• Journal of Microbiology and Biotechnology
• /
• v.25 no.9
• /
• pp.1467-1475
• /
• 2015

The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA₁, GA₃, GA₇, GA₈, GA₉, GA₁₂, GA₁₉, GA₂₀, GA₂₄, and GA₅₃), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

• Asian Journal of Turfgrass Science
• /
• v.21 no.1
• /
• pp.39-49
• /
• 2007

Bispyribac-sodium is a new-type herbicide that prevents the occurrence of annual bluegrass by the suppression of anthesis and inflorescence emergence on the bent green. The greenhouse experiment was conducted to investigate interaction effect of the bispyribac-sodium with 21 soil- and foliar-applied herbicides in regards of herbicidal activity of annual bluegrass. The remarkable synergism was not found on the combination of bispyribac-sodium with benfluralin, pendimethalin, oryzalin, siduron, chlorphtalim, isoxaben, bifenox, tenylchlor, indanofan, bentazone, imazosulfuron, imazaquin, halosulfuron-methyl and limsulfuron. However, mixture of bispyribac-sodium with mecoprop, triclopyr, metsulfuron-methyl, cyclosulfamuron, pyrazosulfuron-ethyl and pyributicarb produced greater synergism of herbicidal activity when compared with unmixed, single application. Phytotoxicity was low on bentgrass green and fast recovery was observed. In future, it would be strongly necessary to do research to Investigate the effect of bispyribac-sodium combination with other herbicides under various environment and management practices on-site bentgrass green.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.22 no.1
• /
• pp.91-105
• /
• 2020

This study is the basic study for improving the range of influence and potency of mixing bars in the chamber of Shield TBM. Currently, there are many studies on disk cutters, cutter bits and segments in the study of the domestic Shield TBM. However, studies that mix soil and rocks that come from the membrane during the Shield TBM excavation and scatter them with screw conveyors are not as good as those abroad. In this study, the existing Shield TBM Chamber was manufactured as a miniature and the experiment. Inside the chamber, different sizes (4 mm, 6 mm, 8 mm, 10 mm) and colors (black, white, red, and blue) were used to form layers. This experiment was carried out by different shapes and sizes of RPM and mixing bars. In addition, the difference between a miniature model and a reclining one was checked to determine the effect of the direction of gravity on the mixing efficiency. This was done in the same way for all other conditions other than differences in the direction of gravity. Through this experiment, we identified the orientation of the chamber model, the size and shape of the mixing bar inside, and the mixing effect and torque depending on RPM. A comparative review of the mixing effect and torque confirmed that the shape and size of the mixing bar affect the mixing of samples, and that the direction of gravity affects torque.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.10
• /
• pp.1319-1326
• /
• 2014

Rapamycin, produced by the soil bacterium Streptomyces hygroscopicus, has the ability to suppress the immune system and is used as an antifungal, anti-inflammatory, antitumor, and immunosuppressive agent. In an attempt to increase the productivity of rapamycin, mutagenesis of wild-type Streptomyces hygroscopicus was performed using ultraviolet radiation, and the medium composition was optimized using glycerol (which is one of the cheapest starting substrates) by applying Plackett-Burman design and response surface methodology. Plackett-Burman design was used to analyze 14 medium constituents: M100 (maltodextrin), glycerol, soybean meal, soytone, yeast extract, $(NH_4)_2SO_4$, $\small{L}$-lysine, $KH_2PO_4$, $K_2HPO_4$, NaCl, $FeSO_4{cdot}7H_2O$, $CaCO_3$, 2-(N-morpholino) ethanesulfonic acid, and the initial pH level. Glycerol, soytone, yeast extract, and $CaCO_3$ were analyzed to evaluate their effect on rapamycin production. The individual and interaction effects of the four selected variables were determined by Box-Behnken design, suggesting $CaCO_3$, soytone, and yeast extract have negative effects, but glycerol was a positive factor to determine rapamycin productivity. Medium optimization using statistical design resulted in a 45% ($220.7{\pm}5.7mg/l$) increase in rapamycin production for the Streptomyces hygroscopicus mutant, compared with the unoptimized production medium ($151.9{\pm}22.6mg/l$), and nearly 588% compared with wild-type Streptomyces hygroscopicus ($37.5{\pm}2.8mg/l$). The change in pH showed that $CaCO_3$ is a critical and negative factor for rapamycin production.

• Journal of the Korean Society of Groundwater Environment
• /
• v.4 no.4
• /
• pp.199-211
• /
• 1997

As a part of study on geological disposal of radioactive waste, hydrogeochemical characteristics of deep granitic groundwater in Korea were investigated through the construction of a large geochemical dataset of natural water, the examination on the behaviour of dissolved constituents, and the consideration of phase stability based on thermodynamic approach. In granitic region, the contents of total dissolved solids increase progressively from surface waters to deep groundwaters, which indicates the presence of more concentrated waters at depth due to water-rock interaction. The chemical composition of groundwater evolves from initial $Ca^{2＋}$－(C $l^{－}$＋S $O_4$$^{2-}$) or $Ca^{2＋}$-HC $O_3$$^{－}$ type to final N $a^{＋}$-HC $O_3$$^{－}$ or N $a^{＋}$－(C $l^{－}$＋S $O_4$$^{2-}$) type, via $Ca^{2＋}$-HC $O_3$$^{－}$ type. Three main mechanisms seem to control the chemical composition of groundwater in the granitic region; 1) congruent dissolution of calcite at shallower depth, 2) calcite precipitation and incongruent dissolution of plagioclase at deeper depth, and 3) kaolinite-smectite or/and kaolinite-illite reaction at equilibrium at deeper depth. The behaviour of dissolved major cations (C $a^{2＋}$, $K^{＋}$, $Mg^{2＋}$, M $a^{＋}$) and silica is likely to be controlled by these reactions.

• Tunnel and Underground Space
• /
• v.9 no.3
• /
• pp.185-193
• /
• 1999

This paper presents results of dynamic analysis for a bridge in intersection part of two tunnels subjected to moving vehicle load. Since such a bridge system is very unusual due to the fact that it is located in tunnel, the dynamic characteristics of the structure can not be assumed as conventional one. The structure investigated in this study it a reinforced concrete bridge in the intersection part of Namsan Tunnel-1 and Tunnel-2 in Seoul. It is supported by temporary steel structure which shall be constructed during the period of replacing lining in Tunnel-2. Dynamic analysis was carried out for the system using a finite element model constructed by general purpose FE program SAP2000. For this purpose, the structure, lining of tunnels, and surrounding rock were represented by finite elements, while the rock region it truncated and on its outer boundary viscous dampers were placed to simulate radiation of elastic waves generated tunnels. Several types of vehicle with various driving velocities were considered in this analysis. The FE model including vehicle loadings was verified by comparing calculated peak particle velocity with the measured one. From the analysis, the impart factor for the bridge was estimated as 0.21, which indicates that the use of upper bound for the impact factor in design code is reasonable for this kind of bridge system.

• Journal of the Korean Geotechnical Society
• /
• v.27 no.9
• /
• pp.87-98
• /
• 2011

In this study, the magnitude and phase variation of dynamic earth pressure acting on a pile in liquefiable soils were analyzed using a series of 1g shaking table tests. In the case of a pile in dry sand, the value of the dynamic earth pressure was the highest near the surface due to the inertia force of the upper load on the pile and it decreased as the depth of the pile got lower. On the other hand, for a pile in liquefiable sand, the magnitude and shape of the dynamic earth pressure were similar to those of the excess pore pressure and was largely affected by the deformation of soils. Furthermore, the inertia force of the upper load and the dynamic earth pressure acted in opposite directions in cases of dry sand and saturated sand where low excess pore pressure had developed. However, after liquefaction, those force components near surface acted unfavorably in the same direction. Finally, the Westergaard’s solution was modified and proposed as a method to evaluate the magnitude of dynamic earth pressure acting on a pile during liquefaction.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.23 no.6
• /
• pp.723-730
• /
• 2012

The radar cross section(RCS) of a trihedral corner reflector(TCR) should be accurately computed when it is used as an external calibration target for a satellite synthetic aperture radar(SAR) calibration campaign. This paper presents the RCS analysis on a trihedral corner reflector which is installed on a calibration site, using the wave reflection from the rough surface and the wave diffraction from the TCR edges. The results in this paper show quantitatively the effect of the front surface on the RCS of a TCR. The difference of the RCS between a TCR in air and a TCR on a ground surface is computed by including the interaction term which consists of the edge diffraction from the TCR edges and the surface reflection from the front rough surface. The reflection coefficient of a randomly rough surface is a function of the surface roughness and dielectric constant of the surface. The RCS of $10{\lambda}$ size TCR on a ground is 0.46 dB higher than TCR in air at 9.65 GHz, and this can reach at maximum 1.55 dB depending on a surface condition and TCR size. The effect of the front surface on the RCS of a TCR increases, as the surface roughness decreases, the soil moisture increases, and the size of TCR in wavelength decreases.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.31 no.1B
• /
• pp.29-36
• /
• 2011

Evapotranspiration (ET) from the various surfaces needs to be understood because it is a crucial hydrological factor to grasp interaction between the land surface and the atmosphere. A traditional way of estimating it, which is calculating it empirically using lysimeter and pan evaporation observations, has a limitation that the measurements represent only point values. However, these measurements cannot describe ET because it is easily affected by outer circumstances. Thus, remote sensing technology was applied to estimate spatial distribution of ET. In this study, we estimated major components of energy balance method (i.e. net radiation flux, soil heat flux, sensible heat flux, and latent heat flux) and ET as a map using Mapping Evapo-Transpiration with Internalized Calibration (METRIC) satellite-based image processing model. This model was run using Landsat imagery of Gyeongan watershed in Korea on Feb 1, 2003 and Sep 13, 2006. Basic statistical analyses were also conducted. The estimated mean daily ETs had respectively 22% and 11% of errors with pan evaporation data acquired from the Suwon Weather Station. This result represented similar distribution compared with previous studies and confirmed that the METRIC algorithm had high reliability in the watershed. In addition, ET distribution of each land use type was separately examined. As a result, it was identified that vegetation density had dominant impacts on distribution of ET. Seasonally, ET in a growing season represented significantly higher than in a dormant season due to more active transpiration. The ET maps will be useful to analyze how ET behaves along with the circumstantial conditions; land cover classification, vegetation density, elevation, topography.

• Ecology and Resilient Infrastructure
• /
• v.3 no.2
• /
• pp.100-109
• /
• 2016

Low impact development (LID) facilities are established for the purpose of restoring the natural hydrologic cycle as well as the removal of pollutants from stormwater runoff. Improved efficiency of LID facilities can be obtained through the optimized interaction of their major components (i.e., plant, soil, filter media, microorganisms, etc.). Therefore, this study was performed to evaluate the performances of LID facilities in terms of runoff and pollutant reduction and also to provide an optimal maintenance method. The monitoring was conducted on four LID technologies (e.g., bioretention, small wetlands, rain garden and tree box filter). The optimal SA/CA (facility surface area / catchment area) ratio for runoff reduction greater than 40% is determined to be 1 - 5%. Since runoff reduction affects the pollutant removal efficiency in LID facilities, SA/CA ratio is derived as an important factor in designing LID facilities. The LID facilities that are found to be effective in reducing stormwater runoff are in the following order: rain garden > tree box filter > bioretention> small wetland. Meanwhile, in terms of removal of particulate matter (TSS), the effectiveness of the facilities are in the following order: rain garden > tree box filter > small wetland > bioretention; rain gardens > tree box filter > bioretention > small wetland were determined for the removal of organic matter (COD, TOC), nutrients (TN, TP) and heavy metals (Cu, Pb, Cd, Zn). These results can be used as an important material for the design of LID facilities in runoff volume and pollutant reduction.