• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.491-498
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• 2007

It has been hypothesized that efficient exclusion of methylated retrotransposons and repeated DNA region is one of the rapid and cost-effective approaches for comprehensive gene discovery in large genome size of maize. Three kinds of methylation-sensitive restriction enzymes, HapII, MspI and McrBC, were used to identify the restriction frequency of cytosine methylation sites in maize genome. Roughly 60% of total maize genomic DNA was restricted less than 500bp by McrBC, and the most of restricted small size fraction was composed retrotransposon. In order to validate the efficient construction of gene-rich shotgun library, we compare two gene-rich methyl-filtration shotgun libraries using in vivo and in vitro methyl-filtration system. The size selected DNA fraction by Sau3A-McrBC enzyme treated was very stable and has not appeared modification in E. coli, but most insert DNA size of partially digested with Sau3A were decrease less than 500bp by bacterial methylation-modification system. In compare of retroelements portion, A 44.6% of the sequences were retroelement in unmethyl-filtered library, and the most of them was Copia type, such as Prem, Opie and Ji. The portion of retroelement was drastically decreased to 25% and 20% by in vivo and in vitro filtration system, respectively.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2005.11a
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• pp.189-192
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• 2005

The purpose of this study is to analyze space composition and size of the Master Zone in the apartment unit plan. For this, we analyzed 435 samples by searching web-sites of the 5 main construction companies. The major results of this study are as follows. 1) Master Zone is organized 4 spaces such as main room, bathroom and make-up & dress room. Six types of Master Zone are classified with the composition of 4 spaces. 2) The size of the Master Zone is increased in proportion to the square meter. But the range of the ratio is small range of fluctuation as 20%${\sim}$30%. 3)The size and ratio of the each spaces of the Master Zone are analyzed. The size of bedroom is increased but the ratio is decreased. The size of bathroom is increased but the ratio is regular as 4%${\sim}$5%. The size of make-up and dressing room is generally increased but the range of the size and ratio is the most extensive. Through this analysis and research, this study would be provied the basic data for actual housing planning and to contribute to future trends of apartment houses.

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.3
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• pp.148-154
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• 2021

We propose the AI Smart Factory Model for integrated management of production processes in this paper .It is an integrated platform system for the production of food packaging containers, consisting of a platform system for the main producer, one or more production partner platform systems, and one or more raw material partner platform systems while each subsystem of the three systems consists of an integrated storage server platform that can be expanded infinitely with flexible systems that can extend client PCs and main servers according to size and integrated management of overall raw materials and production-related information. The hardware collects production site information in real time by using various equipment such as PLCs, on-site PCs, barcode printers, and wireless APs at the production site. MES and e-SCM data are stored in the cloud database server to ensure security and high availability of data, and accumulated as big data. It was built based on the project focused on dissemination and diffusion of the smart factory construction, advancement, and easy maintenance system promoted by the Ministry of SMEs and Startups to enhance the competitiveness of small and medium-sized enterprises (SMEs) manufacturing sites while we plan to propose this model in the paper to state funding projects for SMEs.

• The Journal of Engineering Geology
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• v.25 no.2
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• pp.273-280
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• 2015

Delays in horizontal well drilling when encountering heterogeneous sediments can have negative effects on the construction process at a riverbank filtration site. Grain size analysis, including calculation of the coefficient of uniformity and the coefficient of curvature, was conducted on soil samples collected at each drilling depth during the process of drilling horizontal wells. These results were then used to develop a linear equation for estimating drilling velocity using the coefficient of uniformity and the coefficient of curvature as inputs. Testing of the linear equation in other horizontal wells indicates that the equation is most appropriate for coarse-sand-sized and well-sorted sediment. Because this study was conducted in a region with small- to medium-sized streams, more data are needed from larger rivers to modify the general equation. Our results will provide better estimates of drilling velocity, in turn enabling more detailed design and more effective construction management at riverbank filtration sites.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.673-676
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• 2008

Recent trends show an increased usage of 'colored concrete', a inorganic pigmented concrete mix, especially in small to large scale buildings. However, due to lack of regulations, current usage of colored concrete indicates a lack of consideration for safety factors and aesthetic aspects. Sometimes color pigmentation used in paints are inappropriately used in concrete, and in many cases the addition of coloring material is done without proper research into how structural characteristics of resulting concrete may have been affected. To resolve these issues, some construction sites apply ASTM or ACI regulations. However, such regulations incorporate elements that cannot be applied in Korea, which makes their domestic application impractical. In this paper, the primary aim is to determine to what extent the basic material characteristics of concrete is affected by the variety and quantity of different color pigments, and in so doing establish a foundation for future reference in case of construction projects involving the use of colored concrete.

• Journal of Aerospace System Engineering
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• v.14 no.5
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• pp.83-90
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• 2020

Disasters are causing significant damage to the lives and property of our society and are recognized as social problems that need to be solved nationally and globally. The 4th industrial revolution technologies affecting society as a whole such as the Internet of Things(IoT), Artificial Intelligence(AI), Drones(Unmanned Aerial Vehicles), and Big Data are continuously absorbed into the disaster and safety industries as scientific and technological tools for solving social problems. Very soon, twenty-nine domestic UAV-related organizations/companies will complete the construction of a multicopter type small UAV integrated system ('17~'20) that can be operated at disaster and security sites. The current work considers and proposes the mid-to-long term R&D direction of disaster UAV as a strategic asset of the national disaster response system. First, the trends of disaster and safety industry and policy are analyzed. Subsequently, the development status and future plans of small UAV, securing shortage technology, and strengthening competitiveness are analyzed. Finally, step-by-step R&D direction of disaster UAV in terms of development strategy, specialized mission, platform, communication, and control and operation is proposed.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.3
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• pp.33-48
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• 2009

A palustrine wetland is a type of a wetland that prevails in Korea as well as an ecosystem that provides various ecological functions and has affluent biodiversity. This study was conducted to understand the characteristics of vegetation in a palustrine wetland by analyzing a life form and naturalized plants, in order to present the maintenance and utilization plan of a small wetland. A total of 249 taxa including 76 families, 188 generics, 209 species, 38 varieties and 2 forma were found at six investigated wetlands. As a result of the appearance ratio analysis, Therophytes (37.8%) with 94 taxa and Hemicryptophytes (31.3%) with 78 taxa were in order of life form, and Gramineae (14.1 %) with 35 taxa and Compositae (11.2 %) with 28 taxa were in order of family level. These were deemed representative vegetation in a palustrine wetland. Equosetum arvense, Phragmites communis, Commelina communis, Persicaria thunbergii, Arenaria serpyllifolia, Stellaria media, Erigeron annuus, Erigeron canadensis and Artemisia princeps var, orientalis were found continuously for a period of investigation in all sites. Equosetum arvense, Phragmites communis and Persicaria thunbergii were species with high appearance frequency. Especially, Persicaria thunbergii was construed high in a cover ratio in May as it develop first among Hydrophytes and form a canopy sooner than anything else. We found that a degree of naturalization and an urbanization index appear comparatively high in Yeongok-ri, Cheonan, Gangjang-ri, Asan and Yodang-ri, Hwasung, which is construed due to artificial interference, such as fanning, fishing, swimming and green-tour program. These results would be used to utilize in restoring a deserted and damaged wetland and to provide fundamental data in creating a wetland as follows.

• Tribology and Lubricants
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• v.37 no.6
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• pp.253-260
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• 2021

Metal pipes are used in a wide range of applications, from plumbing systems of large construction sites to small devices such as medical tools. When a liquid is enforced to flow through a metal pipe, a higher flow rate is beneficial for higher efficiency. Using high pressures can enhance the flow rate yet can be harmful for medical applications. Thus, we consider an optimal geometrical design to increase the flow rate in medical devices. In this study, we focus on cannulas, which are widely used small metal pipes for surgical procedures, such as liposuction. We characterize the internal flow rate driven by a negative pressure and explore its dependence on the key design parameters. We quantitatively analyze the suction characteristics for each design variable by conducting computational fluid dynamics simulations. In addition, we build a suction performance measurement system which enables the translational motion of cannulas with pre-programmed velocity for experimental validation. The inner diameter, section geometry, and hole configuration are the design factors to be evaluated. The effect of the inner diameter dominates over that of section geometry and hole configuration. In addition, the circular tube shape provides the maximum flow rate among the elliptical geometries. Once the flow rate exceeds a critical value, the rate becomes independent of the number and width of the suction holes. Finally, we introduce a slippery liquid-infused nanoporous surface (SLIPS) coating using nanoparticles and hydrophobic lubricants that effectively improves the flow rate and antifouling property of cannulas without altering the geometrical design parameter.

• Genomics & Informatics
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• v.2 no.4
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• pp.174-179
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• 2004

We developed various plasmid cloning vectors that are useful in the construction of genomic and shotgun libraries. Two medium copy vectors, pCUGlblu21 (pCb21) and pAGlblu21 (pAb21), which are resistant to kanamycin ($Km^R$) and chloramphenicol ($Cam^R$), respectively, are useful for cloning DNA inserts ranging from 5kb to 15kb. Two high copy vectors, pCUGlblu31 (pCb31) and pAGlblu31 (pAb31), containing $Km^R$ and $Cam^R$, respectively, are useful for DNA inserts less than 5kb. These vectors are well adapted for large-scale genome sequencing projects by providing choice of copy number and selectable marker. The small vector size is another advantage of these vectors. All vectors contain lacZa including multicloning sites that originated from pBluscriptllsk- for easy cloning and sequencing. Two medium copy vectors contain unique and rare cutting Swal (ATTTAAAT) restriction enzyme sites for easy determination of insert size. We developed two combined vectors, pC21A31 and pC31A21, which are combinations of (pCb21 + pAb31) and (pCb31 + pAb21), respectively. These two vectors provide four choices of vectors such as $Km^R$ and medium, $Cam^R$ and high, $Cam^R$ and medium, and $Km^R$ and high copy vectors by restriction enzyme cutting, dephosphorylation, and gel purification. These vectors were successfully applied to high throughput shotgun sequencing of rice, tomato, and brassica BAC clones. With an example of extremely biased hydro sheared 3 kb shotgun library of a tomato BAC clone, which is originated from cytogenetically defined peri-centromeric region, we suggest the utility of an additional 10 kb library for sequence assembly of the difficult-to-assemble BAC clone.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.4
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• pp.119-132
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• 2012

The unmanaged abandoned concrete roads are vulnerable toward the issues on soil and water pollution, which requires flexible managing method such as eco-corridor after the process of ecological restoration. Among various alternations of abandoned concrete-paved roads, ecological restoration technique may be the most suitable method in sites including high quality of natural environment. Therefore, as in Young dong province, GyungBu express highway (227.24~229.04km), which is near to Hwang-gan IC, the survey to measure its effect of soil under the paving and water pollution by abandoned concrete roads was discussed. Then, the restoration method of plantings of landscape trees and hydro-seeding methods of artificial soil media was appraised through consecutive monitoring. The soil adequacy analysis shows lower percentage of heavy metal substance in each depth level compared to standard limit stated by the Ministry of Environment, along with low concerns raised after the analysis on heavy metal content of the spilled water on the concrete roads. Meanwhile, Korean Weigela (Weigela subsessilis L.H. Baily) was found to be withered in small-scale landscape trees planting sites. Among the seeding plants. the family of leguminosae, Silene armeria, Dendranthema boreale, Caryopteris incana and Aster yomena show good establishment results. Overall studies on planting of small and large landscape trees, planting method of container plants, planting method of ground cover plants, and germination and development trend of seeding plants of the experimental restoration site on abandoned concrete roads are revealing specific trends in the way landscape woody plants establishment and growth. Finally, this study suggests further studies and survey on varied plant restoration methods on abandoned concrete-roads for developed design guidelines of their methods.