• Journal of Life Science
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• v.33 no.1
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• pp.25-33
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• 2023

The fermentation process of kimchi, which is a traditional Korean food, influences the resulting compo- sition of microorganisms, such as the genera Leuconostoc, Weissella, and Lactobacillus. In addition, several factors, including the type of kimchi, fermentation conditions, materials, and ingredients, can influence the distribution of the kimchi microbial community. In this study, next-generation sequencing (NGS) of kimchi samples obtained from central (Gangwon-do and Gyeonggi-do) and southern (Jeolla-do and Gyeongsang-do) regions in Korea was performed, and the microbial communities in samples from the two regions were compared. Good's coverage prediction for all samples was higher than 99%, indicating that there was sufficient reliability for comparative analysis. However, in a α -diversity analysis, there was no significant difference in species richness and diversity between samples. The Firmicutes phylum was common in both regions. At the species level, Weissella kandleri dominated in central (46.5%) and southern (30.8%) regions. Linear discriminant analysis effect size (LEfSe) analysis was performed to identify biomarkers representing the microbial community in each region. The LEfSe results pointed to statistically significant differences between the two regions in community composition, with Leuconostocaceae (71.4%) dominating in the central region and Lactobacillaceae (61.0%) dominating in the southern region. Based on these results, it can be concluded that the microbial communities of kimchi are significantly influenced by regional properties and that it can provide more useful scientific data to study the relationship between regional characteristics of kimchi and their microbial distribution.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.42-46
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• 1998

Polymerase chain reaction (PCR) and Southern hybridization analyses were carried out to identify clones of silk worm thorn (Cudraria tricuspidata) and Rose of sharon (Hibiscus syriacus) which look like one tree with two ar three, branches or two or three different trees. For PCR five different PCR primers $(17{\sim}24\;nucleotides)$ are derived from CaMV 35S promoter, nopaline synthase terminator and coding region of thylakoid membrane protein gene. In the case of silk worm thorn, about 500 bp of PCR product was produced from DNAs of one tree or branch in the presence of 35S primer alone. Southern hybridization analysis of genomic DNAs hybridized with $^{32}P$ labeled PCR product showed that the same size of DNA fragments were hybridized with different intensities. In addition, PCR analyses using 20 different primers of OPERON 10-mer kits showed that only OPA01 primer produced PCR products of different size. These results indicate that two different trees of silk worm thorn combined to one tree. In the case of the Rose of Sharon, the same size of PCR products were produced from three different samples but Southern hybridization with the above PCR product as a probe did not show any hybridized bands. PCR analyses in the presence of OPERON 10-mers showed OPA04 and OPA13 produced different products including same sizes of products. These, results indicate that three different trees of the Rose of Sharon seem to be derived from the tree.

• Archives of Pharmacal Research
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• v.21 no.1
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• pp.35-40
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• 1998

Human cytochrome P450 4F2 shows high regioselectivity in hydroxylation of stearic acid and leukotriene $ B_4.$ As a first step of its regulation study, human cytochrome P450 4F2 genomic DNA was isolated from liver of a person who was administered clofibrate for 10 years. From Southern hybridization, restriction enzyme digestion and sequencing experiments, isolated genomic DNA fragment was found to contain around 32 Kb DNA and more than 20 Kb of $5^I$ end regulatory region. Sequences of the structural gene region revealed exon 1 and exon 2. Further regulation studies would elucidate the feedback mechanisms of the oxidative degradation of fatty acids, inflammatory response and the clearance of leukotriene B4 in the liver. Furthermore, regulation study of this gene could explain the species difference in responses to peroxisome proliferator and help in the safety evaluation of peroxisome proliferating chemicals to human being.

• Korean Institute of Interior Design Journal
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• no.32
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• pp.28-35
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• 2002

After 18th century in Korea, a tendency of the traditional folk houses shows not only the typical types but also the cultural areas in terms of each regional part. The existing folk houses are selected in order to compare the characteristic of the plans with those of the central region in Korea. Moreover this region has been a collided zone of culture and military from the old period of the Three Kingdoms, because the district of Che-chun is located between the southern part and the central one. So its culture and housing plan showed as unique forms, so-called the Culture of Frontier and Composite. Consequently the purposes of this study are to grasp the characteristic of those plans and to compare the plan types with ones of the central region. For the attainment of the above goal, this investigated study is prosecuted by means of the existing 6-folk houses in Che-chun city.

• Journal of Astronomy and Space Sciences
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• v.36 no.1
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• pp.1-9
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• 2019

In this paper we present analysis of current density when the Cluster spacecraft pass the nightside auroral region at about $4-5R_E$ from the center of Earth. The analysis is made when the inter-spacecraft separation is within 200 km, which allows all four spacecraft to be situated inside the same current sheet. On 22 February 2002, two field-aligned current (FAC) events were observed in both the southern and the northern hemispheres. The FACs were calculated with magnetic field data obtained by the four spacecraft using the Curlometer method. The scales of the FACs along the spacecraft trajectory and the magnitudes were hundreds of kilometers and tens of $nA/m^2$, respectively, and both events were mapped to the auroral region in the ionosphere. We also examined reliability of the results with some parameters, and found that our results are adequately comparable with other studies. Nevertheless, some limitations that decrease the accuracy of current estimation exist.

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.391-413
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• 1994

The northernmost type of the monogolian oak forests in the Russian Far East was studied in terma of syntaxonomy and synchorology. Hand-sorting method, computer program TWIN-SPAN, CANOCO and SYN-TAX III were engaged for the classification and data analysis. Correlation between plant communitie and environmental factors was analyzed by DCA (Detrended Correspondence Analysis) using NeCD (Net Contribution Degree) of species. Three plant communities were recognized: the Abieti holophyllae-Quercetum mongolicae, the Lespedezo-quercetum mongolicas and the Rosa ussuriensis-Quercus mongolica communit. They were included into the alliance Jeffersonio-Quercion monogolicae which is representative to the cool-Temperate forests mixed by conierous and broadleaved trees in southern Sikhote Alin. Human impact was signified as the most important factor to interpret the disjunction of plant communities of which DCA ordination yielded sharp contrasting objects (i.e. stands and species). In the amhi-Tonghae region, alaogous vegetation types such as the Jeffersonio-Quercion monogolicae of Russian Far East, the Quercion grosseserratae of Japanese Hokkaido and the Pino koraiensis-Quercion monogolicae of Koream peninusula were reviewed in terms of similarity of species composition and stratum structure. It is signified that the Jeffersonio-Quercion monogolicae was one of the continental types more similar to Korean alliance that to Japanese one.

• Journal of the korean society of oceanography
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• v.32 no.3
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• pp.128-137
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• 1997

This study presents results from a detailed sedimentological investigation of surface sediments obtained from the Korea Strait region, the southern part of the East Sea (Sea of Japan). The distribution of different types of bottom sediments is controlled by the recent fine-grained sediment transport and deposition combined with the lowerings of sea level during the last glacial period, forming a diverse mixture of organic-rich fine-grained and shelly coarse-grained sediments. In comparison to high organic concentration of fine-grained sediments in the inner continental shelf and slope areas, the shell-rich coarse-grained sediments on the outer shelf are discernible being further modified. These coarse-grained sediments are confirmed as relict resulting from the sediment dynamics during the lower sea levels of the last glacial period. Clay mineral distribution of the fine-grained sediments gives information about the transport mechanism. Presence of present-day current system (the Tsushima Warm Current) is most probable source for the fine-grained particles into the open East Sea from the East China Sea, indicating that Holocene sediment dynamics may be used to explain the observed distribution of surface coarse-grained shell-rich sediments.

• The Journal of Korean Society of Virology
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• v.30 no.2
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• pp.131-139
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• 2000

Bovine leukemia virus (BLV) is an etiological agent of chronic diseases in cows worldwide. The BLV is one of retroviruses that contain a multi-functional enzyme, reverse transcriptase produced from the pol gene in its genome. We have sequenced some regions in the pol gene of BLV proviruses found in the Southern province of Korea from samples that turned out to be BL V positives by a PCR analysis. On the 5' side of the BLV pol gene (polymerase region), it was found that there were four leucines located at every 7 amino acids. They can form a leucine zipper motif that was not same as the pol gene of Japanese BLV isolate. The sequencing result of the proviral pol gene in Korean-type BLV also revealed some mutations leading to amino acid changes such as $CCT(Pro){\to}CTC(Leu)$, $AAT(Asn){\to}AAA(Lys)$, and non-sensible variations i.e., $TCT(Ser){\to}TCC(Ser)$, $ATT(Ile){\to}ATC(I1e)$ and $ACG(Thr){\to}ACA(Thr)$. On the 3' side of the pol gene (integrase region), some nucleotide sequences were mutated and led to amino acid changes. Among them, a mutation, $GAA(Glu){\to}GAC(Asp)$ occurred in many Korean-type BLV proviruses was very interesting because the amino acid was regarded as one of the most conserved amino acids in the retroviral integrase. It was also notable that the mutation on any leucine residue did not occur, in spite of its frequent appearance.

• Journal of the Korean Society of Clothing and Textiles
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• v.36 no.7
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• pp.778-789
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• 2012

The characteristics of ancient gagye (the cubic hair style which added wigs or other materials to hair) that appeared in mural paintings were compared between Pyongyang and Jian in Goguryeo and the midlands, the northwest region, and the northeast region in China for the same period (Han to Weijin, and Northern and Southern Dynasties). Gagye in Korea and China was classified into circle type, hat type, high-bun type, and multi-bun type; in addition, Han elements, northern race elements, Goguryeo elements, and uniqueness were compared and analyzed according to regional distribution, trend periods, and style characteristics. The Han elements of ancient gagye in Korea and China appeared in the hat type, the high-bun type, and accessories that left the hair down. The northern race elements were found in the circle type and multi-bun type. The uniqueness of Goguryeo elements included a circle from the circle type, a triangle style from the hat type, an up-do style from the high burn type, and simplified hair accessories.

• Environmental Engineering Research
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• v.21 no.1
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• pp.58-68
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• 2016

The study explored droughts using the Standardized Precipitation Index (SPI) in the northwestern region of Bangladesh, which is the drought prone area. In order to assess the trend and variability of monthly rainfall, as well as 3-month scale SPI, non-parametric Mann-Kendall (MK) tests and continuous wavelet transform were used respectively. The effect of climatic parameters on the drought in this region was also evaluated using SPI, with the Southern Oscilation Index (SOI) by means of the wavelet coherence technique, a relatively new and powerful tool for describing processes. The MK test showed no statistically significant monthly rainfall trends in the selected stations, whereas the seasonal MK test showed a declining rainfall trend in Bogra, Ishurdi, Rangpur and Sayedpur stations respectively. Sen's slope of six stations also provided a decreasing rainfall trend. The trend of the SPI, as well as Sen's slope indicated an increasing dryness trend in this area. Dominant periodicity of 3-month scale SPI at 8 to 16 months, 16 to 32 months, and 32 to 64 months were observed in the study area. The outcomes from this study contribute to hydrologists to establish strategies, priorities and proper use of water resources.