including untreated control. In black rice, Joseongheugchal rice cultivar, azolla ethanol extract (0.1%) induced highest germination rate, germination speed was taken to 5 days in distilled water and to 3days in Azolla extracts extracted hot water. Otherwise, degree of bacterial inhibition (number of colony, $10^3cfu$) in dry seed, water soaking for 24hr, soaking with fungicide for 24hr and 48hr, soaking with fungicide and aeration for 24hr and 48 hr was 22, 500, 95, and 0.46, respectively. In order to minimize fungal inhibition, a method can be chosen to combination of soaking fungicide and aeration for 48 hr. In seed priming treatments using growth pouch, seed soaking with fungicide did not affect change of germination percentage and germination speed, it delayed only 2 or 4day in the Joseongheugchal rice cultivar. It differs from rice cultivars and priming materials, Azolla extract(0.1 to 1%) promotes seed germination percentage in the Ilpum, Hongjinju, and Joseonghuegchal, in addition, germination in Jeogjinju cultivar was only promoted by PEG solution(10 to 20%), otherwise, it showed much lower or inhibited on the germination in Heugjinju and Sinmyungheugchal rice cultivars. In a paddy field trial, seedling establishment rate by applying PEG6000 and azolla extract did not show significantly statistical difference. When it compared with untreated control, seedling establishment rate was increased over 50% in priming treatments. Interestingly, seedling establishment rate under azolla extract (0.1%) extracted with ethanol was promoted over 2.5 times compared to the control in a black rice, Joseongheugchal and red rice, Jeogjinju.