• The Plant Pathology Journal
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• v.37 no.6
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• pp.662-672
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• 2021

Plant growth-promoting bacteria improve plant growth under abiotic stress conditions. However, their effects on microbial succession in the rhizosphere are poorly understood. In this study, the inoculants of Bacillus mesonae strain H20-5 were administered to tomato plants grown in soils with different salinity levels (EC of 2, 4, and 6 dS/m). The bacterial communities in the bulk and rhizosphere soils were examined 14 days after H20-5 treatment using Illumina MiSeq sequencing of the bacterial 16S rRNA gene. Although the abundance of H20-5 rapidly decreased in the bulk and rhizosphere soils, a shift in the bacterial community was observed following H20-5 treatment. The variation in bacterial communities due to H20-5 treatment was higher in the rhizosphere than in the bulk soils. Additionally, the bacterial species richness and diversity were greater in the H20-5 treated rhizosphere than in the control. The composition and structure of the bacterial communities varied with soil salinity levels, and those in the H20-5 treated rhizosphere soil were clustered. The members of Actinobacteria genera, including Kineosporia, Virgisporangium, Actinoplanes, Gaiella, Blastococcus, and Solirubrobacter, were enriched in the H20-5 treated rhizosphere soils. The microbial co-occurrence network of the bacterial community in the H20-5 treated rhizosphere soils had more modules and keystone taxa compared to the control. These findings revealed that the strain H20-5 induced systemic tolerance in tomato plants and influenced the diversity, composition, structure, and network of bacterial communities. The bacterial community in the H20-5 treated rhizosphere soils also appeared to be relatively stable to soil salinity changes.

• Molecules and Cells
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• v.45 no.7
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• pp.502-511
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• 2022

Bacterial volatile compounds (BVCs) exert beneficial effects on plant protection both directly and indirectly. Although BVCs have been detected in vitro, their detection in situ remains challenging. The purpose of this study was to investigate the possibility of BVCs detection under in situ condition and estimate the potentials of in situ BVC to plants at below detection limit. We developed a method for detecting BVCs released by the soil bacteria Bacillus velezensis strain GB03 and Streptomyces griseus strain S4-7 in situ using solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS). Additionally, we evaluated the BVC detection limit in the rhizosphere and induction of systemic immune response in tomato plants grown in the greenhouse. Two signature BVCs, 2-nonanone and caryolan-1-ol, of GB03 and S4-7 respectively were successfully detected using the soil-vial system. However, these BVCs could not be detected in the rhizosphere pretreated with strains GB03 and S4-7. The detection limit of 2-nonanone in the tomato rhizosphere was 1 µM. Unexpectedly, drench application of 2-nonanone at 10 nM concentration, which is below its detection limit, protected tomato seedlings against Pseudomonas syringae pv. tomato. Our finding highlights that BVCs, including 2-nonanone, released by a soil bacterium are functional even when present at a concentration below the detection limit of SPME-GC-MS.

• Journal of the Korean Geotechnical Society
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• v.22 no.8
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• pp.33-42
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• 2006

Geosynthetics reinforced soil (GRS) walls with a flexible wall face allow deformation. GRS walls constructed on the weak ground change in both horizontal earth pressures on wall faces and the tensile stress of geosynthetics, affecting the backfill in time until the deformation of the backfill and the foundation is completed. However, there are few studies that were done to measure and analyze the horizontal earth pressures and geosynthetics deformation on GRS walls constructed on the soft ground for a long period of time. Two field GRS walls in this study are constructed on a shallow layer of a weak foundation to measure and analyze geostynthetics deformation, horizontal earth pressures, and pore water pressures for the duration of approximately 16 months. Strain gauges are used to measure geosynthetics deformation; this study specifically suggests a new method of measuring nonwoven geotextile using strain gauges. Most geosynthetics deformation occurred within a month after the construction of GRS walls. The maximum deformation measured for approximately 16 months appeared as follows: nowoven geotextile: 6.05%, woven geotextile: 2.92%, and geogrid: 2.33%. Pore water pressures on the GRS wall can be ignored; however, horizontal earth pressures on the bottom and the upper part of the wall face appear larger than earth pressures at rest.

• Geomechanics and Engineering
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• v.33 no.5
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• pp.477-486
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• 2023

In the studies on fault dislocation of tunnel, existing literatures are mainly focused on the problems caused by normal and reverse faults, but few on strike-slip faults. The paper aims to research the deformation and failure mechanism of a tunnel under strike-slip faulting based on a model test and test-calibrated numerical simulation. A potential faulting hazard condition is considered for a real water tunnel in central Yunnan, China. Based on the faulting hazard to tunnel, laboratory model tests were conducted with a test apparatus that specially designed for strike-slip faults. Then, to verify the results obtained from the model test, a finite element model was built. By comparison, the numerical results agree with tested ones well. The results indicated that most of the shear deformation and damage would appear within fault fracture zone. The tunnel exhibited a horizontal S-shaped deformation profile under strike-slip faulting. The side walls of the tunnel mainly experience tension and compression strain state, while the roof and floor of the tunnel would be in a shear state. Circular cracks on tunnel near fault fracture zone were more significant owing to shear effects of strike-slip faulting, while the longitudinal cracks occurred at the hanging wall.

• Journal of the Korean Geotechnical Society
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• v.20 no.5
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• pp.145-159
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• 2004

The conventional method for assessment of liquefaction potential proposed by Seed and Idriss has been widely used in most countries because of simplicity of tests. Even though various data such as stress, strain, stress path, and excess pore water pressure can be obtained from the dynamic test, especially, two simple experimental data such as the maximum deviatoric stress and the number of cycles at liquefaction have been used in the conventional assessment. In this study, a new detailed assessment for liquefaction potential to reflect both characteristics of real earthquake motion and dynamic soil resistance is proposed and verified. In the assessment, the safety factor of the liquefaction potential at a given depth of a site can be obtained by the ratio of a resistible cumulative plastic shear strain determined through the performance of the conventional cyclic test and a driving cumulative plastic shear strain calculated from the shear strain time history through the ground response analysis. The last point to cumulate the driving plastic shear strain to initiate soil liquefaction is important for this assessment. From the result of cyclic triaxial test using real earthquake motions, it was concluded that liquefaction under the impact-type earthquake loads would initiate as soon as a peak loading signal was reached. The driving cumulative plastic shear strain, therefore, can be determined by adding all plastic shear strains obtained from the ground response analysis up to the peak point. Through the verification of the proposed assessment, it can be concluded that the proposed assessment for liquefaction potential can be a progressive method to reflect both characteristics of the unique soil resistance and earthquake parameters such as peak earthquake signal, significant duration time, earthquake loading type, and magnitude.

• KSBB Journal
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• v.17 no.3
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• pp.247-254
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• 2002

Two kinds of crude oil-degrading microorganisms from soil and one kind from sea were isolated and named strain Al32, strain F722 and strain OM1, respectively. These microorganism were identified Acinetobacter sp., Pseudomonas aeruginosa and Acinetobacter calcoaceticus, respectively. The optimum cultivation temperature of Acinetobacter sp. A132 and P. aeruginosa F722 was $35^{\circ}C$ and optimum growth pH was 8 and 9, respectively. The growth was the highest at 2.0% (w/v) substrate concentration when crude oil was only carbon source. The growth of A. calcoaceticus OM1 isolated from sea was the highest at 3.0% (w/v) of crude oil. In inspection of crude oil degradability, strain Al32 showed 5.49 g/L.day with Eleuthera (OMAN), 2.0% (w/v). P. aeruginosa F722 showed 1.19 g/L g/L.day with L-Zakum (AFRICA). In case of kerosene $nC_9\simnC_{20}$ and diesel $nC_9\simnC_{28}$, A. calcoaceticus OM1 was degraded 95% and 75%, respectively, for 7 days culture, and P. aeruginosa F722 was 80% after 10 days.

• Molecules and Cells
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• v.28 no.3
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• pp.209-213
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• 2009

Caenorhabditis elegans is a free living soil nematode and thus in its natural habitat, C. elegans encounters many different species of soil bacteria. Although some soil bacteria may be excellent sources of nutrition for the worm, others may be pathogenic. Thus, we undertook a study to understand how C. elegans can identify their preferred food using a simple behavioral assay. We found that there are various species of soil bacteria that C. elegans prefers in comparison to the standard laboratory E. coli strain OP50. In particular, two bacterial strains, Bacillus mycoides and Bacillus soli, were preferred strains. Interestingly, the sole feeding of these bacteria to wild type animals results in extended lifespan through the activation of the autophagic process. Further studies will be required to understand the precise mechanism controlling the behavior of identification and selection of food in C. elegans.

• Geotechnical Engineering
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• v.11 no.3
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• pp.43-54
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• 1995

The aim of the work described in this paper is to develope a constitutive model for the prediction of an unsaturated soil and to confirm the application'of the model, which is composed of the elastic and plastic part in consideration of the matric suction and the net mean stress. From test results, volume changes and deviator stresses are analyzed at each state and their relationships are formulated. The application of the model to silty sands is confirmed by the comparison between test and predicted results. During drying -wetting and loading -unloading processes for isotropic states, the agreement between predicted and test results are satisfactory. Predicted deviator stresses are well agreed with test results in shearing process. Overall acceptable predictions are reproduced in high confining pressure. Usefulness of the model is confirmed for the unsaturated soil except volumetric strain, which is not well agreed with the test results due to deficiency of dilatancy of the model in low confining pressure. It is, therefore. recommended to study the behavior of dilatancy for an unsaturated soil.

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.36-42
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• 1995

Actinomycetes occur in a wide range of environments and many more actinomycetes remain to be detected in the natural environment. In isolation stages, selection of the environment as a source of useful isolates is important. Two hundred and eighteen strains were isolated on Bennet's agar and 346 strains were on humic acid-vitamin agar from five each paddy field, field, forest, grass land, riverside soil samples. These isolates were identified to the genus level based on morphological and physiological characteristics. Among them, 386 strains were Streptomyces, 49 strain were Nocardia, 35 strains were Microbispora and Micromonospora each, 15 strains were Nocardiopsis, 13 strains were Actinomadura, 10 strains were Streptosporangium, and the others were isolated rarely. According to soil type, Nocardia, Micromonospora, Microbispora and Streptosporangium were dominant in paddy field, Microbispora Nocardia, Nocardioides and Micromonospora were dominant in field, Nocardia, Micromonospora, Microbispora and Actinomadura were dominant in grass land, Nocardia, Micromonospora and Microbispora were dominant in forest, Nocardia, Microbispora and Micromonospora were dominant in riverside. Generally, Nocardia, Micromonospora, Microbispora and Actinomadura were isolated in all kinds of soils, Streptosporangium were paddy field, Dactylosporangium were forest, Nocardiopsis were field, forest and riverside.

• Geomechanics and Engineering
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• v.8 no.6
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• pp.829-844
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• 2015

In the current paper the results of a numerical simulation that were verified by a well instrumented experimental procedure for studying the arching effect over a trapdoor in sand is presented. To simulate this phenomenon with continuum mechanics, the experimental procedure is modeled in ABAQUS code using stress dependent hardening in elastic state and plastic strain dependent frictional hardening-softening with Mohr Coulomb failure criterion applying user sub-routine. The apparatus comprises rectangular trapdoors with different width that can yield downward while stresses and deformations are recorded simultaneously. As the trapdoor starts to yield, the whole soil mass deforms elastically. However, after an immediate specified displacement, depending on the width of the trapdoor, the soil mass behaves plastically. This behavior of sand occurs due to the flow phenomenon and continues until the stress on trapdoor is minimized. Then the failure process develops in sand and the measured stress on the trapdoor shows an ascending trend. This indicates gradual separation of the yielding mass from the whole soil body. Finally, the flow process leads to establish a stable vault of sand called arching mechanism or progressive collapse of the soil body.