• Title/Summary/Keyword: soybean cultivation

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Effects of Temperature on the Development and Reproduction of Matsumuraeses falcana (Lepidoptera: Tortricidae) (어리팥나방(Matsumuraeses falcana)의 발육과 생식에 미치는 온도의 영향)

  • Jeong Joon, Ahn;Eun Young, Kim;Bo Yoon, Seo; Jin Kyo, Jung
    • Korean journal of applied entomology
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    • v.61 no.3
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    • pp.435-447
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    • 2022
  • The soybean podborer, Matsumuraeses falcana (Lepidoptera: Tortricidae), is one of important pests in soybean crop. In the purpose of forecasting population dynamics of M. falcana, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of Matsumuraeses falcana at seven constant temperatures of 10, 13, 19, 22, 25, 28, and 31℃. Eggs hatched successfully at all temperature subjected. M. falcana developed from egg hatching to adult emergence at the tested temperatures except 10, 13, and 31℃. The developmental period of each life stage and adult longevity of M. falcana decreased as temperature increased. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of M. falcana were estimated by linear regression as 10.2℃ and 492.04DD, respectively. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. TL and TH from egg hatching to adult emergence using SSI model were 16.7℃ and 29.1℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of M. falcana was 12.4℃. We constructed the adult oviposition model of M. falcana using adult survivorship and fecundity. Temperature-dependent immature development and adult oviposition models will help constructing the population model of M. falcana and developing the strategies of integrated pest management in soybean fields.

Fed-batch cultivation for cell growth and spore production by probiotic B. polyfermenticus SCD

  • Park, Gyu-Yong;Lee, Gwang-Ho;Kim, Seong-Mi;Kim, Won-Seok;Baek, Hyeon-Dong
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.390-393
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    • 2001
  • The optimal temperature, pH and aeration rate for spore production by Bacillus polyfermenticus SCD in 500 ml shake flask and 5-1 jar fermenter were found to be $32^{\circ}C$, 7.0 and 1.0 vvm. respectively. When batch culture processes was performed under optimized culture conditions. viable cells were $3.3{\times}10^{10}$ CFU/ml and spore cells were $3.3{\times}10^{10}$ CFU/ml. Fed-batch culture processes were also examined with regard to higer maximum viable cell and spore production. The highe viable cells and spores were obtained in 5-1 jar fermenter at 72 h cultivation time by strategy in an intermediate feeding mode with 60% glucose solution 150 ml and 5% soybean flour solution 150 ml fed to the fermenter twice, and the productivity of spore cells was significantly increased. Finally. volumetric productivity of spore cells on fed-batch culture indicated $9.9{\times}10^8$ CFU/ml/h, which was approximately 2 times higher than batch culture. Thus, fed-batch culture show a promise as an industrial production method.

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Production of Toxin Protein by Recombinant Escherichia coli with a Thermally Inducible Expression System

  • Jong, Se-Han;Chang, Ho-Nam;Chang, Yong-Keun;Rhim, Seong-Lyul
    • Journal of Microbiology and Biotechnology
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    • v.6 no.6
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    • pp.451-455
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    • 1996
  • Physiological studies on the expression of Bacillus thuringiensis subsp. tenebrionis (Btt) gene coding for insecticidal protein in recombinant Escherichia coli 537 were carried out to identify optimal culture condition. It was necessary to shift culture temperature from 30 to $42^{\circ}C$ to express the gene. Expression of the Btt toxin gene by recombinant E. coli 537 began within one hour after induction. Complex nitrogen sources increased production of the insecticidal protein. The total insecticidal protein was 0.5 g/I when using yeast extract as a complex nitrogen source. Soybean hydrolysate showed apparently the highest induction efficiency. After induction, the cellular content of the insecticidal protein was 5.4 times higher than it had been before induction. The optimal cultivation strategy was found to grow cells for 7hours at $30^{\circ}C$ and then 5-8 hours at $42^{\circ}C$. The optimal cultivation pH for the production of insecticidal protein was 6.5. The Btt toxin produced by the recombinant E. coli 537 was found to have the same level of potency against Colorado potato beetle as the original toxin.

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Isolation of Keratinolytic Protease Producing Microorganism and Its Cultivation Condition (Keratinolytic protease 생산균, Pseudomonas sp. KP-364의 분리 및 배양)

  • 전동호;권태종
    • Microbiology and Biotechnology Letters
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    • v.29 no.3
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    • pp.134-141
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    • 2001
  • A bacterial strain KP-364 producing extracellular keratinolytic protease was isolated from the soil of the poultry fac-tory. It was identified as Pseudomonas sp. based on its morphological and physiological characteristics, The optimal culture conditions for the production of keratinolytic protease by Pseudomonas sp. KP-364 were investigated. The composition of optimal medium for the keratinolytic protease was 2.0% glucose, 0.5% soybean meal. 0.5% $NaNO_3$ and 0.2% KCI Optimal initial pH for production of Keratinolytic protease production were 6.5 and $37^{\circ}C$ respec- tively. The keratinolytic protease production reached a maximum of 1,270 U/ml/hr after 48 hours cultivation under the optimal culture conditions.

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Cultural Condition for the Mycelial Growth of Phellinus igniarius on Chemically Defined Medium and Grains (화학합성배지 및 곡물을 이용한 Phellinus igniarius의 균사체 배양조건)

  • Jung, In-Chang;Kim, Seon-Hee;Kwon, Yong-Il;Kim, So-Yeun;Lee, Jong-Suk;Park, Shin;Park, Kyung-Sook;Lee, Jae-Sung
    • The Korean Journal of Mycology
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    • v.25 no.2 s.81
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    • pp.133-142
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    • 1997
  • The chemical media composition and culture conditions were optimized for mycelial growth of Phellinus igniarius 26005. The method of solid-state fermentation, cultivation of basidiomycetal strains in various grains, was developed. Media composition for optimal growth of Phellinus igniarius 26005 was made of 7.0% malt extract, 0.3% bacto soytone, and 0.2% yeast extract. The optimum condition for mycelial growth was $28^{\circ}C$ and pH 7.0, respectively. For the mass cultivation of mycelia, the hydrated grains with cold water, were put into the plastic bottle. The mycelial growth rate in the bottled grains was high in the early stage with inoculation of homogenized mycelium. The activity of mycelium was maintained by adding sterilized water in the middle of cultivation. The glucosamine content which determins the mycelial growth rate in solid material was in the order of job's tears>barley>black soybean>wheat>malt soybean>brown rice>sorghum>glutinous rice.

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2 Cropping systems using field crops in unheated plastic house at paddy field

  • Shin, Jung-Ho;Moon, Jin-Young;Song, Jae-Ki;Choi, Yong-Jo;Hong, Kwang-Pyo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.279-279
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    • 2017
  • In Korea, the single span unheated plastic house cultivated crops from autumn to spring of the following year, removed the plastic film and frame, cultivated rice, set up a plastic house again and cultivated crops. The crops in the greenhouse are utilized mainly for the production of leaf vegetables such as lettuce, leek, and fruit vegetables such as strawberry, watermelon, oriental melon, etc. and raising high income. Because, the production of these crops has characteristics requiring a lot of labor and it is difficult to produce horticultural crops at unheated plastic houses as the rural population ages. Therefore, we conducted a test to develop a crop planting system to cultivate crops in single span unheated plastic houses, although the utilization of labor is less than that of horticultural crops. The prior cropping cultivated three cultivars of sweet potatoes early, the second produced cultivated sweet potatoes, corn and soybeans. In the cultivation of the previous cropping, the sweet potatoes were harvested on the 113th day after planting on March 30th, the yield was 822 kg/10a for Pungwonmi, 1,377 kg/10a for Jinhongmi, 1,483 kg/10a for the Dahomi. Because of differences, the yield of Pungwonmi cultivar was less than that other cultivars and the yield of open field cultivations, we will expect further research. In the cultivation of the succeeding crops sweet potatoes were planted on July 27 and harvested 110 days later and investigated. The product yield of Pungwonmi cultivar was 1,024 kg/10a, and the Jinhongmi, Dahomi cultivars were not at economic level for sale and were necessary to review. In succeeding-crops, corn tested the Ilmichal cultivar, seeded on 27th July, harvested on October 11th. The day of silking was 45 days after sowing, the yield was 1,156 kg/10a, the goods rate was 100% level. The beans in the succeeding cultivation crop were sowed on 27th July, the early maturing of the varieties coming to Hwangeumol and Saeol cultivar, on 17th October, the late maturing soybean Daewonkong cultivar were harvested on October 21st. The yield of early maturing two cultivars was 214 kg/10a, Daewonkong was 257 kg/10a, and 100 seeds weight which were more than the early maturing beans were also heavy. When calculating these incomes price-wise according to the harvest time, we were able to consider the income in the order of corn, sweet potato and soybean from the second term crop. Various studies such as varieties, mulching method, moisture management, control environment management, etc. are considered necessary to develop cropping systems with sweet potato and field crops in future unheated plastic house.

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Relationship between Number of Soil Micro-organisms and Change of Cropping System (답전(畓田) 윤환시(輪換時) 작부체계(作付體系)와 토양미생물상(土壤微生物相) 변화(變化)와의 관계(關係))

  • Lee, Sang-Kyu;Yun, Sei-Young;Kim, Seung-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.1
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    • pp.70-76
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    • 1992
  • A field experiment was conducted to find out the influence of cropping systems under rotation of paddy-upland soil on soil microorganisms with specific reference to cations concentration in the soil. The results obtained was summarized as follows. 1. The number of soil bacteria and actinomycetes increased in fallow, continuous cultivation of rice and soybean while the number of fungi decreased. 2. Gram negative bacteria as Pseudomonas spp. and Rhizobium spp. remarkably incerased with increasing Gram positive bacteria of Bacillus subtilis in continuous cultivatio of soybean. 3. The relative population of soil born plant pathogen such as Fusarium spp. Rhizoctionia spp. and Phoma spp. to the total soil fungi was high in cultivation of potato and Chinese cabbage. The ratio of soil plant pathogen to the total soil fungi was high in cultivation of potato with Chinese cabbage. 4. The number of bacteria and actinomycetes was positively correlated with ratio of Ca+Mg/K in soil while negatively correlated with soil fungi.

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Studies on the optimal conditions for the high yield in the production of Ganoderma lucidum (영지버섯 다수확 생산을 위한 최적조건에 관한 연구)

  • Kim, Hong-Kyu;Kim, Yong-Kyun;Seo, Gwan-Seuk;Oh, Se-Hyeon;Kim, Hong-Gi
    • Journal of Mushroom
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    • v.3 no.4
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    • pp.154-158
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    • 2005
  • The results were as follows; 3 additives(rice bran, wheat bran and soybean bran) to the Quercus dentata sawdust were applied at 10, 20 and 30%. High yield was recorded at 20% of rice bran and wheat bran and 30% of soybean bran. Among various sawdust media, the cultivation with the Quercus dentata sawdust alone showed the most favored growth index, such as 22 days of full mycelial growth, 11 days of pinheading from spawning and 23.5g per $1,200m{\ell}$ bottle. In the size of bottles, the lowest infection ratio was 5 % at $1,200m{\ell}$ bottle. In the growth characteristics with the bottle size, Reishi at $1,600m{\ell}$ bottle showed best result when it is observed for 13~15 days in pinheading from spawing, 36~37 days in fruit bodies growing and 26.7~29.3g yield per bottle. The highest yield per area was 6.4kg at horizontal bed with 218 bottles per $3.3m^2$. The Reishi produced in $1,600m{\ell}$ bottle with horizontal cultivation had less number of fruit bodies and best yield, thus it is determined to be the most economic method.

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Analysis of Soil Microbial Communities Formed by Different Upland Fields in Gyeongnam Province

  • Kim, Min Keun;Ok, Yong Sik;Heo, Jae-Young;Choi, Si-Lim;Lee, Sang-Dae;Shin, Hyun-Yul;Kim, Je-Hong;Kim, Hye Ran;Lee, Young Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.2
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    • pp.100-106
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    • 2014
  • The present study investigated variations in soil microbial communities by fatty acid methyl ester (FAME) and the chemical properties at 24 sites of upland soils in Gyeongnam Province. The electrical conductivity of the soil under potato cultivation was significantly higher than those of the red pepper and soybean soils (p < 0.05). The gram-negative bacteria community in potato soil was significantly lower than those in the garlic and soybean soils (p < 0.05). The communities of actinomycetes and arbuscular mycorrhizal fungi in the red pepper soil were significantly higher than those in the potato soil (p < 0.05). In addition, the cy17:0 to 16:$1{\omega}7c$ ratio was significantly lower in red pepper, soybean, and garlic soils compared with potato soil, indicating that microbial stress decreased. Consequently, differences in soil microbial community were highly associated with cultivated crop species, and this might be resulted from the difference in soil chemical properties.

Nutrient Balances and Soil Properties Affected by Application of Soybean and Barley Residues

  • Oh, Taek-Keun;Sonn, Yeon-Kyu;Lee, Dong Sung;Kim, Myung-Sook;Kim, Seok-Cheol;Yun, Hong Bae;Lee, Deog-Bae;Lee, Chang-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.2
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    • pp.120-126
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    • 2014
  • An accurate analysis of nutrient balance in different cropping systems is necessary for improving soil fertilities, causing higher crop yields and quality. This study was carried out to investigate the nutrient balance, changes in soil properties, and their effects on crop yield in long-term field cultivation under mono- and rotation-cropping systems (MCS and RCS, respectively). The analytical results of the soil properties showed that the application of mineral fertilizers alone in the MCS leads the reduction of soil CEC, exchangeable Ca, and microbial biomass C and N. Compared with the MCS of soybean, the RCS of soybean and barley significantly improved the soil properties, which increased crop yield. It might be due to the barley residue added to the RCS soil. Mean nutrient balances for 4 years were -55.9 kg N, +34.7 kg $P_2O_5$, and -0.3 kg $K_2O$ $ha^{-1}$ for the MCS and +19.7 kg N, +107.4 kg $P_2O_5$, and -48.6 kg $K_2O$ $ha^{-1}$ for the RCS, respectively. These nutrient imbalances mean that conventional fertilizer recommendations were inadequate for maintaining soil nutrient balance. From these results, we can conclude that the crop rotation may change comprehensive physical, chemical, and biological soil properties. These changes could affect the nutrient balance and then the crop yield.