• Title/Summary/Keyword: soil strain

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Biochemical and molecular characterization of a strain KA/S2 of Acnnthamoebc castellanii isolated from Korean soil (카스텔란니가시아메바(Acanthamoeba castellanii) 한국 토양분리주 KA/S2의 생화학적 및 분자생물학적 특성)

  • 정동일;공현희
    • Parasites, Hosts and Diseases
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    • v.34 no.1
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    • pp.79-86
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    • 1996
  • A strain, KA/S2, isolated from Korean soil and morphologically assigned to Acanthcmoebc cQsteLlcnii, was characterized by isoenzyme analysis , and total proteins profile, End mitochondrial (Mt) DNA restriction fragment length polymorphism (RFLP) , and compared with four reference strains assigned to the species (the authenitic Castellani, Neff, Ma, and Chang strains). It was found that four isoenzyme, total proteins, and Mt DNA RFLP patterns by eight restriction endonucleases of the strain KA/S2 were identical with those of the Neff strain, isolated from soil of California, USA. The Chang strain was unique in its morphology and total protein patterns. Interstrain polymorphisms of isoensyme profiles and Mt DNA RFLP patterns were observed among the Castellani, Neff, Ma, and Chang strains. Mt DNA RFLP was confirmed to be highly appropriate for the strain characterization and identification of Acnnthamoeba spry.

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A Study on Load Transfer between Soil and Nail Using In-situ Pull-out Tests (현장인발시험을 통한 흙-네일의 하중 전이특성에 대한 연구)

  • Kim, Jong-Soo;Yi, Chang-Tok;Min, Kyong-Jun;Lee, Song
    • Proceedings of the Korean Geotechical Society Conference
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    • 1999.03a
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    • pp.167-174
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    • 1999
  • A Land slide in Granitic Gneiss weathered soil was stabilized successfully with soil nailing using 929mm steel bar. To understand the behavior of load transfer between soil and nail, in-situ pdl-out tests were carried out. The strains of steel bars were measured using strain gauges during pull-out tests. Forces-strain data from laboratory tension tests on steel bar and grouted steel bar were examined to compare with those of the pull-out tests. Comparisons were made between the pull-out test results and laboratory test result to understand load transfer mechanism.

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The Analysis of Soil Behaviour by Double Surface Work-hardening Constitutive Model (복합항복면 일-경화구성 모델을 이용한 지반거동해석)

  • Youn, Il-Ro;Oh, Se-Wook
    • Journal of the Korean Society of Industry Convergence
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    • v.15 no.1
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    • pp.21-27
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    • 2012
  • Decomposed granite soils are in a wide range of conditions depending on the degrees of weathering. This paper is intended to examine laboratory tests such as consolidation tests and conventional triaxial compression tests conducted in order to find out the mechanical properties of Cheongju granite soil. Along with the foregoing, the results of basic physical tests conducted in order to grasp the physical properties of Cheongju granite soil were described and based on the results, methods to calculate the mechanical parameters of numerical approaches using Lade's double surface work-hardening constitutive model were examined. Finally, it is intended to explain the stress properties of Cheongju granite soil used as a geotechnical material based on its shear behavior and critical state concept using the results of isotropic consolidation tests and triaxial compression tests. As a conclusion, it can be seen that in the relationship between confining stress and maximum deviator stress, the slope is maintained at a constant value of 2.95. In the drained CTC test, maximum deviator stress generally existed in a range of axial strain of 6~8% and larger dilatancy phenomena appeared when confining stress was smaller. Finally, based on the results of the CTC tests on Cheongju granite soil, although axial strain, deviator stress and pore water pressure showed mechanical properties similar to those of overconsolidated soil, Cheongju granite soil showed behavior similar to that of normally consolidated soil in terms of volumetric strain.

Assessment Method of Geosynthetic Pullout Resistance Considering Soil Confinement Effect (구속효과를 고려한 토목섬유의 인발저항력 평가기법)

  • 방윤경;이준대;전영근
    • Journal of the Korean Geotechnical Society
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    • v.17 no.6
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    • pp.135-148
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    • 2001
  • In this study, an assessment method was proposed to evaluate the pullout resistance between geosynthetic and backill soil by using a stress-strain relationship of the orthotropic composite material subjected to both longitudinal and vertical loadings. For this analysis friction characteristics of geosynthetic-soil and stress-strain relationships subjected to soil confined pressure were investigated by performing the laboratory pullout tests for three types of geosynthetics and performing the confined extension tests far seven types of geosynthetics having geotextiles, composite geosynthetics and geogrids. A comparison was made between unconfined an confined moduli far each geosynthetic material to quantify the soil confinement effect on stress-strain properties. A comparison was also made between the relative increase of moduli at the same strain level among the seven geosynthetic materials to demonstrate the different responses of these geosynthetic materials under soil confinement. Based on the proposed procedure, it was shown that values of the increased tensile force are applicable fur the evaluation of friction strengths between five types of geosynthetics and sands in light of the soil confinement effect.

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BTEX Biodegradation in Contaminated Soil Samples Using Pure Isolates and Changes in the Mixed Microbial Community Structure (순수 분리 미생물을 이용한 오염 토양에서의 BTEX 생분해 특성과 미생물 군집 변화)

  • Chung, Kyung-Mi;Choi, Yong-Su;Hong, Seok-Won;Lee, Soo-Jin;Lee, Sang-Hyup
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.7
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    • pp.757-763
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    • 2006
  • In our previous studies, we have isolated bacteria from BTEX-contaminated sediment, which utilized BTEX as a sole carbon source and $NO_3$-N as an electron acceptor. For the possibility of field application, we have applied co-culture of those isolates in the BTEX-contaminated soil and evaluated their biodegradation efficiencies. To investigate the relationship between the isolates and indigenous microorganism in soil, changes of microbial community structure in soil samples with respect to time were monitored. To examine this, soil samples were artificially contaminated with benzene, toluene, ethylbenzene and o-xylene. BTEX-degrading bacteria such as Pseudomonas stutzeri strain 15(DQ 202712), Klebsiells sp. strain 20(DQ 202715) and Citrobacter sp. strain A(DQ 202713) were injected into the soil samples in the ratio of 2:1:1. Our results showed that the highest BTEX biodegradation efficiency was achieved when both BTEX and $NO_3-N$ existed simultaneously. The change in soil microbial community structure was characterized by PCR-DGGE analysis comparing the relative DGGE band intensities. The band intensities of indigenous microorganisms in the soil were reduced by injecting co-culture of the three isolates. On the contrary, the relative band intensities of the isolates were increased. Among the three isolates, Pseudomonas stutzeri strain 15 rendered the highest band intensity. This indicates that the Pseudomonas stutzeri was the dominant microbial species found in the soil samples.

An analytical investigation of soil disturbance due to sampling penetration

  • Diao, Hongguo;Wu, Yuedong;Liu, Jian;Luo, Ruping
    • Geomechanics and Engineering
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    • v.9 no.6
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    • pp.743-755
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    • 2015
  • It is well known that the quality of sample significantly determines the accuracy of soil parameters for laboratory testing. Although sampling disturbance has been studied over the last few decades, the theoretical investigation of soil disturbance due to sampling penetration has been rarely reported. In this paper, an analytical solution for estimating the soil disturbance due to sampling penetration was presented using cavity expansion method. Analytical results in several cases reveal that the soil at different location along the sample centerline experiences distinct phases of strain during the process of sampling penetration. The magnitude of induced strain is dependent on the position of the soil element within the sampler and the sampler geometry expressed as diameter-thickness ratio D/t and length-diameter ratio L/D. Effects of sampler features on soil disturbance were also studied. It is found that the induced maximum strain decreases exponentially with increasing diameter-thickness ratio, indicating that the sampling disturbance will reduce with increasing diameter or decreasing wall thickness of sampler. It is also found that a large length-diameter ratio does not necessarily reduce the disturbance. An optimal length-diameter ratio is suggested for the further design of improved sampler in this study.

Free-strain solutions for two-dimensional consolidation with sand blankets under multi-ramp loading

  • Zan Li;Songyu Liu;Cuiwei Fu
    • Geomechanics and Engineering
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    • v.35 no.4
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    • pp.385-393
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    • 2023
  • To analyze the consolidation with horizontal sand drains, the plane strain consolidation model under multi-ramp loading is established, and its corresponding analytical solution is derived by using the separation of variables method. The proposed solution is verified by the field measurement data and finite element results. Then, the effects of the loading mode and stress distribution on consolidation and dissipation of pore pressure are investigated. At the same time, the influence of hydraulic conductivity and thickness of sand blankets on soil consolidation are also analyzed. The results show that the loading mode has a significant effect on both the soil consolidation rate and generation-dissipation process of pore water pressure. In contrast, the influence of stress distribution on pore pressure dissipation is obvious, while its influence on soil consolidation rate is negligible. To guarantee the fully drained condition of the sand blanket, the ratio of hydraulic conductivity of the sand blanket to that of clay layer kd/kv should range from 1.0×104 to 1.0×106 with soil width varying from 100 m to 1000 m. A larger soil width correspondingly needs a greater value of kd/kv to make sure that the pore water can flow through the sand blanket smoothly with little resistance. When the soil width is relatively small (e.g., less than 100 m), the effect of thickness of the sand blanket on soil consolidation is insignificant. And its influence appears obvious gradually with the increase of the soil width.

Dynamic stress, strain and deflection analysis of pipes conveying nanofluid buried in the soil medium considering damping effects subjected to earthquake load

  • Abadi, M. Heydari Nosrat;Darvishi, H. Hassanpour;Nouri, A.R. Zamani
    • Computers and Concrete
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    • v.24 no.5
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    • pp.445-452
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    • 2019
  • In this paper, dynamic stress, strain and deflection analysis of concrete pipes conveying nanoparticles-water under the seismic load are studied. The pipe is buried in the soil which is modeled by spring and damper elements. The Navier-Stokes equation is used for obtaining the force induced by the fluid and the mixture rule is utilized for considering the effect of nanoparticles. Based on refined two variables shear deformation theory of shells, the pipe is simulated and the equations of motion are derived based on energy method. The Galerkin and Newmark methods are utilized for calculating the dynamic stress, strain and deflection of the concrete pipe. The influences of internal fluid, nanoparticles volume percent, soil medium and damping of it as well as length to diameter ratio of the pipe are shown on the dynamic stress, strain and displacement of the pipe. The results show that with enhancing the nanoparticles volume percent, the dynamic stress, strain and deflection decrease.

The Swelling Characteristics of Clayey Soil by CRS Consolidation Test (CRS 압밀시험에 의한 점성토의 팽창특성에 관한 연구)

  • 이응준;한상재;김지용;김수삼
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.11a
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    • pp.335-342
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    • 2000
  • In this study, the swelling characteristics of reconstituted clayey soil were investigated by STD and CRS test. The strain rate during loading was constant i.e. 0.05 %/min, 0.03 %/min and during unloading was varied in proportion to 1/1, 1/5, 1/10 and 1/15 of strain rate during loading. From this study the following conclusions were obtained; (1) There were similar values, especially, during unloading in case of 1/10 or 1/15 of strain rate during loading and the test results between STD and CRS were much to be alike. (2) The cross point of effective stress versus excess pore water pressure ratio curve, was increased during unloading, while the stress level of the cross point was decreased. The stress level can be separated into two zones according to the swelling index named Cs1 and Cs2. From the test results, the values of Cs1 were approximately constant irrespective of strain rate during unloading, but the values of Cs2 were much influenced by strain rate. (3) In CRS consolidation tests, it was found that unloading strain rate did not affect on the existence of zone.

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Studies on Antibiotic Producers of Korean Soil Microbes(I) -Isolation and Antibiotic Activity of Streptomyces Strain DMC-72- (한국(韓國) 토양균(土壤菌)중 항생물질(抗生物質) 생성균(生成菌)에 관한 연구(硏究)(제1보)(第1報) -스트렙토마이세스속(屬) 균주(菌株) DMC-72호(號)의 분리(分離) 및 항균작용(抗菌作用)-)

  • Kim, Kwang-Wook;Choi, Eung-Chil;Shim, Mi-Ja;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.15 no.1
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    • pp.15-23
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    • 1984
  • To find antimicrobial strains of the soil microorganisms in Korea, they were isolated from the soil samples of different locations and screened for antibiotic activity against several standard microbes. An isolate among them had an antibacterial activity against gram-positive bacteria. The examination of its morphological and biochemical characteristics according to the International Streptomyces Project methods showed that it belongs to the genus Streptomyces. The strain was named DMC-72. The strain appears to be a new strain when it was compared with the species within the genus which have been so far reported. The antibiotic metabolite of the strain was produced in submerged culture method. It was found to be a quinone compound and was named soulomycin. This strain was also found to produce an ${\alpha}-amylase$ inhibitor in the submerged culture.

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