• Korean Journal of Weed Science
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• v.4 no.2
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• pp.194-203
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• 1984

To evaluate the compatibility in systemic use of 4 kinds of herbicides on both weeds and soybeans, trials were conducted at Coll. Exp. Farm/Jonnam Nat'l. Univ., 1983. Among the experimented 4 herbicides, alachlor or metribuzin were applied at pre-emergence, and followed by postemergence spraying of acifluorfen or bentazon, respectively. All herbicides were applicated at rates of 0, 0.25, 0.5, and 0.75 kg active ingredients per ha. Under the conditions which Echinochloa crusgalli and Digitaria adscendense were dominating, most comprehensive compatibility was found from each rates 0.5 - 0.75 kg/ha at 60 days after crop seeding date. However, slight transient leaf burn was evident at the plots of metribuzin and/or acifluorfen applicated. The positive tendency of herbicide interactions in weeding efficacy was observed from the system in alachlor sequence. However, the results in crop growth and yields at hervest indicate the necessity of higher rates of each herbicides upto 0.75 kg/ha for the consistence weeding efficacies. And more excellent weeding results (crop growth and yields) on crop plants were provided from the plots which applicated by residual type herbicide, namely acifluorfen than bentzaon.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.427-431
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• 2002

Lateral roots of soybean sprout might reduce the quality. The study was done to measure the effect of light quality treated during 24 hour imbibition or 6 day culture on growth and development of soybean sprouts on the 6th day after culture. With the soybean seeds imbibed in 4 ppm benzyladenopurine (BA) solution for last 6 hours of the imbibition, blue and red lights were treated during the imbibition, but during 6 day culture, blue and red or far-red light treatments were done for 50 minutes or 5 hours a day, respectively, the periods taking for their cotyledons to turn green color, On the 6th day after culture, the soybean sprouts were classified by 4 categories on the base of hypocotyl length;＞7cm, 4 to 7cm, ＜4cm and non-germination, and their lateral roots, hypocotyl diameters and fraction dry weights were measured. Blue and red lights treated during the imbibition completely blocked lateral root formation regardless of the lights treated during the culture, and showed nearly the same rate of hypocotyls of longer than 4cm. The period of each light treatment forced during the culture did not influence the growth of soybean sprouts. far-red light treated for 5 hours everyday, however, had the least rate of seed germination and hypocotyls of longer than 7cm of the light quality treatments. In addition, red and far-red lights almost equally having the commercial soybean sprouts of longer than 4cm hypocotyls move elongated and selenderized than blue light and dark treatment, meaning the growth and morphology of soybean sprouts was affected by light treatments during the culture.

• Journal of Plant Biology
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• v.17 no.4
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• pp.163-170
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• 1974

Leaf tissue of Glycine max Merr. was fixed in para-formaldehyde-glutarldehyde and postfixed in osmium tetroxide or postassium permanganate for electron microscopy. The origin of cytolysome-like organelles of mesophyll cell was studied and changes of fine structure of the organelles according to treating solutions such as gibberellin (GA), kinethin (KI), 2,4-dichlorophenoxy acetic acid(2, 4-D) or 2, 4-D＋GA(1mg/l, respectively) were observed. The cylolysome-like organelles differentiate in endoplasmic reticulum and plasmalemma, and they drop into vacuoles being isolated from the formers. They seem to change into myelin-like structure and to be degenerated by autodigestion. Cytolysome-like organelles involved in cell walls and vacuoles showed activity of acid phosphatase. In the group of GA and KI treatment, cytolysome-like organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D＋GA, myelin-like structures increased in size and autodigestion of this organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D＋GA, myelin-like structures increased in size and autodigestion of this organelle seemed to be accelerated. In the treatmental group of 2,4-D＋GA, myelin-like structures shown high electron density were observed in cytoplasm and vacuoles together.

• Korean Journal of Food Science and Technology
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• v.19 no.5
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• pp.441-445
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• 1987

The subcellular distribution of lipoxygenase in germinating soybean seeds (Glycine max[L.] AmSoy) was investigated by using differential centrifugation and sucrose density gradient fractionation. Most of lipoxygenase -1 and -2/3 activities was present in the supernatant fraction after differential centrifugation of homogenates prepared from three-day-old seedlings; only 1.5% of lipoxygenase activity remained in particulate fractions. The results of a sucrose density gradient fractionation (three-day-old) showed that the lipoxygenase activity coincided with acid phosphatase at the densities of 1.19, 1.23, $1.25g/cm^3$, even though most of lipoxygenase and acid phosphatase activities appeared in supernatant fractions. There was no indication that mitochondria contained any lipoxygenase activity, and it does not appear that glyoxysomes and ER contained any lipoxygenase activity either.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.629-632
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• 1999

Constructed wetlands are being used for the removal of nutrients from livestock wastewater. However, natural vegetation typically used in constructed wetlands does not have marketable value. As an alternative, agronomic plants grown under flooded or saturated soil conditions that promote denitrification can be used. Studies on constructed wetlands for swine wastewater were conducted in wetland cells that contained either natural wetland plants or a combination of soybeans and rice for two years with the objective of maximum nitrogen reduction to minimize the amount of land required for terminal treatment. Three systems, of two 3.6 by 33.5 m wetland cells connected in series were used; two systems each contained a different combination of emergent wetland vegetation: rush/bulrush (system 1) and bur-reed/cattail (system 2). The third system contained soybean (Glycine max) in saturated-soil-culture (SSC) in the first cell, and flooded rice (Oryza sativa) in the second cell. Nitrogen (N) loading rates of 3 and $10kg\;ha^{-1}\;day^{-1}$ were used in the first and second years, respectively. These loading rates were obtained by mixing swine lagoon liquid with fresh water before it was applied to the wetland. The nutrient removal efficiency was similar in the rush/bulrush, bur-reed/cattails and agronomic plant systems. Mean mass removal of N was 94 % at the loading rate of $3kg\;N\;ha^{-1}\;day^{-1}$ and decreased to 71% at the higher rate of $10kg\;N\;ha^{-1}\;day^{-1}$. The two years means for above-ground dry matter production for rush/bulrushes and bur-reed/cattails was l2 and $33Mg\;ha^{-1}$, respectively. Flooded rice yield was $4.5Mg\;ha^{-1}$ and soybean grown in saturation culture yielded $2.8Mg\;ha^{-1}$. Additionally, the performance of seven soybean cultivars using SSC in constructed wetlands with swine wastewater as the water source was evaluated for two years, The cultivar Young had the highest yield with 4.0 and $2.8Mg\;ha^{-1}$ in each year, This indicated that production of acceptable soybean yields in constructed wetlands seems feasible with SSC using swine lagoon liquid. Two microcosms studies were established to further investigate the management of constructed wetlands. In the first microcosm experiment, the effects of swine lagoon liquid on the growth of wetland plants at half (about 175 mg/l ammonia) and full strength (about 350 mg/l ammonia) was investigated. It was concluded that wetland plants can grow well in at least half strength lagoon liquid. In the second microcosm experiment, sequencing nitrification-wetland treatments was studied. When nitrified lagoon liquid was added in batch applications ($48kg\;N\;ha^{-1}\;day^{-1}$) to wetland microcosms the nitrogen removal rate was four to five times higher than when non-nitrified lagoon liquid was added. Wetland microcosms with plants were more effective than those with bare soil. These results suggest that vegetated wetlands with nitrification pretreatment are viable treatment systems for removal of large quantities of nitrogen from swine lagoon liquid.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.4
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• pp.325-330
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• 2004

This study was carried out to determine adequate planting date, to compare the growth characteristics between early and late maturing cultivars, and to provide the data for the cultivation techniques of soybean [Glycine max (L.) Merr.] in double cropping system with winter crops on paddy field in Korea. Cultivars were planted on 26 May, 16 June, and 7 July with a planting density of $70cm(row\;widtb)\;{\times}\;10cm$ (planting spacing). Seed yield of soybean planted on June 16 and July 7 was approximately $37\%\;and\;53\%$, respectively, less than that of conventional planting date of May 26 in Pungsan-namulkong, and planted on June 16 and July 7 was about $30\%\;and\;37\%$, respectively, less then that of conventional planting date of May 26 in Hanamkong. The number of pods and seeds per plant decreased as planting date delayed. Seed weight increased in Pungsan-namulkong but decreased in Hannamkong as planting date delayed. The flowering date was late in delayed planting plots, but it was shorted for days from emergence to flowering and from emergence to maturity. The plant height of Hannamkong was greater than Pungsan-namulkong from the emergence to flowering stages, but in contrast, it was greater in Pungsan-namulkong than Hannamkong after flowering stage (50d after emergence) when it planted on May 26. There were no significant differences between two soybean cultivars at planting dates of June 16 and July 7. Leaf number, leaf area, and dry matter were also reduced by late planting, and Both of them were shown in high reduction at the later planting. There was a high significant difference at the flowering $(r\;=\;0.87^{**})$ and pod formation $(r\;=\;0.91^{**})$ stages between leaf dry matter and seed yield. Crop growth rate (CGR) was greater at $R2\~R3$ growth stages compared to $R3\~R4\;or\;R4\~R5$ growth stages in two soybean cultivars and the greatest CGR was obtained at planting date of May 26 in two soybean cultivars except for R4-R5 growth stage in Pungsan-namulkong. There was a highly significant positive difference between the seed yield and the leaf area index (LAI) across R3 to R4 and R2 to R3 stages. The photosynthetic rate $(P_N)$ of the uppermost leaf position had no significant difference among planting dates and between two soybean cultivars. However, $P_N$ of the $7^{th}$ leaf position increased as the planting date delayed.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.29-33
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• 2010

Soybean (Glycine max (L.) Merr.) seed is the main source of protein and oil for human and animal. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are exist in the raw mature soybean. Lipoxygenase, Kunitz trypsin inhibitor (KTI) protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin are main antinutritional factors in soybean seed. Breeding of a new soybean strain with lacking these components is needed. The objective of this research was to select new soybean line with lipoxygenase-free, KTI-free, and ${\alpha}^{\prime}-subunit$ free (lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype). Total 434 $F_2$seeds were obtained from the cross of cultivar, "Gaechuck#2" and PI506876. Presence and absence of lipoxygenase, KTI protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin was tested by SDS electrophoresis using a partial seed of each $F_2$seed. Only one $F_2$seed with lacking these three components was selected and was planted to $F_2$plant. Absence of lipoxygenase, KTI, and ${\alpha}^{\prime}-subunit$ protein was confirmed on the $F_3$seeds harvested. Selected line with lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype might be useful for soybean breeding.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.101-101
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• 2020

Soybean is known as to have a several healthy ingredients. Among them, isoflavones are effective in reducing obesity, menopausal symptom. Isoflavones consist of 12 isomers, including Aglycon, Glucoside, Malonyl glucoside, Acetyl glucoside, and are usually found in soybean seeds. The content is determined by the sum of 12 isomers, and the content value difference between the varieties is huge. In this study, we investigated the agronomic traits, 12 isomer of isoflavone content and composition for 49 soybean germplasms. This germplasms were selected from the 23,000 germplasms with the highest total content of isoflavones possessed by the National Agrobiodiversity Center. Seed samples were cultivated in experimental field located in Jeonju City on April 04, 2019. Matured seeds were harvested and portions of each seed samples were oven-dried, pulverized, and analyzed for their isoflavone compositions using HPLC-DAD. The soybean samples showed distinction in their agronomic traits, isoflavone compositions and contents. The days to flowering ranged between 38 and 69 days while the days to maturity ranged between 103 and 156 days. The seed coat color of soybean germplasms was 24 in black, 10 in yellow, 2 in green, 5 in yellowish green, 4 in green with black spot, 4 in pale yellow. The germplasm with the highest total content of isoflavones was the IT178054(1257.61±7.98 ㎍/g), but the germplasms containing the largest number of isoflavone isomers were IT274592, IT275005, both germplasms had 11 isoflavone isomers excluding Malonyl glycitin. The largest source of Aglycon, the most easily absorbed isoflavone form in the human body, was IT274592(DZ: 8.83±0.30 ㎍/g, GL: 11.14±0.81 ㎍/g, GE: 8.16±0.26 ㎍/g), while only IT274592, IT275005, IT308619 contained all three components of Aglycon. In Principal Component Analysis(PCA), the first two principal components showed more than 3.5 Eigen value and accounted for 58.2% of variability. The total content value had strong relationship with Malonyl genistin content value. Acetyl isomers had strong relationship, but Malonyl isomers were only related to isomers except Malonyl glycitin. These results will help in research on soybean varieties to enhance isoflavone ingredients.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.220-227
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• 1997

Water deficit stress during early soybean[Glycine max (L.) Merrill] growth stage is the most important environmental factor limiting productivity. Eight soybean genotypes were grown in replicated pot under well-watered(control: near 0 bar) and drought(-5 and -10 bars) conditions. Soybean plants were subject to drought stress for 20 days at 10 days after seed emergence. Significant genotypic variation was observed for leaf area(LA) and total dry weight (TDW). At the end of water stress, LA and TDW of Hwanggeumkong and Paldalkong, which had large LA in the non-stressed control, were more sensitive to water stress than those of the other cultivars, while those of Suwon 93 with small LA were insensitive. Leaf proline and abscisic acid(ABA) contents increased after water stress. However, changes in proline and ABA contents were not consistently related to the changes in LA as affected by water stress. As the soil water potential decreased, the yield reduction of Hodgson 78 showing large decrease in LA and TDW in response to water deficit was severe when compared to that of Baegunkong with small decrease in LA and TDW. Relatively greater yield stability and higher average yield across soil water potential were observed in Baegunkong. Of specific interest was the small reduction in yield of Paldalkong in spite of its significant decrease in LA and TDW.

• Journal of Life Science
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• v.13 no.3
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• pp.280-290
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• 2003

The incorporation of RAPD markers into the previous classical and RFLP genetic linkage maps will facilitate the generation of a detailed genetic map by compensating for the lack of one type of marker in the region of interest. The objective of this paper was to present features we observed when we associated RAPD map from an intraspecific cross of a Glycine max$\times$G. max, 'Essex'$\times$PI 437654 with the public RFLP map developed from an interspecific cross of G. max$\times$G. soja. Among 27 linkage groups of RAPD map, eight linkage groups contained probe/enzyme combination RFLP markers, which allowed us the incorporation of RAPD markers into the public RFLP map. Map position rearrangement was observed. In incorporating L.G.C-3 into the public RFLP linkage group a1 and a2, both pSAC3 and pA136 region, and pA170/EcoRV and pB170/HindIII region were in opposite order, respectively. And, pk400 was localized 1.8 cM from pA96-1 and 8.4 cM from pB172 in the public RFLP map, but was localized 9.9 cM from i locus and 18.9 cM from pA85 in our study. A noticeable expansion of the map distances in the intraspecific cross of Essex and PI 437654 was also observed. Map distance between probes pA890 and pK493 in L.G.C-1 was 48.6 cM, but it was only 13.3 cM in the public RFLP map. The distances from the probe pB32-2 to pA670 and from pA670 to pA668 in L.G. C-2 were 50.9 cM and 31.7 cM, but they were 35.9 cM and 13.5 cM in the public RFLP map. The detection of duplicate loci from the same probe that were mapped on the same or/and different linkage group was another feature we observed.