• Weed & Turfgrass Science
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• v.7 no.3
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• pp.259-268
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• 2018

This study was conducted to evaluate the effect of liquid fertilizer containing humic acid and Saccharomyces cerevisiae broth (LHS) on changes of turfgrass growth by investigating visual quality, chlorophyll content, dry weight of clipping, and nutrient content in leaf tissues. Treatments were designed as follows; control fertilizer (CF), HS-1 ($CF+1.0mL\;m^{-2}\;LHS$), HS-2 ($CF+2.0mL\;m^{-2}\;LHS$), and HS-3 ($CF+4.0mL\;m^{-2}\;LHS$). After treatment of LHS on creeping bentgrass, soil pH in the treated plots was decreased than that of CF. As compared to CF, visual quality, chlorophyll content and content of N, P and K were not significantly different in the LHS treatments. However, clipping yield and phosphorus uptake of HS-2 were significantly increased by 22% and 33%, respectively. These results showed that application of LHS improved the phosphorus uptake and growth of creeping bentgrass, which would be an alternative management tool for the cool season turfgrass under stress conditions.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.107-113
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• 2013

The black soldier fly larvae are able to decompose various organic wastes such as livestock manures and food wastes. We tested whether the quality of the insect derived compost, i.e. larval feces, was comparable to that of a commercial fertilizer. Chemical analysis of Hermetia. illucens compost was suitable as a fertilizer. When the kidney bean (Phaseolus vulgaris L.) was raised on the culture soil treated with the H. illucens compost, the growth of leaf area, total dry weight and leaf dry weight increased significantly. The H. illucens compost is thought to act as an increasing factor of RGR (relative growth rate) from the beginning of growth and also had a great effect on the relative growth rate throughout the late stage of growth. There is high statistical significance between NAR (net assimilation rate) and RGR (relative growth rate), but no significance between RGR and LAR (leaf assimilation rate) of the treatments. In addition, the treatment of H. illucens compost promoted the thickness of leaves from the beginning of growth and the tendency of thickening leaves from the beginning of growth and the effect continued throughout the late period of growth. When mixed with 50% of H. illucens compost rather than 25%, it showed the greater effect on the plant growth.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.37-37
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• 2018

We use two different methods for laboratory propagation from seed of lady's slipper orchid(Cypripedium macranthos Sw.); immature seed which also called green capsule or fully mature seed about 120~130 days from pollination. In green capsule culture, the seed pods should be collected within precisely right time. The right time of seed collection could be diverse under the wether conditions or nutritional factors of the plants. In fully matured seed culture, the more complicated procedures are needed to break the dormancy of the seed; thermal or chemical treatment. The seedlings in this study were easily germinated from immature seeds in Harvais medium; 53 days after pollination(DAP) in Cypripedium pubescens, DAP 65 in C. parviflorum and C. macranthos. The germinated seedlings were transplanted to hormone free media immediately to avoid abnormal growth of seedlings. When the seedlings have roots with a minimum length of around 2-3cm and have visible dormant buds, the seedlings were removed from the flask and stored in refrigerator for vernalization. To examine the correlation of seedlings and maternal plants, the 125 seedlings of C. macranthos were transplanted in the soil bed at a distance of 20-100 cm from mother plants on April 20. The survival rate of seedlings were 92% in 20 cm distance from the ripe plants, and 56 % in 100 cm distance. The seedlings which were transplanted near mother plants showed vigorous growth in plant height, leaf width, and especially dormant buds. Considering the existence of mycorrhiza which is a symbiotic association between a fungus and the roots of a orchid vascular, the various fungus from mother plants could affect the growth of the seedlings. These results indicate the possibility of high and stable production and practical industrialization of endangered lady's slipper orchids.

• Korean Journal of Medicinal Crop Science
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• v.20 no.5
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• pp.359-364
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• 2012

This study were carried out to find bolting response of cultivation in different regions and to isolate FLC (FLOWERING LOCUS C) homologs in Angelica gigas Nakai. The mean temperature of different regions, ordering in altitude, were as follows: 100 m > 350 m > 530 m > 700 m. The largest amount of rainfall was occurred in the region of 350 m while the longest time of sunshine was occurred in the region of 100 m. The content of soil chemical properties in regions showed pH 6.2 ~ 7.4, T-N 0.17 ~ 26, organic mater $1{\sim}32gkg^{-1}$, $P_2O_5$ ${151{\sim}664_{mgkg}}^{-1}$, exchangeable potassium and calcium and magnesium were 0.78 ~ 1.15, 3.9 ~ 10.0, ${0.7{\sim}3.2_{cmol}}^{+kg-1}$. L5 line of A. gigas was occurred in bolting at all regions, but the bolting ratio was 60.0% in 700 m region with non-mulching treatment. Manchu of A. gigas was not occurred in bolting at all regions. The accumulation bolting ratio of L5 line by non-mulching was higher than that of mulching as 90.4% and 72.8% in 100 m region. The MADS-box transcription factor FLC is one of the well-known examples as a strong floral repressor. We decided to isolate FLC homologs from A. gigas as a starting point of flowering mechanism research of this plant. We have isolated two RT-PCR products which showed very high amino acid sequence homology to Arabidopsis FLC.

• Korean Journal of Microbiology
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• v.32 no.1
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• pp.91-95
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• 1994

EagBI is a type II restriction endonuclease from Enterobacter agglomerans strain CBNU45 isolated from soil. EagBI was partially purified by DEAE-cellulose, phosphocellulose P11 and hydroxylapatite column chromatography. EagBI recognizes and cleaves the sequence 5'-CGAT${\downarrow}$CG-3' and generates 2-base 3'-protruding cohesive ends. The optimal reaction conditions of EagBI are 10 mM Tris-HCl (pH 7.8), 6-10 mM $MgCl_2$, at 37 ${\circ}C$. The enzyme is maximally active in the absence of NaCl, able to cleave both $dam^-$ and $dam^+$ DNAs, and sensitive to heat treatment (at 65 ${\circ}C$ for 10 min). Therefore, although EagBI is an isoschizomer of PvuI, it is more useful than PvuI in respect of the NaCl requirement and heat-stability.

• KSBB Journal
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• v.14 no.6
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• pp.718-723
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• 1999

In order to treat of alkaline dye-processing wastewater, alkalophilic strains biodegrading azo dye, Acid red 1, is isolated from natural system, and optimal culture conditions are examined using response surface analysis, statistical analysis system program. 15 different species which grow in alkaline culture media are isolated from the effluent and river soil discharged from wastewater treatment plant in dye industrial complex. One strain which has the best decolorization efficiency is chosen, and named as AR-1. The result of the examination of carbon, nitrogen and phosphorus sources which have influence on growth and decolorization reveals that optimum carbon, nitrogen and phosphorus sources are 1.0% fructose, 1.0% polypeptone, 1.0% yeast extract and 0.5% $K_2HPO_4$, respectively. In order to optimize of biodegradation conditions of dye by response surface analysis, the characteristics of decolorization and cell growth according to culture temperature and time are monitered. The result shows that the one is optimum 34.77$^{\circ}C$ for 12.97 hours; the other at 34.73$^{\circ}C$ for 12.96 hours. While, optimal conditions of culture that satisfy both cell growth and decolorization are the temperatures from 32.86$^{\circ}C$ to 36.36$^{\circ}C$ and the period of 10.96 to 15.75 hours, respectively.

• Journal of Environmental Science International
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• v.17 no.3
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• pp.287-296
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• 2008

A comprehensive quality survey for heavy metals, organophosphorous and organochlorine residual pesticides, and coplanar PCBs in samples such as water, sediments and soils as well as spiders has been implemented. The samples were undertaken at nine stream sites and their vicinity in August 2006, representing different surrounding environments. The levels of PCBs were expressed as concentrations and WHO-TEFs. Among 12 coplanar PCBs as target compounds in this study, 2,3', 4,4', 5-PentaCB (IUPAC # 118) was the congener with the highest concentration. The total concentrations and TEF values of coplanar PCBs in Siheung stream sediment (heavy industrial complex site located in Ansan city) were 3915.50 pg/g and 0.8366 pg-TEQ/g on a dry weight basis, respectively. Such levels were around 40 times higher compared to sediment from Gapyung stream (green site located in the upper of Myunggi mountain). It is probably due to the direct input of PCBs trom PCBs treatment materials. Organophosphorous (EPN, dementon-s-methyl, diazinon, parathion, and phenthoate) and organochlorine (alpha-BHC, aldrin, 4,4'-DDT, 4,4'-DDE, endosulfan alpha and etc.) pesticides were not detected above 5 ppb of detection limits. The concentrations of Cu and Cd in water and sediment samples from Siheung stream were 44.11 and $0.17ug/m\ell$ and $713.42{\mu}g/g$ and $3.73{\mu}g/g$, respectively, which contained $20\sim40$ times higher concentrations than those from Gapyung stream. In addition to the water and sediment samples, the levels of heavy metals in spider from designated sampling sites were also determined. Heavy metals in spider collected near Siheung stream was appeared to be equivalent and/or a little higher levels with respect to other spiders. Furthermore, the ratio of relative heavy metals (Cu, Cd, and Pb) in spider from each stream site showed a correlation as similar as that of heavy metals in soil samples.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.369-376
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• 2006

This research was conducted to evaluate the effect of root medium formulations on growth and nutrient uptake of hot pepper 'Knockgwang' in 72-cell plug trays. To achieve this, the nine root media were formulated by adjusting blending rate of perlite (PL) to coir (CO), peatmoss (PM) or coir+peatmoss (5:5, v/v, COPM). Then, the growth characteristics and tissue nutrient contents were determined at 35 and 70 days after sowing. The elevated blending rate of PL to CO increased fresh and dry weight of hot pepper at 35 days after sowing. The treatments of 20% in blending rate of PL to PM or that of 0% to COPM produced the highest fresh and dry weight among perlite treatments of PM or COPM. The results of crop growth at 70 days after sowing also showed similar trends to those of 35 days after sowing. The elevated blending rate of PL to CO or PM decreased tissue $P_2O_5$ and K contents and increased Ca and Mg contents at 35 days after sowing, With the equal blending rate of perlite, the plant tissue grown in CO had higher K contents and lower N, Ca and Mg contents than those in PM and COPM. The elevated blending rate of perlite in three organic matter also decreased tissue $P_2O_5$ and K contents at 70 days after sowing, but Ca and Mg contents were the highest in the treatment of 20% PL in CO, 40% PL in PM and 40% PL in COPM among perlite treatments in each organic matter.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.2 no.1
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• pp.53-62
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• 1982

An effort of preliminary type has been made to develope a practical method for the waterway area determination of a drainage outlet in rural or agricultural areas. The Seoul meteorological station was selected as tile index station, and the maximum rainfalls-duration-frequency (R-D-F) relation of short-time intense rainfalls was first established. A frequency analysis of the daily rainfalls for the 75 stations selected throughout the country resulted the 50-year daily rainfall for each station. The rainfall factor, which is defined here as the ration of 50-year daily rainfalls of individual station and the index station, was determined for the 8 climatological regions divided in this study. Following the US SCS method the runoff number of a watershed was given based on the soil type, land-use pattern, and the surface treatment. With this runoff number and the R-D-F relationship the runoff factors for the index station were computed and hence a nomogram could be drawn which makes it possible to determine the runoff factor for a given rainfall number and a rainfall of specific duration and frequency. With this done, the potential runoff of a watershed for a given rainfall duration could be calculated, based on the unit hydrograph theory, by multiplying the rainfall factor, the runoff factor, and the drainage area of the watershed under consideration. Then, the maximum runoff potential was determined by varying the rainfall duration and finding out the duration which results the peak discharge of a gived return period.

• Journal of Bio-Environment Control
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• v.3 no.1
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• pp.58-65
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• 1994

It is necessary to effective temperature and humidity management for normal growth of crops in protected cultivation during the summer season. Because the highest temperature of vinyl house inhibit normal growth of crop and decrease of crop production or marketability in summer season. Finally, the vinyl house was impossible some crop cultivation in summer season. This study was conducted to investigate effective and economic method for temperature drop in protected cultivation during the summer season. 1. In medium size vinyl house(5$\times$13$\times$3m), the effect of temperature drop appeared the highest in treatment of shading with aluminium thermal curtain＋fog system＋ventilation with fan. The effect of temperature drop was about 1$0^{\circ}C$ lower than outer air temperature and about 4$^{\circ}C$ lower than outer soil temperature. 2. The effect of temperature drop according to shading with aluminium thermal curtain＋fog system＋ventilation with fan during the highest temperature of summer season Jul., 20 to Aug., 21 was appeared about 8$^{\circ}C$ lower than outdoor above ground(1.2m) and about 7$^{\circ}C$ lower than outdoor surface ground. 3. The changes of solar radiation during a day according to shading with aluminium thermal curtain＋ventilation with fan and shading with black curtain＋ventilation with fan treatments was appeared respectively about 29.3%, 32.5% of outdoor solar radiation a fine day and respectively about 27.4%, 31.8% of outdoor solar radiation a cloudy day.