• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1173-1180
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• 1997

Hepatotoxicity of caffeine and acetaminophen was investigated in this study. Special attention was paid to the effect of vitamins on the reduction of hepatotoxicity caused by the chemicals. Rat hepaocytes isolated by two-step perfusion method were cultured in two differents methods-suspension, monolayer cultures-, and exposed to caffeine and/or acetaminophen for 24hrs. Caffeine or acetaminophen exhibited no significant hepatotoxicity in terms of intracellular glutathione(GSH) level and lipid peroxidation(MDA), but GSH level was significantly decreased after administrated acetaminophen, and the toxicity caused by the chemicals showed a dose-dependent manner. The synergistic effect of caffeine and acetaminophen was observed when both caffeine and acetaminophen were supplemented to culture medium. At the concentration 1mM, caffeine enhanced the intracellular GSH depletion and MDA formation by 63% and 64%, respectively, compared to single supplementation of 10mM acetaminophen in culture medium. This hepatotoxicity induced membrane integrity loss was observed by lightmicroscope on the simultaneous administration of caffeine and acetaminophen in monolayer cultured hepatocytes. Co-supplementation of vitamins with caffeine/acetaminophen to culture medium results in the protection of hepatocytes from hepatotoxic attach by caffeine/acetaminophen. Especially, vitamin E was superior to vitamin C and $\beta$-carotene from the standpoints of GSH depletion and MDA formation. From this results, it has been speculated that vitamin E may play a role of antioxidant scavenging radicals produced from acetaminophen. Taken all together, in vitro culture system like monolayer culture of hepatocytes may be a useful tool for the evaluation of hepatotoxicity or protection ability of food ingredients.

• Biomolecules & Therapeutics
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• v.21 no.1
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• pp.54-59
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• 2013

In this study, we investigated the effect of (-)-epigallocatechin-3-gallate (EGCG), a major component of green tea catechins from green tea leaves, on activities of cyclooxygenase (COX)-1 and thromboxane synthase (TXAS), thromboxane $A_2$ ($TXA_2$) production associated microsomal enzymes. EGCG inhibited COX-1 activity to 96.9%, and TXAS activity to 20% in platelet microsomal fraction having cytochrome c reductase (an endoplasmic reticulum marker enzyme) activity and expressing COX-1 (70 kDa) and TXAS (58 kDa) proteins. The inhibitory ratio of COX-1 to TXAS by EGCG was 4.8. These results mean that EGCG has a stronger selectivity in COX-1 inhibition than TXAS inhibition. In special, a nonsteroid anti-inflammatory drug aspirin, a COX-1 inhibitor, inhibited COX-1 activity by 11.3% at the same concentration ($50{\mu}M$) as EGCG that inhibited COX-1 activity to 96.9% as compared with that of control. This suggests that EGCG has a stronger effect than that of aspirin on inhibition of COX-1 activity. Accordingly, we demonstrate that EGCG might be used as a crucial tool for a strong negative regulator of COX-1/$TXA_2$ signaling pathway to inhibit thrombotic disease-associated platelet aggregation.

• 대한공업교육학회지
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• v.44 no.1
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• pp.1-22
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• 2019

The purpose of this study is to examine the mediating effects of resilience in the relationship between parent support and career decision level of specialization technical high school students and to find out ways to improve the level of desirable career guidance and counseling. The research questions are as follows: First, what is the level of parent support, career decision level, and resilience of special technical high school students? Second, what is the relationship between parent support, career decision level and resilience of high school students? Third, the resilience of resilience plays a mediating role in the relationship between parent support and career decision level of special technical high school students. In order to achieve the purpose of the study, the theoretical background related to parent support, career decision level, and resilience of special technical high school students was investigated and the test tool was produced. The results of the study are as follows. Parental support, career decision level, and resilience were highly correlated. There was a significant correlation between parent support and career decision level, resilience and career decision level, and emotional support and verbal encouragement were more than 50% of the career decision level. In terms of mediating effect, the effect of parent support on career decision level was found to be partially mediated by overall resilience and sub - factors such as control, positive, and sociality. Therefore, in order to improve the quality of career guidance counseling, a program linked to the school and the home should be conducted considering both domestic factors and internal factors.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.52
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• pp.119-135
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• 2011

Korea's trading volume is expected to surpass USD 1 trillion in 2011. Korean economy achieves this largely due to its dependence on export and enhanced technological capacity and product quality. Improved recognition of Korean enterprises in the global arena also helped. However, the largest reason behind theses could be found in exporters' ceaseless marketing endeavors and continued government supports. Today, more and more people become to rely on trade exhibitions to boost export effectively. Trade exhibitions are employed as a useful tool to attract buyers and enter a market. This is because such exhibitions' marketing effect and professional aspects. South Korean export relies on large conglomerates for most part. However, 95% of the country's entire industry are small and medium-sized companies. This means that SMEs' export has a huge impact on the national economy. Therefore, as a way to improve SME export and minimize their losses, we need to use trade exhibitions more actively. The overseas policy insures only promotional activities regarding foreign exhibition and fair (including international events held in Korea), foreign distributor, shopping mall, home shopping, etc. and does not include air fare, traffic cost in the local place, accommodation cost and dining expenditures as costs to be excluded mentioned above. It is not easy for them to take part in events abroad if they have to pay for such expenses. If full financial support is difficult, the Korea trade insurance corporation still may give a certain level of aid for successful exporters to engage in marketing activities abroad more actively and further stimulate SMEs' export.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.1
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• pp.19-26
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• 1991

Ames test using special strains which are histidine requiring mutant of Salmonella typhimufium , is widely utilized as short-term bioassay to evaluate the genotoxicity of chemicals. This method requires the liver microsome(5-9 fraction) to provide mammalian metabolism of the compounds, Therefore, the mutagenic potency of the chemicals is affected by not only the intrinsic properties of them but also the efficiency of the in vitro microsomal activation system. For this reason, the complex mixtures such as environmental pollutants from occupational sources, natural products or cigarette smoke condensates(CSC) , might be often appeared the false results. Induction of sister chromatid exchange(SCE) in cultured cells is known as another sensitive and powerful tool for the measurement of genotoxicity and the method has also an advantage which is able to apply to the in vitro and in vivo systems. In the present study, the inducibilities of revertant colonies in tester strain TA98 and SCE in human Iymphocytes by positive controls and total particulated materials(TPM) obtained from various brand(domastic and imported) cigarettes were compared in order to investigate whether the results in Ames test are in agreement with those in SCE analysis. The mutagenic activities of well known mutagens such as B(a)P showed excellent dose-response in the both methods although the induction mechanism was different each other, but cyclophosphamide resulted such effect only in SCE analysis. Most TPM tested showed a similar pattern in the mutagenic activities in those methods. However, only two(one imported brand and one domestic sample cigarettes) among the TMP obtained from various cigarettes appeared the higher induction in SCE than Ames test.

• Composites Research
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• v.13 no.6
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• pp.39-46
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• 2000

Electrical discharge machining (EDM) cuts metal by discharging electric current across a thin gap between tool and workpiece. Electrical discharge wire cutting, a special form of EDM, uses a continuously moving conductive wire as an electrode, and is widely used for the manufacture of punches, dies and stripper plates. In the wire cutting process, the moving wire is usually supported by cantilever arm and wire guides. As the wire traveling speed has been increased in recent years to improve productivity, the vibration of the cantilever arm occurs, which reduces the positional accuracy of the machine. Therefore, the design and manufacture of the cantilever arm with high dynamic characteristics have become important as the machining speed increases. In this paper, the cantilever arm for guiding the moving wire was designed and manufactured using carbon fiber epoxy composite in order to improve the static and dynamic characteristics. Specimens for the composite cantilever arm were manufactured and tested to investigate the effect of the number of reinforcing plies and length fitted to steel flange on the load capacity. Also, the finite element analysis using layer and contact elements was performed to compare the calculated results with the experimental ones. From the results, the prototype of the composite cantilever arm for the electrical discharge wire cutting machine was manufactured and the static and dynamic characteristics were compared with those of the conventional steel cantilever arm.

• Proceedings of the KSMRM Conference
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• 2003.10a
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• pp.97-97
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• 2003

Contrast-enhanced MR angiography (CE-MRA) gradually occupies its position as a primary evaluation tool forsteno-occlusive disease of supra-aortic cervical arteries. It has several advantages over time-of-flight (TOF) technique such as shorter imaging time, less saturation effect, and less flow- and motion-related artifacts. Diverse methods of k-space sampling, imaging sequences, and strategies for image acquisitiontiming have been introduced since its early clinical application. Especially, methods of k-space sampling and image acquisition timing are very important to achieve maximal arterial enhancement and suppress venous signal while maintaining large scan coverage and high spatial resolution. In addition, regardless of several advantages over TOF technique, it still has a tendency to overestimate the degree of stenosis in patients with carotid or vertebralartery disease. In this exhibit, we will overview the current techniques of CE-MRA with special attention to methods of k-space sampling and image acquisition timing. We will also discuss diagnostic accuracy of CE-MRA in patients with supra-aortic cervical artery stenosis and artifacts frequently misinterpreted as steno-occlusive lesion on CE-MRA.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.04b
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• pp.400-407
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• 1995

The straightness property is one of fundamental geometric tolerances to be strictly controlled for guideways of machine tools and measuring machines. The staightness measurement for long guideways was usually difficult to perform, and it needed additional equipments or special treatment with limited application. In this paper, a new approach is proposed using the profile matching technique for the long guideways, which can be applicable to most of straghtness measurements. An edge of relativelly sthort length is located along a divided section of a long guideway, and the local straightness measurement is performed. The edge is then moved to the next section with several positions overlap. After thelocal straightness profile is measured for every section along the long guideway with overlap, the global straightness profile is constructed using the profile matching technique based on theleast squares method. The proposed techinique is numerically tested for two cases of known global straightness profile arc profile and irregular profile and those profiles with and without random error intervention, respectively. When norandom errors are involved, the constructed golval profile is identical to the original profile. When the random errors are involved, the effect of the number of overlap points are investigated, and it is also found that the difference between the difference between the constructed and original profiles is very close to the limit of random uncertainty with juist few overlap points. The developed technique has been practically applied to a vertical milling machine of moving table type, and showed good performance. Thus the accuracy and efficiency of the proposed method are demonstrated, and shows great potential for variety of application for most of straightness measuirement cases using straight edges, laser optics, and angular measurement equipments.

• Journal of Korean Society of Transportation
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• v.26 no.1
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• pp.137-144
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• 2008

It is important to know pedestrian volume to carry out pedestrian safety analysis and pedestrian friendly design. However, it is too difficult to come across research work related to pedestrian volume analysis in the field of transport, due to lack of interests on pedestrian movement. Most transport research has been focused on vehicles and highways rather than pedestrian. On the other hand, in the field of urban studies, there comes an effective tool to estimate pedestrian volumes using Space Syntax theory. This theory twins out to be effective and economic because it only requires network information, which is easy to acquire from maps and field survey. However, this method is different in the way representing networks from the way that is common in the field of transport. To make up for this point, this paper develops a novel measure for estimating pedestrian volume using Dial's algorithm, and applies the model in the two test networks; Insadong and Soongryemoon networks. The application results reveals that developed measure is an effective tool to explain pedestrian volume; a correlation coefficient between the measure and pedestrian volume is 0.713 in Insadong and 0.492 in Soongryemoon, and the goodness of fit($R^2$) of regression models are 0.893 in Insadong and 0.671 in Soongryemoon. This estimation method is significantly less complicated to estimate the effect of a pedestrian network change than Space Syntax theory, which requires special softwares not readily available.

• Journal of the Korea Convergence Society
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• v.12 no.6
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• pp.225-232
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• 2021

The purpose of this study is to identify the psychological characteristics of the elderly and to investigate the variables that affect the life satisfaction of the elderly. To achieve this purpose, psychological characteristics such as self-esteem, alienation, loss, and depression were selected. A survey was conducted on the elderly living in Gwangju Metropolitan City. A total of 218 copies were collected and 203 copies were analyzed, excluding poor responses. To draw the results of the research, quantitative analysis was conducted through questionnaires, and SPSSWIN 21.0 statistical program was used as an analysis tool. The analysis methods were frequency and ratio analysis, technical statistical analysis, bivariate correlation analysis, and linear regression analysis. The results of the analysis are as follows: First, the relationship between the psychological characteristics of the elderly showed significant correlations among the self-esteem, alienation, loss, depression, and life satisfaction of the elderly. Second, for the elderly, alienation and depression had a negative effect on life satisfaction, and self-esteem had a positive effect on life satisfaction. However, loss did not have a significant effect on life satisfaction. In conclusion, it suggests that it is necessary to develop and operate policies and programs to improve self-esteem and reduce alienation and depression in order to improve the quality of life of the elderly.