• Journal of Life Science
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• v.14 no.1
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• pp.193-197
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• 2004

A continuous enrichment culture procedure was used to isolate bacteria from various soil sources capable of suppressing large patch disease of turfgrass. Six isolates consistently suppressed large patch in turfgrass, and ranged in the spectrum of extracellular enzymes that they expressed. The best disease- suppressing isolate, TBM912, expressed protease, CMCase, and pectinase activity and inhibited the growth of Rhizectonin solani and Betrytis cinerea in vitro. Here we show that this strain also produces an antibiotic that was identified by TLC, SDS-PACE and HPLC analysis as lipopeptide.

• The Korean Journal of Food And Nutrition
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• v.13 no.2
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• pp.171-175
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• 2000

Microbial proteases have certain unique characteristics, and are now widely used in food, leather, detergent, and pharmaceutical industries. Thermophilic Actinomyces producing the protease was isolated from soil in Wonju city. This strain was able to grow and produce protease at the culture temperature of 50$^{\circ}C$. The maximum protease production was obtained when 0.5% soluble starch and 0.4% yeast extract were used as carbon and nitrogen source, respectively. The other culture condition for the maximal productivity of the protease was 0.1% K2HPO4, and 0.05% CaCl2 at initial pH 8.0 for 48 hours.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.130.2-131
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• 2000

No Abstract, See Full Text

• Journal of the Korean Geotechnical Society
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• v.20 no.8
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• pp.147-158
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• 2004

Laboratory dynamic tests are carried out to assess the liquefaction potential of saturated sands in most countries. However, simple results such as the maximum cyclic shear stress and the number of cycles at initial liquefaction are used in the experimental assessment of liquefaction potential, even though various results can be obtained from the dynamic test. In addition, it seemed to be inefficient because more than three dynamic tests with different stress ratio have to be carried out to draw a liquefaction resistance experimental curve. To improve the present assessment method fur liquefaction potential, a new critical resistible characteristic far soil liquefaction is proposed and verified through conventional cyclic triaxial tests with Jumunjin sand. In the proposed method, various experimental data such as effective stress path, stress-strain relationship, and the change of excess pore water pressure can be used in the determination of cumulative plastic shear strains at every 1/4 cycle. Especially, the critical cumulative plastic shear strain to initiate liquefaction can be defined in a specific point called a phase change point in the effective stress path and it can be calculated from a hysteric curve of stress-strain relationship up to this point. Through this research, it is found that the proposed cumulative plastic shear strain can express the dissipated energy to resist dynamic loads and consider the realistic soil dynamic behavior of saturated sands reasonably. It is also found that the critical plastic shear strain can be used as a registible index of soils to represent the critical soil dynamic state, because it seems to include no effect of large deformation.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.1
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• pp.53-59
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• 1990

The antagonistic fluorescent pseudomonas, which was isolated from continuous cropping rhizosphere of pepper and cucumber, was identified as Pseudomonas fluorescens (P.f.). For further study, transformant was derived from the isolated P.f. after spontaneous mutation to give antibiotic resistance to nalidixic acid and rifampicin as marked strain. Both P.f. and transformant strains were used for this study and the results obtained were summarized as follows. 1. One of the most effective antagonistic strain, KR164, was selected against F. solani, F. oxysporum, R. solani and this strain was identified and classified as Pseudomonas fluorescens biotype IV. 2. Transformant, KR1641, was derived from strain KR164 and both strains had the same biological and biochemical characteristics. 3, Mycelial lysis and abnormal mycelia of plant pathogenic fungi were microscopically observed after simultaneous culture of fungus and given bacterial strain. 4. The length of chinese cabbage to the autolyzed became longer with given bacterial strain in dark culture. 5. Percentage of germination, number of leaves, length of height, and length of root in chinese cabbage in pot experiment were improved by inoculation of given bacterial strain. 6. The number of given bacterial strain kept generally stable until 34 days after inoculation of itself in pot experiment. Inoculation of given bacterial strain did affect the number of plant disease fungi to be decreased but did not affect the number of other bacteria, Bacillus, in pot experiment.

• Journal of the Korean Geotechnical Society
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• v.28 no.3
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• pp.45-54
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• 2012

Various geosynthetics are widely applied to civil structures and waste landfill site for reinforcement and water resistance. The use of geosynthetics inevitably involves the coupled behaviors of different materials which include large displacement and strain-softening behaviors, etc. In this study, the effect of chemical element in the leachate on the interface shear strength under the cyclic loading condition was analyzed. The Multi-purpose Interface Apparatus (M-PIA) has been modified and cyclic direct shear tests have been performed. The submerging period of each specimen is 200 days. Additionally, the Field-Emission Scanning Electronic Microscopy (FIB) analysis has been also performed to induce the reason of the variation of disturbance function and verify the hypothesis on the decay-proof ability of geosynthetics. Consequently, the charateristics of chemical degradation of geosynthetic-soil interface are verified and the variation of the disturbance function is mainly caused by the different type of soil mineral decay, based on the FIB results.

• Journal of the Korean Geosynthetics Society
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• v.8 no.3
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• pp.25-34
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• 2009

Determination of design parameters of composite ground including colluvial soil layer is far difficult because the maximum particle size of such a soil is remarkably large and particle distribution may vary from area to area. The stress-strain behavior of colluvial soils is in fact dependent upon the engineering characteristics at the boundary between coarse and fine materials. However, strength parameters are in general determined based on the characteristics of fine material, which causes an underestimation of such parameters. In this study, strength parameters of colluvial soil are evaluated by means of BIMROCK model curve. In addition, limit equilibrium analysis is carried out to verify the rational shear strength evaluation.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.4
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• pp.1337-1347
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• 2013

The parametric analysis on ultimate behavior of buried cylindrical GFRP(Glass Fiber Reinforced Polymer) septic tank was presented. Two kinds of F.E. analysis model(soil-spring model and 3D full model) was constructed. The ultimate behavior of septic tank was investigated according to the size of stiffened steel ring and properties of underground soil. Ramberg-Osgood model and Druker-Prager model were used for material nonlinear characteristics of GFRP septic tank and soil, respectively. The diameter and thickness of stiffened steel ring inside septic tank, elastic modulus and internal friction angle of soil were selected for parametric variables. The ultimate behavior of septic tank, load-displacement, axial and hoop strain, were calculated and investigated.

• Journal of the Korean Geosynthetics Society
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• v.18 no.2
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• pp.11-21
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• 2019

This paper describes the applicability of FEA(Finite Element Analysis) to the simulation of pile pullout behavior under various soil conditions (relative density and fines content), in order to evaluate reasonably the pullout resistance of pile. That is, the results of previous research (You et al., 2018) were analyzed by FEA under the same conditions. The FEA results showed that axisymmetric analysis using virtual ground was able to evaluate the skin friction of the pile. Also, axisymmetric analysis, which can apply the shear resistance characteristics of the pile-soil interface in various soil conditions, could be used as an analytical method that can simulate a reasonable pile pullout behavior. Therefore, the analytical model proposed in this study was able to simulate appropriately the pullout behavior based on the stress-strain relationship of the pile-soil interface.

• Journal of the Korean Geotechnical Society
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• v.24 no.8
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• pp.149-160
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• 2008

Offshore pile foundations are prone to lateral soil pressures resulting from embankment construction for the reclamation on deepwater soft clay. Since the 1990s, offshore reclamation has actively progressed in Korea, connecting with the development of Songdo newtown, Incheon newport, and Busan newport representatively. Special attention has been given to lateral soil-structure interaction problems related to passively-loaded offshore pile foundations. Based on a plane strain large deformation finite element (LDFE) approach, this paper presents the results of investigation into undrained (short-term) and drained (long-term) behavior of passively-loaded offshore pile foundations. This study examines the effects of major factors, such as soil profile, pile head boundary condition, magnitude of embankment load, and average degree of consolidation. The results allowed quantification of differences in the magnitude of lateral soil pressure acting on the piles between undrained and drained phases.