• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.324-327
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• 2009

A new sprout-soybean cultivar, "Galchae" was developed at the National Institute of Crop Science (NICS) in 2008. Galchae was selected from a cross between YS1287 and Jinju#1. The preliminary, advanced, and regional yield trials to evaluate the performance of Iksan 64 were carried out from 2005 to 2008. This cultivar has a determinate growth habit with purple flower, brown pubescence, brown seed coat, brown hilum, rhomboid leaflet shape and small seed size (8.4 grams per 100 seeds). The maturity date of "Galchae" is 6 days later than the check variety, "Dawon". It has good seed quality for soybean-sprout and resistance to lodging. This cultivar has resistance to soybean mosaic virus (SMV) and necrotic symptom (SMV-N). The average yield of "Galchae" was 2.51 ton per hectare in the regional yield trials (RYT) for double cropping carried out for two years from 2007 to 2008.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.478-484
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• 2018

A soybean cultivar "Chamol" for double cropping for use as soy-paste and tofu was developed using a pedigree method in 2011 as a cross between "Shinpaldal2" and "Keunol." A promising line, SS99502-2B-89-1-3-4-1-1, was selected and designated as "Milyang210". It was promising and showed good results from regional yield trials (RYTs) for 3 years from 2009 to 2011 and released with the name "Chamol." It has a determinate growth habit, white flowers, gray pubescence, yellow seed coat, yellow hilum, spherical seed shape, and large seeds (27.7 g per 100 seeds). The maturity date of "Chamol" was September 18 (100 day growing period) in RYT and it is suitable for double cropping with winter crops such as onion. "Chamol" was resistant to bacterial pustule and soybean mosaic virus and tolerant to lodging in fields. Furthermore, the average yield of "Chamol" was 2.51 ton/ha in the regional yield trials conducted for 3 years from 2009 to 2011.

• Journal of Applied Biological Chemistry
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• v.58 no.4
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• pp.325-332
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• 2015

Rapeseed (Brassica napus) is now the second largest oilseed crop after soybean. Cold temperature tolerance is an important agronomic trait in winter rapeseed that determines the plant's ability to control below freezing temperatures. To improve cold tolerance of rapeseed plants, an expression vector containing an Barley Ice recrystallization inhibition protein (HvIRIP) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into rapeseed plants. Transgenic expression of HvIRIP was proved by southern- and northern-blot analyses. The level of freezing tolerance of transgenic $T_3$ plants was found to be significantly greater than that of wild-type rapeseed plants by freezing assay. Proline accumulation during cold stress was also highly induced in the transgenic rapeseed plants. The transgenic plants exhibited considerable tolerance against oxidative damage induced by cold stress. Our results indicated that heterologous HvIRIP expression in transgenic rapeseed plants may induce several oxidative-stress responsive genes to protect from cold stress.

• Journal of Food Hygiene and Safety
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• v.24 no.1
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• pp.19-26
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• 2009

This study was undertaken to analyze the hygienic problems of group food services and to predict the outbreak patterns of future food-borne diseases. A delphi survey with 20 experts identified the main causes of food-borne outbreaks in group food services as improper hygienic management of raw food materials, washing of worker's hands, dividing the spaces and unsanitary retail storage. Vibrio parahaemolyticus, Escherichia coli (EPEC), non-typhoid Salmonella serotypes, Staphylococcus aureus, Escherichia coli (ETEC), norovirus, and the hepatitis A virus all have potential to cause outbreaks of food-borne disease. We analyzed the daily food use and the possibility of food-borne outbreaks in school food services for fruits, milk, fish, pork, eggs, and meat as raw food materials, and bibimbab, soybean sprouts muchim, spinach namul, cucumber sengchae, jabchae, and pork bulgogi as prepared food items. Frozen (${\leq}\;-20^{\circ}C$) and refrigerated ($0{\sim}10^{\circ}C$) processed foods are popular items in group food services. Their storage, heating, and chemical sanitization methods are potential sources of food disease outbreaks. Our results can be applied to a well-organized hygiene control system and can be used to develop menus for preventing food-borne outbreaks.

• Journal of Food Hygiene and Safety
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• v.30 no.2
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• pp.150-158
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• 2015

This article reports the development of an effective test procedure for detection of norovirus (NoV) in foods of diverse matrices. In this study, target foods included fermented milk, soybean paste, powders made from uncooked grains and vegetables, sesame leaves preserved in soy sauce, pickled mooli, and mooli. Viral recovery varied depending on the food matrices or elution buffers tested. Buffers were compared to determine effective elution buffers from artificially virus-contaminated foods. The conventional test procedure for concentrating viruses from food (elution-polyethylene glycol(PEG) precipitation-chloroform-PEG precipitation) was modified to save time by eliminating one PEG precipitation step. The modified procedure (elution-chloroform-PEG precipitation) was able to concentrate viruses more effectively than the conventional procedure. It also removed RT-PCR inhibitors effectively. The modified procedure was applied to target food for genogroup II NoV detection. NoV RNA was detected at the initial inoculum levels 3.125-12.5 RT-PCR units per 10-25 g tested food. The use of this newly established procedure should facilitate detection of low levels of norovirus in diverse foods.