• The Korean Journal of Mycology
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• v.51 no.4
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• pp.373-381
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• 2023

This study aimed to isolate and characterize wild yeast strains from the vineyard soil in Korea. Twenty yeast strains were isolated from vineyard soil in Gimpo-si, Gyeonggi-do, Korea, where Vitis labrusca cv. Campbell Early is grown. Eighteen strains were previously recorded in Korea. The remaining two, Cyberlindnera mrakii VG-21-10C and Starmerella bacillaris GR9 were not previously recorded in Korea. The mycological characteristics of VG-21-10C and GR9 were investigated. Both were oval-shaped, convex, and smooth. However, differences were evident in colony color and carbon assimilation activities. Strain VG-21-10C is white-colored and assimilates glucose, glycerol, D-xylose, D-cellobiose, D-saccharose, and D-raffinose as carbon sources. Strain GR9 is cream-colored and assimilates glucose, D-saccharose, and D-raffinose as carbon sources.

• The Korean Journal of Mycology
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• v.52 no.2
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• pp.115-123
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• 2024

In 2022, a fungal isolate, designated KNUF-22-049, was obtained from a soil sample collected from a field in Sang-ju, Gyeongsangbuk-do, Korea. The strain exhibited cultural and morphological characteristics, including colony color and size and the shapes and sizes of conidiogenous cells and conidia, that were consistent with those of Apinisia keratinophila. A phylogenetic analysis using the sequences of the internal transcribed spacer regions and the large subunit of the nuclear ribosomal RNA gene confirmed that isolate KNUF-22-049 is most closely related to A. keratinophila CBS 947.73^T at a species level. Both the morphological observations and phylogenetic analysis indicated that KNUF-22-049 is indeed an A. keratinophila strain. This study represents the first documentation of Apinisia keratinophila in Korea.

• Journal of the Korean Society of Safety
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• v.17 no.2
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• pp.52-57
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• 2002

In this study. stress-strain relationships were investigated by performing the confined extension tests for seven types of geosynthetics such as geotextiles, composite geosynthetics and geogrids. A comparison was made between unconfined and confined moduli for each geosynthetic material to quantify the soil confinement effect on stress-strain properties. A comparison was also made between the increase of moduli at the same strain level with the types of the geosynthetics to demonstrate the different stress-strain responses. Based on the result of the extension tests, the higher the confining stress, the larger the secant modulus of geosynthetics. The secant modulus at 5% strain is twice as much as that of 10% strain, especially there is a noticeable increasing of secant modulus for the two nonwoven geotextiles.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 1999.10c
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• pp.594-598
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• 1999

To analyze the characteristics of unconfined compressive strength of reinforced soil bricks made of clayey and sandy mixed with cement, lime, staple fiber and their combinatioin , a series of unified comparessive tests was performed. The resutls are summarized as follows ; 1) Reinforcing effect of reinforced clayed soil and that of soil brick of sandy soil mixed with cement and staple fiber is 8 times greater than no reinforced sandy sol. Therefore, the reinforcing effect seems to be greater in sandy soil than in clayey soil . 2) Lime shows a negative reinforcing effect in clayed soil but a little reinforcing effect in sandy soil. 3) It is appeared that strain at failure of soil brick reinforced with staple fiber is greater than that of unreinforced brick regrardless of soil's type.

• Geomechanics and Engineering
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• v.37 no.5
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• pp.499-510
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• 2024

In northern China, abundant summer rainfall and a higher water table can weaken the soil due to salt heave, collapsibility, and increased moisture absorption, thus the chlorine saline soil (silty clay) needs to be stabilized prior to use in road embankments. To optimize chlorine saline soil stabilizing programs, unconfined compressive strength tests were conducted on soil treated with five different stabilizers before and after soaking, followed by field compaction test and unconfined compressive strength test on a trial road embankment. In situ testing were performed with the stabilized soils in an expressway embankment, and the results demonstrated that the stabilized soil with lime and SH agent (an organic stabilizer composed of modified polyvinyl alcohol and water) is suitable for road embankments. The appropriate addition ratio of stabilized soil is 10% lime and 0.9% SH agent. SH agent wrapped soil particles, filled soil pores, and generated a silk-like web to improve the moisture stability, strength, and stress-strain performance of stabilized soil.

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1530-1543
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• 2024

With an increase in the commercialization of bioplastics, the importance of screening for plastic-degrading strains and microbes has emerged. Conventional methods for screening such strains are time-consuming and labor-intensive. Therefore, we suggest a method for quickly and effectively screening plastic-degrading microbial strains through dual esterase assays for soil and isolated strains, using p-nitrophenyl alkanoates as substrates. To select microbe-abundant soil, the total amount of phospholipid fatty acids (PLFAs) included in each soil sample was analyzed, and esterase assays were performed for each soil sample to compare the esterase activity of each soil. In addition, by analyzing the correlation coefficients and sensitivity between the amount of PLFAs and the degree of esterase activity according to the substrate, it was confirmed that substrate pNP-C2 is the most useful index for soil containing several microbes having esterase activity. In addition, esterase assays of the isolated strains allowed us to select the most active strain as the degrading strain, and 16S rRNA results confirmed that it was Bacillus sp. N04 showed the highest degradation activity for polybutylene succinate (PBS) as measured in liquid culture for 7 days, with a degradation yield of 99%. Furthermore, Bacillus sp. N04 showed degradation activity against various bioplastics. We propose the dual application of p-nitrophenyl alkanoates as an efficient method to first select the appropriate soil and then to screen for plastic-degrading strains in it, and conclude that pNP-C2 in particular, is a useful indicator.

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1475-1483
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• 2024

Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11^T, SW19^T, and YR1^T, were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11^T, SW19^T, and YR1^T belonged to the genera Agromyces, Rathayibacter, and Nocardioides, respectively. Strain W11^T was closely affiliated with Agromyces cavernae SYSU K20354^T (98.1%), strain SW19^T showed the closest affiliation with Rathayibacter rubneri ZW T2_19^T (97.0%), and strain YR1^T was most closely related to Nocardioides marmorisolisilvae KIS18-7^T (98.0%). The genome sizes of strains W11^T, SW19^T, and YR1^T were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of W11^T, SW19^T, and YR1^T with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11^T were anteiso-C_15:0, iso-C_16:0, and anteiso-C_17:0. In strain SW19^T, they were summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1ω 9c), anteiso-C_17:0, and anteiso-C_15:0. Strain YR1^T exhibited C_18:1ω 9c, C_18:0 10-methyl, TBSA, and anteiso-C_15:0 as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11^T, SW19^T, and YR1^T represent novel species within the genera Agromyces, Rathayibacter, and Nocardioides, respectively. Accordingly, we propose the names Agromyces silvae sp. nov., with the type strain W11^T (=KCTC 49818^T =NBRC 115999^T), Rathayibacter soli sp. nov., with the type strain SW19^T (=KCTC 49860^T =NBRC 116108^T), and Nocardioides terrisoli sp. nov., with the type strain YR1^T (=KCTC 49863^T =NBRC 116165^T).

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.2
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• pp.46-51
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• 2004

In this paper, reinforcement of soil shear strength by bamboo(substitute materials simulating a root system) are evaluated by soil strength parameters(apparent cohesion(c) and internal friction angle(tan${\Phi}$)), using simple shear tester which clearly depicts shear deformation and controls soil suction. The results show that the internal friction angle does not change under various soil suction conditions but the apparent cohesion, which reach a peak in suction of 45cm$H_2O$ near critical capillary head, is effected by soil suction. And the reinforcement of soil strength by bamboo are expressed by apparent cohesion more than internal friction angle. In addition the increment of apparent cohesion by bamboo reached a peak in suction 45cm$H_2O$ too.

• Geomechanics and Engineering
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• v.5 no.1
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• pp.57-70
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• 2013

Constant rate of strain (CRS) consolidation tests were conducted for undisturbed Ariake clay samples from three boreholes in Saga Plain of Kyushu Island, Japan. The coefficients of consolidation ($c_{\nu}$) were interpreted from the CRS test results by small- and large-strain theory. Large-strain theory was found to interpret smaller $c_{\nu}$ values and less strain rate effect on $c_{\nu}$ than that by small-strain theory. Comparing the theoretical strain distributions within a soil specimen to those obtained by numerical simulation shows that the small-strain theory can be used only for the dimensionless parameter $c_{\nu}/\dot{\varepsilon}H_0^2{\geq}50$ (where $\dot{\varepsilon}$ is strain rate and $H_0$ is the specimen height), and the large-strain theory can be used for a larger range of strain rates. Applying the criterion to undisturbed Ariake clay with a $c_{\nu}$ value of about $1{\times}10^{-7}\;m^2/s$, it is suggested that the large-strain theory should be adopted for calculating the $c_{\nu}$ value when $\dot{\varepsilon}$ > 0.03%/min.

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.43-46
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• 1995

For using additives of foods, or cosmetics, a strain A80 producing blue pigment was isolated from soil. The strain A80 was identified as a strain of Azotobacter vinelandii based on morphological and physiological characteristics. The strain A80 was extracellulaly secreted the blue pigment on PYG agar plate, but not secreted it into PYG broth. And then, the strain A80 was extracellulaly secreted the blue pigment in PYG broth containing 2.0% chitin, while the strain A80 was not secreted the blue pigment in PYG broth containing 2.0% chitin and 1% NaCl simultaniously.