• Title/Summary/Keyword: soybean germplasm

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Variance of Agronomical Quantitative Traits in Mung Bean (Vigna radiata (L.) R. Wilczek var. radiata) Germplasm

  • Hyemyeong Yoon;Yu-Mi Choi;Kebede Taye Desta;Sukyeung Lee;Myong-Jae Shin;Xiaohan Wang;Joungyun Yi;Young-ah Jeon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.31-31
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    • 2023
  • Mung bean(Vigna radiata (L.) R. Wilczek var. radiata) is a legume that originated in India. It is the third most cultivated legume in Korea after soybean and adzuki bean. Recently, the use of mung bean seeds and sprouts in trendy foods such as rice noodles and Chinese-style stir-fry is expanding thereby increasing its demand. Subsequently, improvement of mung bean varieties is also being actively conducted. In this study, the important agricultural characteristics of 324 mung bean germplasm were recorded and statistically investigated. Seeds of the mung bean germplasm were cultivated at an experimental field located in the National Agrobiodiversity Center (Jeonju, Korea) and 10 quantitative agricultural traits were investigated. Basic statistics, correlation analysis, and principal component analysis were then performed. The results showed significant variations of the quantitative traits among the germplasms (p < 0.05). The days to flowering, maturity, and growth were in the ranges of 31~80, 22~72, and 57~110 days with means of 45, 47, and 92 days, respectively. The highest frequency (f = 192) was for lodging score with 11~50%, while simultaneous maturity (f = 182) was below 50%. Other quantitative traits related to yield including the number of seeds per pod (CV = 10.9%), number of pods per plant (CV = 41.2%), and one-hundred seeds weight (CV = 36.6%) also showed significant variations. Correlation analysis showed positive correlations between the days to maturity and one-hundred seeds weight (r = 0.41) and the days to growth and simultaneous maturity (r = 0.39). In contrast, one-hundred seeds weight was negatively correlated to the number of pods per plant (r = -0.41) and the days to flowering (r = -0.29). Similarly, the days to growth and the number of pods per plant had a negative association with each other (r = -0.29). The principal component analysis revealed the number of days to maturity as the most influential variable along the first principal component (23.7%). In general, this study revealed wide variations in quantitative traits among the studied mung bean germplasm, which could provide several options for cultivar development.

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Changes of Germination Rate of Pulses Seed Germplasm after Long-term Conservation

  • Baek, Hyung-jin;Lee, Young-yi;Jung, Yeon-ju;Yoon, Mun-seop
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.44-44
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    • 2018
  • The seeds of soybean (Glycine max), adzuki bean (Vigna angularis), mung bean (Vigna radiata), and kidney bean (Phaseolus vulgaris L.) were examined the germination rate after 10 years of long-term storage ($-18^{\circ}C$) conservation. For soybean seeds, 2,313 accessions were examined and germination rate of 1,082 accessions was decreased with below 15% of initial germination rate. For 227 accessions of soybean, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 589 accessions was increased and showed no change for 415 accessions after 10 years of long-term storage. For adzuki bean seeds, 2,058 accessions were examined and germination rate of 739 accessions was decreased with below 15% of initial germination rate. For 63 accessions of adzuki bean, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 535 accessions was increased and showed no change for 721 accessions after 10 years of long-term storage. For mung bean seeds, 438 accessions were examined and germination rate of 139 accessions was decreased with below 15% of initial germination rate. For 5 accessions of mung bean, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 155 accessions was increased and showed no change for 139 accessions after 10 years of long-term storage. For kdney bean seeds, 366 accessions were examined and germination rate of 7 accessions was decreased with below 15% of initial germination rate. For 65 accessions of kidney bean, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 201 accessions was increased and showed no change for 93 accessions after 10 years of long-term storage.

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Screening for Genotypes Lacking Lipoxygenase from Germplasm Collection of Korean Soybean Land Races (한국 재래종 콩집단에서 비린내 없는 콩품종 육성을 위한 Lipoxygenase 결실인자 변이 연구)

  • Kwon, Shin-Han;Park, Kyung-Sook;Kim, Mi-Young;Kim, Bong-Ryong;Song, Hi-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.6
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    • pp.528-533
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    • 1992
  • Soybean seeds contain lipoxygenase, which is responsible for the objectionable beany flavors in soybean seeds. The isozymes of lipoxygenase (1$\times$1, 1$\times$2, 1$\times$3) were discovered in United States of America, Japan, and Korea, and the mode of inheritance of the mutant genes was determined. This investigation was conducted to screen lipoxygenase-1, 2, and 3 lacking soybean lines from the Korean soybean land race population. Two lipoxygenase-1lacking lines, KAS 610-8 and KAS 621-8 were found in this investigation. In general, lipoxygenase acking varieties were small in seed size and low in oil content. A severe pod borer damage was observed in the two selected lipoxygenase-1 lacking lines. Lipoxygenase lacking line was not found in Korean wild soybean population used in this study and the lipoxygenase lacking lines were found only in Kyung-Nam province and the results imply that lipoxygenase lacking mutants were induced recently in cultivars.

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Genome Wide Association Study for Phytophthora sojae Resistance with the Two Races Collected from Main Soybean Production Area in Korea with 210 Soybean Natural Population

  • Beom-Kyu Kang;Su-Vin Heo;Ji-Hee Park;Jeong-Hyun Seo;Man-Soo Choi;Jun-Hoi Kim;Jae-Bok Hwang;Ji-Yeon Ko;Yun-Woo Jang;Young-Nam Yun;Choon-Song Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.202-202
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    • 2022
  • Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r2=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.

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Relationship between Seed Weight and Agronomic Traits in Korean Native Soybeans (재래종 대두의 종실중과 저형질과의 관계)

  • Song, Hi-Sup;Lee, Young-Il;Kwon, Shin-Han;Song, In-Man;Kwon, Kyeong-Hak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.28-33
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    • 1990
  • The present study was conducted to evaluate agronomic characters of the soybean germplasm which was collected from the sites covering southern parts of Korean peninsula. The total collections of 2748 native soybean varieties were grouped by eight levels of seed weight and the correlation between the seed weight and the other agronomic traits were determined. Seed weight was ranged from 7. 3g to 48. 4g with a great variation and averaged 23. 5g. Seed size of the many local lines were relatively large than that of foreign varieties. Frequency distribution of the germplasm for seed weight were 2.2% in Group 1 (below 10.0g), 14.7% in Group 2 (10.1-15.0g), 11.7% in Group 3 (15.1-20.0g), 26.2% in Group 4 (20.1-25.0g), 30.2% in Group 5 (25.1-30.0g). 10.6% in Group 6 (30.1-35.0g), 3.3% in Group 7 (35.1-40.0g) and 1.1% in Group 8 (over 40.0g). There was positive correlationship between seed weight and maturity or fruiting period, while there was negative correlationship between the seed weight and the other traits such as days to flowering, plant height, and number of nodes and pods. While 100 seed weight of 464 lines (16.9% of total lines) were below 15g, and those are adequate for the bean sprouts. The fruiting period of large seeded lines was longer, however the number of pods per plant of those were less lines than that of small seeded lines.

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Ecological Characteristics of Color - Soybean Collections (유색콩 수집종의 주요 생태적 특성)

  • 정찬식;백인열;고미석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.2
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    • pp.173-177
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    • 1996
  • This study was carried out to improve utility value as soybean genetic resources and to obtain basic information for color-soybean breeding. The 146 local color soybean lines collected in Kyongbuk province were investigated major agronomic characteristics. Seed coat-color showed seven different types which were black, white mottled on black, white mottled on brown, black saddle on green, green, raddish brown and brown. Major color types were black and green colors. The 90 percent of collected lines were purple in flower color. The 86 lines were green in color of cotyledonary part and 64 lines yellow. Great variations were observed in days to flowering and days from flowering to maturity, 51 to 81 days and 75 to 103 days, respectively. But days to maturity was 150 to 159 days and had smaller variation than days to flowering and days from flowering to maturity in all lines except those with white mottled on black (123 days). The 100 seed weight ranged below 10g to over 36g. Black seed color with green embryo seed was heavier seed weight than black seed color with yellow embryo seed. Seed shape of all collected lines was ellipsodial and average ratio of length: width: thickness of seed was 1:0.87:0.69. Positive correlation coeffieients were obtained among seed length, width, thickness and 100 seed weight.

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Variation of Seed Component Contents in Wild Soybean(Glycine soja Sieb. & Zucc.) (야생콩(Glycine soja Sieb. & Zucc.)의 종실성분 함량변이)

  • Yun Hong-Tae;Seo Min-Jung;Kim Sun-Lim;An Sun-Ok;Kim Si-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.108-111
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    • 2005
  • Studies were conducted to develop new breeding resources using wild soybean (Glycine soja Sieb. & Zucc.) species. In 132 wild soybeans, the average crude protein content was $45.4\%$, ranged from $37.4\%\;to\;50.2\%$ and average crude oil content was $9.3\%$ showing lower than cultivated soybean. The average total amino-acids content was $19,214\;{\mu}g/g$, containing $252\;{\mu}g/g$ of methionine and $103\;{\mu}g/g$ of cysteine, a sulfur containing amino-acid. In ratio of individual amino-acids, the glutamic acid, methionine and cystein content was $16.1\%,\;1.3\%,\;and\;0.5\%$, respectively. The correlation between total amino-acid and sulfur containing amino-acid was not significant. Total isoflavone concentration was, on average, $1,105\;{\mu}g/g$ in wild soybean germplasm. As a result of comparison with total isoflavone and genestein contents, significant positive correlations were observed between total isoflavone and genistein concentration.

Distribution of Anthocyanin Contents According to Growth Stages in Black-seeded Soybean Germplasms (검정콩 유전자원의 생육기별 안토시아닌 함량 분포)

  • Lee, Joo-Young;Hwang, In-Taek;Choi, Byung-Ryul;Yi, Eun-Seob;Kim, Yong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.3
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    • pp.338-342
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    • 2015
  • There has been known C3G (Cyanidin-3-Glucoside), D3G (Delphinidin-3-Glucoside), and Pt3G (Petunidin-3-Glucoside) were main anthocyanin pigments in black-seeded soybean. Anthocyanin contents of total 1,032 black-seeded soybean germplasms were analyzed by HPLC. Average of total anthocyanin content was 11.67 mg/g on the all materials ranged from 0.54 to 23.45 mg/g. Mean value of C3G, D3G, and Pt3G contents in all black-seeded soybeans were 8.81, 1.78 and 0.79 mg/g, respectively. Environmental conditions influenced anthocyanin contents during seed development. Delayed flowering, especially of later maturing germplasms, has been showed to result in increased anthocyanin content. So, prolonged maturation period germplasm is generally higher than that of shorten genotypes. It may be concluded that the higher levels of anthocyanin content was associated with the late dates of harvest maturity. Also larger seeds showed high anthocyanin contents than smaller. That inclination is similar in C3G's occasion because C3G content contribute highly to total anthocyanin content than other pigments.