• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.753-759
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• 2023

The genus Paenibacillus contains a variety of biologically active compounds that have potential applications in a range of fields, including medicine, agriculture, and livestock, playing an important role in the health and economy of society. Our study focused on the bacterium SS4^T (KCTC 43402^T = GDMCC 1.3498^T), which was characterized using a polyphasic taxonomic approach. This strain was analyzed using antiSMASH, BAGEL4, and PRISM to predict the secondary metabolites. Lassopeptide clusters were found using all three analysis methods, with the possibility of secretion. Additionally, PRISM found three biosynthetic gene clusters (BGC) and predicted the structure of the product. Genome analysis indicated that glucoamylase is present in SS4^T. 16S rRNA sequence analysis showed that strain SS4^T most closely resembled Paenibacillus marchantiophytorum DSM 29850^T (98.22%), Paenibacillus nebraskensis JJ-59^T (98.19%), and Paenibacillus aceris KCTC 13870^T (98.08%). Analysis of the 16S rRNA gene sequences and Type Strain Genome Server (TYGS) analysis revealed that SS4^T belongs to the genus Paenibacillus based on the results of the phylogenetic analysis. As a result of the matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) results, SS4^T was determined to belong to the genus Paenibacillus. Comparing P. marchantiophytorum DSM 29850^T with average nucleotide identity (ANI 78.97%) and digital DNA-DNA hybridization (dDDH 23%) revealed values that were all less than the threshold for bacterial species differentiation. The results of this study suggest that strain SS4^T can be classified as a Paenibacillus andongensis species and is a novel member of the genus Paenibacillus.

• Geomechanics and Engineering
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• v.34 no.2
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• pp.115-124
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• 2023

By conducting three-dimensional simulation with consideration of small-strain characteristics of soil stiffness, the effects of excavation geometry and tunnel cover to diameter ratio on deformation mechanisms of an existing tunnel located either at a side of basement or directly underneath the basement were systematically studied. Field measurements were used to verify the numerical model and model parameters. For basement excavated at a side of an existing tunnel, the maximum settlement and horizontal displacement of the tunnel are always observed at the tunnel springline closer to basement and tunnel crown, respectively, regardless of basement geometry. By increasing basement length and width by five times, the maximum movements of tunnel located at the side of basement and directly underneath the basement increase by 450% and 186%, respectively. Obviously, tunnel movements are more sensitive to basement length rather than basement width. For basement excavated at a side of an existing tunnel, tunnel movements at basement centerline become stable when basement length reaches 10 H_e (i.e., final excavation depth). Moreover, tunnel heaves due to overlying basement excavation become stable when the normalized basement length (L/H_e) is larger than 8.0. As tunnel cover to diameter ratio varies from 2.5 to 3.0, the maximum heave and tensile strain of tunnel due to overlying basement excavation decrease by up to 41.0% and 44.5%, respectively. If basement length is less than 8 H_e, the assumption of plane strain condition of basement-tunnel interaction grossly overestimates tunnel movements, and ignores tensile strain of tunnel along its longitudinal direction. Thus, three-dimensional numerical analyses are required to obtain a reasonable estimation of tunnel responses due to adjacent and overlying basement excavations in clay.

• Geomechanics and Engineering
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• v.35 no.4
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• pp.449-464
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• 2023

Advanced nonlinear effective stress constitutive models are started to be frequently used in one-dimensional (1D) and two-dimensional (2D) site response analysis for assessment of porewater generation and liquefaction potential in soft soil deposits. The emphasis of this research is on the assessment of the implementation of this category of models at the element stage. Initially, the performance of a coupled porewater pressure (PWP) and constitutive models were evaluated employing a catalogue of 40 unidirectional cyclic simple shear tests with a variety of relative densities between 35% and 80% and effective vertical stresses between 40 and 80 kPa. The authors evaluated three coupled constitutive models (PDMY02, PM4SAND and PDMY03) using cyclic direct simple shear tests and for decide input parameters used in the model, procedures are recommended. The ability of the coupled model to capture dilation as strength is valuable because the studied models reasonably capture the cyclic performance noted in the experiments and should be utilized to conduct effective stress-based 1D and 2D site response analysis. Sandy soils may become softer and liquefy during earthquakes as a result of pore-water pressure (PWP) development, which may have an impact on seismic design and site response. The tested constitutive models are mathematically coupled with a cyclic strain-based PWP generation model and can capture small-strain stiffness and large-strain shear strength. Results show that there are minor discrepancies between measured and computed excess PWP ratios, indicating that the tested constitutive models provide reasonable estimations of PWP increase during cyclic shear (r_u) and the banana shape is reproduced in a proper way indicating that dilation and shear- strain behavior is well captured by the models.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.1
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• pp.217-229
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• 1994

A probabilistic method was employed to compare the prediction accuracy of axial and volumetric strains of Lade's double surface model with that of single surface model. Several experiments were conducted to examine the variabilities of soil parameters for two models using Back-ma river sand. Mean values and standard deviations of soil parameters obtained from experimental data were used for the evaluation of the uncertainty of analyzed strains by the first order approximation. It is shown that the variabilities of parameters in the single surface model are more consistent than those of the double surface model. However, in the accuracy of axial strain by probabilistic analysis, double surface model is more stable than single surface model. It is also shown that two models are excellent in view of the accuracy of the volumetric strain. The method given in this paper may be effectively utilized to estimate the constitutive model because other results of the comparison of two models coincide with those of this paper.

• Journal of the Korean Geosynthetics Society
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• v.14 no.2
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• pp.57-70
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• 2015

This paper presents the results of an investigation into the effect of forced temperature change cycles on physical and mechanical properties of sand and weathered granite soil. The effect of forced temperature change cylecs on the particle arrangement and the thermal conductivity was first investigated. A series of triaxial compression tests on the soils were also performed to look into the effect of temperature change cycles on the stress-strain-strength behavior. The results indicated that the forced temperature change cycle does not significantly affect the particle arrangement and thermal conductivity. It is shown however that the heating duration showed some effect on the deviatoric stress at failure while no significant effect due to the number of heating-cooling cycle was observed.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.66-81
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• 1989

The characteristics of resilient modulus(Mr) which dominates the life of pavement and the design of pavement were investigated on the test specimens which were cement treated and non-treated of the three different soil types. The results are summarized as follows : 1. The resilient modulus was decreased by increasing the cyclic deviator stress ($\sigma$d) , especially the resilient modulus was gradually decreased or sometimes increased when the value of ad was greater than 0.75- 1. 0kg/cm$^2$. 2. The resilient modulus was increased by increasing the homogeneous confined stress ($\sigma$do) and such phenomena were distinct on the coarse soils. 3. The resilient modulus was increased by increasing the ratio of confined stress(Kc), and this phenomena were eminent on the coarse soils too, and the higher permanent strain was showed by increasing the value of Kc. 4. In the drained cyclic triaxial compression test, the value of ad, Kc, and (Oho) was introduced by the following interrelated equations which were similar to the Mr model of Cole. Kcn/Mr=K1(J$_2$/ $\tau$oct)K2 ............. (coarse soli) Mcn/Mr=K3($\sigma$dp/ $\tau$f)k4 ...............(fine soils) 5. The stress path was not much affected by the value of Mr, however, moisture content, dry desity, and contant of fines affected the value of Mr. 6. In the soil-cement specimens, the resilient compression strain($\varepsilon$d) was decreased by the increment of the $\sigma$ho, and Mr was decreased by increasing the $\sigma$d 7. In the flexible pavement. the cement treated layer should be designed not to fail by the fatigue before the designed traffic load, and actually the pavement could cover the traffic load to a certain extent under the post-crack phase, therefore farther studies on this phenomena' are required in the design analysis. 8. The finite element computer program (ANALYS) was used for displacement analysis of pavement containing the cement-treated layer, The result showed that the program used for this analysis was proved to be usable.

• Applied Biological Chemistry
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• v.36 no.1
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• pp.11-16
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• 1993

A bacteria strain producing extracellular protein was isolated and identified from soil samples, and the optimum conditions of producing protein were investigated. Eleven strains of bacteria were isolated from soil samples. Among which WY-60 strain showed a very strong capability of producing protein and identified as a Bacillus sp. The optimum composition of nutrient medium for the production of the protein by WY-60 was fructose 4.0%, polypeptone 1.0%, $NH_4NO_3$ 0.1%, $K_2HPO_4$ 0.1%, $MgSO_4{\cdot}7H_2O$ 0.005%, $CaCO_3$ 1.0% and the optimum pH and temperature were 8.0 and $30^{\circ}C$, respectively. The penicillin G and lincomycin added to the above medium were effective for the protein production of the WY-60, but other antibiotics were non-effective. The maximum production of protein was obtained after 5 days culture.

• Journal of Microbiology and Biotechnology
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• v.23 no.11
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• pp.1598-1609
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• 2013

Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindane-contaminated environments.

• Journal of Soil and Groundwater Environment
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• v.6 no.1
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• pp.53-62
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• 2001

Effects of surface defect on thermal stability and stress crack resistance of high density polyethylene geomembranes in environmental conditions were examined by comparing the mechanical properties, chemical resistance and failure times of geomembranes between defective cases under different temperatures. Artificial surface defects were added to the surface of geomembranes by scratch apparatus designed specially. The number of surface defects was increased with the smaller size of scratch induced particles, and the more scratch addition numbers at the shear rate of scratch induced mechanism, 100mm/min. The tensile strength were decreased but the tensile strain was increased with the above conditions. In chemical resistance of defective geomembranes, the tensile strength were decreased but the tensile strain was increased with the longer immersion period and the higher temperature under the same scratch induced conditions. Finally, failure times of defective geomembranes by ESCR test were shifted to the shorter time ranges by increasing temperatures.

• Journal of the Korean Geotechnical Society
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• v.19 no.6
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• pp.7-14
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• 2003

In this study, undrained shear test was performed$K_o$ consolidation in order to study the shear strength characteristics of oysters-marine clay mixtures for three mixed ratios(0%, 25% and 50%). And, in order to study shear strength characteristics of oysters-marine clay mixtures, three different effective vertical stresses(200, 300 and 400kPa) were applied for the $K_o$ consolidation tests. In addition three different axial strain rates(0.005%/min, 0.05%/min, 0.5%/min) were applied for the case of effective vertical stress, 300kPa. According to experimental results, the more mixed ratios were increased, the more deviator stress was increased by crushing effect of oysters particles. especially, when effective vertical stress is 300kPa and mixed ratio increase from 25% to 50%, Test shows the increase of shear strength. But axial strain rate was not effect on the undrained shear strength. In the comparison and analysis that are based on the values of tests on the oysters-marine clay mixtures and the Mayne & Bishop's empiric formula, the undrained shear strength ratio shows a similar pattern of the tests. But for the prediction of the coefficient of the pore water pressure, the value of empiric formula shows more overestimated than the values of the tests at 0%, mixture ratio.