• Fisheries and Aquatic Sciences
• /
• v.13 no.4
• /
• pp.332-339
• /
• 2010

Maturity and spawning of the snailfish, Liparis ochotensis was investigated based on samples collected by gill net in the East Sea of Korea from December 2008 to December 2009. The average total length of L. ochotensis was 63.4 cm and 64.1 cm for females and males, respectively. Gonadosomatic index (GSI) began to increase in July, reaching a maximum in November, then began to decrease in December. Monthly changes in the GSI values of L. ochotensis were similar for females and males. Annual reproductive cycle of this species can be divided into five successive stages in females: early growing stage (May), late growing stage (June to July), mature stage (August to September), ripe and spent stage (September to December), and recovery and resting stage (January to April). Males show four successive stages; growing stage (May to July), mature stage (August to September), ripe and spent stage (September to December), and recovery and resting stage (January to April). Relationship between total length (TL) and fecundity (F) was F=$0.00003TL^{4.002}$ ($R^2$ = 0.703), and F increased with TL. Total length at 50% group maturity was estimated to be 55.8 cm. Our findings suggest that the spawning period takes place from September to December, with the main spawning period occurring from October to December.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.39 no.5
• /
• pp.391-397
• /
• 2006

This study correlated changes in estradiol-l7$\beta$ ($E_2$), testosterone (T), 17$\alpha$,20-dihydroxy-4-pregnen-3-one (DHP), and vitellogenin (VTG) levels with changes in the gonadosomatic index (GSI) and ovarian histology during the annual reproductive cycle of the wild marbled sole, Limanda yokohamae. Synchronous oocyte development occurs in this fish. Ovary maturity was classified into four periods, based on histological observations: the spawning (December to February), post-spawning (February to April), recovery (May to August), and vitellogenic (September to November) periods. Seasonal changes in the GSI were inversely correlated with water temperatures and reflected the degree of ovarian maturity. Plasma VTG levels were correlated with changes in the GSI, which increased from September to a peak in January, and levels remained comparatively high until February. Estradiol-17$\beta$ was at baseline levels (<0.11 ng/mL) during the spring and summer, and peaked rapidly (1.55$\pm$0.445 ng/mL) from October to January. Plasma T and DHP levels had a similar profile; they rose markedly during the spawning period and remained low (or were not detectable) from spring through autumn. These data indicate that changes in plsama steroid hormones and VTG levels are correlated with the annual ovarian activity of the marbled sole. Based on these results and published reports, it appears that in this species DHP is the most important maturation-inducing steroid and that T is also related to final maturation.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.55 no.6
• /
• pp.927-937
• /
• 2022

The reproductive characteristics of Pacific anchovy, Engraulis japonicus were investigated using samples randomly collected from the southern waters of Korea between January and December 2021. We analyzed monthly changes in maturity stages, gonadosomatic index (GSI), egg diameter, fecundity, and fork length (FL) at sexual groups maturity of 50%, 75% and 97.5%, and sex ratio (female:male). GSI value showed two peaks in May and August; however, microscopic analysis of E. japonicus gonad tissue revealed that the spawning period was from March to October. The main spawning seasons for the entire populations were from April to June and August to September. The overall sex ratio was female-biased (1 females, 0.89 male). The fecundity (F) of female anchovies ranged from 429 eggs at an FL of 8.0 cm to 13,824 eggs at an FL of 14.9 cm and the relationship between FL and F was 0.0788FL^4.422. The FL at 50% group maturity was 8.26 cm for female anchovies. Based on the results, we suggest that the reproductive cycle in Pacific anchovies may be related to pulses in water temperature and the sexual cycle of the fish.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.17 no.6
• /
• pp.529-535
• /
• 1984

This study aims at obtaining the basic data for the development of pen shell culture at Yoja Bay, the southwest coast of Korea. The authors dealt with the annual gonadal changes of the pen shell, Atrina pectinata japonica, to find out the gametogenesis, reproductive cycle and spawning season, and to investigate the fluctuation in the larval occurence in Yoja Bay. From July 1983 to June 1984, an average of 20 individuals as specimens were monthly collected by commercial fishing dredge near Manwol island located at the mouth of Yoja Bay. The degree of gonadal development was determined by the histological observations as resting spent stage, early developmental stage, after developmental stage, early spawning stage, after spawning stage and degenerative stage. According to these degrees of gonadal development, annual reproductive cycle of the pen shell population was determined. From July to August, the gonads were changed through degenerative into resting stage and, in September and October, they became entirely empty gonads. From November they showed the first sign of gametogenesis developing very slowly. Nevertheless, the developing gametes did not increase in number probably owing to a phagocytic phenomenon by phagocytes which appeared in the gonad during this stage. Some individuals started spawning in April and in May majarity of individuals were in spawning stage. In June, majority of the individuals showed signs of degenerative stage. Therefore, the authors came to a conclusion that the pen shells in Yoja Bay spawn chiefly in May. And this is also supported by the result of the survey on the planktonic occurrences of the pen shell larvae. Namely, there were no larvae at all in April, only a few in May and many in June by vertical water sampling.

• Animal cells and systems
• /
• v.11 no.2
• /
• pp.227-234
• /
• 2007

Spermatogenesis, the reproductive cycle, and the size at first sexual maturity in male Mactra chinensis were investigated by cytological and histological observations. The spermatozoon exhibits a primitive type morphology and is similar to those of other bivalves in that it contains a short midpiece with four mitochondria surrounding the centrioles. The morphologies of the sperm nucleus type and the acrosome shape of this species are cylindrical and modified cap-like, respectively. The spermatozoon is approximately $40-45\;{\mu}m$ in length including the sperm nucleus (about $1.46\;{\mu}m$), acrosome (about $1.20\;{\mu}m$) and tail flagellum. The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. The spawning period of this species lasts from June to September, and the main spawning occurs in July and August, when the seawater temperature is greater than $20^{\circ}C$. The percentage of individual male clams at first sexual maturity was 56.5% for those whose shell lengths were 35.1-40.0 mm, and 100% for over 45.1 mm. Accordingly, harvesting clams <35.1 mm in shell length could potentially cause a drastic reduction in recruitment, and a measure indicating a prohibitory fishing size should be taken for adequate fisheries management.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2001.02a
• /
• pp.46-49
• /
• 2001

R. Philippinarum is dioecious and oviparous. In the early vitellogenic oocyte, the Golgi apparatus and mitochondria present in the perinuclear region are involved in the formation of lipid droplets and in lipid granule formation. In the late vitellogenic oocyte, the endoplasmic reticulum, mitochondria in the cytoplasm are involved in the formation of proteid yolk granules. At this time, exogenous lipid granular substance and glycogen particles in the germinal epithelium are passed into the ooplasm of oocyte through the microvilli of the vitelline envelope. Ripe oocytes are about 55-60 $\mu$ m in diameter. The spawning period was once a year between early June and early October, and the main spawning occurred between July and August when seawater temperature was approximately 20 C. The reproductive cycle of this species can be categorized into five successive stages: early active stage (February to March), late active stage (April to May), ripe stage (April to August), partially spawned stage (June to October), and spent/inactive stage (August to March). Gonad developmental phases by histological qualitative analysis showed similar results with those of quantitative image analysis.

• The Korean Journal of Malacology
• /
• v.16 no.1_2
• /
• pp.35-41
• /
• 2000

We investigated the gonad index (GI), gonadal development, the reprodutive cycle and first sexual maturity of the venus clam, Cyclina sinensis by histological observation. Samples were collected from the intertidal zone of Komso Bay, Chollabuk-do, west coast of Korea, from April 1998 to March 1999. This clam is dioecious and oviparous. Monthly changes in the gonad index (GI) showed a similar pattern to the reproductive cycle. Ripe oocytes are about 90-100 ㎛ in diameter. The spawning period was between early July to September, and the main spawning occurred between July and August when the sea water temperature was over 20$\^{C}$ The reproductive cycle of this species can be divided into five successive stages: early active stage (February to April), late active stage (March to June), ripe stage (April to August), partially spawned stage (July to October), and spent / inactive stage (September to February). Percentages of first sexual maturity of female and male clams measuring 26.1-30.0 ㎜ in shell length were 53.3% and 62.5%, respectively, and 100% for the clams > 41.0 ㎜. It is assumed that both sexes begin reproduction at about two years age.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.42 no.1
• /
• pp.41-47
• /
• 2009

The seasonal reproductive cycle of Gomphina melanaegis collected in the coastal area of Jumunjin, between April 2006 and March 2007, was analyzed. Maturation cycle parameters such as the gonad index (GI), ovarian egg diameter, frequency of developmental stages, protein content, and RNA/DNA variation in the gonads were analyzed monthly for the 40 samples. According to the indices from histological sections, the frequency of gonad developmental stages, and the oocyte diameter, this clam has a long-term partial spawning pattern from March to October. However, GI and nucleic acid values showed that the mature stage is from March to July and that the main spawning season is August. The peak RNA and DNA contents were good indicators of sexual maturation in females and males, respectively. The variation in protein content corresponded with the RNA/DNA ratios.

• Korean Journal of Ichthyology
• /
• v.12 no.1
• /
• pp.62-70
• /
• 2000

Annual reproductive cycle of the file fish, Stephanolepis cirrhifer (Temminck et Schlegel), was histologically investigated. Samples were collected monthly in the coastal waters of Chungmun, south of Cheju Island, Korea from July 1997 to June 1999. In males and females of S. cirrhifer, the values of GSI (gonadosomatic index) were similar to the changes of water temperature and day length. GSI values in males and females were reached the maximum in June. Reproductive cycle could be divided into the following successive stages: in females, growing stage (February to May), mature stage (May to June), spawning stage (June to August), degenerative and resting stage (September to December), and in males, growing stage (February to April), mature stage (May to June), spent stage (June to August), degenerative and resting stage (September to January), respectively. Throughout histological observation of the ovary in the spawning period, the species belong to an asynchronous and multiple spawner.

• The Korean Journal of Malacology
• /
• v.18 no.2
• /
• pp.67-76
• /
• 2002

Gametogenesis, reproductive cycle, egg diameter composition and gonad index of the black-lined limpet, Cellana nigrolineata, were histologically examined. Specimens collected from the rocky intertidal zone of Hamdeok, Jeju from July 1997 to June 1998. The black-lined limpet has an unpaired gonad located at the ventro-anterior part, underneath the visceral mass. The gonad index increased from May when water temperature increased and reached maximum in August. It began to decrease from September thereafter, maintain a low value from January to April. The following reproductive cycle were classified based on the monthly changes of the histological features and sizes of oocytes in the gonad: recovery stage (November to April), active stage (March to June), ripe stage (May to October), spent stage (August to December). Spawning occur once a year between September and October. The black-lined limpet appeared to be gonochorism, neither sex change nor hermaphroditie.