• Molecules and Cells
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• 제23권3호
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• pp.370-378
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• 2007

A superoxide dismutase was purified 62-fold in seven steps to homogeneity from Methylobacillus sp. strain SK1, an obligate methanol-oxidizing bacterium, with a yield of 9.6%. The final specific activity was 4,831 units per milligram protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. The molecular weight of the native enzyme was estimated to be 44,000. Sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 23,100. The isoelectric point of the purified enzyme was found to be 4.4. Maximum activity of the enzyme was measured at pH 8. The enzyme was stable at pH range from 6 to 8 and at high temperature. The enzyme showed an absorption peak at 280 nm with a shoulder at 292 nm. Hydrogen peroxide and sodium azide, but not sodium cyanide, was found to inhibit the purified enzyme. The enzyme activity in cell-free extracts prepared from cells grown in manganese-rich medium, however, was not inhibited by hydrogen peroxide but inhibited by sodium azide. The activity in cell extracts from cells grown in iron-rich medium was found to be highly sensitive to hydrogen peroxide and sodium azide. One mol of native enzyme was found to contain 1.1 g-atom of iron and 0.7 g-atom of manganese. The N-terminal amino acid sequence of the purified enzyme was Ala-Tyr-Thr-Leu-Pro-Pro-Leu-Asn-Tyr-Ala-Tyr. The superoxide dismutase of Methylobacillus sp. strain SK1 was found to have antigenic sites identical to those of Methylobacillus glycogenes enzyme. The enzyme, however, shared no antigenic sites with Mycobacterium sp. strain JC1, Methylovorus sp. strain SS1, Methylobacterium sp. strain SY1, and Methylosinus trichosproium enzymes.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1977년도 춘계 학술대회지
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• pp.24.2-25
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• 1977

• Archives of Pharmacal Research
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• 제15권3호
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• pp.215-219
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• 1992

S. fradiae exhibited the highest acetanilide p-hydroxylation activity among the Streptomyces spp. screened. Studies with inhibitors (metyrapone, 2. 6-dichloroindophenol, $\alpha,\alpha'$-dipyridyl, o-phenanthroline) and an absorption peak after CO treatment suggested that S. fradiae hydroxylase activity was due to cytochrome p-450. This hydroxylase activity was increased to ten times in the cell extract containing 0.5 mM sodium azide. Furthermore, the sedimentary activity in $105,000\times{g}$ centrifugal forces and solubilization of the activity with Triton-X 100 implied that this enzyme was membrane bound monooxygenase. pH Optimum of the enzyme was 6.5 in membrane bound state.

• 한국균학회지
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• 제20권1호
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• pp.51-57
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• 1992

Mitochondria in Pleurotus ostreatus were isolated and purified by stepped sucrose density gradient centrifugation, to compare the effects of organic compound on the activities of mitochondrial ATPase in Basidiomycotina with those in mammalian cell. The effects of N, N'-dicycio-hexylcarbodiimide (DCCD), carbonyl cyanide m-chlorophenylhydrazone (CCCP), sodium azide and aurovertin known as compounds to be related to electron transfer system in mitochondria were studied. A activity of mitochondrial ATPase was inhibited by 64%, 57% and 53% in the presence of 0.25 mM DCCD, 0.02 mM sodium azide and 1.5 $({\mu}g/mg\;of\;protein)$ aurovertin B, respectively. It was stimulated by 22% in the presence of 0.15 ${\mu}M$ CCCP.

• 미생물학회지
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• 제28권4호
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• pp.297-303
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• 1990

Secretion of Serratia marcescens nuclease by E. coli harboring pNUC4 was investigated. 29.2, 54.2 and 16.6% of total nuclease were observed in culture medium, periplasm, and cytoplasm of E. coli, respectively. To investigate the secretion mechanism of Serratia nuclease by E. coli, secretion kinetics of nuclease was examined in the presences of sodium azide, and energy metabolism inhibitor; procaine, an exoprotein processing inhibitor; and chloramphenicol, a protein synthesis inhibitor. In the presence of sodium azide, periplasmic unclease was gradually decreased and the extracellular nyclease was linearly increased according to the incubation time. Similar results were obtained in presences of procaine and chloramphenicol. From these results, we concluded that two transport processes are involved in nuclease secretion: secretion of nuclease through the inner membrane is occurred by an energy-dependent process and probably requiring precusor processing: secretion of nuclease through outer membrane does not require energy, de novo protein synthesis, and precursor processing.

• Journal of Ginseng Research
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• 제18권3호
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• pp.175-181
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• 1994

We studied the folilar wiping effects of antioxidants (ascorbate, glutathione and sodium azide), which effectively inhibited the chlorophyll bleaching or completely recorved the early stage of photosynthesis of Panax ginseng C.A. Meyer, on photosynthesis, stomatal resistance, free sugar, starch, and total saponin contents of ginseng under the excess light intensity (45 kLux) during 6 days. Ascorbate and glutathione, endogenous antioxidant, recovered photosynehtsis and stomatal resistance, and reduced the photoinhibition by the excess light intensity (45 kLux) on free sugar, starch and total saponin contents. But sodium azide, exogenous $^{1}O_2$ quencher, showed negative effect. Therefore, we assumed that carbohydrates and saponin metabolisms of ginseng by antioxidants (ascorbate, glutathione) were normal. For the reduction of inhibition by excess light in ginseng a program for the higher activation of antioxidants and antioxidative enzymes in ginseng leaf will be desirable. Key words Antioxidants, ascorbate, glutathione, Photoinhibition, ginseng.

• 공업화학
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• 제30권1호
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• pp.81-87
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• 2019

에너지화 열가소성 탄성체 제조에 사용될 수 있는 새로운 계열의 glycidyl azide polymer (GAP)을 모색하기 위해 전구체인 poly(epichlorohydrin) (PECH)에 친핵체인 아지드기와 알콕시 및 알킬 아민을 도입하여 4가지 GAP copolymer polyol을 합성하고 물성에 대하여 고찰하였다. 이 GAP copolymer 합성반응은 이종의 친핵체를 1단계 반응으로 동시에 치환하여 미반응 sodium azide가 남지 않는 친환경적이며 효율적인 합성법으로 평가할 수 있다. 역개폐 짝풀림(inverse gated decoupling) $^{13}C$ NMR 분석법과 Fourier transform infrared (FT-IR) 분석법으로 상대적 치환율을 정량적으로 분석하였으며 반응 진행도를 모니터링 하였다. 합성된 GAP copolymer의 유리전이온도와 분자량은 differential scanning calorimetry (DSC)와 gel permeation chromatography (GPC)로 분석하였다. 합성된 poly($GA_{0.8}-butoxide_{0.2}$), poly($GA_{0.7}-n-butylamine_{0.3}$), poly($GA_{0.7}-dipropylamine_{0.3}$), poly($GA_{0.7}-morpholine_{0.3}$)의 유리전이온도는 $-39^{\circ}C$에서 $-26^{\circ}C$ 범위의 값을 나타내었다.

• 한국육종학회지
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• 제41권2호
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• pp.92-100
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• 2009

To investigate the mutagenecity of sodium azide (SA) and to select mutants having various agronomic characteristics in rice (Oryza sativa L. ssp. japonica), dry seeds of rice variety 'Suweon 472' were treated with 0, 0.5, 1.0, 2.0, and 4.0 mM SA solutions prepared in 0.1 M phosphate buffer (pH 3.0). Germination rate, seedling height and sterility were investigated in $M_1$ generation and chlorophyll mutations were observed in $M_3$ generation. Germination rate and seedling height decreased as the increase of SA concentration in $M_1$ generation, the maximum seed sterility (40.8%) was found at 4.0 mM SA concentration. Chlorophyll mutants were occurred in $M_3$ generation and the frequency calculated on a line basis was 13.5% at the same treatment. Many kinds of mutations for morphological and agronomic characters were observed and mutations with short culm and glabrous leaf were frequently found in $M_3$ generation. Interestingly, five mutant lines resistant to blast or bacterial blight (BB) were selected and evaluated with several isolates in $M_3$ generation although Suweon 472 has been known to be susceptible to blast and BB. These mutants showed all resistance to seven isolates of blast and a total of 76 lines among 2,567 lines evaluated showed resistant to race K1 of bacterial blight. Two mutant lines (440172 and 41272) showed different reaction to BB isolates from the other resistant mutants. A few kinds of endosperm mutants were also identified and most of them were waxy mutants.

• 식물조직배양학회지
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• 제27권2호
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• pp.89-94
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• 2000

큰용담의 기내배양에서 EMS를 처리한 경우 돌연변이체를 선발하기에 최적의 농도는 200 uM이며 농도가 높아질수록 생존률이 저하되었으며 줄기는 가늘고 나선형으로 꼬이고 백화현상이 가장 심하였다. Colchicine의 경우 30일 까지는 전 농도에서 84~97%의 높은 생존률을 보였으나 60일 이후에는 농도에 따라 생존률의 차이가 보였다 변이체가 단간이며 왜소하였다. MNU의 경우 10 $\mu$M에서 200$\mu$M까지는 89~97%의 높은 생존률을 보이다가 500$\mu$M이상에서는 18.2%로 급격한 감소를 보였으며 변이체의 잎의 형태가 도피침형에서 주걱형과 도란형으로 변형된 것과 잎의 청색에서 진보라색으로 변형된 2가지 type의 변이체가 형성되었다. Sodium azide를 처리한 경우 적정농도는 80$\mu$M였고 60일 배양 시 전반적으로 30~50%의 균일한 생존률을 보였고 초장이 아주 작고 다분얼성 변이체를 형성하였다. 형성된 shoot의 길이를 비교하여 보면 sodium azide, MNU의 경우에서는 약 1.0cm내외인 반면 colchicine의 경우 0.5 cm내외로 저조한 길이 생장을 보였다. RAPD에 의한 돌연변이체의 확인에서 농도가 가장 높은 500$\mu$M에서 변이 band가 관찰되었으며 돌연변이 계통에서 새로 형성된 band의 수는 MNU에서 2개 NaN3에서 1개로 전부 3개 (8.1%)의 변이 band가 나타났다.

• 약학회지
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• 제23권3_4호
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• pp.187-189
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• 1979

A new method for the synthesis of azido and thiocyanatoindoles by iodine and sodium azide or sodium thiocyanate in acetonitile at room temperature or 60.deg.C was found. Azidoindoles (3-azido-3-methyl -2-phenylindolenine, 3-azido-2,3-diphenylindolenine, 2-azidomethyl-3-metbylindole and 1-azido-1,2,3,4-tetrahyrocarbazole) and thiocyanatoindoles(3-thiocyanatoindole, 2-phenyl-3-thiocyanato indole, 3-methyl-2-phenyl-6-thiocyanatoindole and 2, 3-diphenyl-6-thiocyanatoindole) were prepared by this method in good yields.