• 한국육종학회지
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• 제40권2호
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• pp.97-100
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• 2008

Lectin protein and Kunitz trypsin inhibitor (KTI) protein of mature soybean seed are a main antinutritional factor in soybean seed. The Le gene controls a lectin protein and Ti gene controls the KTI protein in soybean. Ti locus has been located on linkage group 9 in the classical linkage map of soybean. Position of Le locus on linkage map was not identified. Genetic relationship between Ti locus and Le locus could be useful in soybean breeding program for the genetic elimination of these factors. The objective of this study was to determine the independent inheritance or linkage between Ti locus and Le locus in soybean seed. Two $F_2$ populations were developed from three parents (Gaechuck#1, T102, and PI548415). The $F_1$ seeds from Gaechuck#1 (titiLeLe) ${\times}$ T102 (TiTilele) and Gaechuck#1 (titiLeLe) ${\times}$ PI548415 (TiTilele) were obtained. The lectin and KTI protein were analysed from $F_2$ seeds harvested from the $F_1$ plants to find independent assortment or linkage between Ti locus and Le locus. The segregation ratios of 3 : 1 for Le locus (129 Le_ : 44 lele) and Ti locus (132 Ti_ : 41 titi) and were observed. The segregation ratios of 9 : 3 : 3 : 1 (95 Le_Li_ : 34 Le_titi: 37 leleTi_ : 7 leletiti) between Le gene and Ti gene in $F_2$ seeds were observed. This data showed that Ti gene was inherited independently with the Le gene in soybean. These results will be helpful in breeding program for selecting the line with lacking both KTI and lectin protein in soybean.

• Journal of Plant Biology
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• 제22권1_2호
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• pp.1-4
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• 1979

Wild soybean plants(Glycine ussuriensis) collected from Korea(47 strains) and abroad(41 strains) were grown under field condition and classified according to the growth habit. Seeds were analyzed for protein content. The results were as follows: 1. Wild soybean plants were classified into three groups each by seed size, growth habit of stem, leaf size and flowering time. 2. Seed protein was higher in the strains with large leaves, tendril, stem or late flowering. 3. Glycine gracilis, supposed to be the intermediate species between cultivated and wild soybean(according to the stem growth habit, straight or semitendril, and seed size) was medium in protein content of seeds. 4. The average protein content of Glycine ussuriensis, 43.2%, was highest in comparison with those of others such as G. gracilis, 37.5%, or G. max, 36.2%. 5. One hundred seed weight of Amphicarpaea trisperm, similar to the wild soybean, was almost same as Glycine ussuriensis, but protein content of this species was lower than wild soybeans.

• 한국육종학회지
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• 제43권2호
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• pp.115-119
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• 2011

Soybean proteins are widely used for human and animal feeds worldwide. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are present in the raw mature soybean. P34 protein, referred as Gly m Bd 30K, has been identified as a predominant immunodominant allergen. The objective of this research is to identify the genetic mode of P34 protein for the improvement of soybean cultivar with a very low level of P34 protein. Two $F_2$ populations were developed from the cross of "Pungsannamulkong" ${\times}$ PI567476 and "Gaechuck2ho" ${\times}$ PI567476 (very low level of P34 protein). Relative amount of P34 protein was observed by Western blot analysis. The observed data for the progeny of "Pungsannamulkong" and PI567476 were 133 seeds with normal content of P34 protein and 35 seeds with very low level of P34 protein (${\chi}^2=1.157$, P=0.20-0.30). For the progeny of "Gaechuck#1" and PI567476, the observed data were 177 seeds with normal content of P34 protein and 73 seeds with very low level of P34 protein (${\chi}^2=2.353$, P=0.10-0.20). From pooled data, observed data were 310 seeds with normal content of P34 protein and 108 seeds with very low level of P34 protein (${\chi}^2=0.156$, P=0.50-0.70). The segregation ratio (3:1) and the Chi-square value obtained from the two populations suggested that P34 protein in mature soybean seed is controlled by a single major gene. Single gene inheritance of P34 protein was confirmed in 32 $F_2$ derived lines in $F_3$ seeds, which were germinated from the low level of P34 protein obtained from the cross of "Pungsannamulkong" and PI567476. These results may provide valuable information to breed for new soybean line with low level of P34 protein and identification of molecular markers linked to P34 locus.

• 한국육종학회지
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• 제41권4호
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• pp.612-615
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• 2009

Lipoxygenase and Kunitz trypsin inhibitor protein are the main antinutritional factor in mature soybean seed. A new soybean cultivar, "Gaechuck#2" with yellow seed coat, lipoxygenase2,3-free and Kunitz trypsin inhibitor protein-free was developed. It was selected from the population derived from the cross between "Jinpumkong2ho" and C242. Plants of "Gaechuck#2" have determinate growth habit with purple flowers, tawny pubescence, yellow seed coat, yellow hilum, oval leaflet shape and brown pods at maturity. Seed protein and oil content on a dry weight basis were 40.7% and 18.7%, respectively. It has shown a resistant reaction to soybean necrosis, soybean mosaic virus, Cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. Gaechuck#2 matured in 4 October with plant height of 54cm and a 100-seed weight of 24.4g. Average Yield of Gaechuck#2 was 230 - 250 kg/10a in 2005 - 2007.

• 기술사
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• 제18권2호
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• pp.16-22
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• 1985

This study was carried out to evaluate the quality of soybean sprouts grown under three different sources of light. The soybean was germinated under light through blue (251 ux), red (751 ux) and white (50 to 200 lux) polyethylene films at 25$^{\circ}C$ for 10hr./day. Vitamin C, chlorophyll, cellulose and total protein contents were determined and texture was evaluated by tasting soybean sprouts soup. protein pattern in the soybean sprouts were investigated using polyacrylamide gel electrophoresis. Vitamin C, chlorophyll and cellulose contents were increased by white light intensity. The texture of the sprouts grown under white light (50 lux, 100 lux) was better than darkness, but fought under 200 lux. Vitamin C contents of soybean sprouts grown under various sources of light (in order of light : blue > white > red) were higher than theses of ones grown in the darkness. Biosynthesis of chlorophyll was not correlated to Vitamin C content. Total protein contents of cotyledon was not changed significantly under light irradiation. But the soybean sprouts grown under different quality of light, hypocotyl was higher than those grown darkness. (37% and 20% higher for blue light and white light) Densitometric tracing of disc polyacrylamide gel electrophoretic patterns showed that protein of hypocotyl under white light had more high molecular weight protein.

• 한국식품조리과학회지
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• 제14권5호
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• pp.509-512
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• 1998

본 연구는 콩이 발효에 의해 메주, 된장으로 가공되면서 장내 가스 발생 인자인 stachyose, raffinose의 함량 변화와 $\alpha$-galactosidase의 활성 변화를 살펴보기 위하여 수행되었다. Stachyose, raffinose는 각각 콩 31.8239 mg/g, 2.6914 mg/g, 메주 4.2217 mg/g, 1.7413 mg/g, 숙성 된장 2.1184mg/g, trace로 나타나 대두 발효 과정에 따라 감소하였다. $\alpha$-Galactosidase 활성은 콩 14.5954 units/mg protein, 메주 13.1489 units/mg protein으로 차이가 적었으나 된장에서는 1.9157 units/mg protein으로 나타나 현격한 감소 양상을 보였다.

• Journal of Korean Biological Nursing Science
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• 제10권1호
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• pp.1-10
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• 2008

Purpose: The purpose of this study was to investigate the cerebral infarction size, antioxidant enzyme activities and lipid peroxidation changes after 6 weeks of dietary soybean protein intake in a rat focal brain ischemia model. Method: Weaning Sprague-Dawley rats were fed with either modified AIN-93G diet containing casein 20% (control), 20% soybean protein isolate-based diet (S20), or 40% of soybean protein isolate-based diet (S40) for 6 weeks. The animals were subject to right middle cerebral artery occlusion for 2 hr. After 24 hr of recirculation, the rats were sacrificed. Antioxidant enzymes activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and thiobarbituric acid reactive substance (TBARS) level in the right brain were also measured. Result: There were no significant differences in the right cortical infarction volume, TBARS level, SOD and CAT activities among the three groups whereas the GPx activities of the S20 group were significantly higher than those of the control group (p=.02). Conclusion: Our results suggest that 20% of soybean protein may have a modulating effect on GPx and possibly have some protective effect against oxidative stress although it may enough to decrease cerebral infarction volume in rat focal brain ischemia model.

• 한국식품영양과학회지
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• 제12권4호
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• pp.368-373
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• 1983

제사공장(製絲工場)에서 부산물(副産物)로 나오는 번데기 단백질(蛋白質) 영양가(營養價)를 측정(測定)하기 위하여 이의 아미노산조성을 분석하였고 casein군(群)($D_1$), 대두단백질군(大豆蛋白質群)($D_2$), 대두단백질(大豆蛋白質)+20%번데기단백질군(蛋白質群)($D_3$), 대두단백질(大豆蛋白質)+40%번데기단백질군(蛋白質群)($D_4$), 번데기단백질군(蛋白質群)($D_5$)등(等) 5군(群)의 식이(食餌)로 나누어 동물실험(動物實驗)을 통(通)해 성장률(成長率), protein efficiency ratio, 장기중량(臟器重量), 그리고 hematology 및 total serum protein과 albumin의 함량을 측정(測定)하여 비교(比較)하였다. 번데기단백질(蛋白質)의 아미노산조성은 FAO의 provisional scoring pattern과 비교(比較)하였을 때 필수아미노산 조성이 우수하였으며 특히 lysine과 methionine의 함량이 높은 것은 곡류(穀類)와 두류(豆類) 단백질(蛋白質)에 부족(不足)한 필수아미노산을 보충(補充)하는데 좋은 단백질원(蛋白質源)으로 이용(利用)될 수 있다. 번데기단백질군(蛋白質群)의 체중증가량(體重增加量)과 단백효율(蛋白效率)은 대두단백질(大豆蛋白質)보다 우수하였으며 대두단백질(大豆蛋白質)에 첨가하는 양(量)의 증가(增加)에 따라 성장율과 단백효율(蛋白效率)이 증가(增加)하는 경향을 나타내었다. liver와 spleen의 총 중량은 대두단백질군(大豆蛋白質群)에 비(比)해 번데기단백질(蛋白質) 첨가군(群)에 있어서 더욱 높았으며 RBC, WBC, Hct과 Hb함량(含量)은 5식이군(食餌群) 모두 정상치(定常値)에 속(屬)하였다. Total serum protein과 albumin의 함량(含量)은 번데기단백질(蛋白質)의 첨가로 인해 현저히 증가(增加)하는 결과를 보여주고 있다. 이상의 결과로서 번데기단백질(蛋白質)은 대두단백질(大豆蛋白質)의 질(質)을 향상(向上)시킬 수 있는 단백질원(蛋白質源)으로 사료(思料)된다.

• 한국작물학회지
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• 제46권2호
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• pp.106-111
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• 2001

The applicability of near infrared reflectance spectroscopy(NIRS) was tested to determine the protein and oil contents in ground soybean [Glycine max (L.) Merr.] seeds. A total of 189 soybean calibration samples and 103 validation samples were used for NIRS equation development and validation, respectively. In the NIRS equation of protein, the most accurate equation was obtained at 2, 8, 6, 1(2nd derivative, 8 nm gap, 6 points smoothing and 1 point second smoothing) math treatment condition with SNV-D (Standard Normal Variate and Detrend) scatter correction method and entire spectrum by using MPLS (Modified Partial Least Squares) regression. In the case of oil, the best equation was obtained at 1, 4, 4, 1 condition with SNV-D scatter correction method and near infrared (1100-2500nm) region by using MPLS regression. Validation of these NIRS equations showed very low bias (protein:-0.016%, oil : -0.011 %) and standard error of prediction (SEP, protein: 0.437%, oil: 0.377%) and very high coefficient of determination ($R^2$, protein: 0.985, oil : 0.965). Therefore, these NIRS equation seems reliable for determining the protein and oil content, and NIRS method could be used as a mass screening method of soybean seed.

• 한국의류산업학회지
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• 제16권3호
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• pp.462-468
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• 2014

This study investigates the dyeability of soybean protein fabric after dyeing with gallut. In this study, the colorants of gallnut were extracted with boiling water at $60^{\circ}C$ and 60min. Soybean protein fabric was dyed with extracted solution from gallnut according to concentration, temperature and time. After dyeing with gallut solution, dyeability (K/S) was obtained by CCM observation. Al, Cu and Fe were employed as a mordanting agents. Various color changing were produced by the interactions of mordants that were different from soybean protein fabric; however, Al mordant was not indicated as a variable color change. Fastness to washing and light were also investigated. Consequently, the dyeability of the soybean protein fabric dyed with gallut showed reasonable results at 100% o.w.f, $90^{\circ}C$, for 60min. Fastness to washing according to concentration indicated good result as more than grade (4-5) in general. Fastness to light was almost the same compared to fastness to washing at all concentrations. Fastness to washing according to mordants indicated good grade (4) in principle at Al, Cu, and Fe; in addition, fastness to light indicated a good grade too. Fastness to light also indicated the highest dyeability at Fe mordant. This study shows that gallut is a reasonable dyestuff to soybean protein fabric and that it is possible to manufacture a variety of products that use soybean protein fabric.