• Plant Resources
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• v.3 no.3
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• pp.211-218
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• 2000

Intraspecific genetic relationship of 19 variation types of the Yam (Dioscorea alata) classified by their external morphological characteristics such as leaf and tuber shape were assessed by DNA using random and specific primer. Twenty two out of 113 primers (100 random[10-mer] primers, two 15 mer [M13 core sequence, and (GGAT)$_4$ sequence]) had been used in PCR-amplification. Only 12 primers, however, were success in DNA amplification in all of the analyzed plants, resulting in 93 randomly and specifically amplified DNA fragments. The analyzed taxa showed very high polymorphisms(69 bands, 71.0 %), allowing individual taxon to be identified based on DNA fingerprinting. Monomorphic bands among total amplified DNA bands of each primer was low under the 50%. Similarity indices between accessions were computed from PCR(polymerase chain reaction) data, and genetic relationships among intraspecific variations were closely related at the levels ranging from 0.66 to 0.90. These DNA data were not matched well with those of morphological characters since they were divided into two major groups at the similarity coefficient value of 0.70. Therefore, Grouping of species into variation types by mainly morphological charactistics was suggested unreasonable.

• International Journal of Fluid Machinery and Systems
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• v.3 no.4
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• pp.352-359
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• 2010

In recent years, the market has shown increasing interest in pump-turbines. The prompt availability of pumped storage plants and the benefits to the power system achieved by peak lopping, providing reserve capacity, and rapid response in frequency control are providing a growing advantage. In this context, there is a need to develop pumpturbines that can reliably withstand dynamic operation modes, fast changes of discharge rate by adjusting the variable diffuser vanes, as well as fast changes from pumping to turbine operation. In the first part of the present study, various flow patterns linked to operation of a pump-turbine system are discussed. In this context, pump and turbine modes are presented separately and different load cases are shown in each operating mode. In order to create modern, competitive pump-turbine designs, this study further explains what design challenges should be considered in defining the geometry of a pump-turbine impeller. The second part of the paper describes an innovative, staggered approach to impeller development, applied to a low head pump-turbine project. The first level of the process consists of optimization strategies based on evolutionary algorithms together with 3D in-viscid flow analysis. In the next stage, the hydraulic behavior of both pump mode and turbine mode is evaluated by solving the full 3D Navier-Stokes equations in combination with a robust turbulence model. Finally, the progress in hydraulic design is demonstrated by model test results that show a significant improvement in hydraulic performance compared to an existing reference design.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.28 no.6
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• pp.15-20
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• 2014

Recently, LED (Light Emitting Diode) application research is studying by using a specific wavelength. LED plant factory produced a lot of green plants in a closed spaces, so it should be taken to guard against harmful microbes. Until today, a lot of studies for green plant production in plant factory is proceed but there were no study on harmful microbes in plant factory. Thus, the analysis on sterilization for harmful microbes in plant factory were experimented using UV (Ultra Violet) LED with 282nm of wavelength. As a results on sterilization of three harmful microbes, 50% of sterilization efficiency was achieved after 2.5 hours, 97% was achieved after 12 hours of UV LED irradiation, respectively.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.10
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• pp.49-55
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• 2008

This paper presents a novel evaluation method of life expectancy of power system equipment. The life expectancy means expected remaining lifetime; it can be usefully utilized to maintenance planning, equipment replacement planning, and reliability assessment. The proposed method is composed of three steps. Firstly, a cumulative probability for future years is evaluated for targeted age year. Secondly, the cumulative probability is modeled by well-blown cumulative distribution function(CDF) such as Weibull distribution. Lastly, life expectancy is evaluated as the mean value of the model. Since the model CDF is established in the proposed method, it can also evaluate the probability of equipment retirement within specific years. The developed method is applied to examples of generators of combined cycle power plants to show its effectiveness.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.3 no.2
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• pp.141-145
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• 2002

Successive ion Exchange Column model was developed with the combination of mass action law and mole balance equation. consuming that ions entering the ion exchange bed pass the resin layer via consecutive ion exchange equilibrium. The application of the model to condensate polishing demineralizer in nuclear power plants indicates that the leakage of $Na^+$ and $Cl^-$ depends upon the degree of resin regeneration and that the ratio of specific ion concentration in Influent to in effluent is subject to the characteristics of resin and solution. The model can account for the local in-equilibrium with the correction of resin concentration and also can be applicable to a competitive ion exchange.

• The Plant Pathology Journal
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• v.21 no.4
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• pp.383-386
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• 2005

In 2003 typical phytoplasma symptoms of witches' broom and flower malformation were observed on statice (Limonium sinuatum) plants grown at commercial greenhouses in Busan, South Korea. The DNA extracted from the infected leaves was amplified using universal primer pair of Pl/P6 derived from conserved 16S rRNA gene of Mollicutes giving the expected Polymerase chain reaction (PCR) product of 1.5 kb. In the nested PCR assays, the expected DNA fragment of 1.1 kb was amplified with the specific primer pair 16Fl/Rl that was designed on the basis of aster yellows (AY) phytoplasma 16S rDNA sequences. The 1.1 kb PCR products were cloned and nucleotide sequences were determined. The sequences were identical to that of Onion yellows OY phytoplasma (GenBank accession no. D12569) isolated from Onion in Japan. Electron microscopy of thin sections of leaf veins showed phytoplasma bodies in the phloem. Statice witches' broom symptom occurred on statice in commercial greenhouses in Korea was confirmed as infection of AY phytoplasma by transmission electron microscopy observation, and by determination of 16S rRNA gene sequences of phytoplasma.

• The Plant Pathology Journal
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• v.18 no.6
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• pp.317-322
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• 2002

An isolate of Odontoglossum ringspot virus (ORSV) was identified from Cymbidium var. 'Grace Kelly' showing ringspot symptom on the floral and leaf parts, and was denoted as cymbidium ringspot isolate (ORSV-CR). In ultrathin sections of leaf tissue from diseased Cymbidium plants, clusters of virus particles were observed in the vacuole and cytoplasm. In the Western blot hybridization, the virus strongly reacted with ORSV-specific antiserum indistinguishable from ORSV, suggesting that the vims is serologically identical with ORSV. ORSV-CR sap was inoculated onto 20 species belonging to 12 genera. Systemic infection occurred in Cymbidium sp., Nicotiana benthamiana and N. clevelandii, the host of which was found to be different from that of ORSV-Cy, the Korean strain of ORSV. The analysis of coat protein (CP) gene showed that ORSV-CR was highly homologous to the known isolates of ORSV, with over 95.6% identity in amino acid level. Phylogenetic tree analysis of CP showed that ORSV-CR was clustered with the known ORSV isolates, suggesting that ORSV is a very stable tobamovirus.

• Journal of Power System Engineering
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• v.18 no.6
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• pp.106-112
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• 2014

The diesel engine, which has high compression ratio than other heat engines, has been using as the main power source of marine transport. Especially, since marine diesel engines offer better specific fuel consumption (SFC), it is environment-friendly compared to those used in other industries. However, attentio should be focused on emissions such as nitrous oxide ($N_2O$) which is generated from combustion of low-grade fuels. Because $N_2O$ in the atmosphere is very stable, the global warming potential (GWP) of $N_2O$ is 310 times as large as that of $CO_2$, and it becomes a source of secondary contamination after photo-degradation in the stratosphere. It has been hitherto noted on the $N_2O$ exhaust characteristics from stationary power plants and land transportations, but reports on $N_2O$ emission from the marine diesel engine are very limited. In this experimental study, a author investigated $N_2O$ emission characteristics by using changed diesel fuel components of nitrogen and sulfur concentration, assessed on the factors which affect $N_2O$ generation in combustion. The experimental results showed that $N_2O$ emission exhibited increasement with increasing of sulfur concentration in fuel. However, all kinds of nitrogen component additives used in experiment could not change $N_2O$ emission.

• Journal of the Korean Society for Precision Engineering
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• v.33 no.5
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• pp.341-348
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• 2016

The solenoid valve is used widely across various industries; however, solenoid valves for use in high-temperature environments have to be highly specified, such as those used in thermal power plants and steel mills. As such, we have developed a solenoid valve, using an already developed solenoid, to allow for more specific use. In this type of development method, use of 3D printing is very effective, allowing for a reduction in errors in design and production. This study includes a mathematical model of the solenoid valve. Then, the simulation from the mathematical model was performed using the AMESim (Advanced Modeling Environment for Simulation of Engineering Systems). We made a prototype valve using the simulation results and also measured the flow rate and dynamic performance.

• Molecules and Cells
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• v.39 no.7
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• pp.524-529
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• 2016

Controlling the production of diverse cell/tissue types is essential for the development of multicellular organisms such as animals and plants. The Arabidopsis thaliana root, which contains distinct cells/tissues along longitudinal and radial axes, has served as an elegant model to investigate how genetic programs and environmental signals interact to produce different cell/tissue types. In the root, a series of asymmetric cell divisions (ACDs) give rise to three ground tissue layers at maturity (endodermis, middle cortex, and cortex). Because the middle cortex is formed by a periclinal (parallel to the axis) ACD of the endodermis around 7 to 14 days post-germination, middle cortex formation is used as a parameter to assess maturation of the root ground tissue. Molecular, genetic, and physiological studies have revealed that the control of the timing and extent of middle cortex formation during root maturation relies on the interaction of plant hormones and transcription factors. In particular, abscisic acid and gibberellin act synergistically to regulate the timing and extent of middle cortex formation, unlike their typical antagonism. The SHORT-ROOT, SCARECROW, SCARECROW-LIKE 3, and DELLA transcription factors, all of which belong to the plant-specific GRAS family, play key roles in the regulation of middle cortex formation. Recently, two additional transcription factors, SEUSS and GA- AND ABA-RESPONSIVE ZINC FINGER, have also been characterized during ground tissue maturation. In this review, we provide a detailed account of the regulatory networks that control the timing and extent of middle cortex formation during post-embryonic root development.