• 영어어문교육
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• 제6호
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• pp.1-24
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• 2000

Listening comprehension is an integrative and creative process of interaction through which listeners receive speakers' production of linguistic or non-linguistic knowledge. Compared with reading comprehension, it may arouse difficulties and thus impose more burdens on foreign learners. The Audio-Lingual Method focused primarily on speaking. Mimicry, repetition, rote memory, and transformation drills actually interfered with listening comprehension. So learners lost interest and were not highly motivated. Improving listening comprehension requires continual attentiveness and interest. Listening skill can be extended systematically only when students are frequently exposed to a wide range of listening materials with an affective, cultural, social, and psycholinguistic approach. Therefore, teachers should help students learn how to comprehend intactly the overall meaning of intended messages. The literature on teaching listening skill suggests various useful activities: TPR, dictation, role playing, singing, picture recognition, completion, prediction, seeking specific information, summarizing, labeling, humor, jokes, cartoons, media, and so on. Practical classroom teaching necessitates a systematic procedure in which students should take part in meaningful tasks/activities. In addition to this, learners must practice listening comprehension trough a self-study process.

• 분석과학
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• 제27권3호
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• pp.121-139
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• 2014

In this review, we will discuss aptamer technologies including in vitro selection, signal transduction mechanisms, and designing aptamers and aptazyme for label-free biosensors and catalysts. Dye-displacement, a typical label-less method, is described here which allows avoiding relatively complex labeling steps and extending this application to any aptamers without specific conformational changes, in a more simple, sensitive and cost effective way. We will also describe most recent and advanced technologies of signaling aptamer and aptazyme for the various analytical and clinical applications. Quantum dot biosensor (QDB) is explained in detail covering designing and adaptations for multiplexed protein detection. Application to aptamer array utilizing self-assembled signaling aptamer DNA tile and the novel methods that can directly select smart aptamer or aptazyme experimentally and computationally will also be finally discussed, respectively.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.696-701
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• 2002

This paper describes some results of change detection in Tokyo metropolitan area, Japan , using the Landsat TM data, and methods to quantify the ground cover classes. The changes are analyzed using the measures of not only conventional spectral classes but also a set of landscape indices to describe spatial properties of ground cove types using fractal dimension of objects, entropy in the specific windows defining the neighbors of focusing locations. In order eliminate the seasonal radiometric effects on TM data, an automated class labeling method is also attempted. Urban areas are also delineated automatically by defining the boundaries of the urban area. These procedures for urban change detection were implemented by the unified image computing methods proposed by the author, they can be automated in coherent and systematic ways, and it is anticipated to automate the whole procedures. The results of this analysis suggest that Tokyo metropolitan area was extended to the suburban areas along the new transportation networks and the high density area of Tokyo were also very much extended during the period between 1985 and 1995.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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• pp.290-293
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• 2005

This paper proposes and evaluates a new approach to detect ships as targets from Radarslit-l SAR (Synthetic Aperture Radar) imagery in the vicinity of Korean peninsula. To be more specific, a labeling technique and morphological filtering in conjunction with some other methods are employed to automatically detect the ships. From the test, the ships are revealed to be detected. For ground truth data, information from a radar system is used, which allows assessing accuracy of the approach. The results showed that the proposed approach has the high potential in automatically detecting the ships

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2005년도 춘계학술대회 논문집
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• pp.147-152
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• 2005

This paper proposes an evaluates a new approach to detect ships as targets from Radarsat-1 SAR (Synthetic Aperture Radar) imagery in the vicinity of Korean peninsula. To be more specific, a labeling technique and morphological filtering in conjunction with some other methods are employed to automatically detect the ships. From the test, the ships are revealed to be detected. For ground truth data, information from a radar system is used, which allows assessing accuracy of the approach. The results showed that the proposed approach has the high potential in automatically detecting the ships.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권3호
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• pp.191-195
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• 2000

Quantitation of N7-methyldeoxyguanosine (N7-MedG) produced in the in vitro N-methly-N-nitrosuourea (NMU) action on thymus DNA has been achieved by enzymatic degradation, liquid chromatoraphic separaphic separation and desorption chemical ionization tandem mass spectrometry. In conjunction with the resolving power HPLC in the separation of isomers, desorprion chemical ionization tandem mass spectrometry has utilized in determining modified nucleosides at low levels using a stable-isotope labled compound as an internal reforence. The quantitative estimation of N7-methyldeoxyguanosine was previously established by an independent HPLC analysis of methylated calf thymus DNA. A sensitive and specific methodogy for the quantitation of N7-MedG at the picomole level using HPLC combined with tandem mass spectrometry without radioisotope labeling process is presented. The potential of the liquid chromatoraphic tandem mass exposure to methlation agents in vitro.

• 대한방사성의약품학회지
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• 제1권1호
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• pp.46-52
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• 2015

Herein we present the synthesis of $^{68}Ga$-labeled gold nanoparticles for in vivo PET imaging. A novel chelator DTPA-Cys was easily prepared from diethylenetriaminepentaacetic dianhydride in high yield. The ${\alpha}_v{\beta}_3$ integrin receptor targeted gold nanoparticle probe was synthesized by using DTPA-Cys, polyethylene glycol and cRGD peptide. $^{68}Ga$ labeling of cRGD conjugated gold nanoparticle was carried out at $40^{\circ}C$ for 30 min. Observed radiochemical yield was more than 75% as determined by radio-TLC and the probe was purified by centrifugation. In vitro stability test showed that 90% of $^{68}Ga$-labeled gold nanoparticle probe was stable in FBS for 1 h. Those results demonstrated that $^{68}Ga$-labeled gold nanoparticle could be used as a potentially useful probe for specific tumor imaging.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 C
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• pp.1319-1321
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• 2006

This research aims to develop the multiple channel electrochemical DNA chip that has the above characteristic and be able to solve the problems. At first, we fabricated a high integration type DNA chip array by lithography technology. It is able to detect a plural genes electrochemically after immobilization of a plural probe DNA and hybridization of non-labeling target DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic system.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 추계학술대회 논문집 전기물성,응용부문
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• pp.51-53
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• 2002

This research aims to develop a multiple channel electrochemical DNA chip using micro-fabrication technology. At first, we fabricated a high integrated type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the sold electrodes. Then target DNAs were hybridized by an electrical force. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in the anodic peak current. Therefore, it is able to detect a various genes electrochemically after immobilization of a various probe DNA and hybridization of label-free DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

• Journal of the korean society of oceanography
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• 제31권2호
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• pp.97-106
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• 1996

Advantages and disadvantages of four radioisotopic methods, $^{3}H$-thymidine, $^{3}H$-adenine, $^{3}H$-leucine, and $^{35}S$-sulfate, for measuring bacterial production were reviewed. The maior issues discussed in production methods were: (1) whether all the actively growing bacteria take up the radiolabeled (organic) tracers; (2) how each target molecule should be purified (nonspecific labeling); and (3) how important the determination of the precursor pool specific activity is (internal isotope dilution). Since all the radioisotoic methods have their own advantages and disadvantages, careful consideration must be paid in choosing the radioisotope according to the conditions of each environment investigated.