• Title/Summary/Keyword: specific surface

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Microscopic Characteristics of the Olfactory Organ in the Gluttonous Goby Chaenogobius gulosus(Pisces, Gobiidae), Compared to Sympatric Intertidal Gobies (별망둑 the gluttonous goby Chaenogobius gulosus 후각기관의 해부, 조직학적 특성 및 동소 망둑어과 출현종들과의 비교연구)

  • Kim, Hyun Tae;Park, Jong Young
    • Korean Journal of Ichthyology
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    • v.33 no.1
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    • pp.1-7
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    • 2021
  • Using stereo, light, and scanning electron microscopes, we researched the anatomical and histological structure of Chaenogobius gulosus's olfactory organ and compared it to those of sympatric gobies Luciogobius guttatus and Favonigobius gymnauchen. Results revealed the following common characteristics: i) tubular anterior nostril (AN) and flat posterior nostril (PN), ii) a single longitudinal lamella, iii) two accessory nasal sacs (ANS, ethmoidal and lacrimal), iv) abundant sensory epithelium lymphatic cells (LC), v) an eosinophil cell, and vi) a ciliary length a quarter of the knob diameter in the olfactory receptor neuron (ORN). Some characteristics are specific to C. gulosus and different from the other two gobies: i) 0.5~1.0 mm AN and 0.2~0.5 mm PN (vs. 0.2~0.3 mm and 0.2~0.3 mm in L. guttatus; 0.2~0.4 mm and 0.1~0.3 mm in F. gymnauchen), ii) two ANS (vs. absence in L. guttatus; two in F. gymnauchen), iii) abundant LC (vs. low in L. guttatus and F. gymnauchen), iv) low density non-sensory cilia on the lamellar surface (vs. high in L. guttatus; low in F. gymnauchen), and v) a quarter ciliary length to knob diameter ratio in the ORN (vs. mixture of a quarter to equal ratio in L. guttatus; two or three times in F. gymnauchen). From these results, we confirmed the C. gulosus olfactory organ has adapted anatomically and histologically to the sand-rock tidal zone.

Generation of single stranded DNA with selective affinity to bovine spermatozoa

  • Vinod, Sivadasan Pathiyil;Vignesh, Rajamani;Priyanka, Mani;Tirumurugaan, Krishnaswamy Gopalan;Sivaselvam, Salem Nagalingam;Raj, Gopal Dhinakar
    • Animal Bioscience
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    • v.34 no.10
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    • pp.1579-1589
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    • 2021
  • Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×106 spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

Effect of Repeated Wet/Dry Cycles of Salt Solution on Flexural Performance of Steel Fiber Reinforced Concrete (반복적 염수침지가 강섬유 혼입 콘크리트의 휨성능에 미치는 영향)

  • Kim, Ji-Hyun;Choi, Yu-Jin;Chung, Chul-Woo
    • Journal of the Korea Institute of Building Construction
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    • v.22 no.6
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    • pp.553-564
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    • 2022
  • Concrete is a representative composite material that shows excellent performance in the construction field. However, it is a brittle and nonhomogeneous material and exhibits weak behavior against bending and tensile forces. To compensate for such weakens, fiber reinforcement has been utilized, and steel fiber has been recognized as one of the best material for such purpose. However, steel fiber can seriously affect the durability of concrete exposed to the marine environment due to the corrosion caused by chlorine ions. This study intended to evaluate the mechanical performance of steel fiber reinforce concrete during and after repeated wet/dry cycles in salt solution. According to the experimental results, there was no reduction in the relative dynamic modulus of concrete during the repeated wet/dry cycles in salt solution for 37 weeks. Flexural strength was not decreased after completion of repeated wet/dry cycles in salt solution. There was no sign of corrosion in steel fibers after visual observation of fractured surface. However, the flexural toughness was decreased, and this is because about half of the concrete specimen showed failure before reaching the maximum displacement of 3 mm. Although repeated wet/dry cycles in salt solution did not cause cracks in concrete through corrosion of steel fibers, specific attention is required because it can reduce flexural toughness of steel fiber reinforced concrete.

Angioimmunoblastic T-Cell Lymphoma with Polyclonal Proliferation of Plasma Cells: A Cautionary Note for Flow Cytometry Interpretations (유세포 분석의 주의사항: 혈관면역모세포성 T세포 림프종에서 관찰된 다클론성 형질세포)

  • Shin, Woo Yong;Bang, Hae In;Kim, Jung-Ah;Kim, Jieun;Park, Rojin
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.1
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    • pp.68-72
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    • 2022
  • Angioimmunoblastic T-cell lymphoma (AITL) is a lymphoproliferative disorder of mature T follicular helper cells. Atypical lymphoid cells were observed in the bone marrow of an 80-year-old woman, and the flow cytometric determined immunophenotypes of B-cells were unusual, that is, CD19+, CD20-, and CD22- with lambda light chain restriction. Initially, we suspected BM involvement of B-cell lymphoma based on the presence of abnormal B-cells. However, the patient was diagnosed with AITL involving BM. A re-analysis of flow cytometric immunophenotyping revealed a minor, aberrant T-cell population, and the lambda light chain restriction observed by surface staining was considered non-specific binding. This case demonstrates B-cells in patients with EBV-positive T-cell lymphoma may exhibit immunophenotypes resembling those of plasma cells, and that proliferation of abnormal B-cells or plasma cells could also potentially mask underlying T-cell lymphoma. A more integrated approach is required for accurate diagnosis.

Effects of Electrolyte Cation on Electrochemical Properties of Negative and Positive Electrodes in Aluminum-Air Batteries (알루미늄-공기 전지의 음극 및 양극의 전기화학적 특성에 미치는 전해질 양이온의 영향)

  • Lee, Seunghwan;Yoon, Sungjae;Choi, Weon-Kyung;Baeg, Changhyun;Jeong, Soon-Ki
    • Journal of Convergence for Information Technology
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    • v.12 no.2
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    • pp.134-141
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    • 2022
  • To improve the performance of aluminum-air batteries, it is very important to understand the effect of electrolytes on the electrochemical properties of electrodes. In this study, the effects of electrolyte cations on the electrochemical redox reactions proceeding at the negative and positive electrodes were investigated using electrolytes having the same anion but different cations such as NaCl, LiCl, CaCl2, and ZnCl2. It was confirmed by discharge test, scanning electron microscopy and X-ray diffraction analysis that electrolyte cations affect the discharge potential and specific capacity of the electrode. Precipitates were formed on the surface of the positive electrode by Ca2+ and Zn2+ ions, resulting in degradation of the performance of the positive electrode. In addition, Ca2+ ions passivated the negative electrode and accelerated the performance degradation. This suggests that the positive ions of the electrolyte have different effects on the electrochemical performance of the positive and negative electrodes.

Synthesis of Titanium Hydride Powder Via Magnesiothermic Reduction of TiCl4 in H2 gas Atmosphere (수소분위기 내 사염화타이타늄의 마그네슘 열환원을 이용한 수소화타이타늄 분말 합성)

  • Sung-Hun Park;So-Yeong Lee;Ho-Seong Lee;Jungshin Kang;Ho-Sang Sohn
    • Resources Recycling
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    • v.32 no.2
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    • pp.19-32
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    • 2023
  • A novel method for the synthesis of titanium hydride powder from titanium tetrachloride via the magnesiothermic reduction in an hydrogen gas atmosphere was investigated. To examine the influence of temperature on the formation of titanium hydride, the reduction was conducted at 1023~1123 K under 1 atm of hydrogen gas atmosphere for approximately 30 min. Subsequently, the titanium hydride powder was sintered by maintaining the temperature for 0~120 min, and the decrease in the oxygen concentration of the powder was investigated. The experimental results showed that TiH1.924 was produced at 1023 K, whereas mixtures of TiH1.924 and TiH1.5 were produced at 1073 K and 1123 K. In addition, the hydrogen concentration in the powder decreased with increasing temperature. The concentration of oxygen in the powder decreased with increasing temperature and sintering time owing to the decrease in the specific surface area of the powder. The minimum concentration of oxygen was 0.246 mass% when the mixture of TiH1.924 and TiH1.5 was obtained at 1073 K and a sintering time of 120 min.

GIS-based Spatial Zonations for Regional Estimation of Site-specific Seismic Response in Seoul Metropolis (대도시 서울에서의 부지고유 지진 응답의 지역적 예측을 위한 GIS 기반의 공간 구역화)

  • Sun, Chang-Guk;Chun, Sung-Ho;Chung, Choong-Ki
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.1C
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    • pp.65-76
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    • 2010
  • Recent earthquake events revealed that severe seismic damages were concentrated mostly at sites composed of soil sediments rather than firm rock. This indicates that the site effects inducing the amplification of earthquake ground motion are associated mainly with the spatial distribution and dynamic properties of the soils overlying bedrock. In this study, an integrated GIS-based information system for geotechnical data was constructed to establish a regional counterplan against ground motions at a representative metropolitan area, Seoul, in Korea. To implement the GIS-based geotechnical information system for the Seoul area, existing geotechnical investigation data were collected in and around the study area and additionally a walkover site survey was carried out to acquire surface geo-knowledge data. For practical application of the geotechnical information system used to estimate the site effects at the area of interest, seismic zoning maps of geotechnical earthquake engineering parameters, such as the depth to bedrock and the site period, were created and presented as regional synthetic strategy for earthquake-induced hazards prediction. In addition, seismic zonation of site classification was also performed to determine the site amplification coefficients for seismic design at any site and administrative sub-unit in the Seoul area. Based on the case study on seismic zonations for Seoul, it was verified that the GIS-based geotechnical information system was very useful for the regional prediction of seismic hazards and also the decision support for seismic hazard mitigation particularly at the metropolitan area.

A Study on the Meaning of Mugeuk(無極) and Taegeuk(太極) in Daesoon Thoughts (대순사상에서의 무극(無極)과 태극(太極) 그리고 대순(大巡)의 의미 고찰)

  • Park, Jae-hyun
    • Journal of the Daesoon Academy of Sciences
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    • v.22
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    • pp.433-469
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    • 2014
  • Identifying the ontological characteristics of "ultimate reality" is highly important in a specific religion or thought because it is believed to contain the logic that explain the wholistic phenomenon in the entire universe. The "ultimate reality" is called "Lee(理)", "Dao(道)", "Taegeuk; the Great Ultimate(太極)", "Mugeuk; NonUltimate(無極)", "Gong(空)", "Haven", "SangJe(上帝)", "God" in many different religions or thoughts. Taegeuk(太極), Mugeuk(無極) and Daesoon(大巡) are believed to contain the logic that explain the wholistic phenomenon in the entire universe in Daesoon Thoughts which we can see in the GooCheon SangJe(九天上帝)'s saying that "Even if the logic is so high, it comes from the surface of Taegeuk and Mugeuk and cannot be separated from every phenomenon in our life(理雖高出於太極无極之表 不離乎日用事物之間)" and Doju(道主) Cho, Jeong San's saying that "The reasion that Dao is called Dao is that it is settled to be Mugeuk and moved to be Taegeuk(道之謂道也者定而无極 動而太極)" and Dojeon(都典) Park Wu-dang's saying that "Daesoon(大巡) is circle(圓), circle is Mugeuk(無極), Mugeuk is Taegeuk(太極)". Mugeuk contains the logic of transcendence and Taegeuk contains the logic of immanance. And Nae-confucianism admit the Ultimate Reality or logic have both characteries of Mugeuk and Taegeuk and don't admit that Taegeuk comes from Mugeuk. Therefore, Mugeuk and Taegeuk are in complementary relations. These Nae-confucianism's idea about Mugeuk and Taegeuk corresponds with Deasoon Thought. Daesoon Thoughts also admit that Ultimate Reality, GooCheon SangJe can be understood with both Mugeuk and Taegeuk. That is, GooCheon SangJe, the Suprime God is transcendent from this world(Mugeuk) and also manage the whole universe using the logic of Taegeuk(太極). Daesoon(大巡) unite Mugeuk and Taegeuk using the concept of circulation. The logic of Mugeuk and Taegeuk is both two as well as one, and one as well as two. It is not dualism nor monism. It is non-dualism and super-dualism. These logic of coexistence is the core of Mugeuk and Taegeuk. And this logic is reflexed in Daesoon Thoughts' core proposition that "Daesoon(大巡) is circle (圓), circle is Mugeuk(無極), Mugeuk is Taegeuk(太極)".

Research on the Production of CO2 Absorbent Using Railway Tie Concrete Waste (콘크리트 철도 침목 폐기물을 활용한 CO2 포집제 제조 연구)

  • Gyubin Lee;Jae-Young Lee;Hyung-Jun Jang;Sangwon Ko;Hye-Jin Hong
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.11 no.3
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    • pp.260-266
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    • 2023
  • In recent years, excessive emissions of carbon dioxide(CO2) have become the cause of global climate change. Consequently, there has been significant research activity aimed at both removing and utilizing CO2. This study assesses the potential utilization of railway tie concrete waste, generated from railway infrastructure, as a CO2 absorption material and investigates the physicochemical properties before and after CO2 absorption to understand the CO2 removal mechanisms. Railway tie concrete waste primarily consists of Si(26.60 %) and contains 9.82 % of Ca. Compared to samples of Cement and Normal concrete waste, it demonstrated superior potential for use as a CO2 absorption material, with approximately 98 % of the Ca content participating in CO2 absorption reactions. Through Thermogravimetric Analysis(TGA) and X-ray Diffraction(XRD) analysis, it was confirmed that the carbonate reaction, where the Ca in railway tie concrete waste converts into CaCO3 through reaction with CO2 gas, is the primary mechanism for CO2 removal. Furthermore, Scanning Electron Microscopy(SEM) analysis revealed the formation of numerous CaCO3 particles with sizes less than 0.1 ㎛ after the CO2 absorption reaction. This transformation of large internal voids in the CO2 absorption material into mesopores resulted in an increase in the specific surface area of the material.

Synthesis of Polyimide Crosslinked Silica-based Aerogel with Enhanced Mechanical Properties and Its Physico-chemical Properties (폴리이미드 가교로 기계적 강도가 향상된 실리카 기반 에어로겔의 합성 및 물리화학적 특성 분석)

  • Kim, Jiseung;Choi, Haryeong;Kim, Taehee;Lee, Wonjun;Lee, Hong-Sub
    • Journal of the Microelectronics and Packaging Society
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    • v.29 no.4
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    • pp.9-14
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    • 2022
  • Silica aerogel is a porous material with a very low density and high specific surface area. Still, its application is limited due to its weak mechanical properties due to structural features. To solve this problem, a method of complexing it with various polymers has been proposed. We synthesized polyimide cross-linked silica aerogel by the sol-gel process to obtain high mechanical properties. Tetraethyl orthosilicate (TEOS) was used as a precursor to make silica aerogel, and 3- aminopropyltriethoxysilane (APTES) was used as a coupling agent for cross-linking polyimide. Polyimide was synthesized using pyromellitic dianhydride and 3,5-diaminobenzoic acid, and mechanical properties were improved by crosslinking polyimide with 10 repeating units in the polyimide chain using the reaction formula ${\frac{n_1}{n_2}}={\frac{n}{n+1}}$ To realize silica aerogel, polyimide having various weight ratios was added before gelation, resulting in a 19-fold or greater increase in maximum compressive strength compared to pure silica aerogel. From this study, an enhancement of silica aerogel could be enhanced through polymer cross-linking bonds.