• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.2
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• pp.85-94
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• 2002

Antibodies raised against the purified p-subunit of $\beta$-conglycinin were used in immunohistochemical studies to monitor the pattern of $\beta$-conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the $\beta$-subunit of $\beta$-conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the $\beta$-subunit of $\beta$-conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of $\beta$-conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the $\beta$-subunit of $\beta$-conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.

• Food Science and Biotechnology
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• v.15 no.3
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• pp.392-398
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• 2006

Four flavonoids 1-4 and one phytosterol 5 were isolated from methanol extract of Taekwangkong, one of the soybean cultivars, and the structures of these compounds were fully characterized by physical and spectral analysis. The content of compounds 1-4 as determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with diode-array detector were 12.1, 624.6, 18.0, and $219.6\;{\mu}g/g$, respectively, and the total phenolic content of this cultivar was measured as 3.7 mg gallic acid equivalent per g dry material (GAB/g). Also, compound 1 showed strong radical scavenging activity in the 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay ($IC_{50}\;=\;47.6\;{\mu}M$), five-fold higher than seen in the 1,1-diphenyl-2-picrylliydrazyl (DPPH) assay. These results lead to the conclusion that soybean not only has many phytoestrogens but also has potent antioxidant activity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.324-329
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• 1993

Effects of leaf and pod removal on changes in leaf and seed number, and leaf, seed, pod and stem dry weight of soybean [Glycine max (L,) Merr.] cultivar ‘Hwangkeumkong’ were measured at the research farm of Korea University in 1992. The upper 40% and lower 60% of leaves and pods were subjected to treatments at the growth stage of beginning pod(R3). Leaf number and dry weight of lower part were increased by upper leaf-lower pod removal, but seed number and dry weight were decreased. Upper leaf-lower pod removal increased lower stem dry weight and decreased upper pod dry weight. Leaf-seed ratio of the upper leaf-lower pod removal was the highest with 3.54 and harvest index was the lowest with 27% among the treatments. Lower leaf-upper pod removal showed that more assimilates from upper leaves were translocated to lower seeds than from lower leaves to upper seeds in upper leaf-lower pod removal treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.16-25
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• 1995

The effects of leaf removal time on variation of nodal sink components in determinated soybean ［Glycine max （L.） Merr.］ cultivar 'Danyeobkong' were measured at the experiment field of Chonbuk Provincial Rural Development Administration in 1991. Node order in this experiment was calculated from terminal node to bottom node to clarify the photosynthetic ability of canopy leaves. The upper 5 leaves from terminal node of main stem were removed at 5, 15, 25, 35 and 45 days after flowering(DAF) respectively. In spite of light-receiving increment in lower part of main stem and in branch by removing the upper part leaves of main stem, seed weight of that part was not increased in leaf removal treatment compared with control block. The node position reducing pod went down from terminal to bottom by delaying leaf removal time, and the influence of pod number reduction was bigger in DAF 25 than in DAF 15. But the seed number reduction per pod was the biggest in DAF 35. Cracked seed coat ratio ranged from 25% to 35% in control block, while that of leaf removal block had mostly 10% gap compared with the control block.rol block.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.377-382
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• 1993

Effects of leaf and pod removal on changes in dry weight and on the contents of soluble sugar, starch, protein and oil in leaves and seeds of soybean [Glycine max(L.) Merr.] cultivar ‘Hwangkeumkong’ were measured at the research farm of Korea University in 1992. The upper 40% and lower 60% of leaves and pods were subjected to treatments at the growth stage of beginning pod(R3). Upper leaf-lower pod removal showed the highest leaf and the lowest seed dry weights. Soluble sugar content was no different among treatments in leaves and seeds. The highest starch content was found in leaves of upper leaf-lower pod removal. Protein content was higher in lower leaves than upper leaves and the lowest in seeds of lower leaf-upper pod removal which had the highest oil content in leaves and seeds. These results apparently indicated that photoassimilates were mobilized from upper leaves to lower seeds, and protein sources were moved from lower to upper parts but weak in remobilization from leaves for the long distance translocation during the reproductive growth period.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.371-381
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• 1995

The effects of leaf and pod removal on variation of nodal sink components in determinate soybean [Glycine max (L.) Merr.] cultivar 'Danyeobkong' were measured at the experiment field of Chonbuk Provincial Rural Development Administration in 1991. Contrary to the conventional numbering system, node order in this experiment was numbered from top to bottom node. The leaves and pods of main stem from terminal to 5th node, below 6th node, or branches were removed at the growth stage of beginning pod(R3). In the leaf removal treatment, number and weight of pod and seed were highly decreased in upper part leaf removal, especially in removed part. In the pod removal treatment, number and weight of pod and seed were slightly increased in the other part. Cracked seed coat ratios were also high in the leaf removal treatment compared with control but low in the pod removal treatment.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.31-36
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• 2002

The patterns of adventitious root formation from cotyledons for each cultivar of soybeans were compared. The results of adventitious root formation in cultivars are classified as two groups; the first group showed the direct adventitious root formation, and the second group resulted in the callus and adventitious root formation. The cultivars that have much callus formation had less the adventitious root formation. The adventitious root formation in the cotyledonary explants was occured only at the inoculation of adaxial side. When adaxial and abaxial side was inoculated simultaneously, the adventitious roots were formed at the adaxial side. Thus, it suggests that there must be direction to some extent. Starch in the cotyledonary explants were more abundant at the 4 days after induction than at the early stage of the adventitious root formation, but the starch was not observed after 7 days, that the growth stage of adventitious roots.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.6-11
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• 1998

Bean sprout is one of the traditional vegetable in Korea and produced by sprouting soybean (Glycine max L.) cultivars. This experiments was conducted to investigate the ecological characteristics in collected small grain soybean lines from Chungnam province region. Thirty varieties were used in this study. Seed coat color of collected small soybean lines showed eight different types. Major seed coat color type was yellow as 8 lines of total 35 lines. The stem height, number of nodes and number of branches averaged 58.5 cm, 16.5 nodes and 11.8 branches, respectively. The average number of main stem pods and number of branching pod were appeared 43.1 and 62.7 respectively. One hundred seed weight averaged 9.9g which was lower than 10~12g of sprouting soybean cultivars as breeding goal of Korea. The average of node width showed 13.2cm and long stem open types and short stem close types were classified 1 line and 14 lines. The flower dates of collecting small soybean lines were between 8 Aug. and 13 Aug. and flowering duration was 74~85 days. The date of maturity were between 12 Sep. and 12 Oct., and maturity duration showed great variation among cultivated soybean lines.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.278-286
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• 2021

Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.2
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• pp.81-88
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• 2003

Soybeans [Glycine max (L.) Merr.] are frequently exposed to unfavorable environments during growing seasons and water is the most important factor limiting for the production system. The purpose of this study was to determine the leaf water potential changes by irrigation, and to evaluate the relationships of leaf water potential, growth and yield in soybeans. Three soybean cultivars, Hwangkeumkong, Shinpaldalkong 2, and Pungsannamulkong, were planted in growth chamber and field with irrigated treatments. Leaf water potential of three soybean cultivars was positively correlated with leaf water content during vegetative and reproductive growth stages in growth chamber and field experiments. Leaf water potentials measured for three soybean cultivars under growth chamber were higher than those of under field conditions. Higher leaf water potential with irrigated plots under field was observed compared to conventional plots during reproductive growth stages. Leaf water potentials of three soybean cultivars were continually decreased during reproductive growth stages under field and there was no significant difference among them. Number of leaves, leaf water content, pod dry weight, number of seeds and seed dry weight with irrigated plots were higher than those of conventional plots. The results of this study suggested that leaf water potential could be used as an important growth indicator during the growing season of soybean plants.