• Journal of Ecology and Environment
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• 제46권3호
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• pp.218-242
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• 2022

Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA₃), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.

• 한국약용작물학회지
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• 제27권6호
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• pp.397-403
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• 2019

Background: Oplopanax elatus is widely distributed at high altitudes (about 1,100 m) in China, Russia and Korea. It is hard to propagate, breed, and difficult to grow. Hence, it has been designated as a rare and endangered medicinal plant. A study was conducted to establish a system for large scale seedling production of Oplopanax elatus in vitro and to find the ideal environment for its seedling growth. Methods and Results: In this study, the explants produced under in vitro conditions during our previous study were grouped into three categories (under 10 mm, 10 mm - 30 mm and above 30 mm) based on plant height and were transferred to the growth-chamber and greenhouse for two weeks in each setting for acclimatization. The plantlet category of above 30 mm showed good performance, and was further evaluated under three acclimatization methods as follows: three different growth media (commercial soil, commercial soil + perlite, commercial soil + sand), four shading levels (0%, 50%, 70%, 90%) and four altitude levels (157 m, 218 m, 601 m, 870 m) in Gangwon province of South Korea. As results, O. elatus seedlings showed better growth characteristics at 870 m of altitude, 70% shading level and in the commercial soil compared to other treatments. Conclusions: The regenerated seedlings of Oplopanax elatus obtained through plant tissue culture would be advantageous for use in large scale seedling production systems paired with a good acclimation method. For obtaining optimal results, it is recommended that seedling be acclimatized in a high altitude environment.

• 한국약용작물학회지
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• 제13권4호
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• pp.133-137
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• 2005

가시오갈피 체세포 배를 이용한 우량종묘의 대량생산체계를 확립할 목적으로 체세포 배의 생물반응기 배양을 통해 얻은 식물체의 순화에 관한 기초시험을 수행하였다. 가시오갈피 기내배양 식물체의 토양활착은 밀폐된 공간보다 비가림 하지 않은 차광막조건에서 양호하였고 순화에는 Klasmann 상토와 원예용상토가 좋았다. 배양실 10도와 차광막에서 3월20일 순화한 기내식물체의 순화 율은 모두 90% 이상으로 양호하였다. 배양실조건에서는 $26^{\circ}C$의 순화온도에서는 10% 이하의 극히 저조한 순화 생존율을 보였으나 포장 차광막조건에서는 최고 온도가 $30^{\circ}C$를 넘는 5월20일 순화 처리구에서도 68%의 높은 순화생존율을 보여 불리한 환경조건에서 차광막순화가 순화에 더욱 적합하였다. 차광조건으로는 50% 차광망 처리가 적합하였다. 차광막에서 순화한 식물체의 묘 소질은 3월10일 순화를 시작한 식물체가 가장 좋았다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.350-353
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• 2004

in order to develop a new technique for the removal of arsenic compoundsfrom acidified water, the removal of arsenic compounds by an acidophilic moss, Jungermannia vulcanicola Steph. was investigated in this study. The result of vial tests for arsenic removal is dependent on the biological activity of moss. The presence of phosphate inhibited the arsenic removal. And the acclimatization of moss by the media containing arsenic increased the its capability of arsenic remova.

• 한국산림과학회지
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• 제98권6호
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• pp.719-725
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• 2009

본 연구는 체세포배를 통해 증식된 서울오갈피(Eleutherococcus seoulensis)의 기외 대량 묘목생산을 위한 최적의 조건을 구명하기 위해 수행되었다. 어뢰형의 체세포배는 $GA_3$가 각각 3.0 mg/L와 5.0 mg/L가 첨가된 1/3 MS와 WPM 배지에 치상하여 4주간 발아시켰으며 발아된 유식물체는 0.2% 활성탄과 1.0 mg/L $GA_3$가 첨가된 1/2 SH 배지에 옮겨주어 신장을 유도하였다. 신장된 유식물체는 대기순화 효과를 관찰하기 위해 membrane filter가 부착된 대기순화 용기에 치상하였다. 대기순화 처리와 더불어 토양의 종류가 야외순화에 미치는 영향을 살펴보기 위해 혼합토(펄라이트+피트모스), 펄라이트, 서울 남산상토에 이식하였고, 서울 남산상토에서 대기순화 과정을 거친 식물체가 8주 후 70% 이상 생존하였다. 토양순화 과정을 거친 묘목은 야외 묘포장에 이식하였고, 그 결과 8주 후 95% 이상생존하였다.

• 한국약용작물학회지
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• 제19권1호
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• pp.9-15
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• 2011

This study was conducted to establish the optimal condition for conservation of genetic resources and micropropagation of Lilium cernum. Induction of bulbet of L. cernum was highly effective (9.2 bulb/explant) on 1/2 SH (Schenk and Hildebrandt) medium supplemented with 1.0 mg/L TDZ (Thidiazuron) and 0.1 mg/L NAA (Naphthaleneacetic acid). The treatment of 0.1 mg/L NAA increased root development (6.4 root/explant) under the in vitro condition. In addition, treatments of AC (Activated Charcoal) and ventilation were enhanced to develop number of shoots and to elongate length of leaf, bulb and root. Futhermore, the process of short-term soil acclimatization was promoted to strengthen the plantlets induced under the in vitro condition.

• 한국자원식물학회지
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• 제26권1호
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• pp.84-89
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• 2013

본 연구는 인삼 기내에서 생산된 재분화 식물체의 토양 순화를 위해 순화과정을 단축하고 생존율을 높일 수 있는 방법을 개발하기 위하여 실시하였다. $GA_3$를 $0.4mg\;l^{-1}$로 9시간을 처리하였을 때 토양에서 생존율은 59.6%로 가장 높게 나타났으며, 그 다음이 $1mg\;l^{-1}$에서 9시간을 처리하였 때 43.7%의 생존율을 나타냈다. 재분화 식물체의 뿌리길이가 4 cm 이상일 때는 48개체가 생존하여 생존율은 53.3%로 처리 중 가장 높게 나타났으며, 뿌리 무게는 4 g 이상일때는 46.7%가 생존하여 가장 높은 비율을 나타냈다. 4년생 때 지상부 생육 특성을 조사한 결과, 초장은 35.3 cm, 경장은 18.3 cm, 엽장은 12.1 cm, 엽폭은 4.8 cm로 이식 재배한 연풍의 생육 특성과 비교해 보면 재분화 식물체의 생육이 약간 떨어짐을 볼 수 있었다. 3년생 때 지하부 특성을 보면 근장은 15.3 cm, 동장은 4.5 cm, 동직경은 2.1 cm, 그리고 근중은 15.4 g으로 지하부도 지상부와 마찬가지로 대조구인 묘삼을 이식하여 재배한 처리구에 비하여 생육이 약간 떨어졌다.

• 한국산림과학회지
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• 제97권1호
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• pp.127-133
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• 2008

본 연구는 장뇌삼(Panax ginseng)의 효율적인 증식과 성공적인 토양 순화의 최적 조건을 구명하기 위해 수행되었다. 장뇌삼의 자엽을 시료로 사용하여 체세포배를 유도한 후, 유도된 체세포배를 발아시켜 얻은 자엽을 다시 시료로 사용하여 2차 체세포배를 유도함으로써 효율성을 증가시켰다. 2차 체세포배의 형성율은 MS 배지의 경우 약 53%이고, WPM 배지의 경우 약 48%인 것으로 나타나 1차 체세포 배의 형성율인 MS 배지와 WPM 배지의 각각 83%, 73%에 비해 약간 떨어지는 것으로 나타났다. 형성된 체세포배는 $GA_3$가 첨가된 WPM 배지에서 발아시키고, 무기염류의 양을 반감시키고, $GA_3$가 첨가된 WPM 배지에서 신장시킨 후, 무기염류를 줄인 SH 배지에서 대기 순화와 무당배양의 처리에 따른 줄기와 뿌리길이, 생존율 차이를 조사하였다. 기내에서 대기 순화와 무당배양 혼용처리 시 가장 정상적인 유식물체로 성장 시킬 수 있었고, 뿌리길이도 가장 길었으며, 생존율 또한 75%로써 가장 높은 생존율을 보였다.

• Journal of Plant Biotechnology
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• 제49권2호
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• pp.124-130
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• 2022

The application of traditional taro propagation methods for large-scale cultivation would be insufficient to meet the high demand for quality planting materials. Therefore, this study aimed to develop an in vitro micro-propagation technique for two local taro cultivars (cv.), Wangi and Putih. Taro cormels were collected from the Malaysian Agricultural Research and Development Institute (MARDI) germplasm (Serdang, Malaysia). Explants were taken from the shoot tip of cormels and initially cultured on Murashige and Skoog (MS) basal media for four weeks. The explants were then transferred to different multiplication media supplemented with different types and concentrations of cytokinins such as 6-benzylaminopurine (BAP ) and Thidiazuron (TDZ). Shoot production was quantified after six weeks of culture. The highest mean number of new shoots was produced by the Wangi cultivar on MS medium supplemented with 2.0 mg/l BAP (2.10 shoots), MS medium supplemented with 0.5 mg/l TDZ (2.18 shoots), and Gamborg B5 medium supplemented with 6.0 mg/l BAP (2.43 shoots). The maximum average number of the Putih cultivar shoots was obtained on MS supplemented with 2.0 mg/l BAP (3.57 shoots). MS basal media was used for root initiation, as it produced an average of 25 roots with an 11-cm length. Various types of substrate mixtures were used during acclimatization. The best acclimatization substrate for the Wangi cultivar was 100% peat soil, whereas the Putih cultivar grew optimally in a combination of peat and perlites at a 1:1 ratio. Taro plantlets require approximately 4 to 6 weeks to acclimatize before they can be transferred to the field.

• 한국자원식물학회지
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• 제36권6호
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• pp.588-596
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• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).