• Title/Summary/Keyword: soil strain

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Isolation and Characterization of Streptomyces sp. KACC 91027 Against Plasmodiophora brassicae

  • Kim, Seung-Hyung;Shin, Choon-Shik;Moon, Sang-Ik;Yi, Young-Sub;Choi, Gyung-Ja;Cho, Kwang-Yun;Song, Jae-Kyeong;Lim, Yoon-Gho
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.220-223
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    • 2004
  • Club root caused by Plasmodiophora brassicae is found in crucifers. Among the over hundreds of Streptomyces isolated from soil in Korea. One strain showed prominent activity against P. brassicae. The strain was identified based on 16S rDNA sequencing and the morphology by a method of scanning electron microscopy. An active compound in the fermented broth obtained from the strain was separated. Even though the complete assignments of the compound remain for future work, the results regarding the isolation and characterization of the strain with a certain activity against P. brassicae are shown in this paper.

$\alpha$-D-Glucosidase Inhibitor from Streptomyces sp. (I) - Identification of the Strain - (Streptomyces속 균주가 생성하는 $\alpha$-D-Glucosidase Inhibitor( I ) -균주의 동정 -)

  • 도재호;주현규
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.202-206
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    • 1989
  • A microorganism, YS-221-B strain which powerfully produced $\alpha$-D-Glucosidase Inhibitor was isolated from soil. The morphological, cultural characteristics and physiological properties of the strain were investigated. As the results of various examinations, the strain YS-221-B was identified to be similar to Streptomyces flavouirens except the utilization of raffinose, i-inositol among the various carbon sources.

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Nematocidal Effect of B. thuringiensis subsp.indiana Strain BtTH109 on Root-Knot Nematode of Tomato (Bacillus thuringiensis subsp. indiana BtTH109의 토마토 뿌리혹선충에 대한 치사효과)

  • 이광배;김광현
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.679-684
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    • 1994
  • For a biological control of root-knot nematode (Meloidogyne incognita) in tomato, efficiency of Bacillus thuringiensis subsp. indiana strain TH109 (BtTH109) on the nematode control was investigated. After the mixture of strain BtTH109 and wheat bran was treated into rhizosphere of the tomato plants with nematode eggs, the stem height and root growth of plants increased. And the juveniles and eggs of nematode are not found in both roots of tomatoes and pot soil after cultured broth of the strain BtTH109 treated 4 times at 3 day-interval into rhizosphere of the infected tomatoes.

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Identification of Aspergillus Strain with Antifungal Activity Against Phytophthora Species

  • KANG SUNG WOO;HONG SUK IN;KIM SEUNG WOOK
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.227-233
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    • 2005
  • Fungal strain CGF was isolated from the soil of ChungNam Province, South Korea. Based on the 28S rDNA sequence analysis and the sequence of the internal transcribed spacer (ITS) region of ribosomal DNA, together with morphological and cultural characteristics, this strain was identified as Aspergillus sclerotiorum and renamed Aspergillus sclerotiorum CGF. This is the first strain of Aspergillus sclerotiorum identified in Korea. When the antifungal activity of A. sclerotiorum CGF was evaluated, among the phytopathogenic fungi, mycelial growth of only Phytophthora species was inhibited. Oermination of P. capsid zoospore was also inhibited. The bioactive compound of A. sclerotiorum CGF was highly thermo- and pH-stable.

Identification of the Streptomyces Strain HSL-613 Producing Cholesterol Oxidase (Cholesterol Oxidase를 생산하는 방선균분리주 HSL-613의 동정)

  • Lee, Hong-Soo;Lee, In-Ae;Choe, Yong-Kyung;Lee, Hee-Gu;Lee, Keun-Chul;Park, Yong-Ha;Oh, Tai-Kwang;Choe, In-Seong;Chung, Tai-Wha
    • Microbiology and Biotechnology Letters
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    • v.22 no.4
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    • pp.373-381
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    • 1994
  • An actinomycete strain, HSL-613 was isolated -from soil and identified by International Streptomyces Project (ISP) and chemotaxonomic methods. The spore chain of the strain HSL-613 appears in a spiral shape, and its spores are spherical shape with smooth surface. The cell wall contains LL-diaminopimelic acid (DAP). Menaquinone MK-9 (H$_{6}$, H$_{8}$) and iso- and anteiso-branched fatty acids were detected from whole cell extract. Sugars identified from whole cell extract include galactose, glucose, mannose and ribose, which are distinct from general sugar patterns of Streptomyces. Average G+C content in the chromosome is 59%. 5S rRNA of HSL-613 consists of 120 nucleotides as determined by comparing with that of a type strain Streptomyces griseus subsp. KCTC 9080. Through morphological, physiological, and chemical characterization, HSL-613 was identified and named as Streptomyces sp. HSL-613.

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Purification of Cholesterol Esterase from Aeromoans SP. (Aeromoans SP.가 생산하는 콜레스테롤 에스테라아제의 정제)

  • 박부길;이해익
    • KSBB Journal
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    • v.5 no.1
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    • pp.19-23
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    • 1990
  • A cholesterol esterase-producing microorganism, strain CES 506, isolated from soil was identified as Aeromonas sp. This strain produce about 0.023 units of cholesterol esterase per ml of culture broth. The cholesterol esterase produced by this strain was purified, 370 fold to homogeneity in an overall yield of 24% from culture broth. The apparent molecular weight was 64, 000, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme showed a high substrate specificity for cholesterylpalmitate and the Km value for the hydrolysis of cholesterylpalmitate by this enzyme were 0.15mM.

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Isolation and Identification of a Bacterium Producing Antifungal Antibiotic (항곰팡이성 항생물질의 생산하는 균주의 분리 및 동정)

  • 신영준
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.5
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    • pp.832-836
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    • 2000
  • A bactirium prohibiting the growth of fungus Botrytis cineria KT 433 was isolate and identified from soil. The isolated strain was gram positive, aerobic bacteria with cream color, round and mucoid. It showed ord form of 0.45$\times$1.1 ${\mu}{\textrm}{m}$ at the cultivated for 24 hrs and the ellipsoided endospore wer observed after culting for 72 hrs. The optimum growth temperature and pH wer 35$^{\circ}C$ and pH 5.0~8.0, respectively. It could assimilate daxtrin, maltose, glucose, mannose, ribose and collobiose as a sole carbon source. The isolated was confirmed to be a Bacillus sp. strain from the findings. The antibiotic from the isolated strain was stable up to 121$^{\circ}C$. The strain, especially, showed specific activity for mold and yeast. However, there was not significant antibacterial activity.

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An Analysis of Stress-Strain Relationships of Unsaturated soils Based on Bishop Stress (Bishop 응력에 의거한 불포화토의 응력-변형률 관계의 분석)

  • Oh, Seboong;Lee, Jong-Pil;Kim, Tae-Kyung;Kwon, Ohkyun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.4C
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    • pp.175-182
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    • 2009
  • The stress-strain relationship of an unsaturated soil was analyzed by Bishop stress descriptions in this study. The failure criteria could be defined uniquely by the Bishop stress and were also independent of matric suctions. In the low level of matric suctions the failure criteria have a linear relationship and the estimated criterion fitted the measured accurately. Deformation moduli in the small strain level were linearly increased generally with respect to Bishop stress.

Undisturbed Sampler for Characterizing the Behaviour of Weathered Granite Residual Soils (화강풍화토의 거동 특성 규명을 위한 비교란 시료채취기 개발)

  • 정순용;이승래
    • Geotechnical Engineering
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    • v.13 no.6
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    • pp.13-24
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    • 1997
  • In Korea, granite is abundant and occupies around two-thirds of the country's ground. Bven though weathered granite residual soils are widely distributed, undisturbed sampling of this soil is extremely difficult because of the particultate structure. This difficulty has kept away the researchers from investigating !he deformational characteristics of weathered granite residual soil. Thus, a special undisturbed sampling device was developed and undisturbed samples were prepared for triaxial compression (TX), resonant column(RC), and torsional shear (75) tests. Local deformation transducer (LDT) was fabricated for internal strain measurements during TX tests. Both undisturbed samples and statically compacted samples of same density were tested by using TX with LDT, RC, and 75 test equipments. The behaviour of statically compacted specimens was almost the same as that of undisturbed samples in the strain ranges below 1 percent. The stiffness and strength decreased with increasing degree of weathering. In case of undisturbed specimens, strains at failure are widely varied from 2 percent to 11 percent, and planes of failure are irrelevant to the angle of internal friction due to the inhomogeneous nature.

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Characteristics of Pullout Behavior of Soil Improvement(SI) Anchor (지반개량(SI)앵커의 인발거동특성)

  • 임종철;홍석우;송무효;강낙안
    • Geotechnical Engineering
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    • v.14 no.6
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    • pp.139-151
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    • 1998
  • SI anchor means the soil improvement anchor. The ground for supporting anchor is improved by JSP, and as a result, SI anchor body has about 80cm in diameter. SI anchor shows high pullout resistance by the frictional force between anchor body and ground, and the bearing capacity of anchor body. Especially the frictional force increases very much with increasing diameter of anchor body improved by JBP. In this study, model and field tests are made to analyse the mechanism of pullout resistance of SI anchor. Through model tests for the SI anchor in air dried sandy ground, strain fields of ground around SI anchor surface are analysed by a photo analysis method using the latex membrane on the wall of soil tank. The results of field tests are analysed by the strains measured by 10 strain gages attached on the inner wall of specially designed PVC pipe embedded in anchor body, and the strains of anchor body are also measured in the model tests.

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