• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.11 no.2
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• pp.12-18
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• 2015

The energy pile, used for both structural foundations and heat exchangers, brings about heat exchange with the ground formation by circulating a working fluid for heating and cooling buildings. As heat exchange occurs in the energy pile, thermal stress and strain is generated in the pile body and surrounding ground formation. In order to investigate the thermo-mechanical behavior of an energy pile, a comprehensive experimental program was conducted, monitoring the thermal stress of a cast-in place energy pile equipped with five pairs of U-type heat exchanger pipes. The heating and cooling simulation both continued for 30 days. The thermal strain in the longitudinal direction of the energy pile was monitored for a 15 operation days and another 15 days monitoring followed, without the application of heat exchange. In addition, a finite element model was developed to simulate the thermo-mechanical behavior of the energy pile. A non-linear contact model was adopted to interpret the interaction at the pile-soil interface, and thermal-induced structure mechanics was considered to handle the thermo-mechanical coupled multi-field problem.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.289-295
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• 1982

Streptomyces bobili (YS-40) isolated from soil was tested that it had drug resistance against penicillin, cephalosporin series antibiotics and other antibiotics in the previous paper. The treatment of Streptomyces bobili, (YS-40) with ethidium bromide (EtBr), acriflavine and sodium dodecyl sulfate. (SDS) resulted in the elimination of R-plasmid from the host strain. Minimum growth inhibitory concentrations (MIC) of Hg, Ag, penicillin-G, ampicillin, chloramphenicol, oxytetracycline, streptomycin and kanamycin were found to be 15, 10, > 3, 000, > 100, > 1, 000, > 100, < 5 and < 5$\mu\textrm{g}$/$m\ell$ respectively. Among the curing agents, EtBr was proved to be the most powerful compound for the elimination of R-plasmid in the strain and the elimination rate with EtBr(10$\mu\textrm{g}$/$m\ell$) was about 98%. Optimal pH to. the elimination of R-plasmid was pH 7.0 and the R-plasmid in the cells incubated for 24 hrs was proved to be eliminated most effectively. Aerial mass color, soluble pigment formation and reverse side color were reported to be often the plasmid associated characteristics of the R-plasmid bearing bacteria. But these characteristics of the uncured and cured Streptomyces bobili, (YS-40) showed no changes in the most of the pigment formation media tested in this work.

• Journal of the Korean Geosynthetics Society
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• v.3 no.1
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• pp.3-15
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• 2004

Geogrid reinforced soil structures with segmental block facing have been increased since 1990's, because of the convenience of installation and the flexible appearance. In this paper, the behavior of the segmental reinforced retaining wall was analysed with the results of field monitoring. The height and length of reinforced wall are 12m and 25m, respectively. The field measurement equipments are horizontal and vertical earth pressure cells, settlement plate, strain gauge, inclinometer, and displacement pin. Based on the field monitoring, the horizontal earth pressure was approximately 0.3times higher than that of the theoretical method and the maximum tensile strength of reinforcement was 26.2kN/m. The displacement of facing wall was 23mm at the point of 7.1m height of the wall and toward the wall facing. The results of the study indicate that the segmental reinforced retaining wall is in a stable condition because of good compaction & reinforcement effects, and long period of construction time. Finally, the computer program of SRWall is very useful tool to design the segmental reinforced retaining wall.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.207-214
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• 2012

Laccase- and peroxidase-free tyrosinase has commercial importance in the production of L-3, 4-dihydroxyphenylalanine (L-DOPA), which is mainly used in the treatment of Parkinson's disease. In the present study, isolation of an actinomycetes microbial strain capable of producing only tyrosinase is reported. Among all soil isolates, three individual colonies revealed black color around the colony in the presence of tyrosine. Further screening for laccase and peroxidase activities using syringaldazine denoted that one of the isolates, designated as RSP-T1, is laccase and peroxidase negative and produces only tyrosinase. The microbe was authenticated as Streptomyces antibioticus based on 16S ribotyping. Effective growth of this isolate was noticed with the use of medium (pH 5.5) containing casein acid hydrolysate (10.0 g/l), $K_2HPO_4$ (5.0 g/l), $MgSO_4$ (0.25 g/l), L-tyrosine (1.0 g/l), and agar (15 g/l). The scanning electron micrograph depicted that the microbe is highly branched and filamentous in nature. The enzyme production was positively regulated in the presence of copper sulfate. The impact of different fermentation parameters on tyrosinase production depicted that the maximized enzyme titer values were observed when this isolate was grown at 6.5 pH and at $30^{\circ}C$ temperature under agitated conditions (220 rpm). Among all the studied physiological parameters, agitation played a significant role on tyrosinase production. Upon optimization of the parameters, the yield of tyrosinase was improved more than 100% compared with the initial yield.

• Applied Biological Chemistry
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• v.38 no.6
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• pp.502-506
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• 1995

A Bacillus strain which produces ${\beta}-galactosidase$ with high transgalactosylation activity, was isolated from soil and tentatively designated as Bacillus sp. A1. When ${\beta}-galactosidase$ from Bacillus sp. A1 reacted with 40% (w/w) lactose, transgalactosylation ratio reached up to 90% at the 70% conversion of the initial lactose. The biosynthesis of the enzyme in Bacillus sp. A1 required lactose as an inducer and was repressed by glucose. Observing that the addition of amino acids to culture medium resulted in enhancing, to a significant extent, both the growth and the enzyme production of the strain, yeast extract and commercially available hydrolysates of protein were examined for the suitability as amino acid source. As it turned out, SMP, an enzymatic hydrolysis product of soybean protein from Fuji Oil Co.(Japan), was the most suitable for optimization of the culture medium. When Bacillus sp. A1 was cultured in the presence of 0.5% SMP and 2% lactose, the enzyme activity increased up to $1.8\;U/m{\ell}-broth$.

• Applied Biological Chemistry
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• v.50 no.1
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• pp.12-17
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• 2007

A biosurfactant-producing strain, Bacillus subtilis TBM 3101 was isolated from the soil sample at Tae-Baek Mountain through an antifungal test and emulsification assessment. The strain was assessed, regarding to the microbial growth, by physical and chemical test, surface tension, emulsification activity and stability. The surface tension of the isolate sharply decreased to the minimum 29mN/m at 48 h growth. Of note, its emulsification was stabilized to the highest degree when tributyrin was utilized as a substrate, indicating that in comparison to a variety of synthetic surfactants, the biosurfactant produced by the isolate was significantly similar to synthetic surfactant, tween 20. In addition, the biosurfactant showed high emulsification activity when soybean oil, crude oil and tetradecane were used as a substrate. Thus, these studies could contribute to the detection and development of biosurfactant beneficial to the environment and humans.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.333-340
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• 2014

The aim of this study was to isolate a potential multifunctional biocontrol agent from bacteria for control of multiple plant diseases as an alternative to fungicides. A total of 201 strains were isolated from soil undamaged by repeated cultivation in Sunchang and their ability to produce antibiotics, siderophores and extracellular enzymes such as protease, cellulase and amylase was investigated. Selected strain SCS3 produced cellulose, protease and amylase. This strain also produced siderophores and showed excellent antifungal activity against various phytopathogens. SCS3 was identified as Bacillus subtilis using 16S rRNA sequencing, and named Bacillus subtilis SCS3. Finally, physiological and biochemical characteristics of B. subtilis SCS3 were examined. From the results, B. subtilis SCS3 was found to be a useful multifunctional biocontrol agent against various phytopathogens.

• Korean Journal of Food Science and Technology
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• v.19 no.4
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• pp.324-330
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• 1987

A large number of methanol-assimilating yeasts and bacteria were isolated from samples of soil, sewage, decomposed milk and spoiled sweet-radish pickles. Among the yeasts, one strain was selected and identified as a strain of Candida boidinii. In 1% (v/v) methanol Candida boidinii YF-3 grew well and could grow in as much as 5%. This yeast required boitin for grwoth. Maximum growth was observed at $30^{\circ}C$ and pH 6 in a semisynthetic medium. The productivity was 2.72g dry cells per liter in batch culture with 1%(v/v) methanol and the cell yield for methanol was $0.39\;gg^{-1}$. The specific growth rate was $0.11\;h^{-1}$ and the generation time was 6.4 hours. The protein content of the cell was 45.5% and total nucleic acid content was 5.9%. The amino acid profile was as good as FAO standard for food protein.

• Journal of the Korean Geotechnical Society
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• v.29 no.12
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• pp.95-104
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• 2013

Recently, application of CSG is increasing in various design construction projects. At the initial stage of cementation CSG materials show the same mechanical characteristics as soil, however, as the cementation process develops, CSG materials gradually reveal material characteristics of concrete. The hardened CSG manifests elastic behavior such as maximum strength at small strain range and rapid brittle failure. In this research, PVA fiber stiffeners were used in order to: (1) reduce such brittle behavioral characteristics; (2) improve the relatively weak tension performance of CSG materials. The binding strength between the bed materials and fiber prevents rapid brittle failure and increases tensional strength of fiber reinforced CSG materials.Test results show that fiber reinforcement alone could induce the stress-strain characteristics of CSG materials from brittle failure to ductile failure and also increase the residual strength.

• Structural Engineering and Mechanics
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• v.76 no.3
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• pp.279-291
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• 2020

The seismic design of conventional frame structures is meant to enhance plastic deformations at beam ends and prevent yielding in columns. To this end, columns are made stronger than beams. Yet yielding in columns cannot be avoided with the column-to-beam strength ratios (about 1.3) prescribed by seismic codes. Preventing plastic deformations in columns calls for ratios close to 4, which is not feasible for economic reasons. Furthermore, material properties and the rearrangement of geometric shapes inevitably make the distribution of damage among stories uneven. Damage in the i-th story can be characterized as the accumulated plastic strain energy (W_pi) normalized by the product of the story shear force (Q_yi) and drift (δ_yi) at yielding. Past studies showed that the distribution of the plastic strain energy dissipation demand, W_pi/ΣW_pj, can be evaluated from the deviation of Q_yi with respect to an "optimum value" that would make the ratio W_pi/(Q_yiδ_yi) -i.e. the damage- equal in all stories. This paper investigates how the soil type and ductility demand affect the optimum lateral strength distribution. New optimum lateral strength distributions are put forth and compared with others proposed in the literature.