• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.506-513
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• 2004

Spirulina produces $\gamma$-linolenic acid (GLA), an important pharmaceutical substance, in a relatively low level compared with fungi and plants, prompting more research to improve its GLA yield. In this study, metabolic flux analysis was applied to determine the cellular metabolic flux distributions in the GLA synthetic pathways of two Spiru/ina strains, wild type BP and a high­GLA producing mutant Z19/2. Simplified pathways involving the GLA synthesis of S. platensis formulated comprise of photosynthesis, gluconeogenesis, the pentose phosphate pathway, the anaplerotic pathway, the tricarboxylic cycle, the GLA synthesis pathway, and the biomass syn­thesis pathway. A stoichiometric model reflecting these pathways contains 17 intermediates and 22 reactions. Three fluxes - the bicarbonate (C-source) uptake rate, the specific growth rate, and the GLA synthesis rate - were measured and the remaining fluxes were calculated using lin­ear optimization. The calculation showed that the flux through the reaction converting acetyl­CoA into malonyl-CoA in the mutant strain was nearly three times higher than that in the wild­type strain. This finding implies that this reaction is rate controlling. This suggestion was sup­ported by experiments, in which the stimulating factors for this reaction $(NADPH\;and\;MgCl_{2})$ were added into the culture medium, resulting in an increased GLA-synthesis rate in the wild type strain.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.415-418
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• 2000

Productivity of a rice plant is greatly influenced by salt stress. One of the ways to achieve tolerance to salinity is to transfer genes encoding protective enzymes from other organisms, such as microorganisms. The bacterial gene, mtlD, which encodes mannitol-1-phosphate dehydrogenase (Mtl-DH), was introduced to the cytosol of a rice plant by an imbibition technique to overproduce mannitol. The germination and survival rate of the imbibed rice seeds were markedly increased by transferring the mtlD gene when it was delivered in either a pBIN19 or pBmin binary vector. When a polymerase chain reaction was performed with the genomic DNAs of the imbibed rice leaves as a template and with mtlD-specific primers, several lines were shown to contain an exogenous mtlD DNA. However, a reverse transcription (RT)-PCR analysis revealed that not all of them showed an expression of this foreign gene. This paper demonstrates that the growth and germination of rice plants transiently transformed with the bacterial gene, mtlD, are enhanced and these enhancements may have resulted from the experssion of the mtlD gene. The imbibition method empolyed in this study fulfills the requirements for testing the function of such a putative gene in vivo prior to the production of a stable transgenic plant.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.165-168
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• 1989

Ginseng plants in the 2nd year were grown under the shade (90-95%) of colored cellophane films at $15^{\circ}C$ in a natural light phytotron. Relative root grown (final/initial) was in decreasing order of blue, green, white, yellow and red. The growth of aerial part, especially stem length was poorest in blue light with relative root growth negatively correlated with stem length. In this relation each color showed specific domain. Stem length showed positive linear correlation with leaf area per plant in each and among light though it was not significant in red light. The content and ratio of chlorophyll a and b in leaf were in the decreasing order of White, Blue, Green, Red and Yellow. There is positive correlation between chlorophyll a and chlorophyll b, and between total chlorophyll and chl:a/b ratio. Blue color shade is expected to be beneficial for ginseng production.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.147-156
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• 2011

Efficient Agrobacterium-mediated genetic transformation of Scoparia dulcis L. was developed using Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pCAMBIA1301 with ${\beta}$-glucuronidase (GUS) (uidA) and hygromycin phosphotransferase (hpt) genes. Two-day precultured leaf segments of in vitro shoot culture were found to be suitable for cocultivation with the Agrobacterium strain, and acetosyringone was able to promote the transformation process. After selection on shoot organogenesis medium with appropriate concentrations of hygromycin and carbenicillin, adventitious shoots were developed on elongation medium by twice subculturing under the same selection scheme. The elongated hygromycin-resistant shoots were subsequently rooted on the MS medium supplemented with $1mg\;l^{-1}$ indole-3-butyric acid and $15mg\;l^{-1}$ hygromycin. Successful transformation was confirmed by PCR analysis using uidA- and hpt-specific primers and monitored by histochemical assay for ${\beta}$-GUS activity during shoot organogenesis. Integration of hpt gene into the genome of transgenic plants was also verified by Southern blot analysis. High transformation efficiency at a rate of 54.6% with an average of $3.9{\pm}0.39$ transgenic plantlets per explant was achieved in the present transformation system. It took only 2-3 months from seed germination to positive transformants transplanted to soil. Therefore, an efficient and fast genetic transformation system was developed for S. dulcis using an Agrobacterium-mediated approach and plant regeneration via shoot organogenesis, which provides a useful platform for future genetic engineering studies in this medicinally important plant.

• Journal of Information Technology Applications and Management
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• v.19 no.1
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• pp.37-46
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• 2012

The scheduling problem of large products like ships, airplanes, space shuttles, assembled constructions, and automobiles is very complex in nature. To reduce inherent computational complexity, we often design scheduling systems that the original problem is decomposed into small sub-problems, which are scheduled independently and integrated into the original one. Moreover, the steep growth of communication technology and logistics makes it possible to produce a lot of multi-nation corporation by which products are produced across more than one plant. Therefore vertical and lateral coordination among decomposed scheduling systems is necessary. In this research, we suggest an agent-based coordinating mechanism for multi-level scheduling systems in supply chain. For design of a general coordination mechanism, at first, we propose a grammar to define individual scheduling agents which are responsible to their own plants, and a meta-level coordination agent which is engaged to supervise individual scheduling agents. Second, we suggest scheduling agent communication protocols for each scheduling agent topology which is classified according to the system architecture, existence of coordinator, and direction of coordination. We also suggest a scheduling agent communication language which consists of three layers : Agent Communication Layer, Scheduling Coordination Layer, Industry-specific Layer. Finally, in order to improve the efficiency of communication among scheduling agents we suggest a rough capacity coordination model which supports to monitor participating agents and analyze the status of them. With this coordination mechanism, we can easily model coordination processes of multiple scheduling systems. In the future, we will apply this mechanism to shipbuilding domain and develop a prototype system which consists of a dock-scheduling agent, four assembly-plant-scheduling agents, and a meta-level coordination agent. A series of experiment using the real-world data will be performed to examine this mechanism.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.1
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• pp.14-30
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• 2006

It is difficult to secure public lots in the downtown area, school lots can be made the best use of important source to increase the area of green land in the city. The tree-planting campaign for schools has been in force since 1999 by the city of Seoul and at present; it is currently established as the plan of school parks. Thereupon, the purpose of this study is to present some ways to revitalize elementary schools to be used as vest pocket parks through the research of elementary schools, which are subject to the plan of school parks in Seoul. The results of this study are as below : The subjects to revitalize school parks as vest pocket parks are as follows. First, the land has to be utilized for security of space. Second, in addition to the front and back gate, another passage to the park has to be set up for easy access. Third, the fence has to be formed as a hedge used outside the park. Fourth, selecting plants, species of trees suitable for the landscape of school and taking into consideration the aspect of ecology. Finally, it is urgent to enact socially and economically proper standard of law by the new standards of area for school landscape considering not only the building law and regulations of general landscape, but also specific characteristics of school landscape.

• Journal of Ginseng Research
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• v.38 no.2
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• pp.123-129
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• 2014

Korean ginseng (Panax ginseng) and American ginseng (Panax quinquefolius) are widely used medicinal plants with similar morphology but different medicinal efficacy. Roots, flowers, and processed products of Korean and American ginseng can be difficult to differentiate from each other, leading to illegal trade in which one species is sold as the other. This study was carried out to develop convenient and reliable chloroplast genome-derived DNA markers for authentication of Korean and American ginseng in commercial processed products. One codominant marker could reproducibly identify both species and intentional mixtures of the two species. We further developed a set of species-unique dominant DNA markers. Each species-specific dominant marker could detect 1% cross contamination with other species by low resolution agarose gel electrophoresis or quantitative polymerase chain reaction. Both markers were successfully applied to evaluate the original species from various processed ginseng products purchased from markets in Korea and China. We believe that high-throughput application of this marker system will eradicate illegal trade and promote confident marketing for both species to increase the value of Korean as well as American ginseng in Korea and worldwide.

• The Journal of Fisheries Business Administration
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• v.14 no.1
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• pp.19-43
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• 1983

Mankind has made traditional use of various ocean resources in such several forms as fish, plants food, means of transportation, and military purpose, followed by the recent exploitation of offshore subsoil or sea-bed minerals, energies, and utilization of ocean space. These available ocean resources come from the marine natural environment which has a distinct feature in view of the relationship between human wants and their capacity to meet them. Though these socially basic resources however bring forth the so-called scarcity or differential rent, their communal nature of ownership dissipates free gifts of nature endowed to society as a whole. Thus to maximize these rents and social welfare thereof, rents should be secured and preserved through a well-defined arrangements of property-ownership as well as appropriate comparison of competing uses of marine resources, taking full cognizance of their irreversible adverse effects of a specific choice on the alternatives. Here I showed the sources of rent yielded from the multiple uses of navigation, fishery, mariculture, minerals, and recreation site, and also summarized the presently widely-known analytic tool to measure these rents with emphasis on due care of the telescopic faulty of the appraiser in charge, viewed from the communal point, Finally, as communal property is in strict sense owned by the public at large, notwithstanding the restricted communal ownership at government or local governments control, effectiveness of competition I expect should be kept while transferring claims of these resources from the legal owner to private enterprise as well as while extracting their rent by her. In particular, various national or social objectives look forward to tile maximization of social efficiency. Discretionary system in noncompetitive method thus, is exceptionally suggested because of probable suspicion from the public whether these resources are transferred as a give away or not in discretion. And these realized rent payments, I propose, should be wisely taken advantage of in advancement of scientific research in marine nature to bring an incremental rent therefrom successively.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.39 no.4
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• pp.72-80
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• 2016

The market size of plant projects in overseas is so large that domestic EPC project contractors are actively seeking the overseas projects and then trying to meet completion plans since successful fulfillment of these projects can provide great opportunities for them to expand into new foreign markets. International EPC projects involve all of the uncertainties common to domestic projects as well as uncertainties specific to foreign projects including marine transportation, customs, regulations, nationality, culture and so on. When overseas project gets off-schedule, the resulting uncertainty may trigger unexpected exceptions and then critical effects to the project performance. It usually require much more time and costs to encounter these exceptions in foreign sites compared to domestic project sites. Therefore, an exception handling approach is required to manage exceptions effectively for successful project progress in foreign project sites. In this research, we proposed a methodology for prediction and evaluation of exceptions caused by risks in international EPC projects based on sensitivity analysis and Bayesian Networks. First, we identified project schedule risks and related exceptions, which may meet during the fulfillment of foreign EPC projects that is performed in a sequence of engineering, procurement, preparatory manufacture, foreign shipping, construction, inspection and modification activities, and affect project performance, using literature review and expert interviews. The impact of exceptions to the schedule delay were also identified. Second, we proposed a methodology to predict the occurrence of exceptions caused by project risks and evaluate them. Using sensitivity analysis, we can identify activities that critically affect schedule delay and need to focus by priority. Then, we use Bayesian Networks to predict and evaluate exceptions. Third, we applied the proposed methodology to an international EPC project example to validate the proposed approach. Finally, we concluded the research with the further research topics. We expect that the proposed approach can be extended to apply in exception management in project management.

• Journal of Soil and Groundwater Environment
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• v.22 no.1
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• pp.49-58
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• 2017

Biotic ligand model (BLM) and species sensitivity distribution (SSD) were used to determine the site-specific Cu threshold concentration (5% hazardous concentration; HC5) in soil pore water. Model parameters for Cu-BLM were collected for six plants, one collembola, and two earthworms from published literatures. Half maximal effective concentration ($EC_{50}\{Cu^{2+}\}$), expressed as $Cu^{2+}$ activity, was calculated based on activities of major cations and the collected Cu-BLM parameters. The $EC_{50}\{Cu^{2+}\}$ varied from 2 nM to $251{\mu}M$ according to the variation in environmental factors of soil pore water (pH, major cation/anion concentrations) and the type of species. Hazardous activity for 5% (HA5) and HC5 calculated from SSD varied from 0.076 to $0.4{\mu}g/L$ and 0.4 to $83.4{\mu}g/L$, respectively. HA5 and HC5 significantly decreased with the increase in pH in the region with pH less than 7 due to the decrease in competition with $H^+$ and $Cu^{2+}$. In the region with pH more than 7, HC5 increased with the increase in pH due to the formation of complexes of Cu with inorganic ligands. In the presence of dissolved organic carbon (DOC), Cu and DOC form a complex, which decreases $Cu^{2+}$ activity in soil pore water, resulting in up to 292-fold increase in HC5 from 0.48 to $140{\mu}g/L$.