• Korean Journal of Food Science and Technology
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• v.29 no.5
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• pp.1028-1032
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• 1997

The strain No. 33, which produces cellulose-degrading enzyme, was isolated from soil. Yellow halo was identified when the culture supernatant of the strain was loaded onto agar plate containing 2.0% CMC using paper disc method. From scanning electron microscopic observation, the morphology of the stain was rod-shaped. For identification, several biochemical characteristics were tested, and this strain was identified to Bacillus sp. So, we named this strain as Bacillus sp. No. 33. The maximal growth was observed when the stain was cultured in the medium containing 1.0% glucose, 3.0% yeast extract, 0.5% $KH_2PO_4$, 0.02% $MgSO_4{\cdot}7H_2O$, pH 7.0 at $30^{\circ}C$ for 39 hours with shaking. The maximal enzyme production was accomplished using the medium containing 4.0% CMC, 2.0% yeast extract, 0.5% $KH_2PO_4$, 0.04% $MgSO_4{\cdot}7H_2O$, pH 7.0 at $30^{\circ}C$ for 42 hours with shaking.

• Korean Journal of Microbiology
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• v.36 no.2
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• pp.119-124
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• 2000

Thirty three strains of bacteria were isolated from the wastewater and soil contaminated a chemical softener, nrganopolysiloxane. Of these, five strains which showed higher activities for removal this chemical were finally selected for further study. By five strains the 2,500 mgll chemical softener was removed 65.2-67.9% at $37^{\circ}C$ for 5 days by shaking. The pH optimum for growth of W3721, S3712, and S3723 strain were at around pH 7.0-7.5, and W2811, and W2823 strain were at pH 6.5-7.0, respectively. The temperature optimum for growth of W3712 strain was at $37^{\circ}C$ and the other four strains were at TEX>$30^{\circ}C$. The optimal pH and temperature for removal by W3712 strain was initial pH 7.0 and $37^{\circ}C$ respechvely. The W3712 strain was identified and named as Corynebacterium pseudodiphtheriticum W3712 based on its morphological and physiological characteristics.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.188-194
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• 2023

Microbacterium elymi KUDC0405^T was isolated from the rhizosphere of Elymus tsukushiensis from the Dokdo Islands. The KUDC0405^T strain was Gram-stain-positive, non-spore forming, non-motile, and facultatively anaerobic bacteria. Strain KUDC0405^T was a rod-shaped bacterium with size dimensions of 0.3-0.4 × 0.7-0.8 ㎛. Based on 16S rRNA gene sequences, KUDC0405^T was most closely related to Microbacterium bovistercoris NEAU-LLE^T (97.8%) and Microbacterium pseudoresistens CC-5209^T (97.6%). The dDDH (digital DNA-DNA hybridization) values between KUDC0405^T and M. bovistercoris NEAU-LLE^T and M. pseudoresistens CC-5209^T were below 17.3% and 17.5%, respectively. The ANI (average nucleotide identity) values among strains KUDC0405^T, M. bovistercoris NEAU-LLE^T, and M. pseudoresistens CC-5209^T were 86.6% and 80.7%, respectively. The AAI (average amino acid identity) values were 64.66% and 64.97%, respectively, between KUDC0405^T and its closest related type strains. The genome contained 3,596 CDCs, three rRNAs, 46 tRNAs, and three non-coding RNAs (ncRNAs). The genomic DNA GC content was 70.4%. The polar lipids included diphosphatydilglycerol, glycolipid, phosphatydilglycerol, and unknown phospholipid, and the major fatty acids were anteiso-C_17:0 and iso-C_16:0. Strain KUDC0405^T contained MK-12 as the major menaquinone. Based on genotypic, phylogenetic, and phenotypic properties, strain KUDC0405^T should be considered a novel species within the genus Microbacterium, for which we propose the name M. elymi sp. nov., and the type strain as KUDC0405^T (=KCTC 49411^T, =CGMCC1.18472^T).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.7
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• pp.4588-4594
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• 2014

In this study, small scaled (1/30) laboratory chamber tests of the pile foundation for a lightweight concrete pavement system were carried out to evaluate the safety of a pile foundation on sandy soil. The testing ground was simulated in the field and a standard pile-loading test was conducted. The test piles were divided into 3 types, Cases A, B and C, which is the location from the center of the slab by applying a vertical load. The interval between the piles was set to 8 cm. As a result of the pile foundation model test, the pavement settled when the vertical load was increased to 12kg from 1.5kg in sandy soil ground, particularly the maximum settlement of 0.04mm. Judging from the model chamber test, Case A showed compressive deformation, whereas Case B represented the compression and tensile forces with increasing vertical load. Case C showed an increase in tensile strain.

• Journal of the Korean GEO-environmental Society
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• v.16 no.2
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• pp.27-31
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• 2015

In urban area, many design projects related to geotechnical projects are controlled by serviceability rather than stability requirements. Accordingly, control of ground deformation has become more crucial and many researchers have studied soil stiffness. Recent experimental studies on the stress-strain response of Chicago glacial clays showed that the nonlinearity and anisotropy are the two key factors in evaluating the soil stiffness. In this study, experimental results are applied to analyze the deep excavation site locating in downtown Chicago. The stress paths observed from the observation points located behind and front of the supporting wall yield typical stress paths. Changes in soil stiffness nonlinearity and anisotropy were discussed by comparing experimental and computed stress paths. The stiffness anisotropy were significant even at the first few excavations. The stiffness degradation characteristics are significantly different according to relative location to the support wall even at the same elevation.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.602-609
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• 2012

Burkholderia anthina R-4183, Burkholderia diffusa R-15930 and Burkholderia stabilis LMG 14294 isolated from green house soils (Gongju-Gun area, South Korea) were characterized and their phosphate solubilizing ability was assessed. Under in vitro culture conditions, all three species were proved to be effective in solubilizing phosphates in varying degrees. Strain Burkholderia anthina exhibited the highest phosphate solubilization in NBRIP medium ($665{\mu}g\;ml^{-1}$) followed by Burkholderia diffusa ($630{\mu}g\;ml^{-1}$) and Burkholderia stabilis ($578{\mu}g\;ml^{-1}$). However, solubilization of $FePO_4$ and $AlPO_4$ was found to be poor in all the strains. Acidification by means of gluconic and oxalic acids accumulation in the culture medium could be the possible mechanism responsible for phosphate solubilization. Glucose at the rate of 3% was found be the best carbon source for Burkholderia anthina while other two Burkholderia species showed maximum phosphate solubilization at 2% of glucose. In the case of nitrogen sources, ammonium and nitrate were equally effective in solubilizing phosphates by Burkholderia species. Despite a slight decrease in phosphate solubilization observed at increasing temperature, all three Burkholderia species could withstand a temperature of $30-35^{\circ}C$, pH at the range of 7-9 and the presence of NaCl (up to 2.5%) without much compromising the phosphate solubilization. As shown with potted mung bean seedlings, all the three isolates could enhance soil fertility and plant growth indicating their great potential to be used as bio-inoculants.

• Journal of the Korean Geosynthetics Society
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• v.10 no.2
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• pp.21-28
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• 2011

Several theoretical soil nonlinear models to predict damping ratio, which is one of the typical dynamic properties of soils, it is impractical to predict damping ratio. The resonant column and torsional shear test(RC-TS) is used to represent the dynamic behavior of soils from intermediate to medium shear strains. A limitation of RC-TS is measure precise shear strain in large strains and the modified equivalent radius($R_{eq}$) was obtained using both modified hyperbolic model and Ramberg-Osgood model. Bonneville clays were tested using RC-TS test to obtain rotation and torque. The measured rotation and torque were then compared with calculated rotation and torque using curve-fitting method. Then, the nonlinear soil model parameters were obtained and the equivalent radius was calculated using the model parameters.

• Journal of the Korean Geotechnical Society
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• v.19 no.6
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• pp.299-306
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• 2003

Dredged soil experiences large settlement during consolidation because of its high water contents. Large settlement alters the thickness of the consolidation layer greatly with time. However, the consolidation theory proposed by Terzaghi assumes the thickness of the consolidation layer to stay constant. Mikasa has developed a more rational theory considering the change of thickness of consolidation layer but it is not well applied at the site. In this study consolidation tests have been performed using Rowe cell for the four dredged clay samples with a water content of 100%, 120%, 133% and 150%. From the test results compression index characteristics and coefficient of consolidation characteristics have been investigated. Coefficients of consolidation obtained by Terzaghi's and Mikasa's theories, have been evaluated and compared with each other. When Mikasa theory is applied in the field design, the period to reach the required degree of consolidation has been reduced compared with the result by Terzaghi theory because the time factor $T_{v}$ by Mikasa theory decreases with increasing of final strain of consolidation layer, Calculation method consolidation time by Mikasa theory was concisely explained for its practical use.e.

• Proceedings of the KSR Conference
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• 2010.06a
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• pp.640-649
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• 2010

Coarse granular materials such as gravel and crushed stone have been used as an important fill materials to large soil structure of railway, road, dam and so on. Although much studies for general soil materials have been carried out domestically, the studies for coarse materials were insufficient. Particularly, it is the level in which the study for dynamic properties(Elastic modulus and damping ratio) of coarse materials, applies the foreign country literature. This is due to the lack of large equipment for element test. But large soil structures made of coarse granular materials are generally important infrastructures. Therefore, the reliable design parameters for coarse materials should be obtained for safe and economic design, construction and maintenance. Triaxial test is the laboratory test method that is capable of controlling a confining pressure and boundary condition. In this project, we made a multi-purpose large triaxial testing system. This testing system is able to test coarse granular materials with maximum particle diameter of 100mm and support both the load control and displacement control. The load cell is installed inside of triaxial cell and the axial displacement is measured locally in order to control and measure more accurately in the small strain level. The verification test of this testing system was carried out with urethane verification specimens. So, from now on the useful information for coarse granular materials are expected to suggested by performing many tests with various material and condition.

• Korean Journal of Food Science and Technology
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• v.45 no.6
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• pp.785-790
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• 2013

The purpose of this study was to investigate the hazards from onions and their cultivation areas. A total of 32 samples were collected from onion farms and tested for biological (sanitary indicators, and pathogenic bacteria and fungi) and chemical (heavy metals and pesticide residues) hazards. Aerobic bacteria and coliforms were detected at a level of 0.2-7.1 log CFU/g (or mL) in the soil and agricultural water, 1.6-3.6 log CFU/g on surface of the onion, 0.0-6.0 log CFU/hand (or $cm^2$) on the workers' hands, clothes, and gloves, and 4.7 log $CFU/cm^2$ on the onion bags. Fungi were detected at a level of 0.0-5.0 log CFU/g (or mL, hand, or 100 $cm^2$) in all the samples. Staphylococcus aureus was detected at a level of 1.2 log CFU/hand on the workers' hands, the detection level of Bacillus cereus was up to 4.8 log CFU/g in the soil. However, Escherichia coli (and in particular strain O157:H7), Listeria monocytogenes, and Salmonella spp. were not detected. Although heavy metals were detected in the environment (in soil and agricultural water) and pesticide residues were detected in onion, the levels were lower than the regulation limits.