• Natural Product Sciences
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• v.13 no.3
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• pp.255-257
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• 2007

A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds kurarinone and trifolirhizin from the roots of Sophora flavescens using a binary gradient of acetonitrile : $H_{2}O$ with UV detection at 254 nm. The kurarinone and trifolirhizin contents of the roots of S. flavescens collected from ten district markets in Korea and China were $77.24{\sim}686.89$ ${\mu}g/g$ and $46.89{\sim}288.58$ ${\mu}/g$, respectively.

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.491-495
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• 1997

All fifteen flavonoids (1-15) have been isolated from the roots of Sophora flavescens (Leguminosae) as active principles of the cytotoxic property toward human tumor cell lines such as A549, SK-OV-3, SK-Mel-2, XF498 and HCT15, in vitro. By means of spectral analyses, particularlyby the aid of various two dimensional NMR experiments, all $^1H-NMR$ ad $^{13}C$ -NMR signals of 1-15 were completely assigned, and thus the structures of 1-15 were established unambiguously.

• Fashion & Textile Research Journal
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• v.2 no.2
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• pp.113-117
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• 2000

The extracts drawn out from Sophora japonica by methanol was concentrated. Using this concentrate the silk was dyed, 400% conc. of dyeing material, $80^{\circ}C$ temperature of dyeing, 30 min time of dyeing. The K/S value of silk fabric was the most efficient for the simmordanting method. On the other hand, the amount of absorbed mordant the Cu-mordant was largest mordants. The surface colors on the dyed fabric depended heavily upon mordants or mordanting methods. For all cases, the value of the dyed fabric was dark. for the mordanting, color difference was distinct when using the Fe-mordant. Lightfastness of the color fastness was best in Cu-mordant. The Fe-, Cu- mordant showed the greatest antimicrobial activity.

• Biomolecules & Therapeutics
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• v.11 no.1
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• pp.5-7
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• 2003

The purpose of this study was to evaluate tyrosinase inhibitory activity of plant for extracts for cosmetic use. When 80 plant extracts were tested, the methanol extracts of Allium thunbergi, Asparagus oligoclonos, Ixeris dentate, Salvia plebeia, Sophora flavescens and Sophora japonica showed more than 30% inhibition of mushroom tyrosinase activity at 100 $\mu\textrm{g}$/mL. Although less active than the reference compound, kojic acid ($IC_{50}$=7.0-16.3 $\mu\textrm{g}$/mL), these plant extracts may be used as tyrosinase inhibitors in cosmetics.

• Advances in Traditional Medicine
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• v.4 no.3
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• pp.211-214
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• 2004

The inhibitory effect of dl-puerol A as but-2-enolide isolated from Sophora japonica was investigated on copper ion-induced protein oxidative modification in vitro. It inhibited copper-induced protein oxidative modification. However, its inhibitory effect was a little weaker than that of $dl-{\alpha}-tocopherol$ as an antioxidant. The results demonstrated that dl-puerol A, one of but-2-enolides, might be of use in the oxidative stress.

• Natural Product Sciences
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• v.7 no.1
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• pp.5-8
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• 2001

Lupenone, hexadecyl ferulate, (-)-sophocarpine and three isoflavonoids such as genistein, 3'-methoxydaidzein and calycosin were isolated from the roots of Sophora flavescens.

• Biomedical Science Letters
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• v.13 no.3
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• pp.189-195
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• 2007

This study was carried out to examine antimicrobial substances from medicinal plants, the ethanol extracts of 38 medicinal plants were tested for the antimicrobial activity against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus ATCC 43300. The extracts of Glycyrrhiza uralensis, Sophora flavescens, Dryopteris crassirhizoma, and Pinas densiflora showed significant antimicrobial activities against both S. aureus ATCC 25923 and methicillin-resistant S. aureus ATCC 43300. The extract of Dryopteris crassirhizoma among these medical plants showed the highest antimicrobial activity. These results suggested that the extracts from Dryopteris crassirhizoma, Sophora flavescens, Pinas densiflora, and Glycyrrhiza uralensis could be the potential source of antimicrobial agents against methicillin-resistant S. aureus and S. aureus.

• Archives of Pharmacal Research
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• v.26 no.12
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• pp.1009-1013
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• 2003

A new pterocarpan, (-)-maackiain 3-sulfate (1) was isolated from the methanol extract of roots of Sophora subprostarata together with (-)-maackiain(2), trifolirhizin (3), lupeol (4), ononin (5),7,4'-dihydroxyflavone (5), and (+)-syringaresinol (7). The structure of 1 was determined by analyses of 2D NMR and HRFABMS. Compounds 5-7 were isolated from this plant for the first time.

• Archives of Pharmacal Research
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• v.18 no.1
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• pp.41-43
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• 1995

The linkage pattem of two side chains i.e., a isopentenyl and a lavandulyl group in kushenol M(I), a flavonoid from Sophora flavescens was established by the aid of 2-D NMR techniques, especially DEPT, ${13}C-^1H$ COSY and COLOC experiments. Thus, I was unequivocally determined as (2R, 3R)-5, 7, 2', 4'-tetrahydroxy-6-isopentenyl-8-lavandulyflavanonol.

• Journal of Haehwa Medicine
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• v.5 no.2
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• pp.503-507
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• 1997

The cytotoxicity-guided fractionation of the roots of Sophora flavescens (Leguminosae) extracts led to the isolation of fifteen active principles 1~15, responsible for the cytotoxicity against five kinds of cultured human tumor cell lines, i.e., A549(non small cell lung), SK-OV-3(ovary), SK-MEL-2(skin), XF498(central nerve system) and HCT-15(colon), evaluated by SRB method in vitro. Compounds 2~14 were classified as unusual flavonoid occurred exclusively in this species and the proliferation of each examined tumor cells were significantly inhibited during the continuous exposure to compounds 1~15 for 48 hours, respectively.