• Journal of Photoscience
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• 제11권1호
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• pp.29-33
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• 2004

The mechanism of interaction of cyanocobalamin (CB) with bovine serum albumin (BSA) has been investigated by spectrofluorometric and circular dichroism methods. Association constant for the CB-BSA system showed that the interaction is non-covalent in nature. Binding studies in the presence of an hydrophobic probe, 8-anilino-l-naphthalene sulphonic acid, sodium salt (ANS) showed that there is hydrophobic interaction between CB and ANS and they do not share common sites in BSA. Stern-Volmer analysis of fluorescence quenching data showed that the fraction of fluorophore (protein) accessible to the quencher (CB) was close to unity indicating thereby that both tryptophan residues of BSA are involved in drug-protein interaction. The rate constant for quenching, greater than $10^{10}$ $M^{-1}$ $s^{-1}$, indicated that the drug binding site is in close proximity to tryptophan residue of BSA. Thermodynamic parameters obtained from data at different temperatures showed that the binding of CB to BSA involves hydrophobic bonds predominantly. Significant increase in concentration of free drug was observed for CB in presence of paracetamol. Circular dichroism studies revealed the change in helicity of BSA due to binding of CB to BSA.

• 천문학논총
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• 제32권1호
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• pp.45-47
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• 2017

The Infrared Camera onboard the AKARI satellite carried out spectroscopic observations with a grism mode named NG, whose wavelength coverage was $2.5-5.0{\mu}m$. We reinvestigate the current flux calibration for the NG grism mode, with which calculated flux density implausibly decreases at $4.9{\mu}m$ especially for red objects due to the second-order light contamination. We perform a new spectral response calibration using blue and red standard objects simultaneously. New response curves which contain both the first-and second-order light are able to separate each contribution consistently and useful for studies of red objects such as CO ro-vibrational absorption in active galactic nuclei.

• Biotechnology and Bioprocess Engineering:BBE
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• 제3권2호
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• pp.61-66
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• 1998

Glycolipids produced by Pseudomonas aeruginosa YPJ-80 were characterized by chromatographic and spectorscopic techniques as a mixture of two rhamnolipids. For recovery of glycolipids from the culture broth, various isolation methods including ultrafiltration, adsorption and solvent extraction were compared. Ultrafiltration showed the best results in terms of glycolipids recovery. Further purification for spectroscopic analysis was carried out by adsorption chromatography and preparative thin layer chromatography. From the spectroscopic analysis, such as IR spectroscopy. FAB-MS, 1H-NMR and 13C-NMR and hydrolysis analysis, the glycolipids were identified as L-${\alpha}$-rhamnopyranosyl-${\beta}$-hydroxydecanoly-${\beta}$-hydroxydecanoate and 2-O-${\alpha}$-L-rhamnopyranosyl-${\alpha}$-L-rhamnopyranosyl-${\beta}$-hydroxydecanoyl-${\beta}$-hydroxydecanoate. Monorhamnolipid and dirhamnolipid lowered the surface tension of water to 28.1 mN/M and 29.3 mN/m, respectively.

• Natural Product Sciences
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• 제15권2호
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• pp.106-109
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• 2009

Phytochemical investigations of the EtOAc-soluble fraction of the whole plants of Salvia plebeia using repeated column chromatography with preparative HPLC led to the isolation of a new phenylbutanone glucoside, 4-{4-0-[6-(4-hydroxybenzoyl)-0-${\beta}$-D-glucopyranosyl]-3-hydroxyphenyl}-butan-2-one (salviaplebeiaside, 1) along with two known phenolic compounds, rosmarinic acid methyl ester (2) and luteolin-7-0-${\beta}$-D-glucoside (3). The structures of these compounds were determined on the basis of spectroscopic methods including 1D-, 2D-NMR and MS spectrometry and comparison of spectroscopic data with those of values reported in the literatures.

• 한국안광학회지
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• 제13권3호
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• pp.57-60
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• 2008

목적: 펄스형 방전플라스마 포커스 장치에서 반경방향 플라스마 current sheath 의 속력을 측정하였다. 방법: 측정에는 시간분해 분광분석법과 로고프스키 코일이 사용되었다. 결과: 15 kV의 방전전압과 수십 torr의 He과 Ar의 기체기압에서 $10^5$ cm/s의 속력이 측정되었으며, 기체기압이 증가할수록 current sheath의 속력은 감소되었다. 결론: 최적조건인 수 torr의 기압에서는 $10^7$ cm/s의 속력이 나올 것으로 예상된다.

• Natural Product Sciences
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• 제20권2호
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• pp.130-134
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• 2014

An investigation was carried out to identify novel anti-cancer compounds from Korean indigenous plant extracts. Bioassay-guided fractionation and chemical investigation of the EtOAc extract from the leaves and stems of Stauntonia hexaphylla resulted in the isolation of two active compounds, hederagenin 3-O-${\alpha}$-L-arabinoside (1) and quercetin (2). The structures of these compounds were elucidated by spectroscopic methods, including UV, IR, MS, NMR techniques and compared with previous spectroscopic data. The cytotoxic effects of fractions and compounds on HCT116 human colon cancer cells were evaluated using the MTT assay. Quercetin showed a stronger anti-cancer effect when compared to hederagenin 3-O-${\alpha}$-L-arabinoside.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제18권2호
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• pp.77-105
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• 2014

Medical imaging techniques have evolved to expand our ability to visualize new contrast information of electrical, optical, and mechanical properties of tissues in the human body using noninvasive measurement methods. In particular, electrical tissue property imaging techniques have received considerable attention for the last few decades since electrical properties of biological tissues and organs change with their physiological functions and pathological states. We can express the electrical tissue properties as the frequency-dependent admittivity, which can be measured in a macroscopic scale by assessing the relation between the time-harmonic electric field and current density. The main issue is to reconstruct spectroscopic admittivity images from 10 Hz to 1 MHz, for example, with reasonably high spatial and temporal resolutions. It requires a solution of a nonlinear inverse problem involving Maxwell's equations. To solve the inverse problem with practical significance, we need deep knowledge on its mathematical formulation of underlying physical phenomena, implementation of image reconstruction algorithms, and practical limitations associated with the measurement sensitivity, specificity, noise, and data acquisition time. This paper discusses a number of issues in electrical tissue property imaging modalities and their future directions.

• Archives of Pharmacal Research
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• 제28권11호
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• pp.1239-1243
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• 2005

A new norditerpenoid and a known alkaloid were isolated from the alkaloidal fraction of the processed tubers of Aconitum carmichaeli. The structure of the new norditerpenoid alkaloid was elucidated as lipoforesaconitine (1) on the basis of spectroscopic analysis. The known norditerpenoid alkaloid was characterized as lipoyunanaconitine (2). In addition, a new flavonoid, 6'-O-acetylliquiritin (7), along with a known ceramide, (2S,3S,4R,8E)-2-[(2'R)-2'­hydroxylignoceroylamino]-8(E)-octadecene-1,3,4-triol (3), as well as a known steroid saponin, gracillin (8), and three known flavonoids, Iiquiritigenin (4), isoliquiritigenin (5), and liquiritin (6), were also isolated and characterized. All known compounds were isolated from this plant for the first time. The structures of the isolates were established by spectroscopic and chemical methods.

• 천문학회보
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• 제41권1호
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• pp.70.4-71
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• 2016

The links between super-massive black hole masses and their host galaxy properties are observed, indicating that black hole growth and host galaxy evolution are closely related. Reverberation mapping, which uses the time delay from the central black hole to broad line regions, is one of the best methods to estimate masses of black holes of active galactic nuclei (AGNs). However, only masses of about 50 black holes have been determined in reverberation mapping studies so far, and most of them are limited to optical luminosities below 10^45 erg/s due to the challenges of long-term time domain observations in both photometry and spectroscopy. In this project, we expand reverberation mapping samples to higher luminosities of > 10^44.5 erg/s at 0.1 < z < 0.35, that have expected time lags of 40 - 250 light days. Photometric (using LOAO 1-m and MDM 1.3-m) and spectroscopic (using MDM 2.4-m and Lick 3-m) monitoring campaigns are being conducted for a 3 year duration and 20 day cadence. Precedent photometric observations in 2015B show some targets with variability and follow-up spectroscopic observations are on-going. In this presentation, we introduce our project, present reverberation mapping simulation results, and preliminary results on photometry. These reverberation mapping masses of relatively high luminous AGNs will provide a strong constraint on black hole mass calibration, e.g., the single-epoch mass estimation.

• Bulletin of the Korean Chemical Society
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• 제25권6호
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• pp.889-894
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• 2004

Polyaddition reactions of 1,1-diethynyl-3-triethylsilyl-1-silacyclopent-3-ene with several organoborane derivatives have afforded the oligomeric materials containing organosilacyclic group and organoboron moiety along the oligomer main chains. All of these materials are soluble in THF as well as chloroform, and their molecular weights are in the range of 1,990/1,190-21,950/7,050 ($M_w/M_n$) with the polydispersity indexes of 1.67-3.43. The prepared oligomers are characterized by several spectroscopic methods such as $^1H,\;^{13}C, \;^{29}Si,\;^{11}B$ NMR and FTIR spectra along with elemental analysis. FTIR spectra of all the oligomers show that the new strong C=C stretching frequencies appear at 1599-1712 $cm^{-1}$, in particular. The UV-vis absorption spectra of the materials in THF solution exhibit the strong absorption bands at the ${\lambda}_{max}$ of 268-275 nm. The oligomeric materials show that the strong excitation peaks appear at the ${\lambda}_{max}$ of 255-279 nm and the strong fluorescence emission bands at the ${\lambda}_{max}$ of 306-370 nm. All the spectroscopic data suggest that the obtained materials contain both the organoboron ${\pi}$-conjugation moiety of C=C-B-C=C and the organosilacyclic group of 3-triethylsilyl-1-silacyclopent-3-ene along the oligomer main chains. The oligomers are thermally stable up to 162-200 $^{\circ}C$ under nitrogen.