• KSBB Journal
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• 제7권3호
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• pp.179-185
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• 1992

NAD재생형 bioreactor를 구축함에 있어 음이온 하전막을 이용하고자 하였다. 사용한 막은 용액상의 NAD의 통과를 저지하여 80.9%를 bioreactor내에 존속시켰는데 NAD의 bioreactor내에의 존속율은 Tris-maleate완충용액을 사용하고 albumin을 첨가하므로써 증가되었다. 음이온하전막을 장착한 bioreactor내에서 주반응 효소로서 sorbitol dehydrogenase와 재생반응효소로서 alcohol dehydrogenase를 사용하여 NADH로의 재생과 함께 fructose로부터 sorbitol의 생산을 행하였다. 반응형식은 반복회분조작을 행하였는데 매 회분마다 70%의 전환율이 될 때까지 반응기킨 후 반응액의 90%를 회수한 후 fructose와 ethanol을 feed하여 다음의 회분반응을 행하였다. Sobitol의 생산량은 198시간의 반응에서 47g/L이었다. 한편 사용한 막의 NAD존속율은 반응종료후에도 거의 감소되지 않았다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.220-220
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• 2005

• Archives of Pharmacal Research
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• 제21권6호
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• pp.718-722
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• 1998

Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part of Solanum lyratum Thunb. by the activity-guided fractionation employing carbon te trachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1mcM to 50mcM. Further studies revealed that at the concentration of 10mcM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.271-274
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• 1996

The antihepatotoxic activity of Bezoar Bovis and Moschus was investigated by in vitro assay method using galactosamine and carbon tetrachloride-induced cytotoxicity in primary-cultured rat hepatocytes. The antihepatotoxic activity was evaluated by measuring the level of glutamate pyruvate transaminase and sorbitol dehydrogenase which were released from the necrotic hepatocytes to the culture medium. In galactosamine-intoxicated hepatocytes, the chloroform fraction of Bezoar Bovis reduced the level of glutamate pyruvate transaminase and sorbitol dehydrogenase resulting in 65% and 59% protection, respectively. The n-Hexane fraction of Moschus resulted in 45% and 40% protection, respectively in this system. In the case of carbon tetrachloride-intoxicated rat hepatocytes, Bezoar Bovis did not have significant effect and only the aqueous fraction of Moschus showed 42% and 40% protection, respectively.

• Toxicological Research
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• 제9권2호
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• pp.253-262
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• 1993

The effects of dichloromethane (DCM) on carbon tetrachloride (CT) toxicity were examined in adult male rats. A concomitant treatment of rats with DCM (0.3, 0.6, 1.2 g/kg, po) significantly potentiated the hepatotoxicity of CT (1.0 g/kg, po) as determined by increase in serum GPT (glutamic pyruvic transaminase), GOT (glutamic oxaloacetic transaminase), and SDH (sorbitol dehydrogenase) activity 24 hr following the treatments. Serum LDH (lactate dehydrogenase) activity was increased by either DCM or CT treatment.

• 한국미생물·생명공학회지
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• 제25권1호
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• pp.68-74
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• 1997

The conversion of D-sorbitol to L-sorbose by Gluconobater suboxydans was analyzed, and continuous production of L-sorbose was carried out in immobilized cell reactors. L-Sorbose production by high densities of resting cells was more effective than by conventional batch fermentations. Sorbitol dehydrogenase, an enzyme converting D-sorbitol to L-sorbose, did not suffer from substrate inhibition, but from product inhibition. When L-sorbose production was carried out with Ca-alginate-immobilized cells, about 60 g/l of L-sorbose was obtained. On the other hand, when the corn steep liquor (CSL) concentration of medium was reduced to 0.08%, 80 g/l of L-sorbose was obtained. Outgrowth inside the immobilized carriers was thought to block the pores of the carriers so that substrate could not easily diffuse through the carriers. Continuous production of L-sorbose was well accomplished in a bubble column reactor, and 6. 5 g/l.h of productivity and 81.2% of yield were obtained at a substrate feeding rate of 0.08h$^{-1}$ under the optimum conditions with carrier volume of 55% and aeration rate of 3 vvm.

• 대한수의사회지
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• 제26권1호
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• pp.38-42
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• 1990

The effect of Aloe vera administration(500mg/kg, P.O.) for 21 days on the total white blood cell, packed cell volume, hemoglobin, total protein, serum glutamic pyruvic transaminase and sorbitol dehydrogenase values in cobalt-60 gamma irradiated rats was s

• 약학회지
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• 제42권1호
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• pp.108-113
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• 1998

G009, a polysaccharide isolated from the mycelia of Ganoderma lucidum IYO09, showed a hepatoprotective activity against $CCl_4$ induced cytotoxicity in primary cu ltured rat hepatocytes. Incubation of $CCl_4$-intoxicated hepatocytes with G009 significantly reduced the levels of glutamic pyruvic transaminase and sorbitol dehydrogenase released from hepatocytes in the medium. G009 showed antioxidative effect by elevating the activities of glutathione reductase and superoxide dismutase, and the content of glutathione in $CCl_4$-intoxidcated primary cultured rat hepatocytes. Furthermore, G009 significantly elevated glutathione-S-transferase activity in $CCl_4$-intoxicated primary cultured rat hepatocytes. G009 also reduced the production of malondialdehyde, a byproduct of lipid peroxidation. From these results, it could be concluded that G009 exerted hepatoprotective activity against $CCl_4$-induced cytotoxicity through antioxidation.

• Journal of Pharmaceutical Investigation
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• 제9권4호
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• pp.23-28
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• 1979

The effect of maltitol admininstration to rat was studied on breakdown of ${\alpha}-glycoside$ linkage with intestinal mucosa or pancreatic enzymes and induction of hepatic polyol dehydrogenase activities. Maltitol was contained as 13% or 26% in diet, and was administrated to rat for 9 weeks. This report carried out that ${\alpha}-glycoside$ linkage of maltitol was not hydrolyzed with pancreatic enzymes and intestinal mucosa. Maltitol dehydrogenase was not obeserved in liver cytoplasm, and hepbtic sorbitol dehydrogenase was not induced by maltitol administsation. Also, the effect of maltitol on aging of aluminum hydroxide gel, prepared by the reaction of aluminum chloride solution with strong ammonia solution to final pH 7.0, was studied by potentiometric titration, pH and acid-consuming capacity. Gel containing 1% or 2% maltitol was lost less than 2% of their acid-consuming capacity during a 120 days aging period compared with a loss of more than for an identical gel without maltitol and gel containing 0.5% maltitol.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.86-86
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• 2002

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