• Title/Summary/Keyword: soil medium

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EPS Production, PHB Accumulation and Abiotic Stress Endurance of Plant Growth Promoting Methylobacterium Strains Grown in a High Carbon Concentration

  • Woo, Sung-Man;Subramanian, Parthiban;Ramasamy, Krishnamoorthy;Joe, M. Melvin;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.572-581
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    • 2012
  • In this study, we compared growth pattern, floc yield, Exo-polysaccharides (EPS) production, Poly-${\beta}$-hydroxybutyrate (PHB) accumulation, resistance to osmotic and acid stress in Methylobacterium strains CBMB20, CBMB27, CBMB35, and CBMB110. Modified high C:N ratio medium denoted as HCN-AMS medium was used with a C:N ratio of 30:1. The HCN-AMS medium favored increased growth in all the studied strains. All Methylobacterium strains tested positive for EPS production and showed positive fluorescence with calcoflour stain. Elevated levels of EPS production from 4.2 to 75.0% was observed in HCN-AMS medium. Accumulation of PHB in HCN-AMS medium increased by 3.8, 36.7, and 12.0% in strains CBMB27, CBMB35, and CBMB110 respectively. Among the abiotic stresses, osmotic stress-induced growth inhibition of Methylobacterium strains was found to be lowered when grown in HCN-AMS medium. Likewise, growth inhibition due to acid stress at pH 5.0 was lower for strains grown in HCN-AMS medium compared to growth in AMS medium. Enhanced survivability under stress conditions may be attributed to the high EPS and PHB production at increased carbon concentration in the growth medium.

Phylogenetic Characteristics of viable but Nonculturable Bacterial Populations in a Pine Mushroom (Tricholoma matsutake) Forest Soil (송이 자생군락 토양 내 난배양성 세균군집의 계통학적 특성)

  • Kim, Yun-Ji;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.43 no.3
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    • pp.201-209
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    • 2007
  • The CFDA (6-carboxyfluorescein diacetate) direct viable count method and plate count (PC) method using conventional nutrient broth (NB) medium and $10^{-2}$ diluted NB (DNB) medium were applied to samples collected from Mt. Yongdoo In Andong, in an effect to determine the number of living bacteria pine mushroom forest soil. The number of living bacteria determined via plate count in NB medium comprised $5{\sim}8%$ of the CFDA direct viable count, and the bacteria in the DNB medium comprised $40{\sim}47%$. This result indicated that viable but nonculturable (VBNC) bacteria existed in the pine mushroom forest soil at a high percentage. The phylogenetic characteristics of the VBNC bacterial populations in the samples of pine mushroom (Tricholoma matsutake) forest soil were analyzed via the direct extraction of DNA and 16S rDNA-ARDRA. The 115 clones from pine mushroom forest soil were clustered into 31 different RFLP phylotypes by ARDRA. Based on the 16S rDNA sequences, the 31 ARDRA clusters were classified into 6 phylogenetic groups: ${\alpha}-,\;{\beta}-,\;{\gamma}-Proteobacteria$, Acidobacteria, Actinobacteria and Firmicutes. Among these bacterial populations, approximately 85% were classified as members of phylum Acidobacteria. The Acidobacteria phylum was shown to exist abundantly in the pine mushroom forest soil.

Characteristics of Bacillus sphaericus PSB-13 as Phosphate Solublizing Bacterium Isolated from Citrus Orchard Soil (감귤원 토양에서 분리한 인산염 가용화 미생물 Bacillus sphaericus PSB-13의 특성)

  • Joa, Jae-Ho;Lim, Han-Cheol;Han, Seung-Gap;Chun, Seung-Joung;Suh, Jang-Sun
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.5
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    • pp.405-411
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    • 2007
  • This study was carried out to measure insoluble phosphorus fractions content fixed in different soil type and isolate a superior phosphate solublizing bacteria(PSB) producing free phosphate in citrus orchard soil. Distribution of insoluble phosphate fraction ordered Al-P>Ca-P>Fe-P in the investigated citrus orchards. Insoluble phosphate fraction such as Al-P, Ca-P, Fe-P were higher in volcanic ash than in non-volcanic ash soil. A PSB with high holo zone in PDA-P medium isolated from citrus orchard soil. This strain identificated by MIDI system as Bacillus sphaericus. The optimum growth of pH and temperature were at 4~5, $30^{\circ}C$, respectively. When Bacillus sphaericus cultured at $25^{\circ}C$, 150 rpm condition in LB broth medium included different phosphate. Bacillus sphaericus produced free phosphate in the culture broth medium from tricalcium-phosphate(207.0 ppm), aluminium phosphate(324.5 ppm) and hydroxyapatite(334.8 ppm) and Phosphatase activity of Bacillus sphaericus was higher at $35^{\circ}C$ culture condition than that of $25^{\circ}C$. Two type preparation inoculated Bacillus sphaericus made with carrier materials such as Bentonite, $CaCO_3$, Sodium alginate. Density of PSB in this preparation conserved at $10^5c.f.u.\;g^{-1}$ level during storage in different temperature condition for 7 month. It also showed that free phosphate produced at PDA-P medium.

Application of the Trickling Rice Straw Filter Medium for Livestock Wastewater Enhanced Soil Chemical Properties and the Growth of Orchard Grass (Dactylis glomerata L.) (축산폐수(畜産廢水) 살수여상충전(撒水濾床充塡) 볏짚 시용이 토양화학성과 오처드그래스(Dactylis glomerata L.)의 생육에 미치는 영향(影響))

  • Kim, Jeong-Je;Yang, Jae-E;Shin, Young-Oh;Shin, Myung-Kyo
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.4
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    • pp.334-340
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    • 1997
  • It was proven that rice straw can serve can serve as a good support medium for trickling filter with livestock wastewater treatment. The efficiency of removal of removal of environmentally undesirable components from the wastewater by the straw medium was no less inferior to other commonly used support materials. The treated straw was also proven to be a source of abundant plant nutrients. The growth of orchard grass was enhanced greatly by the application of large quantity of the support medium. A maximum yield of dry matter was obtained at the rate of 40 ton/ha. Inorganic NPK elements as background fertilizers were highly recommended for better development in an early stage of growth. The nutritional status of the soil was very much improved by the addition of the medium in terms of exchangeable cations(K, Ca, Mg and Na), available phosphate, and organic matter contents. Nutrient uptake by orchard grass such as N, P and cations was enhanced by rice straw medium treatment and that was proportional to the levels of treatment.

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Effect of Humified Straw Used as a Medium of Trickling Filter with Livestock Wastewater on the Growth of Bunching Lettuce (Lactuca sativa L., var. crispa) and Soil Chemical Properties (축산폐수(畜産廢水) 살수여상충전재(撒水濾床充塡材)인 볏짚의 부숙처리가 상추(Lactuca sativa L., var. crispa)의 생육(生育)과 토양화학성(土壤化學性)에 미치는 영향(影響))

  • Kim, Jeong-Je;Yang, Jae-E;Shin, Young-Oh
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.2
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    • pp.137-144
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    • 1996
  • A feasible way to utilize small-scale livestock farm wastewater was explored. In place of common support materials rice straw was employed as a support medium of trickling filter for pig farm wastewater treatment. The straw medium which was humified for 20 days after wastewater treatment and mixed with soil significantly enhanced in general the development of bunching lettuce grown under greenhouse conditions. Improvement of soil chemical properties in terms of fertility was also achieved by the addition of humified straw medium after wastewater treatment. It was found that rice straw served as a satisfactory support medium of trickling filters with the wastewater treatment concerning removals of biological oxygen demand, chemical oxygen demand, and suspended solids.

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In Vitro Mass Propagation and Soil Adjastment of Zanthoxylum piperitum var. inerme Makino through Apical Meristem Culture (生長點 培養에 依한 민초피나무(Zanthoxylum piperitum var. inerme Makino)의 器內 大量 增殖 및 土壤 活着)

  • Jeong, Woo-Gyu;Lee, Sang-Rae
    • Korean Journal of Plant Resources
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    • v.6 no.2
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    • pp.171-179
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    • 1993
  • This study was conducted to investigate the effect of growth regulators and medium composition on the growth of each stage in apical meristem culture for mass propagation of Zanthoxylum piperitum var. inerme Makino. The source material, shoot tip segments were taken from three-years old graft trees. Apical meristems were cultured in vitro on basal MS, GD, WS, half strength MS(1/2MS) and half strength GD(1/2GD) media supplemented with various concentrations for growth regulators(BA, IBA) and inorganic nutrients. The results summarized are as follows: 1. In culture establishment stage, ratio of culture establishment was 96.7% and the best resuit was obtained using MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 2. In shoot multitication stage, both shoot multiplication and growth were achieved in average 5.6cm. These results were obtained on in MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 3. In roothing stage, phloroglucinol(PG) acted as IBA synergist in root initiation. The most faverable combinations for root development was half-strength MS medium supplemented with 162mg/l PG and 0.2mg/l IBA, and ratio of rooting was 58.0%. 4. In Vitro formed plantlets were transplanted to paper pots in greenhouse with 85% of relative humidity. 96% of survival rate was obtained from artificial soil mix having same volume of sand, vermiculite, peat, and soil.

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Types and Physicochemical Characteristics of the Nursery Media Used in Major Strawberry-Farming Regions (주요 딸기주산지에서 이용되는 육묘배지 종류 및 이화학적 특성)

  • Park, Gab-Soon
    • Journal of Environmental Science International
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    • v.25 no.6
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    • pp.889-895
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    • 2016
  • In Danseong-myeon (myeon is a subdivision of), Sancheong-gun, and Sugok-myeon, Jinju City, pot seedling culture was used at the rates of 100% and 62%, respectively. Root restriction seedling culture was used relatively more frequently than pot seedling culture in Yangchon-myeon, Nonsan City, Guryong-myeon, Buyeo-gun, and Wolsan-myeon, Damyang-gun. Open-field seedling culture was used at the rates of 80% and 54% in Samnangjin-eup (eup is a subdivision of a gun), Miryang City and Ssangnim-myeon, Goryeong-gun, respectively. In Danseong, Sancheong, and Sugok, Jinju, granite soil was used as the medium for pot seedling culture at the rates of 90% and 80%, respectively. In Yangchon, Nonsan, Wolsan, Damyang, and Ssangnim, Goryeong, commercial bed soil and coir dust were used for seedling culture at the rates of 73%, 64%, and 60%, respectively. At the main production sites, the use of granite soil for seedling culture, highest rate in 2000-2005, continued to decrease; in 2011-2013, granite soil was used at the rate of 37%, while commercial bed soil and coir dust were used at the rates of 32% and 14%, respectively. The bulk density of commercial bed soil, expanded rice hulls, and coir dust was $0.11-0.16g/cm^{-3}$. Coir dust was the lightest material but had the highest and, 36.7% and 21.8%, respectively. In sandy loam soil and granite soil was low. The pH of all growth media was between 5.4 and 7.0, and the values were in the range of $0.15-0.66dS{\cdot}m^{-1}$. However it was necessary, to adjust the pH levels and enhance the of expanded rice hulls, sandy loam soil, and granite soil. Similarly, the fertilizer concentration and air permeability of coir dust need to be adjusted.

Screening of Organo Phosphorus Insecticide Fenitrothion-Degrading Microorganisms (유기인계 살충제 fenitrothion 분해미생물 탐색)

  • Choi, Hyuek;Kim, Bok-Jin;Bae, Do-Yong;Lee, Young-Deuk;Kang, Sun-Chul
    • Korean Journal of Environmental Agriculture
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    • v.17 no.3
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    • pp.279-285
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    • 1998
  • Fenitrothion-degrading microorganisms were isolated from 124 sampling sites of paddy, upland, forest and polluted soil, and wastewater. A total of 1,071 strains were isolated from each selective medium supplemented with 50mg/l of fenitrothion - nutrient agar (NA) 601, potato dextrose agar (PDA) 201, Actinomycetes isolation agar (AIA) 168 and basal salt medium (BSM) 101, respectively. Twenty-eight effective strains of them, which showed more than 80% degradation of fenitrothion by the gasliquid chromatography(GLC) analysis. were successfully selected from each liquid culture supplemented with 50mg/l of fenitrothion - NB 12(upland soil 3, paddy soil 3, forest soil 2, polluted soil 4), PDB 8(upland soil 1, paddy soil 2, forest soil 2, polluted soil 3) and PSB 8(upland soil 1, forest soil 1, polluted soil 6), respectively. Four strains - NPal, NFol, PFol and BPol, which have the most powerful degradation activity were finally selected among 28 fenitrothion-degrading microorganisms based on the degradation rate at the concentration of 100mg/l fenitrothion in enrichment media.

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Fertility Assessment of the Piggery Wastewater Trickling Filtrate for Orchard Grass (Dactylis glomerata L.) and Soil

  • Yang, Jae E.;Kim, Jeong-Je;Shin, Young-Oh;Shin, Myung-Kyo;Park, Yong-Ha
    • Journal of Applied Biological Chemistry
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    • v.42 no.1
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    • pp.34-39
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    • 1999
  • In search of a method to achieve sustainable agricultural practices, a trickling filter was employed for the piggery wastewater treatment, where rice straw was the support medium in place of more commonly used materials. The filtrate from the trickling filter were applied to a soil, on which orchard grass (Dactylic glomerata L.) was grown followed by amendment of the treated straw medium. Orchard grass was cut twice, and growth parameters and yields were measured. Soil chemical properties before and after harvesting orchard grass were analyzed. Development of the forage crop was greatly enhanced by the application of the filtrate in terms of fresh weight, dry weight, and the absorption of nutrients. Better growth and higher nutrient uptake were found in the second cutting of the orchard grass. Organic matter content, ionic intensity, exchangeable cations, and phosphate of the soil increased with the application of the filtrate. The relative high concentrations of salts in the piggery trickling filtrate, expressed in electrical conductivity and content of sodium, exerted no detrimental effect on the crop and soil.

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Site-response effects on RC buildings isolated by triple concave friction pendulum bearings

  • Ates, Sevket;Yurdakul, Muhammet
    • Computers and Concrete
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    • v.8 no.6
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    • pp.693-715
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    • 2011
  • The main object of this study is to evaluate the seismic response effects on a reinforced concrete building isolated by triple concave friction pendulum (TCFP) bearings. The site-response effects arise from the difference in the local soil conditions at the support points of the buildings. The local soil conditions are, therefore, considered as soft, medium and firm; separately. The results on the responses of the isolated building are compared with those of the non-isolated. The building model used in the time history analysis, which is a two-dimensional and eight-storey reinforced concrete building with and without the seismic isolation bearings and/or the local soil conditions, is composed of two-dimensional moment resisting frames for superstructure and of plane elements featuring plane-stress for substructure. The TCFP bearings for isolating the building are modelled as of a series arrangement of the three single concave friction pendulum (SCFP) bearings. In order to investigate the efficiency of both the seismic isolation bearings and the site-response effects on the buildings, the time history analyses are elaborately conducted. It is noted that the site-response effects are important for the isolated building constructed on soft, medium or firm type local foundation soil. The results of the analysis demonstrate that the site-response has significant effects on the response values of the structure-seismic isolation-foundation soil system.