• 한국미생물·생명공학회지
• /
• 제22권1호
• /
• pp.99-105
• /
• 1994

In this study we compared traditional cheesemaking process with the process utilizing ultrafiltration(UF) system. The whole milk retentates were prepared by ultrafiltration to volume concentration ratio(VCR) of 2.00:1, 2.25:1 and 2.50:1. Along with the untreated whole milk, there were studies in terms of the change of pH, titratable acidity and Soxhlet-Henkel($\circ $SH) value by mesophilic lactic starter and curd formation by rennet during Gouda cheese manufacture. Due to the increase of buffering effect titratable acidity and $\circ $SH value increased with the higher concentration ratio. When inoculated with the same volume of mesophilic lactic starter, less pH change occurred in UF retentates than in control milk. When added 0.0025% rennet, UF retentates coagulated 16~ 17 minutes ealier then the control milk. Gouda cheese yield from raw milk and UF retentates was 12.5~13.1% equally, but yield efficiency of UF retentate cheese was slightly higher than that of the raw milk cheese. Quantity of whey from retentate cheese was inversely related to VCR. But whey from retentate cheese contained higher percentage of amjor components than that from control milk cheese. In early ripening, the concentrations of lactose and soluble nitrogen compound were higher in retentate cheeses. Lactose content of control milk cheese was 3.49% and that of 2.00:1. 2.25:1, 2.50:1 VCR retentate was 3.77%, 4.89%, 7.03%, respectively. Thus, the more concentrated cheese contained a higher amount of lactose and all the lactose was hyerolyzed durion 35-day ripenion period. Soluble nitrogen compound of control milk cheese was 1.22% and that of UF cheeses was 1.82~2.06%. After 20-day ripening, soluble nitrogen compound increased starply in UF cheese.

• 대한약침학회지
• /
• 제20권1호
• /
• pp.18-22
• /
• 2017

Objectives: The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. Methods: The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at $55-60^{\circ}C$. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas aeruginosa and the Penicillium expansum species. MTT assays were used to test the cytotoxicity of the whole plant extract in the HCT-116 and the K-562 cell lines, and that extract was shown to have weak cytotoxicity in both cell lines. Conclusion: In the present study, the methanolic stem extracts of Plumbago zeylanica were found to possess remarkable antibacterial activities against many human and agricultural pathogens. The extracts were also found to possess significant antifungal activities, but the antifungal activities were less than the antibacterial activities. Finally, the extracts were found to have weak cytotoxicities in the HCT-116 and the K-562 cell lines.

• Journal of Ginseng Research
• /
• 제10권2호
• /
• pp.141-150
• /
• 1986

This study was attempted to screen the cytotoxic activity of petroleum ether ex- tract from panax ginseng root against human colon cancer cells. Two extracts of panax ginseng root, crude and partially purified, were used for this experiment. The crude extract was prepared by extraction with petroleum ether using Soxhlet aparatus for 12 to 15 hours from panax ginseng and the extract was partially purified by silicic acid column with mixture of petroleum ether: ethyl ether (70 : 30, v/v). Three species of human colon cancer cells, HRT-18, HCT-48 and HT-29, were maintained in DMEM (Dulbecco's modified Eagle medium), and the cells were cultured in DMEM containing serial concentration of the crude or partially purified fraction to observe the cytotoxic activity of the both extracts. The effects of incubation time and concentration of the both extracts in culture medium against the growth of the each cancer cell were determined. The results obtained are summarized as follows: 1. The doubling times of the HRT-18, HCT-48 and HT-29 cells were about 20, 24 and 22 hours, respectively. 2, The inhibitory effects of the crude extract on the growth of cancer cells were increased according to the rise of concentration of the extract and incubation time. 3. The inhibitory effect of partially purified fraction on the growth of HRT-18 cell was about 4 times stronger than that of the crude extract under same experimental condition. 4 The inhibitory effects of the crude and purified fraction on the growth of each cancer cell were shown difference by the kind of the cancer cell. In view of the above results, it could be said that the petroleum ether extract of panax ginseng root inhibited the division of the human colon cancer cell, in vitro.

• 한국환경생태학회지
• /
• 제29권1호
• /
• pp.1-6
• /
• 2015

In this study, the proximate composition of Pyropia dentata was compared by the analysis of minerals and amino acids that were produced at Seocheon, Chungcheongnam-do and at Wando and Jangheung, Jeollanam-do. Moisture, ash and crude proteins were analyzed using the AOAC method, and crude lipid was analyzed using the Soxhlet method, and inorganic analysis was performed using ICP-OES. Amino acid was used for the amino acid analyzer. The proximate composition analysis, moisture content, maximum values were displayed in Wando while the maximum content of ash appeared at Jangheung's Pyropia. Crude proteins was appeared at Seocheon's Pyropia and the maximum amount of crude lipid, carbohydrate was appeared in Wando's Pyropia. Inorganic analysis, calcium, potassium, magnesium, sodium, phosphorus, cobalt, chromium, copper, iron, manganese, nickel, zinc were analyzed from the Seocheon's Pyropia, however, cobalt, chromium, and nickel was not detected in Wando's Pyropia. For amino acid analysis, a total of 17 amino acids were detected: leucine, valine, aspartic acid, glutamic acid, and the amount of alanine accounted for about 57 % of total amino acids(26.1-28.7 %). The proximate composition, minerals, and amino acid of Pyropia dentata were different depending on the coastal ecological habitats.

• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 1978년도 학술대회지
• /
• pp.43-49
• /
• 1978

This study was devised to observe the cytotoxic activity of extracts of Panax ginseng root against some cancer cells and to purify the crude extract. Three kinds of cancer cells(leukemic cells L5178Y, HeLa cells and Sarcoma 180 cells) and mouse embryo cells (as normal cells) were used for this study. The ginseng roots were extracted with petroleum ether in soxhlet apparatus, and the crude extracts were purified by the silicic acid column chromatography and thin-layer chromatography methods. The results obtained are summarized as follows; 1. Eight to ten mg of the petroleum ether extract (crude extract) were obtained from 1 g of Panax ginseng root, and its activities per mg were about 1,000 units. 2. Doubling time of the L5178Y cells was increased to two fold by 24 hours incubation in culture medium containing about one ${\mu}g$ of extract per ml, and eight and ten folds higher concentration of ginseng extract were required for the Sarcoma 180 cells and HeLa cells, respectively, than for the leukemic cells(L5178Y) to inhibit the cellular growth to the same degree. 3. When the L5178Y cells were exposed to medium containing various concentration of the extract for 24 hours before initiation of the soft agar cloning procedure, about $99\%$ of the L5178Y cells were killed at concentration of 8 units per ml. 4. The growth rate of mouse embryo cell (as normal cell) was not affected by the culture with media containing various amounts (1.45 to 30.0 ${\mu}g/ml$) of the extract. 5. The crude extract could be purified about four times by silicic acid column chromatography using several solvent systems, and one spot of active compound could be obtained on the thin-layer chromatogram. 6. In the Swiss mice inoculated with Sarcoma 180 cells, a survival time of the experimental group (injection group of active compound) was extended more. 1.5 to 2.0 times than the control group's(no injection group).

• Advances in Traditional Medicine
• /
• 제9권2호
• /
• pp.142-148
• /
• 2009

Anxiety disorders are one of the serious problems which need proper therapy devoid of side effects of presently available medicines. The present study evaluates the anxiolytic and sedative activity of leaf galls of Piper nigrum Linn. in Swiss Albino mice. The pet. ether, chloroform, ethyl acetate and ethanol extracts of leaf galls of Piper nigrum Linn were obtained by continuous soxhlet extraction. The prepared extracts were found to be safe up to 2000 mg/kg body weight of mice in the acute toxicity study. Each extract was assessed for anxiolytic activity in Swiss Albino mice by elevated plus Maze, open field test, rota rod test and phenobarbitone induced sleeping time test. In the Elevated Plus Maze test, the pet.ether extract and chloroform extract at a dose of 50 mg/kg b.w. orally, significantly (P < 0.01) increased the number of entries and time spent in open arm comparable with standard diazepam at the dose of 10 mg/kg. b.w. p.o. In the open field test, pet. ether extract (50 mg/kg b.w. p.o.) showed significant increase (P < 0.01) in ambulation and activity in the center. Chloroform extract (50 mg/kg b.w p.o.) was significant (P < 0.05) for both ambulation and center activity. Pet. ether extract (50 mg/kg b.w. p.o) also showed significant activity (P < 0.01) in rota rod test. All the results are comparable with standard diazepam at the dose of 1 mg /kg b.w, p.o. Moreover all the extracts showed significant (P < 0.01) increase in the phenobarbitone induced sleeping time among which pet.ether showed more prominent activity (36%) comparable with control. The results revealed that, the active pet.ether extract and chloroform extract of leaf galls of Piper nigrum Linn is worthwhile to develop the bioactive principle for anxiolytic activity.

• 한국식품과학회지
• /
• 제35권4호
• /
• pp.653-659
• /
• 2003

황색콩과 검은콩의 원산지를 판별하기 위하여 일반성분 및 20 MHz pulsed NMR을 이용하여 자기완화시간을 측정하였다. 일반성분으로는 황색콩은 조지방 함량 만이 원산지별 차이를 나타내었고, 검은콩의 경우에는 수분과 단백질 함량이 원산지별 차이를 보였다. 20 MHz pulsed NMR을 이용하여 자기완화시간 및 고형지방함량을 측정한 결과 황색콩은 고형지방함량과 $T_2-CPMG$ 측정시 원산지에 따라 유의적인 차이를 나타내었으며, 검은콩은 $T_1-SR$을 제외한 모든 측정항목에서 원산지별 차이를 나타내었다. 황색콩과 검은콩의 원산지 판별 분석시 일반성분만으로 정준판별분석을 시행한 결과 $70{\sim}91.7%$의 정확도를 나타내었으나, NMR 자기완화시간 및 고형지방함량을 이용한 경우 100%의 정확성으로 원산지를 판별해낼 수 있었다.

• 한국약용작물학회지
• /
• 제5권2호
• /
• pp.113-118
• /
• 1997

미강에 함유된 약리성분인 ${\gamma}-oryzanol$ 함량을 검정하여 용도 다양화 연구의 기초 자료를 제공코자 시험을 수행한 바 다음과 같은 결과를 얻었다. Soxhlet 추출장치에서 얻은 미강유에서 유리 sterol과 ferulic acid ester가 분리되었다. 유리 sterol은 GC-MS에 의하여 campesterol, ${\beta}-sitosterol$ 및 stigmasterol로 구성되어 있음을 알 수 있었다. ferulic acid ester는 알칼리 가수분해에 의하여 얻어지는 검화분해물을 분리용 TLC로 분리하여 triterpene과 sterol을 얻었다. sterol은 GC-MS 측정으로 유리 sterol과 같은 조성임을 알았으며 triterpene은 cycloartanol, cycloartenol, 24-methylenecycloartanol 및 미지의 triterpene로 구성되었다. 미강유나 pitch 중 ferulic acid ester의 흡광도를 기준으로 ${\gamma}-oryzanol$의 함량을 평가한다면 큰 오차는 없으리라 생각된다.

• 한국환경과학회지
• /
• 제12권11호
• /
• pp.1167-1171
• /
• 2003

Polycyclic aromatic hydrocarbons (PAHs) are an important group of organic contaminants present in sewage sludge, due to their persistence, toxic, bioaccumulative and long range transfer. These characters make themselves as Persistent Organic Pollutants(POPs) in Long Range Transboundary Air Pollutions convention(LRTAP) of Europe. A method of the gas chromatographicmass spectrometric (GCMS) determination of PAHs present in sewage sludge was developed and applied to analyzed samples from five sewerage treatment plants (SWTPs), having different treatment types. PAHs were extracted from freeze-dried samples by toluene 16 hours in a soxhlet extraction system. The sludge extracts were cleaned-up by an activated silica gel column chromatography. The sum of the 16 US Environmental Protection Agency PAHs sewage sludge samples varied from 2.44 to 4.82 $\mu\textrm{g}$/g. Concentration of emission carcinogen PAHs(PAHcarc), such as Benzo(a)anthracene, Benzo(b)f1uoranthene, Benzo(k)f1uoranthene, Benzo(a)pyrene, Dibenzo(a,h)anthracene and Indeno( 1,2,3-cd)pyrene ranged from 0.62 to 1.03 $\mu\textrm{g}$/g. The total amount of PAHs emission from sewage sludge in Korea was calculated as a top-down approach. PAHs and $\Sigma$PAHcarc from sewage treatment plants had emitted to environment by several pathway. The highest amount of emission to the environment was remarked in the ocean dumping(PAHs and $\Sigma$PAHcarc emissions were 1155.95 kg/year and 5040.32 kg/year, respectively).

• 한국환경보건학회지
• /
• 제26권2호
• /
• pp.34-40
• /
• 2000

The purpose of this study was to investigate the seasonal variation of PAHs and to estimate their source characteristics in Taegu area. To do this, four sampling sites were selected to represent an industrial, a traffic, a traffic & residential, and a residential area in Taegu. Total of 72 samples had been collected from January, 1999 to September, 1999 on glass micro fiber filters by high volume air sampler. The PAHs in the total suspended particulate were extracted by a soxhlet process with dichloromethane and analyzed by GC/MSD, GC/FID. A statistical analysis was performed for the PAHs data set using a principal component analysis to derive important factor inherent in the interactions among the variables. The specific conclusions of this research are: 1) There was a significant seasonal and local variation in the atmospheric concentration of PAHs. The seasonal variation is winter>spring>Fall>summer, and the local variation is industrial>traffic>graffic & residential>residential area. 2) To evaluate the correlation between a measured PAHs and other affecting factors such as air pollutant concentration and meterological data, statistical analysis was performed. PAHs and other affecting factors such as air pollutant concentration and meterological data, statistical analysis was performed. PAHs have negative correlation with temperature (r=-0.593, p<0.05), radiation(r=-0.535, p<0.05), and O3(r=-0.719, p<0.05), but have positive correlation with NO(r=0.615, p<0.05) 3)Finally, multivariate analysis was performed for the PAHs dat set to identify and to estimate the source contributions of PAHs. According to results of statistical analysis, it could be identifies as three factors such as vehicular/gasoline, vehicular/diesel, and combustion in Taegu area.