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A New Soybean Cultivar, "Wonkwang" with Sprout, High Yielding, Disease and Lodging Resistance (내병.내도복 다수성 나물콩 신품종 "원광")

  • Oh, Young-Jin;Cho, Sang-Kyun;Kim, Kyong-Ho;Kim, Young-Jin;Kim, Tae-Soo;Kim, Jung-Gon;Yun, Hong-Tae;Moon, Jung-Kyung;Baek, In-Youl;Han, Won-Young;Kim, Hyun-Tae;Ko, Jong-Min;Kim, Yong-Duk;Kim, Dong-Kwan
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.158-162
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    • 2009
  • "Wonkwang" is a new sprout-soybean cultivar developed from the cross between Danyeobkong and MS91001 at the Honam Agricultural Research Institute (HARI) and National Institute of Crop Science (NICS), RDA, in 2007. The preliminary, advanced and regional yield trials to evaluate the performance of Iksan 56 were carried out from 2003 to 2007. This cultivar has a determinate growth habit, purple flower, grayish brown pubescence, yellow seed coat, Grayish brown hilum, lanceolate leaflet shape and small seed size (10.9 g/100 seeds). The maturity date of "Wonkwang" is three days later than the check variety, "Pungsan". It has a good seed quality for soybean-sprout and resistance to lodging. The soybean-sprouts grown from "Wonkwang" have high isoflavone ($3,481{\mu}g/g$)contents. This cultivar has resistance to soybean mosaic virus (SMV) and necrotic symptom (SMV-N) and other most troublesome soybean diseases which are bacterial pustule and black root rot. The grain yield of "Wonkwang"in the regional yield trials (RYT) for 3 consecutive years was averaged 3.05 ton per hectare, which was 8% higher than that of the check cultivar "Pungsan".

Effective Screening Methods for Lipoxygenase Isozymes in Soybean Seeds (콩 lipoxygenase 효소의 효율적인 검정법)

  • Kim, Young Jin;Park, Tae Il;Cho, Sang Kyun;Oh, Young Jin;Kim, Tae Soo;Kim, Jung Gon
    • Korean Journal of Breeding Science
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    • v.40 no.1
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    • pp.26-30
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    • 2008
  • Normal soybean seed contains three lipoxygenase isozymes called L-1, L-2, and L-3, respectively, which are responsible for the generation of undesirable grassy-beany flavors. Simple and effective methods for the detection of lipoxygenase isozymes were developed in soybean seeds. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been tried in separating these isozymes. It was done effectively on 7.5% separating gel and 4.5% stacking gel. However, no reliable method has been developed specifically for separating L-3, L-13 and L-23. Visual judging methods were based on the bleaching activities of lipoxygenase in contact with methylene blue and ${\beta}$-carotene. Sodium linoleate bleaching method was adopted to determine L-1 and L-2. Carotene bleaching and spectrophotometric methods were used to determine L-3. These systems were very rapid within one minute, furthermore only required a small piece of cotyledon (below 10 mg) and the other part could be used for generation advance after analysis. It was demonstrated that 200 seed samples could be analyzed per day by one laboratory assistant. The combination of visual judging methods and electrophoresis is suitable for breeding programs. It took 6.5 hours for analysis of 100 seed samples by one person.

Quantitative Variation of Total Seed Isoflavone and its Compositions in Korean Soybean Cultivars (Glycine max (L.) Merr.)

  • Kim, Hong-Sik;Kang, Beom-Kyu;Seo, Jeong-Hyun;Ha, Tae-Joung;Kim, Hyun-Tae;Shin, Sang-Ouk;Park, Chang-Hwan;Kwak, Do-Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.2
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    • pp.89-101
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    • 2019
  • The variation of content of 12 soybean seed isoflavone components was determined in the aglycone, glucoside, malonylglucoside and acetylglucoside groups of 44 Korean soybean cultivars grown in 2016 as well as in 2017. The total isoflavone content of the 44 cultivars averaged at $2935.4{\mu}g/g$ and was in the range of 950.6 to $5226.3{\mu}g/g$ for two years. Malonylglucoside group averaged at $2437.2{\mu}g/g$ with the highest proportion of isoflavone composition (83.0%). Significant differences were observed between cultivars, years and their interactions for both the total isoflavone and each composition group contents (P < 0.0001); however, no year-wise differences were observed for daidzein and genistin. The broad-sense heritability ($h^2$) within the set of 44 Korean soybean cultivars was as high as 0.93 for the total isoflavone content and was in the range of 0.8-0.92 for each composition group of isoflavone except for acetylglucoside. The total isoflavone content in cultivar group for soy-sprout was higher ($3850.4{\mu}g/g$) than that for the other cultivar groups of soy-paste and tofu ($3082.8{\mu}g/g$), black or green soybean cooked with rice ($2345.8{\mu}g/g$), and early maturity group ($1298.6{\mu}g/g$). The total isoflavone content of 'Sowonkong', a soybean cultivar for soy-sprout, was the highest ($5226.3{\mu}g/g$). In the cultivar group for soy-paste and tofu, the average isoflavone contents of 'Daepung', 'Daepung2ho', 'Saegeum', 'Uram', and 'Jinpung' were higher than $4000{\mu}g/g$. With the exception of small seeded cultivars with low isoflavone contents such as 'Sohwang' and 'Socheongja', the seed size and total isoflavone content were significantly negatively correlated in 2016 and 2017, respectively ($r=-0.47^{**}$ and $-0.49^{**}$). The number of days of growth from flowering to maturity did not affect the variations observed in isoflavone content.

Current Status and Perspectives of Quality Improvement in Sesame (참깨 품질 연구의 현황과 문제점 및 전망)

  • Lee, Bong-Ho;Lee, Jung-Il;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.s01
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    • pp.86-97
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    • 1988
  • Sesame(Sesamum indicum L.) is probably the most ancient oilseed crop known in the world. The seed of sesame is used in a variety of ways as food. The whole seed may be eaten raw, either roasted or parched, or fed to birds and stock. Sesame oil is used as a salad or cooking oil, in shortening, margarine and in the manufacture of soap. Minor uses are as a fixative in the perfume industry and formerly as a carrier for fatsoluble substances in pharmaceuticals such as penicillin. One of the minor constituents of sesame oil, sesamin, is used for its synergistic effect in pyrethrin insecticides, in addition of a small quantity of this substance markedly increases the effectiveness of fly sprays. The meal remaining after oil extraction can be used as and animal feed-stuff or as manure. In general sesame meal is considered to be equal to cottonseed or soybean meal as a protein supplement for livestock and poultry. It is especially high in certain amino-acids such as methionine, which is low in soybean meal, and thus can be combined with it or similar meal to form a more balanced ration. An attempt to summarize the literature review on quality improvement of sesame was made to discuss the accomplishments of the past and perspectives in the future. The reviews on quality improvement of sesame were mainly discussed in connection with the cultural practices and genetic informations in current status. The emphasis focussed on environmental variation of quality in cultural practices, such as harvest time, variety by location, climatic condition, fertilizer application, and growth regulator treatment. On the genetic variation of quality, it was discussed on variety background, mutation breeding, correlations, and inheritance of quality related characteristics. It also was discussed on relationship between quality and plant traits, storage condition or period, and seed coat color. Moreover, current research status were reviewed on some minor elements such as sesamin, oxalic acid, and trypsin inhibitor. As a results of the review, the lack of an effort to quality improvement in each utilization area was indicated as a problem area. More active efforts for the improvement of quality were also insufficient to incorporate the available genes for quality in breeding method or collection and analysis of breeding materials. Therefore, researches in the future would be recommended to emphasize on these problem areas.

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Comparison of morphometric traits between small yellow croaker (Larimichthys polyactis) and yellow croaker(L. crocea) (참조기(Larimichthys polyactis)와 부세(L. crocea) 간의 외부계측형질 비교)

  • Park, In-Seok;Oh, Ji Su
    • Korean Journal of Environmental Biology
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    • v.38 no.4
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    • pp.507-517
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    • 2020
  • Several methods including morphometric analysis were used to distinguish small yellow croaker (Larimichthys polyactis) from yellow croaker (Larimichthys crocea), which have very similar external shapes. Morphometric analysis showed four considerable differences (p<0.05) among the total 48 morphometric dimensions, but no differences were seen in the classical dimensions (p>0.05). Rather, significant differences were seen in two truss dimensions: Insertion of dorsal fin base - origin of pectoral fin base and origin of anal fin base - origin of pectoral fin base, and two head part dimension: most anterior extension of the head - above of eye and above of eye - posterior aspect of operculum(p<0.05). However, the yellow croaker had higher values than the small yellow croaker in the other three morphometric dimensions except for the head part dimension of above of eye - posterior aspect of operculum of the four morphometric dimensions (p<0.05). The X-ray photographs indicated that the small yellow croaker (45.1±2.34°) had 8.4% more curved vertebral column than the yellow croaker(38.4±1.82°). A diamond-shaped cranium was found when the skin was peeled off from both fish but the commonly held fact that only the small yellow croaker has a diamond-shaped cranium is not accurate. Our results confirmed that the two fish could be definitely distinguished by their external body shape.

Effect of Soil Temperature, Seedtime, and Fertilization Rate on the Secondary Growth in the Cultivation of the Big Bulbils of Namdo Garlic (Allium sativum L.) (남도마늘 대주아 재배시 지온, 파종시기 및 시비량이 이차생장에 미치는 영향)

  • Choi, Hak-Soon;Yang, Eun-Young;Chae, Won-Byoung;Kwack, Yong-Bum;Kim, Hong-Lim
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.454-459
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    • 2009
  • The secondary growth of garlic depreciates the quality of a bulb and, in the worse cases, makes it difficult to be used as a seed bulb due to many insertions of small cloves in the seed bulb. Therefore, the effect of soil temperature, seedtime, and fertilization rate on the secondary growth of the big bulbils of Namdo garlic has been examined. When we analyzed into the growth characteristics of big bulbils of Namdo garlic under different fertilizer application levels (50% increased fertilization, experimental fertilization and 50% decreased fertilization), the plant height, numbers of leaf, sheathe diameter, leaf length, and leaf width were the best in 50% increase of the test rate of fertilizer. The occurrence rates of secondary growth were 34.2% in 50% decrease of the test rate of fertilizer, 44.3% in the test rate, and 54.1% in 50% increase of the test rate of fertilizer. In other words, the occurrence rate of secondary growth increased by increasing the fertilization rate. While the average harvested bulb weight were 34.1g in 50% decrease of the test rate of fertilizer, 35.1g in the test rate of fertilizer, and 33.9g in 50% increase of the test rate of fertilizer, there were no changes in the number of cloves under different fertilization rates. As the correlation diagram between the soil temperature and occurrence of the secondary growth showed very high relation with 0.892~0.997, the secondary growth in cultivation of Namdo garlic big bulbils had considerably close correlation with the soil temperature. As the earlier the seedtime was, the growth of the above-groundparts including the plant height, numbers of leaf, and sheath diameter were the better. The averages of bulb weight were 36g in the mid September sowed seed bulb, 29.6g in the late September sowed seed bulb, and 27.9g in the early October sowed seed bulb. Overall, our results showed that the bulb size is dependent on the seedtime and the seedtime has no particular effects on the secondary growth.

Variation of Saponin Content in Korean Native Soybean Landraces Reintroduced from USA to Korea (미국으로부터 재도입된 한국 원산 콩 재래종의 Saponin 함량 변이)

  • Jang, Eun-Kyu;Piao, Xiangmin;Hwang, Tae-Young;Kim, Sun-Lim;Chung, Gyu-Hwa;Tsukamoto, Chigen;Choi, Yu-Mi;Lee, Myung-Chul;Lee, Jeong-Ran;Kim, Hong-Sig
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.286-295
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    • 2012
  • This study was carried out to assess the variation of saponin contents in 293 Korean native soybean landraces reintroduced from USA to Korea in 2007. Group A saponin content ranged from 149.8 ${\mu}g\;g^{-1}$ to 1279.0 ${\mu}g\;g^{-1}$ with an average of 484.9 ${\mu}g\;g^{-1}$. Group B saponin content ranged from 2160.1 ${\mu}g\;g^{-1}$ to 7868.6 ${\mu}g\;g^{-1}$ with an average of 3670.0 ${\mu}g\;g^{-1}$. Total saponin content including both group A and B contents ranged from 2502.8 to 8764.0 ${\mu}g\;g^{-1}$ with an average of 4154.9 ${\mu}g\;g^{-1}$. Five promising landraces for use as breeding materials that showed higher than any other landraces in group B and total saponin content were IT226841, IT226761, IT226841, IT226828 and IT228534. IT226841 showed the highest saponin content with group B content of 7868.5 ${\mu}g\;g^{-1}$ and total saponin content of 8764.0 ${\mu}g\;g^{-1}$ among landraces. In the relationship among each components, group A saponin content showed a positive correlation with group B saponin content (r=0.3708) and total saponin content (r=0.5119). Group B saponin content showed a very high positive correlation (r=0.9876) with total saponin content. When landraces were compared for the total saponin content based on collected location, landraces from North Korea showed the highest, Gyeonggi-do showed second highest content, followed by Chungcheongbuk-do, Gangwon-do, Gyongsangbuk-do, Chungcheongnam-do, Gyongsangnam-do, Jeollabuk-do and Jeollanam-do. Landraces were also grouped according to seed size and seed coat color. Small seed group was higher than medium and large seed groups that showed no significant difference in total saponin content. Seed coat colors showed no significant difference in total saponin content.

Selection and Quality Evaluation of Sprout Soybean [Glycine max (L.) Merrill] Variety for Environment-Friendly Cultivation in Southern Paddy Field (남부지역 친환경 논 재배를 위한 나물콩 품종 선발 및 품질 평가)

  • Kim, Young-Jin;Lee, Kwang-Won;Cho, Sang-Kyun;Oh, Young-Jin;Shin, Sang-Ouk;Paik, Chae-Hoon;Kim, Kyong-Ho;Kim, Tae-Soo;Kim, Ki-Jong
    • Korean Journal of Organic Agriculture
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    • v.19 no.3
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    • pp.357-372
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    • 2011
  • We carried out the experiment to select the suitable sprout soybean varieties for environment-friendly cultivation in paddy field of southern part area, compares of excess moisture injury degree and yield ability among 29 sprout soybean varieties. Plant growth of sprout soybean was generally low in beginning and recovered after flowering due to rainfall. In paddy field cultivation, number of pod per individual and number of seed per individual were less in difference than upland cultivation, and maturing date was delayed 5-14 days than upland cultivation in most species. When environment-friendly cultivation, pest injury was not caused major problem for the growth during the vegetative period of soybean due to ground spider as natural enemy to insect pest. However, damage of stink bugs showed severe during grain filling period, and Dawonkong, Anpyeongkong, Dachaekong and Wonhwangkong showed susceptible to sting bug. SMV infection was weak and showed some necrosis symptoms in Sokangkong, but black root rot was not infected at all. Bacterial pustule began to be infected slowly from pod enlargement stage in most species, displayed severe symptoms in Dawonkong, Pungsannamulkong, Seonamkong and Sobaeknamulkong. The symptoms of pod anthracnose, pod blight and purple spot were greatly appeared after flowering. Disease resistance varieties was Paldokong, Kwangankong, Doremikong, Somyeongkong, Pungsannamulkong, Iksa-namulkong, Seonamkong, Sojinkong, Pureunkong, Bosugkong, Namhaekong and Sorokkong. Lodging index showed 3 in Saebyeolkong, and other species displayed slight lodging in 0-3 degree. 100-seed weight is 9.8-17.2g extent and increased 0.1-3.7g than upland cultivation in most species, but decreased in some species. Government purchase standard, species correspond to small-seed-size namulkong (Sizing screen diameter 4.0-5.6 mm) was Dawonkong, Dachaekong, Bosugkong, Seonamkong, Sokangkong, Hannamkong, Somyeongkong and Wonhwangkong. Species which seed yield was higher than Pungsannamulkong (266kg/10a) were Sorokkong, Hannamkong, Bosugkong and Sowonkong. Considering sprout soybean species, disease endurance, insect resistance, lodging resistance, 100-seed weight, yield ability and excess moisture tolerances synthetically, Seonamkong, Hannamkong, Doremikong, Bosugkong, Pungwonkong, Kwangankong, Sowonkong, Dagikong, Paldokong, Eunhakong and Pungsannamulkong were promising for environment-friendly cultivation in paddy field.

Evaluation of External Quality of Brand Soybeans (콩 시판 브랜드 제품의 외관 품질 평가)

  • Jong, Seung-Keun;Woo, Shun-Hee;Kim, Hong-Sig
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.3
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    • pp.239-248
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    • 2007
  • Although high nutritional values and continuous identification of important functional substances of soybean [Glycine max (L.) Merrill.] promote consumption of soybean products worldwide, informations on quality of brand soybean is not enough for consumers. Total of 100 brand soybeans [32 for soypaste and source, 45 black testa (lage), and 17 black testa (small) or medicinal soybean and beansprout soybean] were collected at supermarkets and several external quality factors were analyzed. Brand soybeans were marked with the environmental friendly and intimating words along with soybean (white or yellow), black soybean (black-, frost-, late frost-, green or inner-green-), medicinal soybean and beansprout soybean. Among 100 brand soybeans 30% was 1 kg package and 59% was 500 g package, difference between printed and actual weights of 70% brand soybeans was ${\pm}1%$ and weights of 2/3 of brand soybeans were higher than printed weight. Range of 100 seed weights of soypaste and source, black testa (large) and black testa (small) and beansprout soybeans were $23.7{\sim}47.8g$, $21.9{\sim}44.5g$ and $9.5{\sim}15.0g$, respectively. Although ranges of 100 seed weights of soypaste and source and black testa (large) soybeans were similar, 63% of soypaste and source were less than 29 g, while 78% of black testa (large) soybeans were higher than 30 g. Although average and highest percentages of seeds separated with 6.7 mm sieve were similar with 87.4% and 99.9% for soypaste and source soybean and 86.5% and 99.5% for black testa (large) soybean, respectively, the lowest percentages were 70.7% for soypaste and source soybean and 14.4% for black testa (large) soybean. When 100 seed weight was greater than 35 g, 90% of seeds were remained on 6.7 mm sieve. On the other hand 100 g weight and percentage of seeds remained on 6.7 mm sieve showed significantly positive correlations [r=0.7488** for soypaste and source soybean and r=0.7874** for black testa (large) soybean when 100 seed weight was $20{\sim}30g$. Based on hilum color and/or appearance, 76% of brand soybeans collected (more than 90% in yellow testa soybeans) were found to be mixed more than 10% with other cultivars or landraces. Foreign materials such as sand, piece of clothe, wood piece, dead insects, other soybeans were found in 20% of brand soybeans. Average test weight of brand soybeans was 762g $L^{-1}$ with a range of $645{\sim}820g\;L^{-1}$. Soybeans from local markets were as good as brand soybeans in 100 seed weight, uniformity of seeds, weight of foreign materials and test weight.

Comparison of Salinity and Sodium Content by the Salinity Measurement Frequency of Soups of Childcare Centers Enrolled in the Center for Children's Food Service Management in Daegu (대구지역 어린이급식관리지원센터 등록 어린이급식소의 염도 측정 빈도에 따른 국의 염도 및 나트륨 함량 비교)

  • Lee, Na-Yeong;Lee, Yeon-Kyung
    • Korean Journal of Community Nutrition
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    • v.25 no.1
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    • pp.13-20
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    • 2020
  • Objectives: This study examined the salinity of soups provided at childcare centers by measuring the salinity for three years and providing basic data for sodium reduction. Methods: The soup salinity was measured using a Bluetooth salinity meter from January 2015 to December 2017 at 80 childcare foodservice establishments enrolled in the Suseong Center for Children's Foodservice Management in Daegu. Results: An analysis of the soup salinity each year showed that the salinity decreased significantly from 0.48% in 2015 to 0.41% in 2017, particularly in clear soups and soybean soups compared to other soups (P < 0.05). The salinity and sodium content in seafood soups (0.45% and 179.1 mg/100 g, respectively) were highest, followed by soybean soups (0.44%, 175.2 mg/100 g), with perilla seed soups containing the lowest (0.42%, 167.2 mg/100 g) (P < 0.05). The salinity was significantly higher in institutional foodservice establishments than small foodservice establishments (P < 0.001). The salinity and sodium content were the highest in foodservice establishments with a small number of measurements, and the salinity was the lowest in foodservice establishments with salinity measurements performed an average of 151 times each year (three times a week) or more (P < 0.05). The soup salinity was low in the order of winter, spring, summer, and autumn, and the salinity decreased significantly year by year in all seasons. (P < 0.05). Conclusions: The soup salinity was significantly lower in foodservice establishments where the salinity was measured more than three times a week, indicating that continuous salinity management is effective.