• Title/Summary/Keyword: solid media

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A Study of Organic Acid Formation and Dialysis Culture in E. coli Fermentation (대장균 배양에서의 유기산 생성과 투석배양에 관한 연구)

  • 김인호
    • KSBB Journal
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    • v.8 no.4
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    • pp.382-389
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    • 1993
  • In order to elucidate the effect of acetate ion on the growth of Escherichia coli, flask and fermentor cultures were performed using M9 and LB media. Acetic acid was secreted at a higher rate under the conditions of high glucose concentration as well as of richer medium, i. e., LB broth. The pH in flask culture could not be controlled as i fermentor and pH decreased with the formation of acetic acid. The inhibition effect of acetic acid was pronounced at a lower pH, and the effective inhibitory concentrations of acetic acid were 2.0g/l for LB flask culture, 4.0g/l for M9 flask culture, and 8.0g/l for M9 fermentor culture. Dialysis flask culture was designed to slowly provide E coli cells with glucose. Solid LB agar was layered under LB liquid medium with the variation of agar concentration and solid volume, The increase in the solid portion in the total volume(agar+liquid) resulted in the increase of the final cell concentration. This can be ascribed to the fact that the larger solid phase behaves like a larger reservoir for glucose and controls the growth of E. coli with a controlled rate.

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Growth Performance of Sedum reflexum and Physical Properties of Extensive Green Roof Growing Media (경량형 옥상녹화 식재기반의 물리성과 레플렉숨 생육특성)

  • Li, Hong;Kang, Tai-Ho
    • Journal of the Korean Institute of Landscape Architecture
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    • v.42 no.6
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    • pp.50-59
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    • 2014
  • This experiment was performed in order to study the relationship between physical properties of soil and the growth characteristics of Sedum reflexum. A correlation analysis and multiple regression analysis were performed using SPSS Ver 19.0 for Windows. The multiple regression analysis results of soil physical properties and growth characteristics were as follows. The regression equation: The length=$.993-14.070^*$(soil bulk density)+$.233^*$(solid phase)+$.038^*$(liquid phase)+$.068^*$(permeability). The significance of soil bulk density and solid phase was great. The width=$2.931-33.925^*$(soil bulk density)+$.566^*$(solid phase)+$.206^*$(liquid phase)+$.027^*$(permeability). The significance of soil bulk density and solid phase was great. The wet weight and dry weight of the upper and lower and soil physical properties did not have a direct relationship.

Characteristics of mycelial growth and fruit body of Sparassis latifolia strains and selection of suitable incubation conditions in liquid spawn

  • Lee, Yunhae;Gwon, Heemin;Jeon, Daehoon;Choi, Jongin;Lee, Youngsoon
    • 한국균학회소식:학술대회논문집
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    • 2018.05a
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    • pp.29-29
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    • 2018
  • Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

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A Study on the Types of Virtual Influencers in China Using Q Methodology

  • LILI;Jong-Yoon Lee;ShanShan LIU;Jang Sun Hong
    • International Journal of Advanced Culture Technology
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    • v.11 no.2
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    • pp.152-161
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    • 2023
  • Modern people live a life connected with the real world and the virtual world by relying on the new media of enterprises and social consumption led by innovative technologies. In this environment, virtual influencers actively communicate with consumers and build relationships through social media, which is a new marketing tool that has attracted widespread attention. From a business perspective, it is necessary to have a solid understanding of this phenomenon, and then explore communication strategies to effectively develop virtual influencers. To investigate followers' preference for virtual influencers, this study employs the Q-method, which studies human subjective attributes, an empirical research effort to uncover complex issues in human subjectivity. To determine the factors that trigger people's voluntary and active practice and the preference degree of virtual influencers, the Q method is implemented to examine human subjectivity, thoughts and attitudes. According to the results of this study, virtual influencers are a new group of idols full of vitality. The interviews found that there are still many virtual influencers who do not know about followers, but each type can be clearly understood through the intuitive understanding of the interviewees. Divided out, type 1 one egoideal virtual influencers aim to represent an idealized version of the creator or target audience. Embodies ideal physical characteristics, personality or lifestyle desired by the audience. Type 2 is charismatic and attractive, and has the characteristics of most virtual influencers. It is suggested that it can be developed into a potential type, doing brand cooperation, and content production on social media platforms. Type 3: Game animation, derived from the image of characters in games or comics, with stylized features and energetic personalities, which can be integrated into games or entertainment experiences. Type 4 development potential type is the most successful type among virtual imagers, and it is also the purpose of marketing virtual influencers. It is essential that brand endorsement on social media platforms, integrated marketing, and driving advertising traffic. It is recommended to improve production technology to reduce investment costs.

The Cultural Characteristics of Paecilomyces tenuipes (눈꽃동충하초의 배양적 특성)

  • Kang, Bok-Hee;Lee, Sang-Han;Hur, Sang-Sun;Shin, Yong-Kyu;Lee, Dong-Sun;Chang, Hung-Bae;Song, Bong-Jun;Lee, Jin-Man
    • Food Science and Preservation
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    • v.17 no.3
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    • pp.365-369
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    • 2010
  • This study was conducted to investigate the cultural characteristics of Paecilomyces tenuipes PJ-1 separated from the nature. We investigated the effect of media, pH and temperature in growth of Paecilomyces tenuipes PJ-1 on solid culture media and the effect of inoculum size, glass beads in liquid culture condition. Paecilomyces tenuipes showed the most favorable growth on PDA among 5 different media. The optimum growth pH and temperature were at pH 6.0 and $25^{\circ}C$ on solid culture. Mycelial growth of P. tenuipes decreased rapidly above $35^{\circ}C$ and under $15^{\circ}C$. In liquid culture, the optimum inoculum size was 10.0% and the SED and PMV value were increased with adding glass bead and glass bead size in the range of glass bead 0~50 ea and size 3, 5 mm.

Analysis of the Effect of Media Types and Chromagenic Chemicals on the Detection of Extracellular Laccase Activity among Lentinula edodes Strains (표고 교잡균주들의 세포외 laccase 활성 검출에 미치는 배지성상과 발색반응 시약의 영향 분석)

  • Kim, Jun-Young;Kwon, Hyuk-Woo;Tang, Longqing;Ko, Han-Kyu;Kim, Seong-Hwan
    • The Korean Journal of Mycology
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    • v.39 no.1
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    • pp.48-52
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    • 2011
  • Breeding of Lentinula edodes generates a number of hybrid strains that are subject to evaluation for good traits for the mushroom production. As an effort to understand biochemical properties of the hybrid strains, this study tried to develop a fast and easy method for comparison of the ability of producing extracellular laccase among hybrid strains of Lentinula edodes. For this aim, we estimated the effect of media types and chromagenic chemicals on the detection of extracellular laccase in seven hybrid strains of L. edodes. When Remazol Brilliant Blue R (RBBR) dye was used for chromagenic reaction, the detection of the enzyme activity was feasible both in the solid and liquid media containing not potato dextrose but malt extract as a nutrient component. When guaiacol was used for chromagenic reaction, the detection of the enzyme activity was feasible both in the solid and liquid media containing either potato dextrose or malt extract as a nutrient component. Malt extract-based liquid culture with RBBR or guaiacol in 2 ml microfuge tube allowed us to economically and quantitatively detect and compare the enzyme activity within 3 days among the tested hybrid strains of L. edodes.

Feasibility Study on Removal of Total Suspended Solid in Wastewater with Compressed Media Filter (압축성 여재 여과를 이용한 하수의 고형물질 제거 타당성 연구)

  • Kim, Yeseul;Jung, Chanil;Oh, Jeill;Yoon, Yeomin
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.2
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    • pp.84-95
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    • 2014
  • Recently, as a variety of techniques of CMF (Compressed media filter) that has advantages of high porosity and compressibility have been developed in the U.S. and Japan. Therefore, the interest of intensive wastewater treatment using CMF has grown. This study examined the feasibility of CMF with varying sewage water quality to determine the optimum operating conditions. A preliminary tracer test that investigated the filtering process under various compression and flow rate conditions was performed. In a high compression condition, different porosities were applied to each depth of the column. Therefore, a distinct difference between a theoretical value and results of tracer test was observed. For the TSS (Total suspended solid) removal and particle size distribution of CMF for pre-treatment water under the various compression conditions, the compression ratio of 30 percent as the optimal condition showed greater than 70% removal efficiency. In addition, the compression ratio of >15% was required to remove small-sized particles. Also, an additional process such as coagulation is necessary to increase the removal efficiency for < $10{\mu}m$ particles, since these small particles significantly influence the effluent concentration. Modeling results showed that as the compression rate was increased, TSS removal efficiency in accordance with each particle size in the initial filtration was noticeably observed. The modeling results according to the depth of column targeting $10{\mu}m$ particles having the largest percentage in particle size distribution showed that 150-300 mm in filter media layer was the most active with respect to the filtering.

Effects of Liquid Surface Tension on the Heat Transfer Coefficient in a Three-Phase Slurry Bubble Column (삼상슬러리 기포탑에서 액상의 표면장력이 열전달 계수에 미치는 영향)

  • Lim, Ho;Lim, Dae Ho;Jin, Hae-Ryong;Kang, Yong;Jung, Heon
    • Korean Chemical Engineering Research
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    • v.50 no.3
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    • pp.499-504
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    • 2012
  • Characteristics of overall heat transfer were investigated in a three-phase slurry bubble column with relatively low surface tension media, which has been frequently encountered in the fields of industry. The heat transfer phenomena was examined in the system which was composed of a coaxial vertical heater and a proper of bubble column. The heat transfer coefficient was estimated from the measured mean value of temperature difference between the heater surface and the column proper at the steady state condition. Effects of gas velocity ($U_G$), solid fraction in the slurry phase ($C_S$) and surface tension (${\sigma}_L$) of continuous liquid media on the overall heat transfer coefficient (h) in the bubble column were determined. The mean value of temperature difference was estimated from the data of temperature difference fluctuations with a variation of time. The amplitude and mean value of temperature difference fluctuations with respect to the elasped time appeared to decrease with decreasing the surface tension of liquid phase. The overall heat transfer coefficient between the immersed heated and the bubble column increased with an increase in the gas velocity or solid fraction in the slurry phase, but it decreased with an increase in the surface tension of continuous liquid media. The overall heat coefficient in the slurry bubble column with relatively low surface tension media was well correlated in term of operating variables and dimensionless groups within this experimental conditions.

An performance analysis on SSD caching mechanism in Linux (리눅스 SSD caching mechanism 의 성능 비교 및 분석)

  • Heo, Sang-Bok;Park, Jinhee;Jo, Heeseung
    • Smart Media Journal
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    • v.4 no.2
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    • pp.62-67
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    • 2015
  • During several decades, hard disk drive(HDD) has been used in most computer systems as secondary storage and, however, the performance enhancement of HDD is limited by its mechanical properties. On the other hand, although the flash memory based solid state drive (SSD) has more advantages over HDD such as high performance and low noise, SSD is still too expensive for common usage and expected to take several years to replace HDD completely. Therefore, SSD caching mechanism using the SSD as a cache of high capacity HDD has been highlighted lately. The representatives of SSD caching mechanisms are typically bcache, dm-cache, Flashcache, and EnhanceIO. Each of them has its own internal mechanism and implementation, and this makes them to show their own pros. and cons. In this paper, we analyze the characteristics of each SSD caching mechanisms and compare the performance of them under various workloads. We expect that our contribution will be useful to enhance the performance of SSD caching mechanisms.

Measurement of the Complex Permittivies of Various Dielectrics Using an Open-Ended Coaxial Probe (개방단말 동축선 프로브를 이용한 다양한 유전체의 복소 유전율 측정)

  • Shin, Hyun;Hyun, Seung-Yeup;Kim, Sang-Wook;Kim, Se-Yun
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.38 no.7
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    • pp.22-27
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    • 2001
  • In this paper, the complex permittivity of various dielectrics such as powder(sugar and flour) and solid(teflon and acrvl) are measured by using an open ended coaxial-line probe, which is self designed and manufactured, The probe is connected to a vector network analyzer(VNA) through a coaxial cable, The end of the cable is corrected by using an OSL(open, short, and load) calibration kit, The phase difference, which is produced by inserting the probe at the end of the line. is compensated by using the numerically calculated reflection coefficient of distilled water, The complex permittivity is reconstructed by inserting the measured reflection coefficient, which is produced at the interface between the probe and measuring material, into ,an virtual conical cable conversion model. Over a wide frequency range from 30 MHz to 3 GHz, the measured complex permittivitis of various powder and solid using the our method are compared with the results, which are measured by using an transmission-line method of the Korea Research Institute of Standards and Science(KRISS).

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