• Mycobiology
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• v.51 no.2
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• pp.72-78
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• 2023

In this study, a fungal strain KNUF-22-025 belonging to the genus Botryotrichum was isolated from the soil in Korea. The cultural and morphological characteristics of this strain differed from those of closely related species. On malt extract agar, strain KNUF-22-025 showed slower growth than most of the related species, except B. domesticum. The conidia size (9.6-21.1×9.9-18.4 ㎛) of strain KNUF-22-025 was larger than those of B. piluliferum, B. domesticum, and B. peruvianum but smaller than those of B. atrogriseum and B. iranicum. Conidiophores in strain KNUF-22-025 (137 ㎛) were longer than those in other closely related species but shorter than those in B. atrogriseum. Multi-locus analysis of molecular markers, such as ITS, 28S ribosomal DNA, RBP2, and TUB2 revealed that strain KNUF-22-025 was distinct from other Botryotrichum species. Thus, this strain is proposed as a novel species based on morphological characteristics along with molecular phylogeny and named Botryotrichum luteum sp. nov.

• Structural Monitoring and Maintenance
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• v.10 no.2
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• pp.155-174
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• 2023

High performance concrete (HPC) has been extensively used in thin overlay for repair purpose due to its excellent strength and durability. This paper presents an experiment, where the sensor-instrumented HPC overlays have been followed by dynamic strain and moisture content monitoring for 1 year, under normal traffic. The vibrating wire and soil moisture sensors were embedded in overlay before construction. Four given HPC mixes (2 original mixes and their shrinkage-modified mixes) were used for overlays to contrast the strain and moisture results. A calibration method to accurately measure the moisture content for a given concrete mixture using soil moisture sensor was established. The monitoring results indicated that the modified mixes performed much better than the original mixes in shrinkage cracking control. Weather condition and concrete maturity at early age greatly affected the strain in concrete. The strain in HPC overlay was primarily in longitudinal direction, leading to transverse cracks. Additionally, the most moisture loss in concrete occurred at early age. Its rate was very dependent on weather. After one year, cracking survey was carried out by vision to verify the strain direction and no cracks observed in shrinkage modified mixes.

• Journal of Microbiology and Biotechnology
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• v.34 no.9
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• pp.1819-1825
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• 2024

An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6^T was isolated from soil in Republic of Korea. Strain WR6^T grew at temperatures of 10-37℃, at pH of 5.0-9.0, and at NaCl concentrations of 0-3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6^T affiliated to the genus Mesorhizobium, with the nearest relative being Mesorhizobium waimense ICMP 19557^T (98.5%). The genome of strain WR6^T was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6^T, Q-10 was sole ubiquinone; summed feature 8 (C_18:1ω7c and/or C_18:1ω6c) and C_19:0 cyclo ω8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6^T represents a novel species in the genus Mesorhizobium. Accordingly, we propose the name Mesorhizobium koreense sp. nov., with the type strain WR6^T (=KCTC 92695^T =NBRC 116021^T).

• KSCE Journal of Civil and Environmental Engineering Research
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• v.10 no.2
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• pp.91-102
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• 1990

The representative tests in this study are performed at a selected site which has the soil layers to analyze the safety and economy of the dynamic analysis for the variable soil conditions. Crosshole test and downhole test of small strain level tests and triaxial test of large strain level test are performed in the soil layers, and in the rock layers, crosshole test and downhole in-situ tests and laboratory sonic test are performed to measure the dynamic shear modulus, damping ratio, and Poisson$\acute{s}$ ratio of the soil and the rock. The correlations between the dynamic soil properties from the tests and the basic soil properties are determined through the regression analysis. The representative design value of the soil is determined by probability analysis of the test results. It is determined from the nonlinear stress-strain model in soils, and the value at small strain level is computed in rocks according to the distribution of the type of soils and the affecting variables. The constitutive value is systematized to be utilized in the analysis of the test results, and computation of the input soil data.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.2
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• pp.97-103
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• 2008

Determination of stress-strain relationship in torsional tests is complicated due to nonuniform stress-strain variation occurring linearly with the radius in a soil specimen in torsion. The equivalent radius approach is adequate when calculating strain at low to intermediate strains, however, the approach is less accurate when performing the test at higher strain levels. The modified equivalent radius approach was developed to account for the problem more precisely. This approach was extended to generate the plots of equivalent radius ratio versus strain using modified hyperbolic and Ramberg-Osgood models. Results showed the effects of soil nonlinearity on the equivalent radius ratio curves were observed. Curve fitting was also performed to find the stress-strain relationship by fitting the theoretical torque-rotation relationship to measured torque-rotation relationship.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.602-608
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• 2010

An atrazine-degrading bacterium, strain KU001, was obtained from a sugarcane field at the Cane and Sugar Research and Development Center at the Kasetsart University, Kamphaeng Saen Campus, Thailand. Strain KU001 had a rod-to-coccus morphological cycle during growth. Biolog carbon source analysis indicated that the isolated bacterium was Arthrobacter histidinolovorans. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain KU001 was 99% identical to the same region in Arthrobacter sp. The atrazine degradation pathway in strain KU001 consisted of the catabolic genes trzN, atzB, and atzC. Strain KU001 was able to use atrazine as a sole nitrogen source for growth, and surprisingly, atrazine degradation was not inhibited in cells grown on ammonium, nitrate, or urea, as compared with cells cultivated on growth-limiting nitrogen sources. During the atrazine degradation process, the supplementation of nitrate completely inhibited atrazine degradation activity in strain KU001, whereas ammonium and urea had no effect on atrazine degradation activity. The addition of strain KU001 to sterile or nonsterile soils resulted in the disappearance of atrazine at a rate that was 4- to 5-fold more than that achieved by the indigenous microbial community. The addition of citrate to soils resulted in enhanced atrazine degradation, where 80% of atrazine disappeared within one day following nutrient supplementation.

• Journal of Species Research
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• v.10 no.4
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• pp.336-343
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• 2021

In 2020, as a subset study to discover indigenous yeast species in Korea, a total of 22 yeast species were isolated from soil samples collected in Gwangju-si, Gyeonggi-do. Among them, 10 strains were unreported species. From the high 26S rRNA gene sequence similarity and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged independent and predefined yeast species. The 22 strains were assigned to the genera Dothiora (1 strain), Sarocladium (1 strain), Tetrapisispora (1 strain) and Torulaspora (2 strains) of the phylum Ascomycota; the genera Erythrobasidium (1 strain), Leucosporidium (4 strains), Ustilentyloma (2 strains), Fellozyma (1 strain), Sampaiozyma (2 strains), Filobasidium (1 strain), Solicoccozyma (2 strains) and Vishniacozyma (4 strains) of the phylum Basidiomycota. This is the first official report of the following species in Korea: Dothiora cannabinae (1 strain), Sarocladium strictum (1 strain), Fellozyma inositophila (1 strain), Filobasidium magnum (1 strain), Solicoccozyma phenolicus (1 strain), Solicoccozyma terreus(1 strain), Vishniacozyma tephrensis(1 strain) and Vishniacozyma victoriae (3 strains). Cell morphology, phenotypic features and biochemical features are described in the Species Description section.

• Journal of the Korean GEO-environmental Society
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• v.16 no.10
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• pp.5-14
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• 2015

Suction affects the unsaturated soil as the negative pore pressure, and leads to increases of the yield stress and the plastic shear stiffness of the soil skeleton due to the growth in interparticle stress. Hence, in this study, in order to account for these effects of suction under the dynamic loading condition such as the earthquake, the element simulation of the cyclic triaxial test using induced stress-strain relation based on cyclic elasto-plastic constitutive model extended for unsaturated soil considering the $1^{st}$ and the $2^{nd}$ yield functions was conducted. Through the stress path, stress-strain relation and relation between volumetric strain and axial strain, it was seen in all the cases that the simulation results demonstrated a good agreement with the experimental results. It is expected that the results of this study possibly contribute to the accuracy improvement on the prediction of unsaturated soil behavior under the dynamic loading condition.

• Journal of the Korean Geotechnical Society
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• v.17 no.6
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• pp.85-98
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• 2001

Cone penetration was analyzed by arbitrary Lagangian-Eulerian(ALE) method. In order to simulate full penetration, steady state analyses were performed using ABAQUS/Explicit, which models upward flow of soil layers. In the analysis of homogeneous layer it was found that the paths and the strain of soil particles were consistent with the result of the strain path method and that the ultimate resistance were reasonably evaluated. The cone penetration through different soil layers was also analyzed and that showed the transfer of cone resistance. The steady state ALE analysis could perform full penetration through the layered soils.

• Geomechanics and Engineering
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• v.34 no.4
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• pp.409-424
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• 2023

Response of the pipeline crossing fault is considered as the large strain problem. Proper estimation of the pipeline response plays important role in mitigation studies. In this study, an advanced continuum modeling including material non-linearity in large strain deformations, hardening/softening soil behavior and soil-pipeline interaction is applied. Through the application of a fully nonlinear analysis based on an explicit finite difference method, the mechanics of the pipeline behavior and its interaction with soil under large strains is presented in more detail. To make the results useful in oil and gas engineering works, a continuous pipeline of two steel grades buried in two clayey soil types with four different crossing angles of 30°, 45°, 70° and 90° with respect to the pipeline axis have been considered. The results are presented as the fault movement corresponding to different damage limit states. It was seen that the maximum affected pipeline length is about 20 meters for the studied conditions. Also, the affected length around the fault cutting plane is asymmetric with about 35% and 65% at the fault moving and stationary block, respectively. Local buckling is the dominant damage state for greater crossing angle of 90° with the fault displacement varying from 0.4 m to 0.55 m. While the tensile strain limit is the main damage state at the crossing angles of 70° and 45°, the cross-sectional flattening limit becomes the main damage state at the smaller 30° crossing angles. Compared to the stiff clayey soil, the fault movement resulting 3% tensile strain limit reach up to 40% in soft clayey soil. Also, it was seen that the effect of the pipeline internal pressure reaches up to about 40% compared to non-pressurized condition for some cases.