• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1237-1245
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• 2004

Agaricus blazei Murill is a medicinal mushroom native to Brazil. It used to be a source of antitumor and immunoactive compounds and considered a health food in many countries. In the present study, it was examined the effects of water extract of A. blazei (WEAB) on the growth of human lung carcinoma cell line A549 in order to investigate the anti-proliferative mechanism by WEAB. Treatment of A549 cells to WEAB resulted in the growth inhibition, morphological change and induction of apoptotic cell death in a dose-dependent manner as measured by MTT assay and flow cytometric analysis. Flow cytometric analysis revealed that WEAB caused G2/M phase arrest of the cell cycle, which was associated with a down-regulation of cyclin A in both transcriptional and translational levels. WEAB treatment induced a marked up-regulation of cyclin-dependent kinase (Cdk) inhibitor p21, however, the levels of Cdk2, Cdc2, Wee1, Cdc25C and p53 expression were remained unchanged in WEAB treated cells. In addition, WEAB treatment inhibited the levels of cyclooxygenase (COX)-2 mRNA and protein without alteration of COX-l expression. Taken together, these findings suggest that WEAB may be a potential chemotherapeutic agent for the control of human lung carcinorma cells and further studies will be needed to identify the active compounds that confer the anti-cancer activity of WEAB. Once such compounds are identified, the mechanisms by which they exert their effects can begin to be characterized.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.29 no.3
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• pp.293-301
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• 2011

The ionospheric delay is the largest error source in GPS positioning after the SA effect has been turned off in May, 2000. In this study, we used 44 permanent GPS stations being operated by National Geographic Information Institute (NGII) to estimate Total Electron Content (TEC) based on pseudorange measurements phase-leveled by a linear combination with carrier phases. The Differential Code Bias (DCB) of GPS satellites and receivers was estimated and applied for an accurate estimation of the TEC. To validate our estimates of DCB, changes of TEC values after DCB application were investigated. As a result, the RMS error went down by about an order of magnitude; from 35~45 to 3~4 TECU. After the DCB correction, ionospheric TEC maps were produced at a spatial resolution of $1^{\circ}{\times}1^{\circ}$. To analyze the effect of the number of sites used for map generation on the accuracy of TEC values, we tried 10, 20, 30, and 44 stations and the RMS error was computed with the Global Ionosphere Map as the truth. While the RMS error was 5.3 TECU when 10 sites are used, the error reduced to 3.9 TECU for the case of 44 stations.

• 한국지구물리탐사학회:학술대회논문집
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• 2004.06a
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• pp.3-17
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• 2004

Space-borne Earth observation technique is one of the most cost effective and rapidly advancing Earth science research tools today and the potential field and micro-wave radar applications have been leading the discipline. The traditional optical imaging systems including the well known Landsat, NOAA - AVHRR, SPOT, and IKONOS have steadily improved spatial imaging resolution but increasing cloud covers have the major deterrent. The new Earth observation satellites ENVISAT (launched on March 1 2002, specifically for Earth environment observation), ALOS (planned for launching in 2004 - 2005 period and ALOS stands for Advanced Land Observation Satellite), and RADARSAT-II (planned for launching in 2005) all have synthetic aperture radar (SAR) onboard, which all have partial or fully polarimetric imaging capabilities. These new types of polarimetric imaging radars with repeat orbit interferometric capabilities are opening up completely new possibilities in Earth system science research, in addition to the radar altimeter and scatterometer. The main advantage of a SAR system is the all weather imaging capability without Sun light and the newly developed interferometric capabilities, utilizing the phase information in SAR data further extends the observation capabilities of directional surface covers and neotectonic surface displacements. In addition, if one can utilize the newly available multiple frequency polarimetric information, the new generation of space-borne SAR systems is the future research tool for Earth observation and global environmental change monitoring. The potential field strength decreases as a function of the inverse square of the distance between the source and the observation point and geophysicists have traditionally been reluctant to make the potential field observation from any space-borne platforms. However, there have recently been a number of potential field missions such as ASTRID-2, Orsted, CHAMP, GRACE, GOCE. Of course these satellite sensors are most effective for low spatial resolution applications. For similar objects, AMPERE and NPOESS are being planned by the United States and France. The Earth science disciplines which utilize space-borne platforms most are the astronomy and atmospheric science. However in this talk we will focus our discussion on the solid Earth and physical oceanographic applications. The geodynamic applications actively being investigated from various space-borne platforms geological mapping, earthquake and volcano .elated tectonic deformation, generation of p.ecise digital elevation model (DEM), development of multi-temporal differential cross-track SAR interferometry, sea surface wind measurement, tidal flat geomorphology, sea surface wave dynamics, internal waves and high latitude cryogenics including sea ice problems.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.66-72
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• 2003

Toxicogenomics is now emerging as one of the most important genomics application because the toxicity test based on gene expression profiles is expected more precise and efficient than current histopathological approach in pre-clinical phase. One of the challenging points in Toxicogenomics is the construction of intelligent database management system which can deal with very heterogeneous and complex data from many different experimental and information sources. Here we present a new Toxicogenomics database developed as a part of 'Toxicogenomics for Efficient Safety Test (TEST) project'. The TEST database is especially focused on the connectivity of heterogeneous data and intelligent query system which enables users to get inspiration from the complex data sets. The database deals with four kinds of information; compound information, histopathological information, gene expression information, and annotation information. Currently, TEST database has Toxicogenomics information fer 12 molecules with 4 efficacy classes; anti cancer, antibiotic, hypotension, and gastric ulcer. Users can easily access all kinds of detailed information about there compounds and simultaneously, users can also check the confidence of retrieved information by browsing the quality of experimental data and toxicity grade of gene generated from our toxicology annotation system. Intelligent query system is designed for multiple comparisons of experimental data because the comparison of experimental data according to histopathological toxicity, compounds, efficacy, and individual variation is crucial to find common genetic characteristics .Our presented system can be a good information source for the study of toxicology mechanism in the genome-wide level and also can be utilized fur the design of toxicity test chip.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.233-241
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• 2005

Plants have considerable advantages for the production of antigenic proteins because they provide an inexpensive source of protein and an easy administration of vaccine. Since a publication describing edible plant vaccine of HBsAg in 1992, a number of laboratories around the world have studied the use of plants as the bioreactor to produce antigenic proteins of human or animal pathogens. Over the last ten years, these works have been mainly focused on three major strategies for the production of antigenic proteins in plants: stable genetic transformation of either the nuclear or plastid genome, or transient expression in plants using viral vectors. As many antigenic proteins have been expressed in tobacco, also several laboratories have succeeded to express genes encoding antigenic proteins in other crop plants: potato, tomato, maize, carrot, soybean and spinach. At present many works for the production of edible plant vaccine against bacteria-mediated diseases have mostly performed the studies of enterotoxins and adhesion proteins. Also the development of new-type antigens (pili, flagella, surface protein, other enterotoxin and exotoxin etc.) is required for various targets and more efficacy to immunize against microorganism pathogens. Many works mostly studied in experimental animals had good results, and phase I clinical trial of LTB clearly indicated its immunogenic ability. On the other hand, edible plant vaccines have still problems remained to be solved. In addition to the accumulation of sufficient antigen in plants, human health, environment and agriculture regulation should be proven. Also oral tolerance, the physiological response to food antigens and commensal flora is the induction of a state of specific immunological unresponsiveness, needs to be addressed before plant-derived vaccine becomes a therapeutic option.

• Journal of the Korean Vacuum Society
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• v.21 no.3
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• pp.178-184
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• 2012

Carbon coils could be synthesized on nickel catalyst layer-deposited silicon oxide substrate using $C_2H_2$ and $H_2$ as source gases and $SF_6$ as an additive gas under thermal chemical vapor deposition system. The characteristics (formation density and morphology) of as-grown carbon coils were investigated as functions of additive gas flow rate and the cycling on/off modulation of $C_2H_2/SF_6$ flows. Even in the lowest $SF_6$ flow rate (5 sccm) in this work, the cycling on/off modulation injection of $SF_6$ flow for 2 minutes could give rise to the formation of nanosized carbon coils, whereas the continuous injection of $SF_6$ flow for 5 minutes could not give rise to the carbon coils formation. With increasing $SF_6$ flow rates from 5 to 30 sccm, the cycling on/off modulation injection of $SF_6$ flow confines the geometry for the carbon coils to the nanosized ones. Fluorine's role of $SF_6$ during the reaction was regarded as the main cause for the confinement of carbon coils geometries to the nano-sized ones.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.341-348
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• 2016

This study aimed to develop a microbial process for producing aminolevulinic acid (ALA) using crude glycerol. In the culture of ALA-producing cells (Escherichia coli/pH-hemA) in a medium containing crude glycerol, the cell density and production were 1.8-fold and 1.2-fold lower than those obtained from pure glycerol, respectively. However, the cell growth and production were improved by supplementing the medium with trehalose (30 or 100 g/l). Engineered cells (E. coli/pH-hemA/pS-otsBA) were constructed to express otsBA and their culture performance was compared with that of control cells (E. coli/pH-hemA/ pSTV28). The effects of isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration and the time of induction were examined to improve the cell growth and ALA production in engineered cells cultured using crude glycerol. When 0.6 mM of IPTG was added at the beginning of the exponential growth phase, the ALA produced by cells was 2,121 mg/l, which was comparable to that from pure glycerol. The results demonstrate that otsBA expression endowed cells with the capacity to tolerate the toxicity of crude glycerol for direct use.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.1
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• pp.35-44
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• 2005

Microclimate of Gwangneung forest watershed is characterized by analyzing wind, radiation, profiles of air temperature and humidity, soil and bole temperature, precipitation and soil water content measured at and around the flux tower from April 2000 to September 2003. Mountain-valley wind was prevalent due to the topographic effect with dominant wind from east during daytime and relatively weak wind from west during nighttime. Air temperature reaches its peak in July-August whereas monthly-averaged incoming shortwave radiation shows its peak in May due to summer monsoon. Albedo ranges from 0.12 to 0.16 during the growing season. Monthly-averaged bole temperature is in phase with monthly- averaged air temperature which is consistently higher. Monthly-averaged soil temperature lags behind air temperature and becomes higher with leaf fall. With the emergence of leafage in April, maximum temperature level during midday shifts from the ground surface to the crown level of 15-20m in May. Profiles of water vapor pressure show a similar shift in May but the ground surface remains as the major source of water. Vapor pressure deficit is highest in spring and lowest in winter. Monthly averaged surface soil temperatures range from 0 to 20℃ with a maximum in August. Monthly averaged trunk temperatures of the dominant tree species range from -5.8 to 21.6℃ with their seasonal variation and the magnitudes similar to those of air temperature. Annual precipitation amount varies significantly from year to year, of which >60% is from July and August. Vertical profiles of soil moisture show different characteristics that may suggest an important role of lateral movement of soil water associated with rainfall events.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.680-687
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• 2018

Borehole radar is a radar used for underground resources and geological exploration purposes. It needs a high-speed sampler to transmit electromagnetic waves with a pulse width of several ns and to receive reflected waves of several tens to several hundreds of MHz reflected from the object to be surveyed. ETS (Equivalent-Time Sampling), which can achieve sampling performance of several GHz with a sampling frequency of several tens of MHz, is suitable for use as a sampler of a borehole radar receiver. In this paper, we propose a method to control the sampling clock delay, which is the most important factor in ETS sampler design, using four clocks with phase difference of $90^{\circ}$ for one clock source. The proposed method can reduce the time required to acquire the data within the set interval by 1/25 than the conventional method using the delay generator. When the implemented sampler is applied to the receiver of existing borehole radar, it is possible to accumulate 58 additional times due to the shortened sampling time. In addition, by using one delay control logic compared with the conventional method using several sampling clock delay control logic in order to satisfy the target sampling range, it is possible to omit the correction process which was necessary in the past. As a result, the structure of the system can be simplified and a uniform sampler can be realized.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.5
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• pp.382-391
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• 2009

Atmospheric speciated mercury concentrations including total gaseous mercury (TGM) and reactive gaseous mercury (RGM) were measured in Chuncheon from March 2006 to November 2008. Average concentrations were 2.10 ${\pm}$ 1.50 ng/$m^3$ and 3.00 ${\pm}$ 3.14 pg/$m^3$ for TGM and RGM, respectively. RGM concentrations were higher during daytime than nighttime probably because of high photochemical activities. We found that RGM concentration considerably increased as ozone increased when fog occurred, indicating that ozone was the important oxidant for $Hg^0$ in aqueous phase. TGM concentration showed positive correlations with CO and $PM_{10}$ which can transport in long-range, but there was no correlation with $NO_2$. Considering that major source of mercury is combustion process, this result showed that local sources did not significantly impact on TGM concentration in Chuncheon. Five-day backward trajectories were calculated for the samples representing high and low concentrations of TGM, and determined that industrialized area of China including Shenyang and Beijing influenced TGM concentrations in Chuncheon.